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Citations for Lonza :
4701 - 4750 of 4910 citations for Rat Nuclear Factor Of Activated T Cells 5 NFAT5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... 5.0-8.0 × 105 BTAG cells were electroporated with 3 μg of each gRNA using Amaxa 4D nucleofector (Lonza, V4XP-3012) and the CA-137 program ...
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bioRxiv - Molecular Biology 2023Quote: ... HeLa and SH-SY5Y cells were grown in Dulbecco’s modified Eagle medium with L-glutamine and 4.5 g/L glucose (DMEM; Lonza or Biowest) supplemented with 10% fetal calf serum (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... 1.5-2.0 x 105 cells were electroporated with 200 pmol of protein in 20 µl cuvettes using a 4D nucleofector device (Lonza) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2023Quote: ... All sorted and parental cell lines were confirmed to be mycoplasma-negative in a MycoAlert PLUS assay (Lonza, LT07-710) performed by the Koch Institute High-Throughput Sciences Facility (Cambridge ...
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bioRxiv - Cell Biology 2023Quote: ... This plasmid and plasmid pSPCas9(BB)-2A-puro(PX459)-hMyo10 above were transfected together into HeLa cells using an Amaxa nucleofection apparatus (Lonza). Five days later the cells were subjected to single-cell sorting into 96-well plates ...
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bioRxiv - Genomics 2023Quote: ... 5 µg of the 4sgRNA plasmid or 10 µM synthetic guide RNAs were mixed and the cell/reagent/nucleofection mix was transferred to Nucleofection cuvette strips (Lonza). Cells were electroporated using a 4D nucleofector (4D-Nucleofector Core Unit ...
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bioRxiv - Genetics 2023Quote: ... the assembled ribonucleoprotein complexes were added to non-attached primary hepatocytes in suspension at 1x106 cells / mL in HCM media (Lonza). Cells were incubated with rocking at 37°C for 2 hours before transplantation ...
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bioRxiv - Immunology 2023Quote: ... at bead-to-cell ratio of 1:1 in human IL-2 medium (X-VIVO 15, serum-free hematopoietic cell medium, with 2mM L-Glutamine and gentamicin (Lonza) supplemented with 5% Human AB serum (Valley Medical) ...
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bioRxiv - Bioengineering 2023Quote: ... were expanded in type I collagen precoated culture flasks in endothelial cell growth medium-2 (EGM-2) BulletKit medium (CC-3162, Lonza). Culture flasks were precoated with 50 μg ml−1 collagen I rat tail (354236 ...
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bioRxiv - Biochemistry 2023Quote: ... All cell lines were regularly monitored and tested negative for the presence of mycoplasma using a commercial biochemical test (MycoAlert, Lonza).
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bioRxiv - Cancer Biology 2022Quote: ... Kp27MYCER and B95-8) and adherent cell lines (HeLa, HEK293T) were grown in RPMI-1640 or DMEM basal media (Lonza) respectively supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2022Quote: ... the Raji cells were transduced with mcDNA-CD19-CAR by using preset program DS104 of 4D-Nucleofector™ system (Lonza). Electroporation was performed according to the manufacturer’s instruction ...
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bioRxiv - Genetics 2023Quote: ... Samples of supernatant media from cell culture experiments were analyzed monthly for the presence of mycoplasma using MycoAlert PLUS (Lonza).
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bioRxiv - Immunology 2023Quote: ... The volume of blood was determined and red blood cells were removed using ammonium-chloride-potassium (ACK) lysis buffer (Lonza) prior to antibody staining for flow cytometry analysis.
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bioRxiv - Systems Biology 2023Quote: ... The isolated CD14+ cells were cultured in Dulbecco′s Modified Eagle′s Medium (BE12-614F, DMEM, BioWhittaker, Lonza, Basel, Switzerland) supplemented with 10 % human AB+ serum (pooled from five healthy donors) ...
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bioRxiv - Genomics 2023Quote: ... purified Percoll-enriched mature schizonts (∼20 μl packed volume) were suspended in 100 μl of P3 primary cell solution (Lonza) containing 60 μg of linearized repair plasmid DNA (repair plasmid 1 and 2 separately in two separate transfection experiments ...
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bioRxiv - Molecular Biology 2023Quote: ... 1×105 cells were grown in each well of 12 well culture plates in SkGM-2 BulletKit growth medium (Lonza) to >90% confluency under standard culture conditions of 37°C and 5% CO2 ...
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bioRxiv - Synthetic Biology 2023Quote: ... An array of 20µL droplets of cell-laden hydrogel solutions were pipetted between two 25x75mm glass slides that were thrice treated with GelSlick (Lonza) separated by 3D-printed 400μm insert ...
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bioRxiv - Neuroscience 2023Quote: ... and the ssODN (1 μl; stock 100 μM, IDT) in 20 μl of nucleofection buffer P3 (P3 Primary Cell NucleofectorTM Solution, Lonza) were nucleofected (program CA137 ...
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bioRxiv - Biophysics 2023Quote: ... Directed differentiation of iPSCs into cardiomyocytes was triggered at 80–90% cell density by adding the RPMI 1640 medium (Lonza) containing B27 supplement minus insulin (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... 2μgs of the PARP1-sgRNAR-eGFP plasmid was nucleofected into the U2OS PARP1-/-and OVCAR8 PARP1-/-cell line using the Nucleofector (Lonza). Cells were selected in 2μg/mL G418 for 10 days and GFP-positive cells were sorted and used for subsequent experiments ...
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bioRxiv - Cancer Biology 2023Quote: ... AS5 cells were maintained in EBM-2 Basal Medium supplemented with EGM-2 SingleQuots (CC-3162, Lonza, Walkersville, MD, USA). The EOMA ...
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bioRxiv - Microbiology 2023Quote: ... or 1×106 CEM-M7 Cas9 cells were transfected with the HiFi Cas9 Nuclease V3 (IDT)/gRNA complex (80 pmol/300 pmol) (Lonza) using a non-targeting or a GRN(5’-GCGATCCTGCTTCCAAAGATC-3’) ...
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bioRxiv - Immunology 2023Quote: Mature human 3D microvascular tissue models were grown by seeding human umbilical vein endothelial cells (HUVECs) (Lonza Cat# CC-2519) into a collagen/matrigel mixture as described82,91 ...
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bioRxiv - Immunology 2023Quote: ... A total of 5×106 cells per transgene were washed with phosphate-buffered saline (PBS) and resuspended in 100µl NucleofectorTM Solution V (Lonza, Germany) containing the expression vector (Fig ...
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bioRxiv - Molecular Biology 2023Quote: ... MRTF-WT and MRTF knockout in NIH 3T3 (MRTF-KO-1 and MRTF-KO-2) cells were cultured in DMEM (BE12-614Q, Lonza) supplemented with 10 % Fetal Bovine Serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: WT cells were transfected with the sgRNA-containing plasmids by electroporation with a Nucleofector device (Lonza; in Mirus transfection solution). Cells were plated in culture dishes and maintained for three days ...
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bioRxiv - Cell Biology 2023Quote: ... pPB plasmids and pCMV-mPBase(neo-) encoding piggyBac transposase were co-transfected into MDCK cells by electroporation with an Amaxa nucleofector (Lonza). Cells were selected with the medium containing the following antibiotics ...
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bioRxiv - Immunology 2023Quote: ... CTLs derived from LifeAct-GFP expressing pmel-1 mice were incubated with peptide-loaded and IFNγ-treated B16 cells cultured in phenol red-free DMEM (Lonza) supplemented with 10% FBS on stage at 37°C ...
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bioRxiv - Immunology 2023Quote: ... and cultured in 96-well culture plates (2×103 cells/well) with serum or EP-stimulated MDMs supernatants in X-VIVO 15 medium (Lonza) with 10 U/mL of IL-2 (PeproTech ...
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bioRxiv - Cell Biology 2023Quote: ... BM CD34+ cells from young healthy donors for functional assays were collected at San Raffaele Hospital or purchased from Lonza. For the phenotypic analyses the sample size was determined by the number of individuals for whom excess material was available ...
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bioRxiv - Cancer Biology 2023Quote: ... The resulting cells were transduced with the STAT regulatory reporter (4M67-GFP) (Wei et al., 2014) overnight in suspension in complete Mammary Epithelial Cell Growth Media (MEGM+) (Lonza, cat ...
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bioRxiv - Cancer Biology 2023Quote: For the multi-ome analysis in young versus aged human bone marrow, bone marrow cells from young donors (26-year-old female, and 24-year-old male) were purchased from Lonza, while bone marrow samples from aged donors (70 and 77-year-old females ...
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bioRxiv - Cell Biology 2023Quote: Pooled knockout cell lines were generated by electroporating Cas9-guide RNA ribonucleoprotein complexes into reporter K562 cells using an Amaxa 4D Nucleofector X Unit (Lonza) and the SF Cell Line 4D-Nucleofector X Kit S (Lonza) ...
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bioRxiv - Biochemistry 2023Quote: ... Sf21 cells seeded at 500,000 cells/mL were transfected with recombinant bacmid DNA to generate the initial baculovirus (P1) in Insect-XPRESSTM Protein-free Insect Cell Medium (Lonza). The P1 virus was amplified two times (P2 and P3 ...
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bioRxiv - Microbiology 2023Quote: ... Virus stocks were propagated in Vero E6 cells, viral stocks and tissue culture supernatants were checked for endotoxin (Johnson et al., 2005) and mycoplasma (MycoAlert, Lonza) (La Linn et al. ...
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bioRxiv - Bioengineering 2023Quote: ... Human umbilical vein endothelial cells (HUVEC) (cAP-0001, Angio Proteomie, USA) were suspended in EGM2 medium (CC-3162, Lonza, Switzerland) at a density of 1–2 × 107 cells/ml ...
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bioRxiv - Biophysics 2023Quote: ... Samples of supernatant media from cell culture experiments were analyzed monthly for the presence of mycoplasma using MycoAlert PLUS (Lonza).
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bioRxiv - Cell Biology 2024Quote: ... 100 µL aliquots (7 × 106 cells) were then transferred into 2 mm EP cuvettes and electroporated on an Amaxa Nucleofector I (Lonza). For A2780 cells ...
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bioRxiv - Microbiology 2024Quote: ... Human airway tracheobronchial epithelial cells (NHBE) isolated from airway specimens from four donors without underlying lung disease were provided by Lonza, Inc ...
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bioRxiv - Biophysics 2024Quote: ... 4×105 cells were mixed with the plasmid-SE solution and nucleofected with program CM-137 using a 4D-Nucleofector (Lonza).
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bioRxiv - Bioengineering 2024Quote: ... The transfection reaction was transferred into P3 Primary cell cuvette and electroporated with EO-115 program on a Lonza 4D Nucleofector (Lonza). The cells were rested in the cuvette without disturbing them for 15 minutes at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... CB HSCs and BM HSPCs were cultured for 48h and AML cells for 24h and then CRISPR/Cas9 RNP electroporation was performed using the 4D-Nucleofector (Lonza), chemically synthesized gRNAs (IDT ...
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bioRxiv - Microbiology 2024Quote: ... aspirate supernatant and cover the cell monolayer with 3ml per well of 1.5% Sekam ME Agarose (Lonza; Cat. No. 50011), 2X EMEM (quality Biological ...
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bioRxiv - Microbiology 2024Quote: ... 1 μl of isolated schizonts was mixed with 7 μl of DNA and 18 μl of P3 Primary Cell 4D-Nucleofector solution from Lonza. This mixture was then added to a well within a 4D-Nucleofector 16-well strip from Lonza and electroporated using the FI-115 program on the Amaxa Nucleofector 4D ...
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bioRxiv - Immunology 2024Quote: ... They were then transfected by nucleofection with 3 μg of plasmid DNA in 100 μl of Cell Line Nucleofector Solution V (Lonza) using the V-001 program (Amaxa Biosystems) ...
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bioRxiv - Developmental Biology 2024Quote: ... we proceeded with 50% Basal media (+10uh/ml HGF) and 50% SkGM™-2 Skeletal Muscle Cell Growth Medium (Lonza). Subsequently ...
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bioRxiv - Developmental Biology 2024Quote: ... Sorted cells expressing medium levels of fluorescence were expanded and frozen then representative lots of each cell line were thawed and tested for mycoplasma contamination using the MycoAlert assay (Lonza), and fungi and aerobic bacteria sterility by inoculating conditioned medium into trypticase soya broth (Sigma Aldrich) ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were thawed according to the supplier’s instructions and expanded in collagen-coated flasks in human stellate cell growth medium (Lonza, MCST250) supplemented with 50 μM 2-Phospho-L-ascorbic acid (Merck ...
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bioRxiv - Cell Biology 2024Quote: ... with a density of 1.5 x106 using a 1:70 inoculation from the V1 stock for 92 hours at 21°C in Insect Xpress Protein-free Insect Cell Medium (Lonza) supplemented with GlutaMAX (GIBCO ...