Papain Dissociation System
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's balanced salt solution (EBSS), and an inhibitor vial for use in the tissue dissociation method of Huettner, J., and Baughman, R., J. Neuroscience, 6, 3044 (1986). Use-tested by Worthington using new-born rat pup spinal cord. The package contains sufficient materials for dissociation of five separate tissue aliquots of up to 0.3-0.4 cm3 each.
| Supplier | Worthington Biochemical |
|---|---|
| Product # | LK003153 |
| CAS # | see components |
| Pricing | 3 bx, $775.00 |
| Storage Conditions | Store at 2-8°C. |
| Product Code | PDS |
| UNSPSC Code(s)* | 12161503, 12352204 |
Citations for this product:
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bioRxiv - Neuroscience 2021Quote: ... Neurons were dissociated with the Papain Dissociation System (Worthington Biochemical Corporation, cat# LK003153). Cells were seeded on Poly-L-lysine (Sigma Aldrich ...
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bioRxiv - Immunology 2021Quote: ... mouse SC were minced into ∼1mm2 pieces and digested using the Papain Dissociation System (Worthington Biochemical Corporation, Cat# LK003153). Isolated cells were negatively selected tandemly first with Myelin Removal Beads II (Miltenyi Biotec ...
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bioRxiv - Developmental Biology 2021Quote: ... according to the manufacturer’s protocol (Worthington, cat. #LK003153). For nuclei preparation ...
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bioRxiv - Physiology 2022Quote: ... brain cortex tissues were dissected and digested using the enzyme-cocktail solution of 30U/ml papain (LK003153; Worthington) and 0.125mg/ml Liberase TM (5401119001 ...
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bioRxiv - Neuroscience 2022Quote: ... Each thalamus was cut into small pieces and dissociated into a single cell suspension using the Papain Dissociation System (Worthington, LK003153) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: Primary mouse hippocampal and cortical neurons were dissociated into single-cell suspensions from E16.5 mouse brains with a papain dissociation system (Worthington, Cat# LK003153). Neurons were seeded onto poly-L-lysine–coated plates (0.1% (wt/vol) ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were dissociated from the brain using the papain dissociation system (Worthington LK003153). Cells were then resuspended in 500 μL of NPC culture media (NeuroCult™ Proliferation Media ...
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bioRxiv - Neuroscience 2023Quote: The lesioned grey matter of the somatosensory cortex of C57BL/6J mice at 3dpi and 5dpi or the corresponding region of the noninjured cortex were isolated using a biopsy punch (∅ 0.25cm) and the cortical cells were dissociated at a single cell level using the Papain Dissociation System (Worthington, # LK003153) followed by the Dead Cell Removal kit (Miltenyi Biotec # 130-090-101) ...
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bioRxiv - Neuroscience 2023Quote: Day 85 cultures from 16 lines were detached enzymatically in parallel using papain (Worthington; LK003153) and were filtered through 40µm Flowmi Cell Strainers (Scienceware ...
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Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neuronsbioRxiv - Neuroscience 2024Quote: ... iNs were dissociated using the Papain Dissociation System following the manufacturer’s protocol (Worthington Biochemical Cat#: LK003153) and then transplanted onto HFA-covered coverslips with thiazovivin (1μg/mL ...