Chymotrypsin, Alpha, TLCK Treated
Three times crystallized and treated with 1-chloro-3-tosylamido-7-amino-2-heptanone (TLCK) to inhibit trypsin activity (Shaw, et al., Biochemistry, 4, 2219, 1965). Dialyzed against 1 mM HCl to remove autolysis products and low molecular weight contaminants. Supplied as a dialyzed, lyophilized powder.
| Supplier | Worthington Biochemical |
|---|---|
| Product # | LS001432 |
| CAS # | 9004.07.3 |
| Pricing | 100 mg, $75.00 |
| Product Code | CDTLCK |
| Activity/Specification | ≥45 units per mg protein |
| UNSPSC Code(s)* | 12352204.0 |
| Source | Bovine Pancreas |
| Storage Conditions | Store at 2-8°C. |
Citations for this product:
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bioRxiv - Biochemistry 2023Quote: ... and 140 μL diH2O with or without 0.07 mg/mL a-chymotrypsin (Worthington Biochemical, Catalog #LS001432). Melanization activity was determined through SpectraMax iD5 spectrophotometer readings at 492 nm 30°C for 45 minutes with 1 reading/minute [53].