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Citations for Worthington Biochemical :
1 - 50 of 142 citations for Medium Kit without Serum and with CultureBoost R since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... A saturated overnight LB culture was diluted 1:50 into 3mL of minimal medium with or without 0.2g/L cellulase (Worthington, purified exo- and endo-glucanases ...
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bioRxiv - Developmental Biology 2019Quote: Isolated mouse embryos were minced and digested in sorting medium (Ham’s F10 with 10% horse serum) containing 400U/ml Collagenase II (Worthington; LS004177) for 1h at 37 °C in a shaking water bath ...
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bioRxiv - Microbiology 2019Quote: ... after which the inoculum was replaced with 500 μl of serum-free medium with 1 mg/mL TPCK-treated trypsin (Worthington Biochemical Corporation). Samples were titered by tissue culture infectious dose 50 (TCID50 ...
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bioRxiv - Biochemistry 2023Quote: ... and 140 μL diH2O with or without 0.07 mg/mL a-chymotrypsin (Worthington Biochemical, Catalog #LS001432). Melanization activity was determined through SpectraMax iD5 spectrophotometer readings at 492 nm 30°C for 45 minutes with 1 reading/minute [53].
Product cited:
Three times crystallized and treated with 1-chloro-3-tosylamido-7-amino-2-heptanone (TLCK) to...Cat# LS001432, 100 mg, $75.00 Ask -
bioRxiv - Neuroscience 2023Quote: Cerebral cortices from P4-6 CD1 mouse pups were dissected in ice-cold HBSS without Mg2+ and Ca2+ and triturated with 2 ml of digestion buffer containing 20 U/ml papain (Worthington) and 100 U/ml DNase I (Worthington ...
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bioRxiv - Pathology 2022Quote: ... cleaned of all fat and digested for 10 min at 37°C in digestion medium (i.e. isolation medium supplemented with 1.5 mg/mL of collagenase type 2 [#LS004176, Worthington-Biochem] and 0.7 U/mL of elastase type III [#E0127 ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Microbiology 2019Quote: ... Growth in broth culture was performed in BHI (Becton, Dickinson, and Co., Sparks, MD) with and without catalase (5000 units, Worthington Biochemical Corporation, Lakewood, NJ) for aerobic cultivation and with Oxyrase® (Oxyrase ...
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bioRxiv - Genomics 2021Quote: ... Day 10 CRISPRi neurons cultured in the +AO medium and -AO medium were dissociated using Papain (Worthington; Code: PAP2; Cat. No.LK003178) and stained by CellRox Green (Invitrogen/Thermo Fisher Scientific ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003178, 5 vi, $98.00 Ask -
bioRxiv - Immunology 2020Quote: ... Digestion medium consisted of 30 IU/ml collagenase IV (Worthington), 1% FCS and 12.5µg/ml DNAse (Roche ...
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bioRxiv - Cancer Biology 2019Quote: ... mechanically disrupted and digested in serum free media containing 2mg/ml collagenase type I (Worthington) and DNase I (Sigma ...
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bioRxiv - Bioengineering 2024Quote: ... 0.2% Bovine Serum Albumin (BSA) (MP Biomedical) and 1 µg/mL TPCK-trypsin (Worthington Biomedical)) ...
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bioRxiv - Neuroscience 2020Quote: ... glial cell line-derived neurotrophic factor (GDNF) and fetal bovine serum (FBS) were purchased from Worthington Biochemical Corporation (Lakewood ...
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bioRxiv - Genomics 2020Quote: ... Processing buffer (5% fetal bovine serum in PBS) and DNAse I (100 µg/ml, Worthington LS006344) were then added and the samples rocked at 37°C for 5 minutes ...
Product cited:
Molecular Biology Grade. Chromatographically purified to remove RNase and protease. Supplied...Cat# LS006344, 500 un, $90.00 Ask -
bioRxiv - Genomics 2023Quote: Processing buffer (5% fetal bovine serum in PBS) and DNase I (100 μg/mL, Worthington LS006344) were then added ...
Product cited:
Molecular Biology Grade. Chromatographically purified to remove RNase and protease. Supplied...Cat# LS006344, 500 un, $90.00 Ask -
bioRxiv - Neuroscience 2021Quote: ... cells were incubated for 5 minutes at RT in fetal calf serum complemented with DNase I (Worthington), followed by mechanical dissociation ...
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bioRxiv - Immunology 2023Quote: ... minced tissues were resuspended in GKN + 10% fetal calf serum (FCS) with collagenase IV (Worthington Biochemical Corporation) and DNase (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: ... Aortas were digested in DMEM medium supplemented with 500U/ml Collagenase II (Worthington). After extensive washing cells were resuspended in PBS containing 10%FCS and 2mM EDTA kept on ice ...
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bioRxiv - Developmental Biology 2024Quote: ... the suspension was washed twice with phosphate-buffered saline (PBS) containing 0.02% Bovine Serum Albumin (BSA, Worthington, #LS000291) twice and passed through a 30 μm cell strainer (Miltenyi Biotec ...
Product cited:
Prepared by a method developed at Worthington. Some degradation products may be present. ≥90%...Cat# LS000291, 5x100 mg, $260.00 Ask -
bioRxiv - Microbiology 2020Quote: ... All four cell lines were infected with SARS-CoV-2/Australia/VIC01/2020 at a multiplicity of infection of 0.1 with infection inoculum composed of serum-free culture media and TPCK-treated trypsin (Worthington). The plates were incubated at 37 °C for 30 minutes ...
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bioRxiv - Neuroscience 2019Quote: ... pellets were resuspended in 3 mL of EBSS containing 0.1 % of ovomucoid protease inhibitor and 0.1 % bovine serum albumin (Worthington, LK003182) to quench papain ...
Product cited:
Ovomucoid protease inhibitor and bovine serum albumin which is 0.22 micron filtered and...Cat# LK003182, 1 vi, $86.00 Ask -
bioRxiv - Neuroscience 2019Quote: ... pellets were resuspended in 3 mL of EBSS containing 0.1 % of ovomucoid protease inhibitor and 0.1 % bovine serum albumin (Worthington, LK003182) to quench papain ...
Product cited:
Ovomucoid protease inhibitor and bovine serum albumin which is 0.22 micron filtered and...Cat# LK003182, 1 vi, $86.00 Ask -
bioRxiv - Immunology 2020Quote: ... minced tissue was resuspended in 10ml GKN + 10% fetal calf serum (FCS; Serana) with collagenase IV (Worthington Biochemical Corp.) and DNase (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... pH 7.2) and digested 30□min in digestion medium containing 20□U/mL papain (Worthington). Samples were then washed with plating medium (MEM ...
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bioRxiv - Neuroscience 2020Quote: ... Digestive medium was freshly prepared by dissolving 12 mg papain powder (Worthington, cat. no. LS003119) in 6 ml hibernate without calcium (BrainBits ...
Product cited:
Supplied as a lyophilized powder prepared from a 2X crystalline suspension, Code: PAP. To...Cat# LS003119, 100 mg, $100.00 Ask -
bioRxiv - Bioengineering 2023Quote: ... and enzymatically digested in washing medium with 0.3% (w/v) collagenase type II (Worthington Biochemical), 0.2% pronase (Sigma) ...
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bioRxiv - Developmental Biology 2020Quote: ... followed by quenching of the enzyme using 1 mg/ml ovomucoid/bovine serum albumin solution with 0.1 mg/ml DNase I (Worthington LK003150). Cells were passed through a 70 μm strainer (PluriSelect 43-10070-70 ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Cancer Biology 2020Quote: Spleens were collected into RPMI 1640 containing 2% fetal calf serum (FCS) and dissociated with a scalpel and type 3 collagenase (Worthington) and DNAse I ...
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bioRxiv - Microbiology 2021Quote: ... 0.1% Bovine Serum Albumin (BSA) (MP Biomedical) and 1 µg/ml of TPCK (tolylsulfonyl phenylalanyl chloromethyl ketone)-trypsin (Worthington Biomedical) (Weingartl et al. ...
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bioRxiv - Microbiology 2020Quote: ... 1 hour after infection media was changed to serum-free media supplemented with 1µg/ml tosyl phenylalanyl chloromethyl ketone (TPCK)-treated trypsin (Worthington-Biochemical). Passage experiments were performed by infecting cells at an MOI of 0.01 ...
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bioRxiv - Microbiology 2019Quote: ... was propagated in MA104 cells in serum-free DMEM in the presence of 1 µg/mL Worthington’s Trypsin (Worthington Biochemical). After harvest ...
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bioRxiv - Microbiology 2022Quote: ... then digested in 1X HBSS medium containing 1 mg/mL DNase I (Worthington Biochem.com, cat#LS002145) and 2.5 mg/mL Collagenase Type I (Worthington Biochem.com ...
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bioRxiv - Cell Biology 2022Quote: ... Tissue was then incubated with F12K medium containing 2 mg/ml collagenase type IV (Worthington LS004188) at 37°C with pipetting every 5-10 min until most of the glandular cells were released and appeared in solution as clusters ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
bioRxiv - Developmental Biology 2019Quote: ... Papain from Worthington kit (Worthington Biochemical Corporation, LK003150) was added and cortices were placed on a rotator at 37 °C for 30 minutes (E11.5 ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Cell Biology 2022Quote: ... The isolated IVD tissue was digested in medium containing 0.2% type 2 collagenase (Worthington Biochemical, Lakewood, NK) and 0.3% pronase (Roche ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumors were finely minced and digested in L-15 medium with 800 units/ml collagenase IV (Worthington) and 0.1 mg/ml DNase I (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... and physically dissociated followed by enzymatic digestion with papain (1:100 diluted in L15 medium; LS003126, Worthington) and 0.2 mg/mL DNase I (10104159001 ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003126, 100 mg, $88.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... Dissociation medium was aspirated until 1 mL remained and an activated papain solution (1:1 papain [Worthington-Biochem] with 13.6 mM Cysteine-HCL ...
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bioRxiv - Genomics 2024Quote: ... followed by resuspension in 30 ml digestion medium supplemented with collagenase II (1.2 mg/ml, Worthington, #LS004177). Cartilage was digested for 18 hours at 37°C with gentle shaking and filtered through a 70 µm strainer ...
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bioRxiv - Cell Biology 2021Quote: ... BM was flushed and bones were cut into small pieces that were digested in serum free αMEM containing 2mg/ml Collagenase type II (Worthington, Lakewood, NJ) for 2 hours at 37°C in a shaking water bath ...
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bioRxiv - Immunology 2021Quote: ... lungs were minced with a scalpel and resuspended in 10ml GKN + 10% fetal calf serum (FCS) with collagenase IV (Worthington Biochemical Corp.) and DNase (Sigma-Aldrich ...
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A Bidirectional Switch in the Shank3 Phosphorylation State Biases Synapses toward Up or Down ScalingbioRxiv - Neuroscience 2021Quote: ... 0.5 mM EDTA (Worthington Biochem kit), and 0.01% DNase (Sigma-Aldrich ...
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bioRxiv - Pathology 2023Quote: ... followed by a 15 min perfusion with William’s medium E containing 10 mM Hepes and 0.03% collagenase type 1 (125 U/mg; Worthington). Livers were further minced ...
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bioRxiv - Microbiology 2022Quote: ... of 0.01 and maintained in MEM containing 0.3% bovine serum albumin (MEM/BSA) and 1 μg/mL of L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK)-trypsin (Worthington, Lakewood, NJ, USA). Supernatants were collected every 12 h and viral titers were measured using a plaque assay.
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bioRxiv - Microbiology 2022Quote: ... with 10% fetal calf serum and antibiotics/antimycotics supplemented with 1g/ml tosyl phenylalanyl chloromethyl ketone (TPCK)-trypsin (Worthington Biochemical Corp., Lakewood, NJ).
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bioRxiv - Developmental Biology 2021Quote: ... day-28 chondrogenic pellets were rinsed and placed in an equal volume (1 pellet per 1 mL) of digestion medium (0.4% w/v type II collagenase [cat. num. LS00417; Worthington Biochemical ...
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bioRxiv - Immunology 2021Quote: ... Organs were minced and then digested at 37 °C in IMDM medium containing 2.0 mg/ml of type IV collagenase (Worthington), 0.125 mg/ml DNase I (Sigma ...
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bioRxiv - Cancer Biology 2021Quote: ... mechanically dissociated and transferred to basal medium containing 0.1% collagenase Type IV and 150 mg/ml DNase I (Worthington), and incubated for 30 min shaking at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... The digestion was stopped by the addition of a solution of trypsin inhibitors (Leibovitz’s L15 medium, 10% 1 mg ml−1 trypsin inhibitor (Worthington) and 2% 10 mg ml−1 DNAse) ...
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bioRxiv - Cancer Biology 2021Quote: Excised tumors were minced with scissors and enzymatically digested in RPMI medium containing 2.1 mg/ml collagenase type 1 (Worthington), 75 µg/ml DNase I (Worthington) ...
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bioRxiv - Neuroscience 2020Quote: ... and lysed with freeze/thawing in hypotonic buffer containing Dulbecco’s Modified Eagle Medium (DMEM, Welgene) and deoxyribonuclease I (DNase I, Worthington). After removing cell debris through centrifugation ...