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Citations for Worthington Biochemical :
1 - 50 of 208 citations for Primary Bovine Aortic Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2019Quote: ... aortic leaflets were placed in 2 mg/ml type II collagenase (Worthington Biochemical Corp.) in Advanced Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Pathology 2023Quote: ... Aortic tissue was cut into small rings and digested with 1 mg/ml collagenase type II (Worthington) and 0.5 mg/ml elastase (Worthington ...
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bioRxiv - Neuroscience 2020Quote: ... Individual capillary endothelial cells were isolated by enzymatic digestion with 0.5 mg/ml neutral protease (Worthington Biochemical Corp., USA) and 0.5 mg/ml elastase (Worthington ...
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bioRxiv - Neuroscience 2020Quote: ... glial cell line-derived neurotrophic factor (GDNF) and fetal bovine serum (FBS) were purchased from Worthington Biochemical Corporation (Lakewood ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were digested by adding bovine trypsin (Worthington-LS003750) and endoproteinase Lys-C (NEB-P8109S ...
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bioRxiv - Neuroscience 2020Quote: Primary human cortical tissue was dissociated with Papain (Worthington)-containing DNase ...
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bioRxiv - Biochemistry 2021Quote: ... Partially purified (DP grade) bovine pancreatic DNase I powder was purchased from Worthington Biochemical Corporation (#LS002139 ...
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bioRxiv - Neuroscience 2021Quote: ... Primary cultures of neurons were prepared using the Papain Dissociation System (Worthington) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... 0.2% Bovine Serum Albumin (BSA) (MP Biomedical) and 1 µg/mL TPCK-trypsin (Worthington Biomedical)) ...
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bioRxiv - Genomics 2020Quote: ... Processing buffer (5% fetal bovine serum in PBS) and DNAse I (100 µg/ml, Worthington LS006344) were then added and the samples rocked at 37°C for 5 minutes ...
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bioRxiv - Genomics 2023Quote: Processing buffer (5% fetal bovine serum in PBS) and DNase I (100 μg/mL, Worthington LS006344) were then added ...
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bioRxiv - Physiology 2023Quote: Primary hepatocytes were isolated from C57BL/6 mice by collagenase (Worthington Biochemical Corporation, LS004188) perfusion and maintained in William’s E Medium with primary hepatocyte maintenance supplements (Gibco #CM4000 ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
bioRxiv - Neuroscience 2021Quote: ... Primary cultures of neurons were prepared from cerebral cortices using the Papain Dissociation System (Worthington) according to the manufacturer’s instructions and were plated and cultured as described above for 5xFAD mouse neurons ...
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bioRxiv - Developmental Biology 2024Quote: ... the suspension was washed twice with phosphate-buffered saline (PBS) containing 0.02% Bovine Serum Albumin (BSA, Worthington, #LS000291) twice and passed through a 30 μm cell strainer (Miltenyi Biotec ...
Product cited:
Prepared by a method developed at Worthington. Some degradation products may be present. ≥90%...Cat# LS000291, 5x100 mg, $260.00 Ask -
bioRxiv - Neuroscience 2019Quote: ... pellets were resuspended in 3 mL of EBSS containing 0.1 % of ovomucoid protease inhibitor and 0.1 % bovine serum albumin (Worthington, LK003182) to quench papain ...
Product cited:
Ovomucoid protease inhibitor and bovine serum albumin which is 0.22 micron filtered and...Cat# LK003182, 1 vi, $86.00 Ask -
bioRxiv - Neuroscience 2019Quote: ... pellets were resuspended in 3 mL of EBSS containing 0.1 % of ovomucoid protease inhibitor and 0.1 % bovine serum albumin (Worthington, LK003182) to quench papain ...
Product cited:
Ovomucoid protease inhibitor and bovine serum albumin which is 0.22 micron filtered and...Cat# LK003182, 1 vi, $86.00 Ask -
bioRxiv - Developmental Biology 2020Quote: ... followed by quenching of the enzyme using 1 mg/ml ovomucoid/bovine serum albumin solution with 0.1 mg/ml DNase I (Worthington LK003150). Cells were passed through a 70 μm strainer (PluriSelect 43-10070-70 ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Microbiology 2021Quote: ... 0.1% Bovine Serum Albumin (BSA) (MP Biomedical) and 1 µg/ml of TPCK (tolylsulfonyl phenylalanyl chloromethyl ketone)-trypsin (Worthington Biomedical) (Weingartl et al. ...
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bioRxiv - Genomics 2022Quote: Primary rat cortical neurons were prepared from E18-19 embryos using a papain dissociation kit (Worthington Biochemical) following the manufacturer’s instruction ...
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bioRxiv - Microbiology 2022Quote: ... of 0.01 and maintained in MEM containing 0.3% bovine serum albumin (MEM/BSA) and 1 μg/mL of L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK)-trypsin (Worthington, Lakewood, NJ, USA). Supernatants were collected every 12 h and viral titers were measured using a plaque assay.
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bioRxiv - Cell Biology 2020Quote: Organoid cultures within 2-3 weeks of primary plating were dissociated with 1 U/ml neutral protease (Worthington, Cat LS02100) and 100 KU of DNase I in organoid media ...
Product cited:
Animal Free, Chromatographically purified. A lyophilized powder.Cat# LS02100, 10 mg, $84.00 Ask -
bioRxiv - Pharmacology and Toxicology 2024Quote: Primary calvarial osteoblasts were isolated from calvaria of 5-day-old neonates using collagenase type II (50 mg/ml, Worthington, LS004176)/dispase II (100 mg/ml ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Genomics 2022Quote: Primary cultures of neonatal rat ventricular cardiomyocytes (CM) were isolated as previously described using the neonatal cardiomyocyte isolation system (Worthington Biochemical Corporation) from 1-3 day old Fischer rat pups (Golan-Lagziel et al. ...
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Abl kinase deficiency promotes AKT pathway activation and prostate cancer progression and metastasisbioRxiv - Cancer Biology 2020Quote: ... GS689.Li cells from primary tumors were recovered by mincing with a sterile razor blade and digesting with 200 U/ml collagenase II (Worthington Biochemical Corporation) for 15 min at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... Primary myofibroblasts were disaggregated from the fresh surgical tissues using 300 units/g type II collagenase at 1mg/ml (Worthington Chemical, NJ, USA) in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Biochemistry 2023Quote: ... Mixed cortical primary neurons from 16 to 18 embryonic-day-old embryos were enzymatically dissociated using the papain dissociation system (Worthington Biochemical Corporation, Lakewood, NJ). The neuronal cultures were maintained in Neurobasal medium supplemented with 2% B27 supplement ...
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bioRxiv - Genomics 2023Quote: ... Cells were dissociated using the Papain cell dissociation kit (Worthington, LK003150). Chromium 3’ single-cell RNA-seq kit (10xGenomics ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Genetics 2020Quote: ... cells were lifted using Papain (Worthington) with DNase (Worthington ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were dissociated with papain (Worthington) in the presence of DNAse I ...
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bioRxiv - Neuroscience 2021Quote: Retinal cells were dissociated into single cells using a Papain dissociation kit (#LK003150, Worthington Biochemicals, Lakewood, NJ, USA), as described by us previously (Ahmed et al. ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Neuroscience 2019Quote: ... retinal cells were dissociated into single cell suspensions using a Papain dissociation kit (Worthington Biochemicals, New Jersey, USA). 125 × 103 retinal cells/well were cultured in chamber slides (Beckton-Dickinson ...
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bioRxiv - Developmental Biology 2021Quote: ... The dissected hypothalamuses were further divided into 2-4 pieces before cell dissociation for single cell isolation using Papain Dissociation System (cat no. LK003150, Worthington Biochemical Corporation ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Neuroscience 2023Quote: The lesioned grey matter of the somatosensory cortex of C57BL/6J mice at 3dpi and 5dpi or the corresponding region of the noninjured cortex were isolated using a biopsy punch (∅ 0.25cm) and the cortical cells were dissociated at a single cell level using the Papain Dissociation System (Worthington, # LK003153) followed by the Dead Cell Removal kit (Miltenyi Biotec # 130-090-101) ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003153, 3 bx, $775.00 Ask -
bioRxiv - Genomics 2023Quote: ... Cells were then filtered through a 70µm cell strainer into an inactivation solution containing albumin-ovomucoid inhibitor (Worthington, Cat#LK003182), DNAseI (1mg/mL ...
Product cited:
Ovomucoid protease inhibitor and bovine serum albumin which is 0.22 micron filtered and...Cat# LK003182, 1 vi, $86.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... we collected single cells using papain (Worthington LS003126) digestion and prepared cells for scRNAseq analysis ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003126, 100 mg, $88.00 Ask -
bioRxiv - Developmental Biology 2024Quote: ... Once free from Matrigel the cells were digested into a single-cell suspension using TrypLE and DNAse I (Worthington Biochemical Corporation). Cells were filtered through a 20% Percoll (Sigma ...
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bioRxiv - Neuroscience 2023Quote: Whole organoids were dissociated to the single cell level in preparation for single-cell RNA sequencing using the Papain Worthington Kit (Worthington, LK003150). All solutions included in the kit were prepared according to the manufacturer’s instructions ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Neuroscience 2021Quote: ... cells were dissociated using Papain Dissociation System (LK003150, Worthington). Dissociated cells were resuspended in a buffer containing 9.8 ml Hibernate A ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Cell Biology 2020Quote: ... Cells were then dissociated with papain (Worthington, Cat# LK003178) supplemented with DNase (Worthington ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003178, 5 vi, $98.00 Ask -
bioRxiv - Cell Biology 2021Quote: ... and cells were dissociated by papain (Worthington, Cat# LS003127) digestion for 20 mins at 37°C ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003127, 1 gm, $595.00 Ask -
bioRxiv - Genomics 2019Quote: ... cells were treated with DNaseI (Worthington Chemical Corporation #NC9924263), triturated through increasingly narrow fire-polished pipettes ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were dissociated using Papain Dissociation System (#LK003150, Worthington) following manufacturer’s instructions ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
Increased ACTL6A Occupancy Within mSWI/SNF Chromatin Remodelers Drives Human Squamous Cell CarcinomabioRxiv - Molecular Biology 2021Quote: ... cells were pretreated with 200 U/ml DNase (Worthington) for 30 min at 37 °C to remove free-floating DNA and DNA from dead cells ...
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bioRxiv - Neuroscience 2023Quote: ... cells were incubated with DNase I (Worthington Biochemical Corporation) for 10 min at 37 °C ...
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bioRxiv - Neuroscience 2024Quote: ... and the cell suspension was digested in collagenase (Worthington) and neutral protease (Worthington ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were dissociated with prewarmed papain (Cat.#LK003176, Worthington) with DNAse (Cat.#LS006333 ...
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bioRxiv - Neuroscience 2021Quote: Cells were singularized for scRNAseq by dissociation with Papain (Worthington). Briefly ...
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bioRxiv - Neuroscience 2020Quote: ... and cells were dissociated using Papain Dissociation System (LK003150, Worthington). Briefly ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Neuroscience 2021Quote: ... Cells were resuspended with ovomucoid protease inhibitor (Worthington Biochemical Corporation) and 50 mg/ml DNase I in O2:CO2 equilibrated EBSS and passed through a 70 micron cell strainer ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were triturated and added to an equal ovomucoid (Worthington) volume then spun down at 300 g at 4 °C to pellet ...