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Citations for Lonza :
4501 - 4550 of 4910 citations for Rat Nuclear Factor Of Activated T Cells 5 NFAT5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Aliquots of 4 · 105 cells were transferred into individual wells of 16-well or 96-well nucleofection cuvettes (Lonza), combined with 20 µL pre-formed RNP or nucleofector solution (no RNP control) ...
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bioRxiv - Microbiology 2021Quote: Primary human bronchial epithelial cells (HBECs) derived from a non-smoking donor (Cat# CC-2540, male; Lonza; Donor 1) or derived directly from a patient at Cambridge University Hospitals NHS Trust (Research Ethics Committee Reference 19/SW/0152 ...
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bioRxiv - Microbiology 2021Quote: Human airway tracheobronchial epithelial cells isolated from airway specimens from donors without underlying lung disease were provided by Lonza, Inc ...
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bioRxiv - Microbiology 2021Quote: VeroE6 (ATCC® CRL 1586TM) wildtype and retrovirally-transduced cell lines were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Lonza) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2020Quote: ... was passaged once in embryonated chicken eggs followed by two passages in Madin-Darby Canine Kidney (MDCK) cells (ATCC) in Eagle’s minimal essential medium (EMEM; Lonza) supplemented with 100 IU ml-1 penicillin-100 µg ml-1 streptomycin mixture (Lonza) ...
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bioRxiv - Cell Biology 2021Quote: Tracheal cells (60,000-100,000 cells/ well in 96 well plate) were cultured in small airway growth media (SABMTMBasal Medium, CC-3119 Lonza) containing 1× insulin ...
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bioRxiv - Cell Biology 2022Quote: Human umbilical vein endothelial cells (HUVEC) were cultured between passage 3 and 6 in EGM-2 complete medium (Lonza)3,52–55 ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting vector was used to generate a D.mel-2 stable cell line adapted to grow in suspension in serum-free Insect-Xpress medium (Lonza). In brief ...
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bioRxiv - Cell Biology 2022Quote: ... together with a single-stranded donor (ssODN) were delivered into mito-GFP1-10 cells using the Nucleofector system (Lonza). Cell line constitutively expressing mito-mCherry-GFP1-10 was generated by transducing ARPE-19 cells with lentiviral mito-mCherry-GFP1-10 and enriched for mCherry positive cells via cell sorting ...
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bioRxiv - Cell Biology 2022Quote: ... 1.5 μg ssODN and 1.2 μl 100 μM Alt-R Cas9 Electroporation Enhancer (IDT) were mixed and added to 20,000 cells resuspended in 18 μl SF Nucleofection Buffer (Lonza). Nucleofection mixture were transferred to 16-well Nucleocuvette strips (Lonza ...
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bioRxiv - Cancer Biology 2022Quote: ... HEK293T cells (human embryonic kidney; LGC Nordic ATCC CRL-3216) were cultured in DMEM high glucose (#BE12-614Q, Lonza), supplemented with 2 mM L-glutamine ...
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bioRxiv - Cell Biology 2022Quote: BEAS-2B cells (ATCC, Manassas, VA, USA) were cultured in Bronchial Epithelial Basal Medium (BEBM; Lonza, Walkersville, MD, USA) supplemented with the Bronchial Epithelial Growth Bullet Kit (BEGM ...
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bioRxiv - Immunology 2022Quote: ... PBMC were seeded at 1.5×106 cells per cm2 in X-VIVO 10 media (Cat. No. BE04-743Q, Lonza) +1% penicillin-streptomycin (Cat ...
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bioRxiv - Microbiology 2022Quote: ... and neonatal dermal microvascular endothelial cells (hDMVEC) were maintained as monolayer cultures in EBM-2 medium (Lonza, Walkersville, USA) supplemented with 5% fetal bovine serum ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: NIH-3T3 cells were grown in complete NIH-3T3 media (DMEM with 4.5g/L Glucose and 4mM L-Glutamine (Lonza), 10% ...
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bioRxiv - Microbiology 2022Quote: ... NHBE cells from a single donor were amplified and differentiated into air-liquid interface cultures as recommended by Lonza and described in (49) ...
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bioRxiv - Neuroscience 2023Quote: ... The two RNP complexes were then combined and diluted in 20 μL of P3 Primary Cell Nucleofector Solution (Lonza). To electroporate the Cas9 RNP complexes into hiPS cells ...
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bioRxiv - Systems Biology 2022Quote: DMECs were plated on poly-L-lysine-coated 6-well glass-bottom plates at 8,450 cells per cm2 in either BPM or growth medium (EGM2-MV, Lonza). After 24 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... once the cell confluency reached 90% the culture medium was changed to 10 mL XVIVO-10 medium (Lonza #(BE)BP04-743Q ...
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bioRxiv - Molecular Biology 2024Quote: ... 33 μL of cell suspension was mixed with 66 μL of solution 1 (0.83% low-melting-point agarose SeaPlaque GTG (Lonza), 170 mM sorbitol ...
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bioRxiv - Cancer Biology 2024Quote: ... and resuspended in 20 µL of SF Cell Line Nucleofector™ Solution with the supplement added (Lonza V4XC-2032). The cells and the RNP complex were carefully mixed and transferred to a well of the 16-well Nucleocuvette™ ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were nucleofected with the DT-130 program using the 4D-Nucleofector system with Core and X unit (Lonza). Nucleofected cells were incubated for 10min at room temperature before being transferred to a 6-well plate containing pre-warmed and equilibrated medium (DMEM + 10% FBS) ...
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bioRxiv - Molecular Biology 2024Quote: ... 33 μL cell suspension was mixed with 66 μL of solution 1 (0.83% low-melting-point agarose SeaPlaque GTG (Lonza), 170 mM sorbitol ...
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bioRxiv - Bioengineering 2024Quote: ... Human primary bone marrow MSCs derived by plastic adherence of mononucleated cells from human bone marrow aspirate donors (Lonza) and were cultured in α-minimal essential medium (αMEM ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell lines were regularly authenticated by STR profiling (LabCorp, Burlington, NC) and tested for mycoplasma (MycoAlert, Lonza #LT07-218). To create a chronic CuHiexposure model ...
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bioRxiv - Cell Biology 2024Quote: ... All cell lines were tested for mycoplasma at each batch freezing by PCR (32) and biochemical assay (MycoAlert, Lonza).
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bioRxiv - Cell Biology 2023Quote: ... after which the mixture was transferred to an electroporation cuvette and cells were electroporated in a 2b-Nucleofector (Lonza). Finally ...
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bioRxiv - Bioengineering 2024Quote: Commercially available primary adult human mesenchymal stem cells (hMSCs) from Donor 47506 were used (Lonza Group Ltd., Basel, Switzerland). Cells were cultured at passage 3 and seeded in triplicate in hMSC Growth Medium (MSCGM™ Mesenchymal Stem Cell Growth Medium ...
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bioRxiv - Bioengineering 2024Quote: hMSC were plated at 15,000 cells/well in 24-well plates and grown overnight in a growth medium containing 10% FBS (Lonza). When cells reach 80% confluency ...
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bioRxiv - Cancer Biology 2024Quote: ... Human Umbilical Vein Endothelial Cells (HUVECs) were cultured in complete EGM-2 (cat# CC-3162, Lonza, Allendale, NJ, USA) and were not used beyond passage five for any experiments.
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bioRxiv - Bioengineering 2024Quote: hMSC were plated at 15,000 cells/well in 24-well plates and grown overnight in a growth medium containing 10% FBS (Lonza). Once cells reached 80% confluency ...
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bioRxiv - Immunology 2023Quote: ... different tumor cell lines were stained with carboxyfluorescein succinimidyl ester (CFSE) following the manufacture’s protocols and cultured in X-vivo (Lonza) supplemented with 5% FBS (Gbico ...
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bioRxiv - Bioengineering 2023Quote: ... gRNAs were complexed with recombinant Cas9 (IDT Cat#1081059) and introduced into cells by electroporation (4D-Nucleofector, Lonza Biosciences) according to manufacturer protocols ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 µM electroporation enhancer (IDT)) into 2 x 105 KPC cells on a Nucleofector 4D (Lonza Biosciences, Walkersville, MD) with an SF kit (Catalog ...
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bioRxiv - Genomics 2023Quote: 200,000 PUER cells were transfected with 500ng each of Cas9 and donor vector in SF buffer (Lonza, V4SC-2096) using program CM134 of the 4D-Nucleofector (Lonza) ...
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bioRxiv - Bioengineering 2023Quote: ... single guide hybrids were mixed with 3uM Cas9 nuclease (Berkeley Labs) at a 1.2:1 ratio and delivered to cells by Lonza 3D (CA-137 ...
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bioRxiv - Biochemistry 2023Quote: ... DNA (10 μg) was transfected into SmOx P9 cells using a 4D nucleofector system (Lonza Group AG, Basel, Switzerland) with program FI-115 ...
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bioRxiv - Bioengineering 2023Quote: ... each device was seeded directly on the PREDICT96-ALI membrane in the apical chamber with 10,000 cells in Small Airway Epithelial Growth Medium (Lonza) containing 100 U/mL penicillin–streptomycin (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2023Quote: ... cells (used for TT-TL seq and ChIP-seq spike-ins) were cultured in Schneider’s modified Drosophila medium (Lonza) supplemented with 10% FBS at 27°C without CO2 ...
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bioRxiv - Cancer Biology 2023Quote: SK-N-BE (2) (ATCC: no. CRL-2271) cells were cultured in Dulbecco’s Modified Eagle Medium (Lonza, Cat# BW12614F), supplemented with heat-inactivated FBS (Hyclone ...
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bioRxiv - Cancer Biology 2023Quote: ... 2×10E6 ME-1 cells were nucleofected with CRISPR/Cas9 plasmids (2 μg each) using Nucleofector Technology (Lonza Biologics) with the program X-01 and Amaxa Cell Line Nucleofector Kit V ...
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bioRxiv - Cancer Biology 2022Quote: ... The B16 and the human melanoma cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Lonza; BE12-604F/U1) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2022Quote: Normal human bronchial epithelial cells (HBECs) from 3 independent donors (Supplemental Table S1) were obtained from Lonza (CC-2540) and cultured in BEGM growth media (Lonza CC-3170) ...
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bioRxiv - Bioengineering 2023Quote: ... The cells were first thawed and seeded on a T150 flask containing bronchial epithelial growth medium (Lonza CC-3170) for 2D cell culture growth ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell lines were routinely verified via STR genotyping and tested for mycoplasma contamination using the Lonza MycoAlert assay (Lonza). Below is a list of cell line details:
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bioRxiv - Immunology 2023Quote: Low passage primary human bronchial epithelial cells (HBEC) from healthy adult donors were obtained commercially (Lonza, Walkersville, MD, USA) and cultured at air-liquid interface according to the manufacturer’s instructions (Stem Cell Technologies ...
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bioRxiv - Microbiology 2023Quote: Human airway tracheobronchial epithelial cells isolated from airway specimens from donors without underlying lung disease were provided by Lonza, Inc ...
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bioRxiv - Immunology 2023Quote: ... The sgRNA complexes were then added to 1×106 NK92 cells resuspended in 16uL of P3 nucleofection buffer (Lonza). The nucleofection mixture was transferred to a 16-well strip for nucleofection in the Lonza 4D Nucleofector using the pulse code CM-138 ...
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bioRxiv - Cell Biology 2023Quote: Endogenously edited Halo-ATG2A cells expressing WT ATG9A and KO were transfected with HA-6x-ubiquitin-eBFP2 by LONZA electroporation using standard settings for U2OS cells and a homemade buffer of RPMI supplemented with 50mM bicarbonate ...
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Self-organised pattern formation in the developing neural tube by a temporal relay of BMP signallingbioRxiv - Developmental Biology 2023Quote: A total of 3-4µg of plasmid DNA was electroporated into 3-4x106 early passage ES cells using the Amaxa Nucleofector II (Lonza) programme A-023 ...