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Citations for Lonza :
4851 - 4900 of 4910 citations for Rat Nuclear Factor Of Activated T Cells 5 NFAT5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Mononuclear cells were isolated with Ficoll-hypaque (γ = 1.077, GE Health) and red blood cells were lysed using ammonium-chloride-potassium (ACK) lysis buffer (Lonza, MD, USA). The remaining mononuclear cells were seeded in 150 x15 mm style non-tissue culture-treated petri dishes or non-tissue culture-treated 24-well plates at 1 ×106 cells/mL ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 × 105 single cells were resuspended with Lonza P3 Primary Cell Nucleofector® Solution and mixed with the pre-formed RNP complexes and transferred to a Nucleocuvette™ (Lonza). Nucleofection was performed using program EO-115 on the 4D Nucleofector™ X unit (Lonza) ...
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A modular CRISPR screen identifies individual and combination pathways contributing to HIV-1 latencybioRxiv - Microbiology 2022Quote: ... crRNPs for each gene of interest were generated as described previously in the Knockout Cell Clones and Pools section except that supplemented P3 buffer (Lonza; V4SP-3096) is used instead of SE buffer ...
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bioRxiv - Neuroscience 2022Quote: ... The cells were cultivated together with mixed glial cells plated on the bottom of 12-well plates for 7 days in EBM-2 medium (Lonza, Basel, Switzerland) containing 15% PDS ...
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bioRxiv - Cell Biology 2024Quote: ... monocytes were cultured in 24-well plates (Labclinics, Barcelona, Spain) at a concentration of 106 cells/ml in either X-VIVO 15 media (Lonza, Basel, Switzerland) or RPMI 1640 media (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... The transfection of chromaffin cells with the plasmids described above was performed by electroporation in an Amaxa Nucleofector 4D (Lonza, Cologne, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: The NK-92 cell line was acquired from the American Type Culture Collection and cultured in X-VIVO 10 (Lonza, 04-380Q) supplemented with 20% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2024Quote: 1 µL of 63 µM Alt-R™ S.p.Cas9 V3 (IDT: 10007807) was diluted with 18 µL of SE Cell Line Nucleofector® Solution (Lonza V4XC-1032), followed by the addition of 1.8 µL of 100 µM sgRNA (3:1 sgRNA:Cas9) ...
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bioRxiv - Microbiology 2023Quote: ... Extracellular bacteria were removed by incubation with fresh cell culture medium supplemented with 30 µg/ml gentamicin sulphate (Lonza BioWhittaker, Basel, Switzerland) for 15 min at 37°C/5% CO2 ...
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bioRxiv - Microbiology 2023Quote: ... 2□μl of each RNP complex were then mixed with the cell suspension and transferred into a 16-well reaction cuvette of the 4D-Nucleofector System (Lonza #V4XP-3032). The CD4+ T cells and CD34+ cells were electroplated using the programs EH-100 and DZ-100 ...
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bioRxiv - Microbiology 2023Quote: Vero C1008 (Vero 76, clone E6, Vero E6 [ECACC 85020206]) cells were seeded with DMEM (BE12-604F, Lonza Group AG (Basel, Switzerland)) from stocks in Costar□ 96-well clear tissue culture-treated flat bottom plates (353072 ...
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bioRxiv - Cell Biology 2023Quote: ... The culture dish was coated with 0.2% gelatin and the cells were cultured in endothelial growth medium (EGM)-2 complete media (#CC-3162, Lonza Clonetics, Fisher scientific) with EGM-2 SingleQuots supplement kit (CC-4176 ...
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bioRxiv - Immunology 2023Quote: ... 10% FBS heat-inactivated and 1% Penicillin/Streptomycin. Human Small Airway Epithelial Cells (SAECs, Cat. CC-2547) and the corresponding medium were commercially obtained by Lonza (NJ, USA). Human Pulmonary Microvascular Endothelial Cells (HPMECs ...
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bioRxiv - Cell Biology 2023Quote: ... MCF10A cells (female human mammary epithelial cells) were purchased from ATCC (CRL-10317) and cultured as recommended by ATCC in MEGM (Lonza CC-3150) supplemented with bovine pituitary extract ...
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bioRxiv - Microbiology 2024Quote: One million K562 cells were electroporated with 3 μg of DENV2-luciferase replicon [38] (provided by Jan Carette, Stanford University) in 100 μL SF Cell Line Nucleofector solution (Lonza V4XC-2012) using pulse code FF-120 (Amaxa 4D Nucleofector ...
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bioRxiv - Cell Biology 2024Quote: ... were obtained from Stephen Elledge’s laboratory at Harvard Medical School73 and cultured in MEGM™ Mammary Epithelial Cell Growth Medium (Lonza CC- 3150). In microscopy experiments we used the same media but without phenol red to reduce background fluorescence (Lonza CC-3153 phenol-red free basal media supplemented with growth factors and other components from the Lonza CC4136 kit) ...
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bioRxiv - Biochemistry 2024Quote: The lung type-2 epithelial cell line A549 (ATCC, Cat# CCL-185TM) was cultured in Ham’s F12 medium (Lonza Biosciences, Cat# 12615F) containing 10% heat-inactivated fetal calf serum (FCS ...
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bioRxiv - Synthetic Biology 2024Quote: ... Nucleofector program FF/120/DA was used to electroporate K562 cells with the pDNA mix on the Amaxa HT Nucleofector System (Lonza, AAU-1001). After electroporation ...
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bioRxiv - Cell Biology 2024Quote: Human umbilical vein endothelial cells (HUVEC) were purchased from ATCC (Manassas, VA, USA) and cultured in EGM-2 Endothelial Cell Growth Medium-2 BulletKit (Lonza, Basel, Switzerland) at 37°C with 5% CO2 and 95% air conditions until passage 5 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... RNA complex and Alt-R HDR Donor Oligos were transfected into HCT116 cells by electroporation (Lonza Nucleofector 96-well Shuttle System; Lonza, Bend, OR) using parameters provided by the manufacturer ...
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bioRxiv - Cell Biology 2020Quote: ... cells were serum starved for at least 13 h in 1% FBS (Atlanta Bio selected, Cat #S11110) or without FBS using endothelial cells growth basal medium (Lonza cat#cc-3121) instead of MCDB-131-complete media ...
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bioRxiv - Neuroscience 2019Quote: A human neuroglioma H4 cell-derived cell line stably over-expressing αSyn from a tetracycline inducible promoter20 was grown in serum-free X-VIVO media (Lonza Group, Basel, Switzerland). Cells were seeded in 96-well plates at 100K cells per well ...
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bioRxiv - Immunology 2021Quote: ... Vero cells were acquired from the American Type Culture Collection (ATCC #CCL-81; Manassas, VA, USA) and cultured in DMEM (Lonza, Walkersville, MD, USA) containing 2% FBS (SAFC Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... The solution was transferred into a certified cuvette and cell electroporation was conducted using Nucleofector® program X-013 (Amaxa® Nucleofector®, Lonza). Electroporated cells were immediately resuspended in pre-warmed phenol-free Leibovitz’s-L-15 medium (Fisher ...
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bioRxiv - Cancer Biology 2021Quote: ... human astrocytoma CCF-STTG1 (ATTC CRL-1718) and human glioblastoma cell line U87-MG (ATCC HTB-14) purchased from Lonza (Lonza Walkersville. Inc.) and American Type Culture Collection (ATCC) ...
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bioRxiv - Genomics 2021Quote: ... 23 uL of the cells-RNP-ssODN mixture were added to each well of a 96 well electroporation cuvette plate (Lonza, Cat #VVPA-1002), and nucleofected using the pulse code EH-115 ...
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bioRxiv - Cell Biology 2020Quote: ... Conditioned media (containing EVs) was removed and the cells washed x 2 with PBS before trypsinisation using 1 × Trypsin-EDTA (Lonza, UK cat: T3924). Cell counts and viability were checked at the time of EV harvest using the trypan blue exclusion assay (0.4% Trypan blue solution ...
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bioRxiv - Cancer Biology 2021Quote: ... and CT-26 (ATCC #CRL-2638) 4T1 cells were cultured in DMEM or RPMI 1640 with L-Glutamine (Lonza™ BioWhittaker™ # BW12115F12) supplemented with 10% of FBS ...
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bioRxiv - Molecular Biology 2021Quote: ... All sgRNA encoding oligonucleotides were inserted into a retroviral U6-sgRNA-PGK-GFP expression vector61 using a BsmBI restriction site and transfected into cells by Amaxa II electroporator (Lonza; program G-016) and Amax II Cell Line Nucleofector Kit (R ...
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bioRxiv - Microbiology 2022Quote: ... Each pellet was carefully resuspended in 20 μL of SF Cell Line Nucleofector Solution with Supplement 1 (Lonza Cat. #V4XC-2032, Basel, Switzerland) and 1.5 μL of pX458 plasmids (150 ng each of BAX g2 ...
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bioRxiv - Neuroscience 2023Quote: ... were purchased from the American Type Culture Collection (Tau biosensor cells; ATCC® CRL-3275™) and cultivated in complete Dulbecco’s Modified Eagle’s growth medium (Lonza, Cat. # 12–604F) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Immunology 2024Quote: ... including the use of 2 mM EDTA in PBS as the wash buffer and red blood cell lysis with ACK lysis buffer (Lonza by Thermo Fisher). Subsequently ...
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bioRxiv - Neuroscience 2023Quote: ... The RNP and HDR donor was delivered to MIN-6 cells by electroporation using the Lonza 4D-Nucleofector (Lonza Group AG, Basel, Switzerland), using the 96-well SE reagent kit and program CM-150 ...
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bioRxiv - Cell Biology 2022Quote: ... Bronchosphere differentiation media consists of 50% BEBM (CC-3171) and 50% DMEM/High Glucose supplemented with Bronchial Epithelial Cell Growth Medium SingleQuots™ (Lonza CC-4175).
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bioRxiv - Cancer Biology 2023Quote: ... Cells were nucleofected using program DS137 and buffer P3 on the 4D- Nucleofector system (4D-Nucleofector X unit, Lonza, cat. no. AAF-1003X). Pre-warmed complete culture medium supplemented with IL-7 (10ng/ml ...
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bioRxiv - Cancer Biology 2023Quote: PEO1 cells were maintained in RPMI+ 10% heat inactivated FBS (Avantor, Cat. #10803-034) + 100 μg/mL Penicillin/Streptomycin (Lonza, Cat. #09-757F). EHMT1 or EHMT2 overexpression cells were obtained by using lentivirus to infect cells with either TRE_EHMT1_hygromycin or TRE_EHMT2_hygromycin ...
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bioRxiv - Molecular Biology 2023Quote: Human primary pulmonary artery ECs (HPAECs) were obtained from ATCC and grown on gelatin-coated dishes in Endothelial Cell Basal Medium-2 (Lonza, Catalog # CC-3156) supplemented with EGM-2 SingleQuots Supplements (Lonza ...
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bioRxiv - Microbiology 2023Quote: ... cells were seeded at 1-3 × 104 cells/cm2 whilst in the log phase of replication with Dulbecco’s minimal essential medium (DMEM) (BE12-604F, Lonza Group AG (Basel, Switzerland)) supplemented with 9% foetal bovine serum (FBS ...
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bioRxiv - Genomics 2023Quote: ... the cells were pelleted at 100x g for 10 minutes and resuspended in room temperature Lonza P3 buffer (Lonza, catalog no. V4XP-3032) at 1.5x106 cells per 20 ul P3 ...
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bioRxiv - Cancer Biology 2024Quote: ... D2.A1 cells stably expressing luciferase (a gift from Dr. William Schiemann, Case Western Reserve University) were cultured in DMEM media (Lonza, catalog# BW12-604F) supplemented with 10% (v/v ...
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bioRxiv - Genomics 2024Quote: Approximately twenty-four hours after viral transduction the cells were centrifuged at 100g for 10 minutes and then resuspended in room temperature Lonza P3 electroporation buffer (Lonza, Cat #V4XP-3032) at 1-2E6 cells per 17.8 µL ...
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bioRxiv - Genomics 2024Quote: ... 23 uL of the cells-RNP-ssODN mixture was added to each well of a 96 well electroporation cuvette plate (Lonza, Cat #VVPA-1002), and nucleofected using the pulse code EH-115 ...
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bioRxiv - Neuroscience 2024Quote: Human neuroblastoma (SH-SY5Y) cells with inducible expression of α-synuclein 51 were grown in Roswell Park Memorial Institute (RPMI) 1640 medium (Lonza, #BE12-702F) supplemented with 15% fetal bovine serum (Biowest ...
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bioRxiv - Cell Biology 2024Quote: ... This mixture was incubated for 10 min-at room temperature and further diluted with 20 μL of P3 Primary Cell solution (Lonza, Walkersville, MD, USA). For the nucleofection process ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1-5x105 iPSCs were resuspended in 20μL of P3 primary cell nucleofector solution plus supplement (cat.: V4XP-3032; Lonza Bioscience, Walkersville, MD, USA) along with the RNP complex and nucleofected using Lonza 4D Nucleofector (program CA-137) ...
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bioRxiv - Bioengineering 2021Quote: ... were subsequently integrated into the AAVS1 locus of cells expressing rTetR-VP48 using TALEN-mediated homology-directed repair as follows: 1.0×106 K562 cells were electroporated in Amaxa solution (Lonza Nucleofector 2b, setting T0-16) with 1000 ng of reporter and 500 ng of each TALEN-L (Addgene #35431 ...
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bioRxiv - Microbiology 2021Quote: African green monkey kidney epithelial Vero cells (ATCC® CCL-81) and Vero E6 cells (ATCC® CRL-1586) were maintained in Dulbecco’s modified Eagle’s medium (12-604F, Lonza, BioWhittaker®, Walkersville, MD, USA) supplemented with 10% Fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2021Quote: ... Plasmids (10 μg) were then linearized with NotI and transfected into SmOx P9 TbGPI2-KO cells using a 4D nucleofector system (Lonza Group AG, Basel, Switzerland) with program FI-115 ...
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bioRxiv - Genetics 2020Quote: ... Reporter cell lines were generated by TALEN-mediated homology-directed repair to integrate a donor construct into the AAVS1 locus as follows: 1.2×106 K562 cells were electroporated in Amaxa solution (Lonza Nucleofector 2b, setting T0-16) with 1000 ng of reporter donor plasmid (Table S2) ...
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bioRxiv - Genetics 2021Quote: Human embryonic microglia clone 3 (HMC3, ATCC® CRL3304™) cells were cultured in minimum essential medium (MEM) Eagle-EBSS with NEAA without L-Glutamine (Lonza, Cologne, Germany) supplemented with 10% fetal bovine serum (Sigma-Aldrich ...