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Citations for Lonza :
4801 - 4850 of 4910 citations for Rat Nuclear Factor Of Activated T Cells 5 NFAT5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... and 120 pmol of in vitro synthesized gRNA were electroporated into 250,000 MCF7 cells with the P3 primary nucleofection solution (Lonza, V4XP-3024), using the DN-100 Lonza 4D-Nucleofector program ...
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bioRxiv - Genetics 2023Quote: All iPSC lines were passaged as clumps or single cells upon reaching 80-85% confluency using Versene (0.02% EDTA solution, Lonza #17-711E) or StemPro Accutase (Gibco #A1110501) ...
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bioRxiv - Microbiology 2023Quote: ... Pooled sgRNA and healing template were added to the cell suspension and nucleofected using a Lonza Nucleofector 2b (Lonza, Basel, Switzerland) using program X-014 ...
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bioRxiv - Cell Biology 2023Quote: ... and progenitors isolated from day 14 medium-sized CFU-M were suspended in Endothelial Cell Growth Medium (Lonza, Cat# CC-4542) and plated on growth factor-reduced MatrigelTM (BD Biosciences ...
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bioRxiv - Cell Biology 2022Quote: ... hVECs and pVECs were cultured in EGM™-2 MV Microvascular Endothelial Cell Growth Medium-2 BulletKit™ (Lonza, #CC-3202). hVICs and pVICs were cultured in VIC medium ...
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bioRxiv - Cancer Biology 2023Quote: The human glioblastoma NCH601 cells (Schuster et al., 2020) were cultured as non-adherent spheres in DMEM-F12 medium (Lonza: 12634010) containing 1X Bit-100 (Provitro ...
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bioRxiv - Microbiology 2023Quote: ... cells were transfected with pcDNA::3xflag-legC4 or pcDNA::3xflag vector (Table S2) using a Nucleofector 2b electroporator (Lonza, Basel, Switzerland) and cultured over 14 days with Zeocin selection (200 -1000 µg mL- 1) ...
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bioRxiv - Immunology 2023Quote: ... PBECs were scraped carefully from the luminal surface of the bronchus without disturbing the mucosal surface integrity and cultured in bronchial epithelial cell growth medium (BEBM; Lonza, Belgium) supplemented with antibiotics ...
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bioRxiv - Bioengineering 2023Quote: ... were cultured until ∼80% confluence before being passaged and seeded at 10,000 cells/cm2 in EGM-2MV (LONZA, Cat# CC-3202) in 6-well tissue culture plates and were allowed to adhere for 5 hours before induction of serum starvation in EBM-2 (LONZA ...
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bioRxiv - Cell Biology 2023Quote: ... Transfection in the appropriate cell lines was achieved by nucleofection using program X-014 of the AMAXA Nucleofector® apparatus (Lonza) as previously described (Burkard et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... Complete culture media for each cell line was as follow: FaDu and Detroit562 (Eagle’s minimal essential medium (EMEM) (Lonza, #12-125Q) with 10% fetal bovine serum ...
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bioRxiv - Cell Biology 2023Quote: Human umbilical vein endothelial cells from six independent donors (CC-2517/0000242397, C2517A/0000088928, C2517A/0000439577, C2517A/0000306191, CC-2517/0000198228, C2517A/0000699238: Lonza, TX) were used for transcriptomic analyses and validation ...
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bioRxiv - Genetics 2023Quote: ... The RNP complex together with 7 µg of donor DNA was nucleofected into 3 × 107 ISE6 cells using EN150 and buffer SF via the 4D-Nucleofector system (Lonza Bioscience) (Figure S1) ...
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A CRISPR activation screen identifies FBXO22 as an E3 ligase supporting targeted protein degradationbioRxiv - Biochemistry 2023Quote: ... MDA-MB-231 and PC3 cells with FBXO22 CRISPR-Cas9 knockout were generated through electroporation of Cas9-sgRNA ribonucleoprotein (RNP) complex using 4D-Nucleofector (Lonza Bioscience). Two sgRNAs targeting FBXO22 gene (FBXO22 sgRNA#1 ...
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bioRxiv - Cancer Biology 2024Quote: ... 20 µL of cell suspension (200,000 cells) was mixed with the RNP-containing solution and electroporated using the 4D-Nucleofector X Unit (Lonza, Basel, Switzerland) with the CM138 nucleofection program ...
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bioRxiv - Immunology 2024Quote: ... One million NK cells were resuspended in 20 ul 4D nucleofector master mix (82% P3 + 18% supplement 1; Lonza, V4XP-3032), and mixed with Cas9 RNP for electroporation using program CM137 ...
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bioRxiv - Cell Biology 2019Quote: ... Primary human Small Airway Epithelial Cells (CC-2547, Lot 501937) and primary human lung fibroblasts (CC-2512, Lot. 0000608197) were obtained from Lonza (Basel, Switzerland).
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bioRxiv - Cell Biology 2020Quote: ... The supernatants were pelleted at 350 xg for 10 min and resuspended in Endothelial Cell Growth Basal Medium-2 (EBM-2, CC-3156, Lonza, Morristown, NJ). Isolated NPCs were stained with SYTOX® Red (5uM ...
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bioRxiv - Developmental Biology 2020Quote: ... cell line WTC11 (gift from Bruce Conklin, available at NIGMS Human Genetic Cell Repository/Coriell #GM25236) (Miyaoka et al., 2014) was electroporated (Lonza #VPH-5012) with a cloned nuclease construct containing a guide RNA (sgRNA1 ...
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bioRxiv - Microbiology 2019Quote: ... Cells were resuspended in 25 uL of CRISPR/Cas9 crRNP complexes that were pre-assembled in P3 Primary Cell Nucleofector Solution (Lonza #V4SP-3096) before electroporation in a single well of a 96-well Nucleocuvette Plate using program EH-115 ...
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bioRxiv - Genomics 2020Quote: ... THLE-2 cells derived from primary human liver epithelial cells were obtained from ATCC (Cat. CRL-2706) and grown in BEGM medium (Lonza; Walkersville, MD) supplemented with 10% fetal bovine serum ...
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bioRxiv - Immunology 2022Quote: ... MV-4-11 (ATCC #CRL-9591), mBMDMs (were a kind gift from Linfa Wang, Duke-NUS, Singapore) and normal bronchial epithelial cells (NHBE, Lonza #CC-2541) were cultured according to manufacturer’s protocols ...
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bioRxiv - Cell Biology 2019Quote: ... The cell suspension was washed twice in Hepes buffer and resuspended in a HCM™ BulletKit™ medium (cat. CC-3198, LONZA) supplemented with 10% FBS ...
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bioRxiv - Physiology 2019Quote: ... and primary human pulmonary arterial smooth muscle cells (HPASMCs; product code. CC-2581, lot no. 029837, 407340, and 559495) were obtained from Lonza (Basel, Switzerland). All experimental and surgical procedures were approved by the Institutional Committee for the Use and Care of Laboratory Animals in Juntendo University (Tokyo ...
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bioRxiv - Neuroscience 2019Quote: ... Co-transfection of 500 ng sgRNA plasmid and 1 µl of 10 µM ssODN template into 2×105 HEK293 cells was performed using nucleofection on the 4D-Nucleofector system (LONZA, Basel, Switzerland), as described previously38 ...
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bioRxiv - Neuroscience 2020Quote: ... then transferred to a 15mL conical tube with 8mL pre-warmed Xeno-Free Neural Stem Cell Media (XF-NSM) consisting of X-Vivo Basal Medium (Lonza, 04-744Q), 1X N2 Supplement (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: Genes were knocked out using 1-2 synthetic sgRNAs (Synthego) in complex with spCas9 (Aldeveron 9214) that were electroporated into cells using a nucleofector system (Lonza V4XC- 1032) according to published methods (Hoellerbauer et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... Single cells from digested mammary glands were resuspended and plated on Collagen I-coated plates (50μg/ml) in stem cell-enriching medium MEBM (Lonza Cat. #CC-3151), supplemented with 2% B27 (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... human astrocytoma CCF-STTG1 (ATTC CRL-1718) and human glioblastoma cell line U87MG (ATCC HTB-14) purchased from Lonza (Lonza Walkersville. Inc.) and American Type Culture Collection (ATCC) ...
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bioRxiv - Cell Biology 2020Quote: ... The pPBbsr-based FRET biosensor and pCMV-mPBase (neo-) encoding the piggyBac transposase were co-transfected into MDCK cells using an Amaxa nucleofector system (Lonza, Basel, Switzerland) at a ratio of 4:1 ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were electroporated with 10 µg lineage recorder DNA and 1 µg Sleeping Beauty transposase in 100 µl Human Stem Cell nucleofection buffer (Lonza, VVPH-5022) using the B-016 program of the 4D-Nucleofector (Lonza ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 million NK cells were resuspended in 20 μl Amaxa 4D nucleofector master mix (82% P3 + 18% supplement 1) (Lonza, V4XP-3032), then mixed with Cas12a RNPs for electroporation using program CM137 ...
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bioRxiv - Molecular Biology 2021Quote: ... Spodoptera frugiperda Sf9 insect cells (kindly provided by P. Cejka) were grown at 27°C in serum free Insect-XPRESS medium (Lonza, BELN12-730Q).
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bioRxiv - Microbiology 2020Quote: ... ALI cultures were established from enzymatically dissociated human sinonasal epithelial cells (HSEC) as previously described (85, 86) and grown to confluence with bronchial epithelial basal medium (BEBM; Lonza, Alpharetta, GA) supplemented with BEGM Singlequots (Lonza) ...
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bioRxiv - Neuroscience 2021Quote: Dissociated neurosphere cells were electroporated with either CFP-IRES or Safb-IRES-CFP vector (4µg) using a 4D-Nucleofector (Lonza, program DS-112) in 16-well stripes (500,000 cells/well ...
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bioRxiv - Cancer Biology 2020Quote: Tumor cells (td-tomato MDA-MB-231 SORE6>GFP) were plated alone or with macrophages (BAC1.2F5) or HUVEC (Lonza, cat# CC-2517) at a 1:5 ratio (5,000 tumor cells and 25,000 macrophages or HUVEC ...
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bioRxiv - Physiology 2020Quote: Growth media used for the expansion of human muscle-derived cell populations consisted of Hams F-10 nutrient mix (Lonza, Basel, Switzerland) with added L-glutamine (2.5 mM) ...
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bioRxiv - Immunology 2021Quote: Primary BEAS-2B (ATCC® CRL-9609TM) (normal human airway epithelial cells) as well as all the basal media and growth supplements were obtained from Lonza (Walkersville, MD). Cells were cultivated according to the instructions of the manufacturer on plastic dishes or flasks (BD Bioscience ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were authenticated as recommended by AACR (http://aacrjournals.org/content/cell-line-authentication-information) and tested for the absence of mycoplasma using MycoAlert® (Lonza, Basel, Switzerland) or MycoFluor (Invitrogen ...
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bioRxiv - Molecular Biology 2019Quote: ... and cultured in endothelial cell basal medium-2 (EBM-2) supplied with EGM™-2 BulletKit™ (catalogue no. CC-5035; Lonza). HEK293A cells were purchased from Invitrogen (catalog no ...
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Extracellular matrix micropatterning technology for whole cell cryogenic electron microscopy studiesbioRxiv - Bioengineering 2019Quote: ... Madin-Darby Canine Kidney strain II (MDCKII) cells were transfected with Life-Act GFP using the Amaxa Biosystem Nucleofector II system (Lonza, VCA-1005) (Gift from Jens Moller ...
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bioRxiv - Cancer Biology 2019Quote: ... THP1 and NB4 cells (ATCC: CCL-243, TIB-202 and DSMZ: ACC 207) were maintained in RPMI 1640 (BioWhittaker, Lonza, Verviers, Belgium), 10% fetal bovine serum (FCS ...
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bioRxiv - Molecular Biology 2020Quote: ... Dharmacon) or non-targeting control pools (Horizon, Dharmacon) were introduced to H7 ESCs and HeLa cells using 4D-Nucleofector™ (Lonza™) electroporation as per manufacturer instructions for each cell line (ESC ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were maintained in RPMI 1640 medium supplemented with 2 mM Glutamine and 10% (v/v) heat-inactivated FBS (Lonza Group Ltd.) containing 100µg streptomycin ml−1 and 100 U penicillin ml−1 and passaged as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... and CDR3B gRNA (10 nmol) were used to transfect 1×108 TnT cells using the 4D-Nucleofector LV unit (Lonza, #AAF-1002L). TnT cells with restored CD3 surface expression were bulk-sorted (SEL 1 ...
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bioRxiv - Microbiology 2021Quote: ... were isolated by Ficoll density gradient centrifugation as described previously [22] and seeded at a density of 1×106 cells/cm2 in X-VIVO 15 medium (Lonza, Cologne, Germany) supplemented with 10% (v/v ...
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bioRxiv - Immunology 2019Quote: Primary human normal airway tracheobronchial epithelial cells from de-identified donors (NHBE) (sex: male and female) were provided by Lonza (Walkersville, MD) and grown in BEM media supplemented with the BEGM bullet kit (Lonza) ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were washed 1x in RT PBS and transfected with ∼15μg of DNA plasmid using Amaxa nucleofector 2b (Lonza, program O-005). After transfection ...
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bioRxiv - Biochemistry 2021Quote: ... 105 cells were mixed with a plasmid vector (AsCas12a, n-SpCas9 (D10A, H840A)) and 20 μl of electroporation buffer (Lonza, V4XC-2032) and were nucleofected according to the manufacturer’s instructions (program ...
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bioRxiv - Molecular Biology 2021Quote: ... Myoblasts derived from an adult DM1 patient biopsy (DM-05 (Xia et al., 2013)) were cultured in SkGM-2 skeletal muscle cell growth medium (Lonza, #CC-3245).