Papain Dissociation System
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's balanced salt solution (EBSS), and an inhibitor vial for use in the tissue dissociation method of Huettner, J., and Baughman, R., J. Neuroscience, 6, 3044 (1986). Use-tested by Worthington using new-born rat pup spinal cord. The package contains sufficient materials for dissociation of five separate tissue aliquots of up to 0.3-0.4 cm3 each.
| Supplier | Worthington Biochemical |
|---|---|
| Product # | LK003153 |
| CAS # | see components |
| Pricing | 3 bx, $775.00 |
| Storage Conditions | Store at 2-8°C. |
| Product Code | PDS |
| UNSPSC Code(s)* | 12161503, 12352204 |
Citations for this product:
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bioRxiv - Neuroscience 2021Quote: ... Neurons were dissociated with the Papain Dissociation System (Worthington Biochemical Corporation, cat# LK003153). Cells were seeded on Poly-L-lysine (Sigma Aldrich ...
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bioRxiv - Immunology 2021Quote: ... mouse SC were minced into ∼1mm2 pieces and digested using the Papain Dissociation System (Worthington Biochemical Corporation, Cat# LK003153). Isolated cells were negatively selected tandemly first with Myelin Removal Beads II (Miltenyi Biotec ...
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bioRxiv - Developmental Biology 2021Quote: ... according to the manufacturer’s protocol (Worthington, cat. #LK003153). For nuclei preparation ...
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bioRxiv - Physiology 2022Quote: ... brain cortex tissues were dissected and digested using the enzyme-cocktail solution of 30U/ml papain (LK003153; Worthington) and 0.125mg/ml Liberase TM (5401119001 ...
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bioRxiv - Neuroscience 2022Quote: ... Each thalamus was cut into small pieces and dissociated into a single cell suspension using the Papain Dissociation System (Worthington, LK003153) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: Primary mouse hippocampal and cortical neurons were dissociated into single-cell suspensions from E16.5 mouse brains with a papain dissociation system (Worthington, Cat# LK003153). Neurons were seeded onto poly-L-lysine–coated plates (0.1% (wt/vol) ...
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bioRxiv - Developmental Biology 2024Quote: Organoids were dissociated using a previously described protocol53,78 which is a modification of the Worthington Papain Dissociation Kit manufacturer’s protocol (Worthington, #LK003153). Briefly ...
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bioRxiv - Genomics 2024Quote: ... Dissociation was performed using Papain and Deoxyribonuclease I (Worthington Biochemical Cat. no. LK003153) for 20 min at 37°C and stopped using Earl’s balanced salt solution (EBSS) ...
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bioRxiv - Genomics 2024Quote: Single cell dissociation was performed using Papain and Deoxyribonuclease I (Worthington Biochemical Cat. no. LK003153) for 20 min at 37°C and stopped using Earl’s balanced salt solution (EBSS-Thermo Fisher Scientific ...
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Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neuronsbioRxiv - Neuroscience 2024Quote: ... iNs were dissociated using the Papain Dissociation System following the manufacturer’s protocol (Worthington Biochemical Cat#: LK003153) and then transplanted onto HFA-covered coverslips with thiazovivin (1μg/mL ...