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Citations for Lonza :
4651 - 4700 of 4910 citations for Rat Nuclear Factor Of Activated T Cells 5 NFAT5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... and soluble anti-CD28 (500 ng/mL) at a density of 2 × 106 cells/mL of X-vivo 15 serum-free medium (Lonza). For iTreg differentiation ...
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bioRxiv - Immunology 2021Quote: A single-cell suspension was prepared from spleen and lymph nodes and red blood cells were removed by lysis with ACK buffer (Lonza). CD4+ T cells were enriched using microbeads (Miltenyi Biotec ...
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bioRxiv - Microbiology 2021Quote: ... The human lung epithelial cell line Calu-3 (ATCC HTB-55) was maintained in DMEM F-12 (Lonza, Basel, Switzerland) supplemented with 10% FBS ...
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bioRxiv - Molecular Biology 2021Quote: ... human endothelial colony-forming cell-derived endothelial cells (ECFC-EC) are isolated from cord blood via selection for the CD31+ cell population and cultured in EGM2 medium (Lonza). Normal human lung fibroblasts (LF ...
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bioRxiv - Immunology 2021Quote: Spleens and cervical lymph nodes were isolated from 6-10 weeks old C57B6 mice and homogenized through a sieve in B cell culture medium (RPMI-1640 (Lonza), 10% fetal calf serum (FCS ...
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bioRxiv - Microbiology 2021Quote: African green monkey kidney epithelial BSC-1 cells (ATCC CCL-26) were maintained in Eagle’s minimal essential medium (EMEM; Lonza, Inc.) containing 5% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2022Quote: ... and the spheres were collected by inverting the strainer into a new conical tube and rinsing with hematopoietic cell media consisting of X-VIVO 15 (Lonza) supplemented with 1X Pen/Strep (Thermo-Fisher Scientific) ...
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bioRxiv - Developmental Biology 2022Quote: 3 × 105 cells in 20 µL transfection buffer were mixed with 1µg plasmids and electroporated with a 4D nucleofector (Lonza Bioscience) following the preset protocol for K562 ...
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bioRxiv - Immunology 2022Quote: ... was prepared by mixing 250 nmol target gene sgRNA+ 50 nmol eGFP sgRNA+ 50 nmol SpCas9 2NLS Nuclease (Synthego) in 50 μL mouse B cell nucleofection buffer (Lonza) at RT for 30min ...
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bioRxiv - Cancer Biology 2022Quote: ... Primary prostate cells established from fresh male radical prostatectomy tissues were isolated as previously described and cultured in prostate cell growth medium (Lonza). All cells were cultured at 37°C and 5% CO2 ...
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bioRxiv - Bioengineering 2022Quote: ... MDA-MB-231-rtTA-Antares cells were then electroporated in 20ul format using 4D-Nucleofector using CH-125 protocol in SF buffer (Lonza) with 1 μg PiggyBac transposon:transposase plasmid mixture (2:1 PB-gvpA:PB-gvpNV transposons ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were plated at 1 × 104/well in black 96-well plates using Bio Whittaker HBSS (BE10-527F, Lonza, Switzerland) with 4.5 g/L glucose ...
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bioRxiv - Cancer Biology 2022Quote: ... and resuspended in a mixture of 16.4 µl SF cell line solution and 3.6 µl supplemental solution-1 (Lonza, V4XC-2032). The sgRNA and Cas9 ribonuclease protein complex under incubation was then mixed with the cell suspension and 20 µl of it was put a cuvette and nucleofected using 4D-Nucleofector (Lonza ...
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bioRxiv - Cancer Biology 2022Quote: ... The sgRNA and Cas9 ribonuclease protein complex under incubation was then mixed with the cell suspension and 20 µl of it was put a cuvette and nucleofected using 4D-Nucleofector (Lonza) with the inbuilt program DS-137 for cell lines and EO-115 for primary T cells ...
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bioRxiv - Biochemistry 2022Quote: ... Baculoviral P3 stock was used to infect Sf9 insect cells at 1.5 × 106 cells·mL-1 in Insect-XPRESS media (Lonza #12-730Q) supplemented with 2% FBS (Capricorn #FBS-12A) ...
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bioRxiv - Bioengineering 2022Quote: ... Bone marrow mononuclear cells were plated in endothelial growth medium 2 + 10% fetal bovine serum (FBS) (EGM-2MV, Lonza, Switzerland) on fibronectin coated plates and cultured at 37°C in 5% CO2 incubators ...
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bioRxiv - Immunology 2022Quote: All the tumor cells used in the paper tested negative for mycoplasma (MycoAlert®, Lonza and MycoSensor qPCR®, Agilent). The metastatic melanoma cells were cultured in RPMI 1640 (Corning ...
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bioRxiv - Microbiology 2022Quote: ... was added to the resuspended cells and nucleofected using the CM-150 program on the 4D-Nucleofector X Unit (Lonza). Nucleofected cells were allowed to recover in a 37°C incubator with 5% CO2 for 20 minutes ...
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bioRxiv - Microbiology 2022Quote: ... Nuli-1 cells were cultured on Collagen IV coated plates as previously described in Bronchial Epithelial Cell Growth Media (BEGM) with supplements (Lonza) (Zabner et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 µg of digested plasmid was added to the bottom of a 1.5 mL tube and 800,000 cells suspended in 20 µL SE nucleofection solution (16.4 µL nucleofection solution, 3.6 µL nucleofection supplement, Lonza, V4XC-1012). The solution was then transferred to a well in a 16-well Nucleocuvette™ Strip and nucleofected in a Lonza 4D-Nucleofector™ running the CM-104 program for U-2 OS cells ...
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bioRxiv - Molecular Biology 2022Quote: ... and one adult cell line lablled J (59 year old, Caucasian female, catalogue number: CC-2511, lot number: #693503) purchased from Lonza.
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bioRxiv - Molecular Biology 2023Quote: ... freshly harvested spleens of 12-13 weeks-old female NOD mice with early diabetes were filtered through a nylon mesh by followed lysis of the red blood cells with ACK Lysing buffer (Lonza). After starvation ...
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bioRxiv - Immunology 2022Quote: ... 1×106 cells were electroporated (pulse code EH100) with Cas9 ribonucleoprotein and DNA templates in 20 ul Nucleofector Solution P3 (Lonza) with a 4D Nucleofector X unit (Lonza) ...
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bioRxiv - Immunology 2022Quote: ... 4.4 μl gRNA-Cas9 complex was added to a 20 μl cell suspension in P3 buffer and transferred into a nucleovette (Lonza), placed on Amaxa Nucleofector and 96 well Shuttle (Lonza ...
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bioRxiv - Physiology 2022Quote: ... and HaCaT cells (ATCC, Manassas, VA, USA) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; Lonza Group Ltd., Basel, Switzerland) supplemented with 100 U/ml penicillin ...
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bioRxiv - Bioengineering 2022Quote: ... scaffolds were transferred to 24-wells plates and kept overnight in endothelial cell growth medium-2 (EGM-2, CC-3162, Lonza). HUVECs were seeded the next day.
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bioRxiv - Cancer Biology 2024Quote: ... Cells were seeded at a density of 7500 cells in 6-cm3 tissue culture dish in duplicate in 0.8% SeaPlaque GTG agarose (Lonza 50111) mixed with Iscove’s Modified Dulbecco’s medium (Gibco 12200-036 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were confirmed to be mycoplasma-free based on a luminescence-based enzymatic assay (MycoAlert®, Lonza, Cat# LT07-118) conducted within 1 week of the commencement and completion of the experiments ...
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bioRxiv - Biochemistry 2023Quote: ... 200,000 H1299 cells were resuspended in the resulting solution containing ribonucleoprotein complexes (RNPs) and electroporated using a 4D-Nucleofector (Amaxa, Lonza). Nucleofected cells were then expanded and single-cell cloned by limiting dilution by plating 0.5 cells/well in a 96 well plate ...
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bioRxiv - Neuroscience 2023Quote: ... Transfection of neuronal cells with pCAG-CaBLAM and pCAG-GeNL(Ca2+)_480 was done by electroporation using an Amaxa Nucleofection Device (Lonza) at day-in-vitro 0 (DIV0) ...
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bioRxiv - Cancer Biology 2024Quote: ... The OCI-LY3 cell line was cultured in IMDM with 25 mM HEPES and 2 mM L-glutamine (Lonza; Switzerland), supplemented with P/S ...
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bioRxiv - Cell Biology 2024Quote: ... The inoculum was removed two hours post infection and cells were covered with 0.4% SeaPlaque™ agarose (Lonza Group AG) in medium ...
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bioRxiv - Molecular Biology 2024Quote: ... Retrovirally transfection with a MSCV-MLL/AF9-IRES-NeoR construct in sorted progenitor cells was performed a day after incubation in RPMI1640 (Lonza) transduction medium containing 10% (FBS ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were passaged 1:10 every 3-4 days by trypsinisation and were regularly screened for mycoplasma contamination (Mycoalert, Lonza).
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bioRxiv - Immunology 2024Quote: Ten micrograms of expression plasmid or 20 pmol of siRNA were introduced into 1 x 107 BMDC cells by electroporation using a Nucleofector 2b (Lonza) with an Amaxa Mouse Dendritic Cell Nucleofection Kit (Lonza) ...
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bioRxiv - Immunology 2024Quote: ... The reaction contained 400 pmol of Cas9 protein and 1 × 107 of the transduced NK cells in 100 µL of P3 solution (Lonza). Immediately after electroporation ...
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bioRxiv - Immunology 2024Quote: ... They were then transfected by nucleofection with 3 μg of plasmid DNA in 100 μl of Cell Line Nucleofector Solution V (Lonza) using the V-001 program (Amaxa Biosystems) ...
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bioRxiv - Bioengineering 2024Quote: ... are commonly used as an experimental model for human hearts due to their anatomical and structural similarities and convenient availability.36–38 Cells were cultured on tissue culture treated flasks with Endothelial Growth Medium-2 (EGM-2, Lonza), which was changed every other day until the cells reached ∼75% confluence ...
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bioRxiv - Cancer Biology 2023Quote: ... AS5 cells were maintained in EBM-2 Basal Medium supplemented with EGM-2 SingleQuots (CC-3162, Lonza, Walkersville, MD, USA). The EOMA ...
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bioRxiv - Cell Biology 2023Quote: ... BM CD34+ cells from young healthy donors for functional assays were collected at San Raffaele Hospital or purchased from Lonza. For the phenotypic analyses the sample size was determined by the number of individuals for whom excess material was available ...
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bioRxiv - Cell Biology 2023Quote: Pooled knockout cell lines were generated by electroporating Cas9-guide RNA ribonucleoprotein complexes into reporter K562 cells using an Amaxa 4D Nucleofector X Unit (Lonza) and the SF Cell Line 4D-Nucleofector X Kit S (Lonza) ...
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bioRxiv - Immunology 2023Quote: ... CTLs derived from LifeAct-GFP expressing pmel-1 mice were incubated with peptide-loaded and IFNγ-treated B16 cells cultured in phenol red-free DMEM (Lonza) supplemented with 10% FBS on stage at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... MCF 10A cells were obtained from ATCC (Cat# CRL10317) and were grown in mammary epithelial growth media (Cat# CC-3150, Lonza) supplemented with cholera toxin (Cat# C8052 ...
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bioRxiv - Cell Biology 2023Quote: BCi-NS1.1 cells (passage 17-25) (kind gift of Dr. Ronald Crystal) and Normal Human Bronchial Epithelial cells (NHBE) (cat. no. CC-2541, Lonza, lot numbers 623950 ...
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bioRxiv - Molecular Biology 2023Quote: ... the paired RNPs were combined and nucleofected into 4×105 Raw 264-7 cells suspended in 10 ul of nucleofection buffer (Lonza) using the program DS-136 in a 4D Nucleofector X Unit (Lonza) ...
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bioRxiv - Immunology 2022Quote: ... Three-five million cells were combined with 200 pMol siRNA (siControl or siME1) in 20 μl P3 nucleofection media (Lonza) per well of the 16-well Nucleocuvette strips (X unit) ...
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bioRxiv - Immunology 2022Quote: ... Red blood cells from the collected blood or from the single cell suspension of the cardiac tissue were removed via treatment with a hypotonic lysis buffer (Lonza). Cells were then washed and the following antibodies were used for flow cytometry ...
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bioRxiv - Cancer Biology 2023Quote: Cell and organoid lines were generated from C3-TAg tumors (supplementary file 1) and tested for mycoplasma (Lonza, LT07-703) prior to use and the creation of frozen stocks ...
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bioRxiv - Biophysics 2023Quote: ... Directed differentiation of iPSCs into cardiomyocytes was triggered at 80–90% cell density by adding the RPMI 1640 medium (Lonza) containing B27 supplement minus insulin (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... 2μgs of the PARP1-sgRNAR-eGFP plasmid was nucleofected into the U2OS PARP1-/-and OVCAR8 PARP1-/-cell line using the Nucleofector (Lonza). Cells were selected in 2μg/mL G418 for 10 days and GFP-positive cells were sorted and used for subsequent experiments ...