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Citations for Worthington Biochemical :
151 - 200 of 203 citations for Urothelial Cell Culture Supplements since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Niche cells from the bone marrow fraction were isolated by digestion with 1 mg/mL STEMxyme1 (Worthington, Ref#LS004106) and 1 mg/mL Dispase 1 (ThermoFisher Scientific ...
Product cited:
A specialized combination of Animal Free Clostridium histolyticum collagenase (Code: CLSAFB) and...Cat# LS004106, 50 mg, $110.00 Ask -
bioRxiv - Neuroscience 2022Quote: Individual brain organoids were dissociated into a single-cell suspension using the Worthington Papain Dissociation System kit (Worthington Biochemical). A detailed description of the dissociation protocol is available at Protocol Exchange ...
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bioRxiv - Neuroscience 2022Quote: Individual brain organoids were dissociated into a single-cell suspension using the Worthington Papain Dissociation System kit (Worthington Biochemical). A detailed description of the dissociation protocol is available at Protocol Exchange ...
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bioRxiv - Cancer Biology 2023Quote: ... 2×106 HCT116 cells were used per each immunoprecipitation (IP) reaction and 15 units of micrococcal nuclease (MNase, Worthington) was used to digest the chromatin for the ChIP reaction ...
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bioRxiv - Bioengineering 2023Quote: ... or in vivo transplantation using an adaptation of a well-established neural cell isolation method (Worthington 9035-81-1).71 As with our undifferentiated hiPSC cultures ...
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bioRxiv - Neuroscience 2023Quote: Cortex of E12.5 and E14.5 embryos was dissected and dissociated into single cells using the Papain Dissociation System (#LK0031500, Worthington Biochemical). Individual collected cells were processed for scRNAseq using ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Bioengineering 2023Quote: Cells were isolated from semitendinosus muscles of Belgian Blue cattle by collagenase digestion (CLSAFA, Worthington; 1 h; 37 °C), filtration with 100 μm cell strainers ...
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bioRxiv - Immunology 2023Quote: ... Cells were then DNase treated with 4 ml disaggregation media (DMEM, 10% FBS, 100 μg/ml DNASE I (Worthington)) for 20 min at 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells cultured on collagen and mineralized collagen were collected using collagenase type I (1mg/mL in PBS, Worthington Biochemical) and passed through a 40 µm cell strainer ...
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bioRxiv - Molecular Biology 2019Quote: ... of 0.1 focus-forming unit (FFU)/cell in M199 medium supplemented with 1 mg/mL porcine pancreatic trypsin (Worthington Biochemical). Cell culture medium was collected 24–36 h post infection ...
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bioRxiv - Cell Biology 2019Quote: ... A single cell suspension was made by digesting tissue for 30 minutes at 37°C in Collagenase type 2 solution (Worthington Biochemical ...
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bioRxiv - Neuroscience 2020Quote: ... NBS1- and NBS8-organoids were dissociated to a single cell suspension for 30 minutes using the Papain dissociation kit (Worthington) according to the manufactures’ instructions ...
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bioRxiv - Microbiology 2020Quote: ... All viruses were titrated using 1:10 serial dilution in triplicate in LLCMK2 cells in the presence of 2μg/mL TPCK trypsin (Worthington Biomedical) for 72 hours and TCID50/mL was determined by the Reed-Meunch method based on hemagglutination of 0.5% washed chicken red blood cells (Lampire ...
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bioRxiv - Cell Biology 2021Quote: ... The hypothalamus and pre-optic area were then dissected from vibratome sections and dissociated into single cells using the Papin Dissociation Kit (Worthington) as previously described24 ...
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bioRxiv - Bioengineering 2022Quote: ... cells showed spontaneous beating and were dissociated on day 15 by incubation with 280 U/ml Collagenase (LS004174, Worthington, USA) and 40 U/ml DNase (LS006331 ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004174, 100 mg, $42.00 Ask -
bioRxiv - Cell Biology 2020Quote: ... were generated using the eight plasmid-based systems as previously described (Wit et al., 2004) and propagated on MDCK-II cells in presence of TPCK-trypsin (Worthington). For infection ...
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bioRxiv - Microbiology 2019Quote: ... was propagated in MA104 cells in serum-free DMEM in the presence of 1 µg/mL Worthington’s Trypsin (Worthington Biochemical). After harvest ...
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bioRxiv - Cancer Biology 2020Quote: A total of ten tumor specimens were dissociated into a single cell suspension using a Papain Dissociation System (The Worthington Papain Dissociation System ...
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bioRxiv - Neuroscience 2023Quote: ... Individual cells were isolated by enzymatic digestion with 0.5 mg/ml neutral protease and 0.5 mg/ml elastase (Worthington Biochemical Corporation) in endothelial cell (EC ...
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bioRxiv - Developmental Biology 2023Quote: ... washed with PBS and dissociated into single cells using collagenase type 4 enzyme at 200Units/ml (Worthington, Lakewood, NJ, US) for 10min at 37°C ...
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bioRxiv - Immunology 2023Quote: T cells that infiltrate pancreatic islets were isolated after intra-bile duct injection and digestion with collagenase IV (Worthington Biochemical) 28 ...
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bioRxiv - Cancer Biology 2023Quote: ... and dissociated into single-cell suspensions with an enzymatic digestion buffer consisting of Collagenase-II (for subcutaneous tumors) (Cat: LS004176, Worthington) or Collagenase-III (for breast tumors ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Evolutionary Biology 2023Quote: ... Ganglia were cut into small pieces and cells were dissociated by incubating ganglia pieces in enzyme solution (10 mg/mL collagenase IV (Worthington), in 1XHBS (HEPES buffered saline ...
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bioRxiv - Developmental Biology 2023Quote: ... Cortical organoids were dissociated into single-cell suspensions via 30-min incubation at 37 °C in papain and DNAse in EBSS (Cat. # LK003150, Worthington), shaking vigorously every 5-10 min ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Cancer Biology 2024Quote: ... Cells were crosslinked with 1% formaldehyde and then 2.5 x 106 cells were treated with 60 U of MNase (Worthington, LS004797) and Exonuclease III (E ...
Product cited:
Chromatographically purified to be essentially homogeneous chromatographically and...Cat# LS004797, 15 ku, $94.00 Ask -
bioRxiv - Genetics 2024Quote: Testicular cell suspension was prepared by incubation of seminiferous tubules in 200 U/ml Collagenase type I (Worthington Biochemical LS004196), 5 μg/ml DNAse I (Roche 10104159001) ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004196, 1 gm, $215.00 Ask -
bioRxiv - Molecular Biology 2020Quote: Cortical cell suspension were obtained by dissociating cerebral cortices from E18 embryos using the Papain Dissociation System (Worthington, cat. no. LK003150). One million live cells were plated in 5 ml complete Neurobasal culture medium (Neurobasal™ Medium (Gibco ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Neuroscience 2021Quote: ... The SVZ was then dissociated into single cell suspension following the manufacturer’s protocol in the papain dissociation kit (Worthington Biochemicals # LK003150)41 ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Neuroscience 2020Quote: ... Cell mixture is centrifuged 6 minutes at 300 x g and after carefully aspirating the supernatant the cell pellet is then resuspended with EBSS plus ovomucoid inhibitor albumin mixture supplemented with DNase (each part of Worthington kit). Gentle trituration with a 10 ml pipette held several centimeters from the tube bottom is sufficient to resuspend ...
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bioRxiv - Neuroscience 2021Quote: Five independent samples of midbrain organoids (Table S1) were gently dissociated to single cells with papain enzymatic dissociation (Worthington Biochemical Corporation). After filtering cell clumps with a cell strainer (35um mesh) ...
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bioRxiv - Neuroscience 2022Quote: ... Each thalamus was cut into small pieces and dissociated into a single cell suspension using the Papain Dissociation System (Worthington, LK003153) according to the manufacturer’s protocol ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003153, 3 bx, $775.00 Ask -
bioRxiv - Neuroscience 2019Quote: Primary mouse hippocampal and cortical neurons were dissociated into single-cell suspensions from E16.5 mouse brains with a papain dissociation system (Worthington, Cat# LK003153). Neurons were seeded onto poly-L-lysine–coated plates (0.1% (wt/vol) ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003153, 3 bx, $775.00 Ask -
bioRxiv - Cancer Biology 2020Quote: Cells were derived from a minced piece of tumour digested in DMEM supplemented with 200U/ml collagenase type IV (Worthington, USA) at 37 °C for 24 h ...
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bioRxiv - Neuroscience 2023Quote: ... Pools of cortical organoids from each donor were enzymatically dissociated into a single-cell suspension using the Worthington Papain Dissociation System kit (Worthington Biochemical). Cell suspensions derived from different donors were mixed at equal ratios ...
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bioRxiv - Molecular Biology 2024Quote: ... nuclei from 2 X 108 Hela or 293T cells were digested with 20 U of micrococcal nuclease (Worthington Biochemical Corporation, LS004797) for 45 minutes ...
Product cited:
Chromatographically purified to be essentially homogeneous chromatographically and...Cat# LS004797, 15 ku, $94.00 Ask -
bioRxiv - Neuroscience 2020Quote: The organoids cultured from both 24w and 96w plates were dissociated into single cell suspension using a commercially available papain digestion kit (Worthington-biochem, LK003150). Live cells and dead cells from the dissociated organoids were stained with Calcium Green AM (ThermoFisher C3011MP ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Neuroscience 2019Quote: ... neural retinas were digested at 37°C for 9 minutes in papain solution and single cells were obtained following manufacturer instructions (Papain Dissociation System, Worthington Biochemical, USA). Living cells were counted using a hemocytometer after trypan blue staining and resuspended in PBS at a density of 1×105 cells/μl ...
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bioRxiv - Bioengineering 2021Quote: ... Muscles were minced to create a homogenous cell slurry and then incubated for 2 h at 37C in 0.2% collagenase (Worthington Biochemicals, Lakewood, NJ) in DMEM ...
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bioRxiv - Cell Biology 2020Quote: Lung samples from mice of different ages (P0-P62) were dissociated into a single cell suspension using the Papain Dissociation System (Worthington Biochemical Corporation) with a modified protocol ...
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bioRxiv - Genomics 2022Quote: ... Nuclei were collected by centrifugation and DNase I-treated for 30 min at 37°C (Worthington, 30U per 5 x 106 cells). After DNase I digestion ...
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bioRxiv - Developmental Biology 2023Quote: 30 hindbrains of st.18 chick embryos were harvested and disassociated into single cells using collagenase type 4 (200 Units\ml, Worthington 47B9407, USA). Cells were then centrifuged at 600 g for 10 min ...
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bioRxiv - Developmental Biology 2023Quote: ... 60 hindbrains of st.18 chick embryos were harvested and disassociated into single cells using collagenase type 4 (200 Units\ml, Worthington 47B9407, USA). Cells were then centrifuged at 600 g for 10 min ...
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bioRxiv - Neuroscience 2023Quote: The primary neuronal cells were isolated from the brain tissues of 1∼2-day-old SD pups using the papain dissociation system (Worthington Biochemical Corporation) as instructed by the manufacturer ...
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bioRxiv - Neuroscience 2023Quote: ... 4–5 randomly selected organoids were pooled to obtain single cell suspension from hDiO and then incubated in 30 U/mL papain enzyme solution (Worthington Biochemical, LS003126) and 0.4% DNase (12,500 U/mL ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003126, 100 mg, $88.00 Ask -
bioRxiv - Immunology 2023Quote: ... for 5 min at 4°C to remove circulating cells and then digested in RPMI 1640 with 1 mg/mL Type 2 collagenase (Worthington Biochemical LS004177) for 45 min at 37°C ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004177, 5 gm, $900.00 Ask -
bioRxiv - Molecular Biology 2023Quote: Uterine pieces were washed with phenol red-free free HBSS(−) to remove epithelial cells and further incubated in phenol red-free free HBSS(−) with collagenase type III (#CLS3, Worthington, Lakewood, NJ) at 37.5°C for 30 min ...
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bioRxiv - Neuroscience 2024Quote: ... 4–5 randomly selected organoids were pooled to obtain single cell suspension and then incubated in 30 U/mL papain enzyme solution (Worthington Biochemical, LS003126) and 0.4% DNase (12,500 U/mL ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003126, 100 mg, $88.00 Ask -
bioRxiv - Cell Biology 2021Quote: ... A single cell suspension of vascular stromal cells was isolated from minced iBAT (using scissors) digested for 90 min with collagenase IV (3 mg mL-1, Worthington, Lakewood NJ, USA) at 37 °C under gentle agitation16 ...
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bioRxiv - Cell Biology 2022Quote: Cells were isolated from semitendinosus muscles of 10 Belgian Blue cattle (Supplementary Table 1) by collagenase digestion (CLSAFA, Worthington; 1 h; 37 °C), filtration with 100 μm cell strainers ...
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bioRxiv - Genetics 2019Quote: Primary mouse cortical neurons were dissociated into single cell suspensions from E16.5 mouse (strain: C57BL/6J) cortices using a papain dissociation system (Worthington Biochemical Corporation, Lakewood, NJ). Neurons were seeded onto poly-L-lysine coated plates (0.1% w/v ...