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Citations for Lonza :
4451 - 4500 of 4910 citations for Rat Nuclear Factor Of Activated T Cells 5 NFAT5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: RPE-1 cells were transfected with 20 pmol siRNA by nucleofection (Amaxa Lonza 4D program EA-104 P3 solution) or Lipofectamine RNAiMAX transfection reagent (ThermoFisher ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... CT26wt and C51wt cells were transfected with the A33 gene in pcDNA3.1(+) using the Amaxa™ 4D-Nucleofector (Lonza) and the SG Cell Line 4D-Nucleofector® X Kit L (Lonza ...
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bioRxiv - Cancer Biology 2021Quote: ... hTERT-HME1 (ATCC CRL-4010) were cultured in Mammary Epithelial Cell Basal Medium (MEBM) supplemented accordingly to manufacturers (LONZA). HCC1954 cells (ATCC CRL-2328 ...
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bioRxiv - Developmental Biology 2021Quote: ... 2 × 105 organoid single cells were re-suspended with Lonza P3 nucleofection buffer and 1 µl of pmaxGFP (Lonza) and transferred to 20 µl nucleofection cuvette (V4XP-3024 ...
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bioRxiv - Genetics 2020Quote: ... THP1-CAS9 cells were nucleofected with sgRNA using a protocol recommended for THP1 (Lonza, Amaxa 4D Nucleofector, Protocol #292). Cutting efficiency for each guide was measured using GeneArt Genomic Cleavage Detection Kit (ThermoFisher ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were thawed and cultured according to the manufacturer’s instructions using the recommended hepatocyte culture media provided by Lonza. Hepatocytes were seeded at a density of 2×106 cells per well in a 6-well plate coated with collagen type I (Corning ...
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bioRxiv - Molecular Biology 2021Quote: The expression plasmids for the HA-V5-FLAG-dCas9 bait and the dCas9-VPR were delivered to J-Lat 11.1 cells by nucleofection using Amaxa Nucleofector (Lonza) as previously described (3 ...
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bioRxiv - Cancer Biology 2021Quote: MG-63 cells (1000 per well, 24-well plate) were seeded into 0.4% low melting point agarose (Lonza, 50101) on top of a 1% agarose layer ...
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bioRxiv - Microbiology 2020Quote: ... Stock titer was determined by plaque assay (described below) on HeLa cells overlaid with MEM (Lonza, Walkersville, MD, USA) + 0.5% agarose (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... EBs were collected and transferred into 6-well cell culture plate (12-16 EBs/well) in X-VIVO15 (Lonza) supplemented with IL-3 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Vero cells were plated at 2.5 x 105 cell/well in 24-well plates in Essential-modified Eagle Medium (EMEM, Lonza) supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Cell Biology 2021Quote: ... Infections for plaque assay in Vero E6 cells were performed in Eagle’s minimal essential medium with 25 mM HEPES (EMEM; Lonza) supplemented with 2% FCS ...
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bioRxiv - Cell Biology 2021Quote: Vero cells were transfected with 2 µg plasmid DNA in the Amaxa Nucleofector solution R (Lonza Bioscience, Rockville, MD), following the manufacturer’s instructions with program V-01 ...
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bioRxiv - Cell Biology 2021Quote: ... siRNA transfected primary XP186LV (XP-C patient cells) were serum-deprived for at least 24 hours in Ham’s F10 (Lonza) containing 0.5% FCS and antibiotics to arrest cells in G0 ...
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bioRxiv - Bioengineering 2022Quote: ... HUVECs were obtained from Lonza™ and cultured in Endothelial Cell Growth Medium-2 (EGM-2, PromoCell® C-22111 or Lonza™ CC-3162). Human primary pericytes were purchased from PromoCell® (PromoCell® ...
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bioRxiv - Neuroscience 2022Quote: ... The BBB working medium was prepared using EGM-2 Endothelial Cell Growth Medium-2 BulletKit (Lonza, cat. #CC-3162), which includes EBM-2 Endothelial basal medium and endothelial growth supplements hEGF ...
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bioRxiv - Immunology 2022Quote: Mammalian cells used in this study include BSC-40 (ATCC CRL-2761) (in Dulbecco minimal essential medium, DMEM, Lonza), healthy human periphery CD14+ monocytes (Lonza ...
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bioRxiv - Immunology 2022Quote: ... and human monocytic leukemia cell line THP-1 (ATCC TIB-202) were cultured in RPMI-1640 (Lonza, 12-115F) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2022Quote: ... 50 000 to 100 000 cells per reaction was then resuspended in 20uL of P3 solution (Lonza, V4XP-3032) and mixed by pipetting with the full gRNA-Cas9 complex mix previously assembled then added in the electroporation chamber well (Lonza ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were maintained in culture for the indicated time in the following medium: X-VIVO 10 (Lonza, BE04-380Q) supplemented with 20% BIT 9500 Serum Substitute (StemCell technologies ...
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bioRxiv - Microbiology 2020Quote: ... cells were nucleofected using Nucleofector SE solution and reagent into Nucleocuvette following manufacturer’s instructions using 4D-Nucleofector System (Lonza). The cell number and nucleic acid amounts are indicated in each figure legends ...
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bioRxiv - Immunology 2020Quote: ... DCs were seeded in a 24-well plate at a density of 1× 106 cells/well on 1.5mm coverslips (Warner Instrument) and cultured in complete phenol-red free RPMI 1640 media (Lonza) for 30 minutes at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... or ds-UM DNA oligos (IDT) (Table S4) were nucleofected into mouse ES cells using an Amaxa nucleofector (Lonza) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... CD3+ T cells were debeaded with an EasySep magnet (STEMCELL) prior to resuspension and electroporation at 0.5-1×106 cell mL−1 in P3 buffer (Lonza). Following electroporations with RNPs and HDRTs ...
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bioRxiv - Bioengineering 2021Quote: ... Approximately 1.5e6 K562 cells were electroporated in a final volume of 20 µL in a 16-well nucleocuvette strip (Lonza). For the T-cell experiments ...
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bioRxiv - Immunology 2022Quote: ... The solution was centrifuged at 400G for 5 minutes at 4°C and the cell pellet was resuspended in 1mL of Hank’s Balanced Salt Solution (HBSS; Lonza) containing 1% BSA (Sigma Aldrich ...
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bioRxiv - Immunology 2022Quote: NIH3T3 WT fibroblast cells (3T3) and human CD40L-expressing 3T3 (Urashima et al., 1996) were cultured in IMDM (Lonza) containing 10% FCS (Bodinco) ...
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bioRxiv - Microbiology 2022Quote: ... NHBE cells were infected with viruses at an MOI of 0.01 and cultured in B-ALI growth medium (Lonza) at 33°C ...
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bioRxiv - Developmental Biology 2019Quote: ... were nucleofected into 1×106 pluripotent RUES2 cells using the B-016 setting on an Amaxa Nucleofector II (Lonza). Nucleofected cells were then plated as per maintenance conditions ...
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bioRxiv - Molecular Biology 2019Quote: ... were obtained from Lonza (CC-2581) and maintained for no more than 8 passages in Smooth Muscle Cell Growth Media (SM-GM2, Lonza) supplemented with 5% FBS and 1X Penicillin/Streptomycin (Gibco) ...
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bioRxiv - Genetics 2019Quote: ... Human multipotent adipose-derived stem cells (hMADS) were cultured in growth medium which consisted of: DMEM (Lonza, BE12-707F), 10% FBS ...
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bioRxiv - Molecular Biology 2019Quote: ... and wild-type J1 male mouse embryonic stem cells were grown on 0.1% gelatin-coated dishes in DMEM (Lonza) complemented with 10% (v/v ...
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bioRxiv - Microbiology 2021Quote: ... MD Cat# CC-2540) and maintained in Bronchial Epithelial Cell Growth Medium (BEGM) (Lonza, Walkersville, MD, Cat# CC-3170) with all provided supplements in the BulletKit ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and the cell pellet was transfected with 1.0 μg of cheriff eGFP using a Nucleofector IIS (Lonza, Basel, Switzerland) and Amaxa Basic Neuron SCN Nucleofector Kit (VSPI—1003 ...
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bioRxiv - Biochemistry 2021Quote: ... DNA (10 μg) was transfected into SmOx P9 cells using a 4D nucleofector system (Lonza Group AG, Basel, Switzerland) with program FI-115 ...
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bioRxiv - Cancer Biology 2020Quote: 1×105 cells were suspended in 1 ml of 0.33% select agar in small airway growth medium without EGF (Lonza) and plated on a bottom layer of 0.5% select agar in the same media in six-well dishes ...
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bioRxiv - Cell Biology 2019Quote: ... Cells (1 × 106/10 cm dish) were cultured in Dulbecco’s Modified Eagle Medium (DMEM; catalog no. 12-604F, Lonza) with 20% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2019Quote: ... HPCs were transfected using the Amaxa 4D system and the Primary Cell P3 solution according to the manufacturer’s instructions (Lonza). HPCs were transfected with 1ug pSIREN plasmid DNA per 106 cells using either program EH-100 or EO-100 ...
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bioRxiv - Cell Biology 2020Quote: ... BEAS-2B was cultured in BEGM™ Bronchial Epithelial Cell Growth Medium BulletKit™ (Lonza Catalog No. CC-3170). TALL-104 T cells were from the ATCC (ATCC® CRL-11386™ ...
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bioRxiv - Immunology 2021Quote: Growth medium for Rabbit Kidney (RK13) cells (CCL-37, ATCC) consisted of Dulbecco’s Minimum Essential Medium (12-614F, Lonza) supplemented to 10% fetal bovine serum (A3160401 ...
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bioRxiv - Bioengineering 2021Quote: ... These cells were then seeded at densities as above in ultra-low adherent round-bottom 96-well plates (Lonza) for 60 hours ...
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bioRxiv - Immunology 2021Quote: ... 2×106 cells were nucleofected with 2μg of pcDNA3.1+ plasmid per reaction (Lonza-AMAXA program X-001, Nucleofector 2b). 24 hours after transfection ...
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bioRxiv - Microbiology 2021Quote: ... cells on filter sections were immobilized by embedding in 0.1% low-gelling agarose (MetaPhor™, Lonza, Rockland, ME, USA). CARD-FISH was performed using a HRP-labeled oligonucleotide probe specific for Nitrososphaeria (HRP-labeled Thaum726 [GCTTTCATCCCTCACCGTC] and unlabeled competitors [Thaum726_compA ...
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bioRxiv - Genomics 2021Quote: ... Human chronic myeloid leukemia HAP1 cells (Horizon™ C859) were cultivated in Iscove’s Modified Dulbecco’s Medium (IMDM) with 20% fetal bovine serum (Lonza) and 1% penicillin-streptomycin (Gibco) ...
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bioRxiv - Cancer Biology 2021Quote: ... MSC used as control were passaged and maintained in MSCGM Mesenchymal Stem Cell Growth Medium BulletKitTM (PT-3001, Lonza). All steps were performed at 37°C.
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bioRxiv - Cell Biology 2020Quote: ... Eriksson’s lab) and human osteosarcoma (U2OS) (ATCC HTB-96) cells were cultured in high-glucose DMEM (Lonza, BE12-119F), supplemented with 10% FBS (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... In PRNT, Vero E6 cells (Vero C1008, ATCC CRL 1586) were cultured in Eagle’s minimal essential medium (EMEM, Lonza) supplemented with 5% FBS (GIBCO) ...
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bioRxiv - Immunology 2021Quote: ... cells were washed with PBS and 1 ml of fresh Hepatocyte Culture Medium (HCM) was added per well (Lonza). At 6 or 72 hpi ...
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bioRxiv - Immunology 2021Quote: ... washed in PBS by centrifugation for 5 min at 1300 rpm at 4°C and resuspended at 4.0 × 106 cells/ml in complete medium (IMDM 2 mM glutamax I supplemented with 8% heat-inactivated FCS (Lonza), 2% heat-inactivated chicken serum ...
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bioRxiv - Microbiology 2021Quote: Human airway tracheobronchial epithelial cells isolated from airway specimens from donors without underlying lung disease were provided by Lonza, Inc ...