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Citations for Lonza :
4401 - 4450 of 4910 citations for Rat Nuclear Factor Of Activated T Cells 5 NFAT5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Cell lines were confirmed to be mycoplasma-negative in a MycoAlert PLUS assay (Lonza catalog number LT07-710) performed by the Koch Institute High-Throughput Sciences Facility (Cambridge ...
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bioRxiv - Cancer Biology 2023Quote: K562 cells containing deletions in ALAS2 or FECH were created using a multi-guide strategy via nucleofection (Lonza) of Cas9/RNP complexes (Gene Knockout Kit v2 ...
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bioRxiv - Immunology 2024Quote: ... All recovered cell fractions were rested overnight in EP-TICLE culture media consisting of X Vivo-15 (Lonza) supplemented with 2% KnockOut serum replacement (Gibco) ...
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bioRxiv - Immunology 2024Quote: ... All cell lines used were confirmed to be negative for mycoplasma by annual testing using MycoAlert Plus (Lonza). Last testing was performed on 14 August 2019.
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bioRxiv - Immunology 2022Quote: ... used for baculovirus preparation were maintained in Insect-XPRESSTM Protein-free Insect Cell Medium with L-Glutamine (Lonza) at 28°C under agitation ...
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bioRxiv - Molecular Biology 2023Quote: ... The RNP mixture was added to cells resuspended in nucleofection solution along with 0.5 ug pmax-GFP (Lonza) and 1 uL electroporation enhancer (100 uM ...
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bioRxiv - Molecular Biology 2023Quote: Human embryonic kidney 293 (HEK293) cells (ATCC; CRL-1573) were grown in high-glucose DMEM (Lonza; BE12-604F) supplemented with 10% foetal bovine serum (FBS ...
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An indocyanine green-based liquid biopsy test for circulating tumor cells for pediatric liver cancerbioRxiv - Cancer Biology 2023Quote: ... cells were kept in culture for 96 hours in complete hepatocyte basal medium (cat. no. CC-3198, Lonza). Cells were then fixed in 4% paraformaldehyde (cat ...
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bioRxiv - Cell Biology 2023Quote: ... Two days after transfection of WT HeLa cells with pSpCas9(BB)-2A-GFP-ghMyo10 using Amaxa nucleofection (Lonza), GFP-positive cells were subjected to single-cell sorting into 96-well plates using a BD FACS cell sorter ...
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bioRxiv - Genomics 2023Quote: 2 × 105 HCT116-Cas9 and iPSC-iCas9 were resuspended in 20 µl SE cell line nucleofection solution (Lonza) (HCT116-Cas9 ...
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bioRxiv - Molecular Biology 2023Quote: ... or Vero-hACE2-TMPRSS2 cells (Vero AT) (BEI NR-54970) in Eagle’s Minimum Essential Medium (Lonza, 12-125Q) supplemented with 2% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2023Quote: ... The neutrophil pellet was then cleared of remaining red blood cells using ACK lysis buffer (Lonza #10-548E). For subsequent experiments ...
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bioRxiv - Genetics 2023Quote: ... The cell suspension was transferred to the nucleofection cuvete and nucleofection was performed on the 4D-Nucleofector (Lonza) on the program for hESC ...
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bioRxiv - Immunology 2023Quote: ... 24-well plate) at a concentration of 75,000 cells per well in Smooth muscle growth medium (SmGM) (Lonza) and left to adhere and spread for 90 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... 6-well dishes were seeded with 1.0 × 106 Sf9 cells per well in 2.0 mL InsectExpress medium (Lonza). Cells were transfected with 20 mL of bacmid per well ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were additionally screened for their sterility and the absence of mycoplasma during this process (LT07-703, Lonza).
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bioRxiv - Cell Biology 2023Quote: ... The RNP complex was mixed with confluent 2i-grown ES cells re-suspended in P3 transfection buffer (Lonza) before being transferred to an electroporation microcuvette well (Lonza) ...
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bioRxiv - Cancer Biology 2023Quote: ... All cell lines were cultured less than 3 months after resuscitation and tested for contaminants using MycoAlert (Lonza) every 1-3 months to ensure they were free of Mycoplasma contamination.
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bioRxiv - Developmental Biology 2023Quote: Human skeletal muscle myoblasts (CC-2561) and subcutaneous preadipocyte cells (PT-5020) were purchased from Lonza (Lonza Walkersville). Subcutaneous preadipocyte cells were induced to differentiate into terminal white adipocytes according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2023Quote: ... with a blue fluorescent protein (BFP)-tag were cultured in smooth muscle cell growth medium-2 (SMGM2) (Lonza). Human umbilical cord vein endothelial cells (HUVEC ...
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bioRxiv - Biochemistry 2023Quote: ... Briefly, D.mel-2 cells (CRL-1963, ATCC) were grown at 25°C in Insect-Xpress medium (181562, Lonza) supplemented with 1% Pen/Strep (15140122 ...
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bioRxiv - Cancer Biology 2023Quote: ... The cells were resuspended in an equal volume of molten 1% InCert agarose (Lonza Rockland, Inc., Rockland, ME) in PBS ...
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bioRxiv - Bioengineering 2023Quote: ... were cultured using the EGM™-2 MV Microvascular Endothelial Cell Growth Medium BulletKit™ (Lonza, CC-3202), which contains hydrocortisone ...
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bioRxiv - Microbiology 2023Quote: ... Cell lines were confirmed to be mycoplasma-negative in a MycoAlert PLUS assay (Lonza catalog number LT07-710) performed by the Koch Institute High-Throughput Sciences Facility (Cambridge ...
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bioRxiv - Immunology 2023Quote: ... or KSQ-001 cells were thawed and rested in serum-free X-VIVO15 media (Lonza, Cat# 04-418Q) overnight ...
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bioRxiv - Genetics 2023Quote: ... 2−106 lymphoblastoid cells were suspended in 100 μL of Nucleofector C solution (VCA-1004, Lonza Cologne AG) with 0.6 μM RNAi and transfected with the Z-001 program according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: Human aortic endothelial cells (ATCC, Manassas, VA) were cultured in EGM-2 culture media (Lonza Walkersville, Basel, Switzerland) on 0.1% gelatin-coated plastic dishes until ∼80% confluence ...
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bioRxiv - Cell Biology 2023Quote: ... All cells were used at passage 1-2 and were growth arrested in smooth muscle basal media (Lonza) supplemented with 0.3% FBS for 24-48 h before beginning experiments ...
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bioRxiv - Bioengineering 2023Quote: ... The supernatant medium from cell cultures was analyzed monthly for the presence of mycoplasma using MycoAlert PLUS (Lonza) or via polymerase chain reaction (PCR).
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bioRxiv - Molecular Biology 2023Quote: ... The culture medium used was EGMTM-2 MV Microvascular Endothelial Cell Growth Medium-2 BulletKitTM (Lonza, Basel, Switzerland). The cells were cultured for 24 h and total RNA was collected as described in the RT-PCR protocol.
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bioRxiv - Bioengineering 2024Quote: ... Insect Drosophila melanogaster Schneider S2 cells were cultivated at 26°C in Insect-XPRESS media (Lonza, Basel, Switzerland) supplemented with 2 mM L-glutamine.
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bioRxiv - Microbiology 2024Quote: ... Human umbilical endothelial cells (HUVEC-C; ATCC, CRL-1730) were cultured in Lonza EGM-Plus (Lonza, CC-4542) supplemented with bullet kit (Lonza ...
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bioRxiv - Cancer Biology 2024Quote: Bone marrow KLS cells were sorted from TauT+/ or TauT-/- mice and cultured overnight in X-VIVO15 (Lonza) media supplemented with 10% fetal bovine serum (Gemini) ...
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bioRxiv - Immunology 2024Quote: ... The total number of cells prior to cryopreservation were counted diluted 1:1 in Trypan Blue dye (Lonza), using a haemocytometer ...
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bioRxiv - Molecular Biology 2024Quote: HUVECs were cultured in EGMTM-2 Endothelial Cell Growth Medium-2 BulletKitTM (Lonza: CC-3156 and CC-4176) for 24 hours before transfecting with RNAiMax (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2024Quote: ... Daudi cells were then washed with PBS twice and resuspended in SF solution (Lonza, catalog number V4XC-2032), 300k cells per 20 µl solution ...
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bioRxiv - Microbiology 2021Quote: ... electroporated with 10 μg of linearised plasmid DNA or PCR product using the Amaxa Basic Parasite Nucleofector Kit 1 and Nucleofector II device (Lonza, Switzerland, program X-001).
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bioRxiv - Cancer Biology 2021Quote: ... cells were also transfected with electroporation using Amaxa™ Nucleofector Kit R (Cat.no.VCA-100) and the Nucleofector™II device (program W-001, Lonza Group AG, Cologne, Germany) as suggested by the manufacturer.
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bioRxiv - Developmental Biology 2022Quote: Generation of Foxa2 knockout cell lines was performed by electroporating two different guides cloned into the pX458 vector (Ran et al., 2013) with the AMAXA nucleofector kit (Lonza Cat no. VPH-1001). Cells were sorted for GFP as single cells one day after electroporation ...
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bioRxiv - Cell Biology 2023Quote: ... The cell line was authenticated through short tandem repeat analysis and tested for mycoplasma contamination every 10 passages using the MycoAlert Mycoplasma Detection Kit (Lonza Group Ltd, Basel, Switzerland). PDGFR-BiFC and myr-Venus stable cells were cultured in RPMI growth medium [RPMI 1640 (Gibco ...
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bioRxiv - Bioengineering 2023Quote: The murine myoblast cell line C2C12 was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and was tested for mycoplasma (MycoAlert™ Mycoplasma Detection Kit, Lonza AG, Basel, Switzerland). Cells were cultured in monolayer at 37 °C in a 5% CO2-containing humidified atmosphere in complete growth medium (GM) ...
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bioRxiv - Synthetic Biology 2023Quote: ... CD22-deleted clones modified to express PD-L1 (LZRS PD-L1) or CD200 (PB-EF-1α-intron CD200, pCMV-hyPBase, SF buffer kit V4XC-2032, Lonza 4D Nucleofector, code DN100). Transposition reactions used 1-2 μg of vector DNA and 0.5-1.0 μg of pCMV-hyPBase transposase DNA ...
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bioRxiv - Genomics 2023Quote: ... All iPSCs were karyotypically validated via g-banded karyotyping (WiCell) and are regularly screened for mycoplasm via MycoAlert™ PLUS Mycoplasma Detection Kit (Lonza Cat # 75860-362). Two major approaches to generating neurons in a dish are based on overexpression of proneural factors combined with chemicals from iPSCs differentiation or directly converted from fibroblasts ...
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bioRxiv - Microbiology 2020Quote: ... Human neuroblastoma SH-SY5Y cells were grown in a 1:1 (v/v) mixture of EMEM with EBSS (Lonza) and Ham’s F12 (Gibco ...
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bioRxiv - Developmental Biology 2021Quote: HEK293T/17 cells (ATCC CRL-11268) were grown in Dulbecco’s modified Eagle medium (Hyclone) supplemented with 10% fetal bovine serum (Lonza) and penicillin-streptomycin (Gibco) ...
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bioRxiv - Developmental Biology 2020Quote: Transfection mixture was left on cells for 12-14 hours and viral production medium (Ultraculture, Lonza BioWhittaker 12-725F) supplemented with 1% Pen/Strep ...
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bioRxiv - Cancer Biology 2022Quote: ... To assay anoikis resistance and anchorage-independent proliferation IEC6 cells were grown in 0.35% low-melt agarose (Lonza SeaPlaque). A base layer of agarose was prepared by melting pre-sterilized 1.2% agarose in dH2O at 75°C ...
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bioRxiv - Biochemistry 2022Quote: ... S2 cells were selected at 27°C in complete Schneider’s medium and then transferred to Insect Xpress medium (Lonza) for large-scale expression in 2-liter Erlenmeyer flasks ...
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bioRxiv - Bioengineering 2022Quote: NK-92 cell line was acquired from the American Type Culture Collection and maintained in X-VIVO 10 (Lonza) supplemented with 20% FBS and 0.02 mM folic acid (Millipore Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were trypsinized and resuspended in a mixture 0.5% w/v Low molecular weight agarose (Lonza, cat. No. #50101) and PBS at a ratio of 10:1 ...