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Citations for Lonza :
4601 - 4650 of 4910 citations for Rat Nuclear Factor Of Activated T Cells 5 NFAT5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2022Quote: The ability of CTEPH ECs to form tube-like structures on basement membrane matrix was compared to control human pulmonary artery endothelial cells (PAECs) obtained commercially (Lonza). The Cultrex® Reduced Growth Factor Basement Membrane Matrix (Trevigen cat.no ...
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bioRxiv - Immunology 2022Quote: ... IFNAR KO A549 cells were generated by CRISPR-Cas9 ribonucleoprotein (RNP) complex (IDT) transfection using the Nucleofector system (Lonza Bioscience). Specifically ...
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bioRxiv - Immunology 2022Quote: ... The digested vessels were then centrifuged and suspended in EGM™-2 Endothelial Cell Growth Medium-2 BulletKit™ (LONZA) supplemented with GlutaMax (ThermoFisher ...
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bioRxiv - Genetics 2022Quote: ... Cells were dissociated using accutase and 1 x 106 cells in P3 buffer were mixed with the RNP/template complex and nucleofected in large cuvettes (V4XP-3024 Lonza) using 4D-Nucleofector on program CA137 ...
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bioRxiv - Cell Biology 2022Quote: ... HEK-293T and U-2OS (ATCC, Manassas, Virginia) cells were grown in Dulbecco’s modified Eagle’s medium (DMEM; Lonza, Basel, Switzerland) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2022Quote: ... and NP-C1 patient fibroblasts (GM18393 and GM17919A) were purchased from the Coriell Cell Repository (Table S1) and cultured in DMEM (Lonza) supplemented with 20% FBS (Bovogen) ...
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bioRxiv - Cell Biology 2022Quote: ... human astrocytoma CCF-STTG1 (ATTC CRL-1718) and human glioblastoma cell line U87-MG (ATCC HTB-14) purchased from Lonza (Lonza Walkersville ...
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bioRxiv - Bioengineering 2022Quote: ... Human hepatic stellate cell LX-2 was cultured in HEPES-buffered Dulbecco’s modified Eagle medium (DMEM) (Lonza BioWhittaker, Verviers, Belgium) supplemented with 4 mmol/L L-glutamine (Lonza) ...
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bioRxiv - Genomics 2022Quote: ... hiPSC-derived epicardial cells were dissociated with Accutase and plated at a density of 104 cells/cm2 in 6-well plates and cultured in fibroblast growth medium (Lonza) supplemented with 20 ng/ml FGF2 and 10 μM SB431542 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were seeded on protein-coated wells (9.4 x 104 cells/cm2) in either AimV/SF medium (FN-null MEFs) or Small Airway Epithelial Basal Medium (CC-3119; Lonza) in the absence or presence of EDTA (10 mM) ...
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bioRxiv - Immunology 2020Quote: ... 5 µg of NOSIP shRNA plasmids or control TRC2 plasmid was mixed with 100 µl Cell Line Nuceleofector Solution V (Lonza) and added to one million cells ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HUVECs were cultured in Endothelial Cell Growth Medium-2 BulletKit (EGM-2, cat. no. CC-3162, Lonza Inc., ME, USA). THP-1 cells were cultured in RPMI-1640 medium ...
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bioRxiv - Microbiology 2020Quote: ... for Vero-118 cells and serum reduced (2%) DMEM supplemented with 100 IU/ml penicillin-100 μg/ml streptomycin mixture (Lonza), 2 mM L-glutamine (Lonza) ...
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bioRxiv - Microbiology 2021Quote: Constructs co-expressing full-length hACE2 or cACE2 with mCherry2 protein (CMV promoter-ACE2-IRES-mCherry2) were generated and transfected into MDCK-SIAT1 cells via electroporation with Lonza Nucleofector system (Lonza) using the manufacturer’s protocol with program A024 ...
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bioRxiv - Immunology 2020Quote: ... Cells were then counted and frozen at 10×106 cells/ml of freezing media (10% DMSO in fetal bovine serum) (Lonza). and stored in liquid nitrogen until further use.
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bioRxiv - Bioengineering 2021Quote: ... SK-N-BE(2) or H460 cells were seeded at densities mentioned above in ultra-low adherent round-bottom 96-well plates (Lonza) for 72 hours ...
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bioRxiv - Immunology 2022Quote: ... Lysate were pellet (500 g 8min) and red blood cells were lysate with 500 µl of ACK lysing buffer (Lonza) for 5min ...
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bioRxiv - Cancer Biology 2022Quote: ... LSECs were counted and 105 cells were seeded in a 48-well plate and cultured in collagenated plates with EGM-2 medium (Lonza) for 3 days ...
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bioRxiv - Immunology 2019Quote: ... The superior right lung lobe was mashed through a cell strainer and treated with Ammonium-Chloride-Potassium (ACK) lysis buffer (10-5483, Lonza). Cells were pelleted ...
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bioRxiv - Biophysics 2019Quote: ... Directed differentiation of iPSCs into cardiomyocytes was triggered at 80-90% cell density by adding the RPMI 1640 medium (Lonza) contained B27 supplement minus insulin (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... and a guide RNA targeting Tp53 (Wang et al., 2015) were transfected into cells using a Nucleofector 2b Device (Lonza) with the setting T023 ...
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bioRxiv - Bioengineering 2019Quote: Human umbilical vein endothelial cells (HUVECs) were purchased form Lonza (C2519A) and cultured in EGM-2 media (Lonza; CC-3162). HUVECs and Stage 6 clusters containing SC-β cells were dispersed with TripLE Express and plated into a 6-well plate on an Orbi-Shaker at 100 rpm ...
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bioRxiv - Bioengineering 2020Quote: DNA delivery was carried out on 20 μL volume format using Amaxa 4D-Nucleofector System and Cell line nucleofector solution SF (Lonza). Total amount of DNA electroporated was 3 μg (1.5 μg each plasmid in the case of using two plasmids at the same time) ...
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bioRxiv - Microbiology 2019Quote: ... In vitro transcribed RNA derived from wt or mutant pEAN900 was electroporated into BHK-21 cells using Amaxa Nucleofector technology (Lonza), as described previously (Beerens and Snijder ...
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bioRxiv - Molecular Biology 2020Quote: ... pelleted at 350 xg for 10 minutes and resuspended in EBM-2 Basal Medium (CC-3156, Lonzs, Basel, Switzerland) supplemented with Microvascular Endothelial Cell Growth Medium-2 SingleQuots Supplements (CC-4147, Lonza) and 15% fetal bovine serum ...
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bioRxiv - Neuroscience 2020Quote: ... Effects of PI3K inhibition on Syk phosphorylation in mouse WT primary microglia was studied by plating 100 000 cells in 96-well plate in Dulbecco’s modified Eagle’s medium (DMEM, Lonza, Verviers, Belgium) supplemented with 2mM L-glutamine (Lonza ...
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bioRxiv - Immunology 2020Quote: 2×106 freshly isolated primary B cells were washed in PBS and resuspended in 20 μl P3 Primary Cell Nucleofector Solution buffer prepared with Supplement 1 buffer (Lonza) according to the manufacturer’s instructions (P3 Primary Cell 4D-Nucleofector X Kit S) ...
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bioRxiv - Cell Biology 2020Quote: Adipose-derived human mesenchymal stem cells (AD-hMSCs) and dermal fibroblasts (DF) used in this study were purchased from Lonza. AD-hMSCs and DF were isolated from a 42-year-old ...
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bioRxiv - Cancer Biology 2021Quote: ... Lung cancer cell lines (H3122, H2228, A549, H460, Calu-1, and H1437) were cultured in RPMI-1640 (Lonza, 15-1675) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2020Quote: Human aortic endothelial cells (HAEC, CC-2535) and human skeletal myoblasts (HSMM-Muscle Myoblasts, CC-2580) were obtained from Lonza Company ...
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bioRxiv - Immunology 2019Quote: ... were generated by seeding 2 x 106 BM cells in a 100 mm non-tissue culture treated dish in RPMI 1640 (Lonza) supplemented with 10% FCS ...
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bioRxiv - Molecular Biology 2021Quote: ... Bacmids were prepared by transposition of these plasmids and were used to transfect Sf9 cells in Insect Express media (Lonza) before viral amplification in the same cell line using standard techniques ...
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bioRxiv - Microbiology 2020Quote: ... cells were transfected with pcDNA3 HA eIF4GI plasmid or control green fluorescent protein (GFP)-expressing plasmid (pMAX-GFP plasmid, Lonza). pcDNA3 HA eIF4GI (1–1599 ...
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bioRxiv - Molecular Biology 2021Quote: ... Control pulmonary arterial smooth muscle cells were obtained from human lung tissues from normal donor lungs at the Cleveland Clinic (gift of Drs. Suzy Comhair and Serpil Erzurum) and from pooled primary cells from a commercial sources (Lonza).
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bioRxiv - Cell Biology 2019Quote: Mesenchymal stromal cells originating from the bone marrow of two healthy human donors were purchased from Lonza (Walkersville, MD, USA) and stored at −180°C in liquid nitrogen prior to experimentation ...
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bioRxiv - Cell Biology 2019Quote: ... 2 x 106 cells of MEFs were transfected with 1.2 nM of siRNAs using Amaxa Nucleofector II (Lonza, Basel, Switzerland) and MF 1 Nucleofector kit (Lonza ...
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bioRxiv - Molecular Biology 2019Quote: ... Primary HBECs (human bronchial epithelial cells) were grown in 2% oxygen using a 1:1 mixture of bronchial epithelial basal medium (BEBM; Lonza) and DMEM and supplemented with SingleQuot (Lonza ...
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bioRxiv - Cell Biology 2021Quote: ... 5,000 cells were plated in low-attachment 96-well plates containing 100 µL MEGM media (without BPE) (Lonza, CC-3150), 20 ng/mL FGF (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... 25,000 HMVEC and 50,000 Calu-3 cells were seeded per insert on either the apical or basolateral side as indicated and grown in EGM-Plus (Lonza), EGM-2MV (Lonza ...
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bioRxiv - Microbiology 2021Quote: ... HeLa (ATCC® CCL-2™), and HaCaT cells (gift from Jonathan Garlick, Tufts University) were grown in Dulbecco’s modified Eagle medium (DMEM; Lonza) containing high glucose ...
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bioRxiv - Cell Biology 2019Quote: HMEC FL2 cell line was cultured as previously described (Katajisto et al. 2015) in Mammary epithelial basal medium (MEGM; Lonza). For Laminin preadhesion assays ...
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bioRxiv - Cancer Biology 2021Quote: Human A549 lung adenocarcinoma cells obtained from ATCC (Manassas VA) were grown in Dulbecco’s Modified Eagle’s Medium (DMEM – Lonza, Wakersville MD) supplemented with 10% FBS superior (Biochrom ...
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bioRxiv - Microbiology 2021Quote: ... 2 µg pcDNA-pp71-flag and 1 µg of pBRep-Cre (Wolfram Brune, Hamburg, Germany) was electroporated into Fi301 cells using the Amaxa nucleofector program A-23 (Lonza). Plaque formation was checked over the course of 14 days ...
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bioRxiv - Microbiology 2021Quote: HEK293T-ACE2 cells (NR-52511, BEI Resources, Manasas, VA) were grown in Dulbecco’s Minimal Essential Medium (DMEM, Lonza, Walkersville, MD), supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Cancer Biology 2021Quote: ... Ker-CT cells were maintained in KGM-Gold Keratinocyte growth medium supplemented with KGM-Gold™ BulletKit™(Lonza #00192060). Cells were split when confluent ...
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bioRxiv - Biophysics 2021Quote: ... 2 μg of each Pcdh expression construct were transfected into 20 μL of the K562 cell suspension by electroporation using an Amaxa 4D- Nucleofector (Lonza). Transfected cells were transferred to a 24-well plate in 500 μL of medium per well and incubated overnight at 37°C and 5% CO2 ...
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bioRxiv - Cancer Biology 2021Quote: ... Stable LBH-overexpressing BT549 cell lines were generated by nucleofection of 2×106 BT549 cells with 2 μg linearized pCDNA3 or pCDNA3+Lbh 13 and 1 μg pEGFP (Lonza) in Solution V (Lonza) ...
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bioRxiv - Cancer Biology 2021Quote: Tumour cells were genetically edited by electroporating the Cas9/crRNA/tracrRNA or Cas9/sgRNA ribonucleoprotein (RNP) complexes into cells using the 4D-nucleofector system (Lonza). To prepare the guide RNA complex ...
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bioRxiv - Immunology 2021Quote: ... Cells were then nucleofected using a 4D-Nucleofector™ with X-Unit (V4XP-4032 and V4XP-9096, Lonza, Basel, Switzerland). Up to three RNP complexes in 9 µL were used per reaction ...
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bioRxiv - Immunology 2021Quote: ... The enriched CD4+CD25hi cells were cultured in 24-well non-tissue culture plates at 1 x 106 cells/mL in X-Vivo-15 (Lonza) supplemented with 10% heat-inactivated human AB serum (Sigma) ...