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Citations for Worthington Biochemical :
1 - 50 of 147 citations for Dig 6C SAH 1a b c since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: Mouse back skin and wound beds were dissected and digested into single cells using 1:100 collagenase 1A (Worthington) and Liberase TM for macrophage subsets ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were incubated with 1 mg/ml collagenase B (Worthington Biochemical) for 1 hour at 37°C ...
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bioRxiv - Physiology 2021Quote: ... beating cardiomyocytes were detached from plates with 1 mg/ml collagenase B (Worthington Biochemical), dissociated with 0.25% Trypsin/EDTA (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... samples were digested with 1:50 (m/m) lysyl endopeptidase C (Fujifilm) at 42 °C for 2 hours and 1:50 (m/m) trypsin (Worthington) at 37 °C overnight ...
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bioRxiv - Systems Biology 2020Quote: ... The dissociation was carried out in dissection buffer containing collagenase B (1-2 mg/ml) and hyaluronidase (1.2-2 mg/ml, Worthington Biochemical) at 37 °C with frequent agitation for 30 min ...
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bioRxiv - Biochemistry 2024Quote: ... epidydimal fat from 4-month-old male DIO C57BL/6J mice was dissected out and dissociated using 2 mg/ml Collagenase B (Worthington, CLSAFB) and 1 mg/ml soybean trypsin inhibitor (Worthington ...
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bioRxiv - Genetics 2023Quote: ... To digest the polypeptide 10 µL of Glu C enzyme (Worthington) at 100 µg/mL was added and the samples digested overnight at 37 °C ...
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bioRxiv - Cell Biology 2020Quote: ... at 4°C overnight and then digested with collagenase type II (Worthington). The cardiomyocyte fraction was collected after differential plating for 70 min at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... muscle fibers were digested with collagenase (CLSAFA, Worthington; 1 h, 37 °C), followed by 100 μm and 40 μm cell filtration steps ...
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bioRxiv - Physiology 2022Quote: ... at 37 °C with 229 U/ml collagenase type II (Worthington Biochemical Corporation), and washed in Tyrode solution for enzyme removal ...
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bioRxiv - Cell Biology 2020Quote: ... after digestion by papain and DNase (Worthington for 10 min at 37°C). Transfections were performed using the Amaxa nucleofector system following the manufacturer’s manual ...
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bioRxiv - Immunology 2021Quote: ... Kidneys were digested at 37°C in 1 mg/ml collagenase IV (Worthington) in complete RPMI for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... after digestion by papain and DNase (Worthington for 10 min at 37°C). Transfections were performed using the Amaxa nucleofector system following the manufacturer’s manual ...
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bioRxiv - Neuroscience 2020Quote: ... retinae were dissociated at 37°C for 30min with papain (LK003160, Worthington, Lakewood, NJ). The same volume of Neurobasal medium (Thermo Fischer ...
Product cited:
Complete kit as described for product Code: PDS, but without the Earle's Balanced Salt Solution (EBSS).Cat# LK003160, 1 bx, $265.00 Ask -
bioRxiv - Cell Biology 2021Quote: ... and digested for 16 hr at 37°C with sequencing grade trypsin (Worthington Biochemical) in a 1:100 trypsin to protein ratio ...
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bioRxiv - Cell Biology 2020Quote: Tissues were digested enzymatically for 30 minutes at 37°C with papain (Worthington, LS003126) 20 units/mL in Ames ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003126, 100 mg, $88.00 Ask -
bioRxiv - Neuroscience 2022Quote: ... Samples were then incubated at 37°C with OPC papain solution (Worthington Cat#LS003124) for 20 minutes with constant inversion to prevent tissue aggregation ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003124, 25 mg, $40.00 Ask -
bioRxiv - Neuroscience 2020Quote: ... and enzymatically digested at 37 °C first using 1 mg/ml Collagenase IV (Worthington, US) in L15 for 25 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... and digested overnight at 37 °C with 1 µg of trypsin (Worthington, LS003740 TPCK-treated). Samples were then acidified with formic acid (1 % final concentration).
Product cited:
A chromatographically purified, diafiltered, lyophilized powder that has been treated with...Cat# LS003740, 100 mg, $112.00 Ask -
bioRxiv - Pharmacology and Toxicology 2024Quote: ... then by collagenase type II for ∼16 hours at 37°C (583 Units/ml Worthington Biochemical Corp. ...
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bioRxiv - Cell Biology 2022Quote: ... followed by collagenase type II for ∼16 hours at 37°C (583 Units/ml Worthington Biochemical Corp. ...
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bioRxiv - Biochemistry 2023Quote: ... the particles were incubated at 37°C with 1 μg/μL trypsin TPCK (Worthington-Biochem) for 2 h to induce the proteolysis of BSA and measure in the spectrofluorometer ...
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bioRxiv - Immunology 2021Quote: ... tissues were digested in Collagenase solution for 1 hour at 37°C (Worthington Biochemical, Lakewood, NJ). Cells were washed once and blocked by Fc Block (Clone ...
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The cellular basis of protease activated receptor type 2 (PAR2) evoked mechanical and affective painbioRxiv - Neuroscience 2020Quote: ... Ganglia were incubated at 37□C for 25 minutes in 1 mg/ml papain (LS003119; Worthington), followed by 25 minutes of incubation at 37□C in 3 mg/ml collagenase type 2 (LS004176 ...
Product cited:
Supplied as a lyophilized powder prepared from a 2X crystalline suspension, Code: PAP. To...Cat# LS003119, 100 mg, $100.00 Ask -
bioRxiv - Molecular Biology 2024Quote: ... and incubated for 30 min at 37 °C in papain solution (10 units/mL, Worthington, LS003126) containing N-acetyl-L- cysteine (160 μg/ml ...
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Spatial 3D genome organization controls the activity of bivalent chromatin during human neurogenesisbioRxiv - Neuroscience 2024Quote: ... Tissue pieces were then placed in a pre-warmed (at 37 °C) solution of papain (Worthington) and incubated at 37 °C ...
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bioRxiv - Neuroscience 2024Quote: ... finely chopped and incubated for 30 min at 37°C with 1 mg/ml papain (Worthington), 1 mM CaCl2 ...
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bioRxiv - Developmental Biology 2024Quote: ... dissection products were then transferred in 250µl of 37°C pre-heated papain/L15 solution (Worthington, REF ...
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bioRxiv - Neuroscience 2021Quote: ... was added to halt the proteolysis and samples were centrifuged at 300xG for 10 minutes at 4°C and resuspended in a 1:1:10 solution of DNase:ovomucoid:Neurobasal media (Worthington:Worthington:Gibco). Cell suspensions were incubated with primary antibodies to CD11b and CD45 (table 1 ...
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bioRxiv - Neuroscience 2022Quote: ... and the cells dissociated for 30 min at 37 °C with 0.2% collagenase type III (Worthington LS004182) in Hibernate-A medium (Thermo A1247501) ...
Product cited:
Lower in secondary proteolytic contaminant activities but with typical collagenase activity. ...Cat# LS004182, 1 gm, $215.00 Ask -
bioRxiv - Immunology 2022Quote: ... Chopped samples were incubated at 37°C for 30 min in collagenase III (3 mg/ml; Worthington). Liver samples were excised and meshed into single-cell suspensions through 70 μm meshes ...
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bioRxiv - Physiology 2021Quote: ... followed by a 15-min incubation at 37°C in type II collagenase (1.0 mg/ml) (Worthington) in Mg-PSS ...
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bioRxiv - Immunology 2020Quote: Endoproteinase Gluc-C (Staphyloccoccus aureus Protease V8) and chymotrypsin were obtained from (Worthington Biochemical Corp., Lakewood, USA). The ultrapure milli-Q deionized water (MQ ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were pelleted 200g 5min at 4°C and resuspended in 200μl aCSF with 2.5% DNaseI (Worthington Papain system ...
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bioRxiv - Neuroscience 2022Quote: ... Tissue was then transferred to 1 mL tubes containing papain solution pre-warmed to 37° C (Worthington Biochemical LS003119 ...
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bioRxiv - Cancer Biology 2023Quote: ... and digested at 37°C for 30 min with 1 mg/mL Collagenase I (Worthington Biochemical, LS004194), 3 mg/mL Dispase II (Roche ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004194, 100 mg, $42.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... Cells were pelted 200g 5min at 4°C and resuspended in 200μl aCSF with 2.5% DnaseI (Worthington Papain system ...
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bioRxiv - Immunology 2024Quote: ... Spleen and lymph nodes were digested for 25 min at 37 °C with Collagenase IV (Worthington biochemical) and DNase I (Roche Diagnostics) ...
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bioRxiv - Neuroscience 2021Quote: ... was added to halt the proteolysis and samples were centrifuged at 300xG for 10 minutes at 4°C and resuspended in a 1:1:10 solution of DNase:ovomucoid:Neurobasal media (Worthington:Worthington:Gibco). Cell suspensions were incubated with primary antibodies to CD11b and CD45 (table 1 ...
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bioRxiv - Neuroscience 2020Quote: ... hippocampi were later incubated twice for 15 minutes at 37°C with 100 units of papain (Worthington, NY) in DM and rinsed three times in DM and subsequently three times in plating medium (MEM ...
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bioRxiv - Cancer Biology 2020Quote: ... and digested rotating for 30 minutes at 37°C with 1 mg/mL Collagenase I (Worthington Biochemical LS004194), 3mg/mL Dispase II (Roche 04942078001) ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004194, 100 mg, $42.00 Ask -
The cellular basis of protease activated receptor type 2 (PAR2) evoked mechanical and affective painbioRxiv - Neuroscience 2020Quote: ... followed by 25 minutes of incubation at 37□C in 3 mg/ml collagenase type 2 (LS004176; Worthington) and 2 mg/ml Dispase II (04942078001 ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Developmental Biology 2020Quote: ... urogenital ridges or adrenal glands were enzymatically dissociated at 37°C using the Papain dissociation system (Worthington #LK003150). Cells were resuspended in DMEM 2%FBS ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Physiology 2020Quote: ... and the isolated ventricles were digested in 37 °C KHB supplemented with 1g/100mL collagenase type-II (Worthington) and 600 μl of 2.5% Trypsin/100mL (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... the subcutaneous inguinal fat pad was isolated bilaterally and digested at 37°C for 1-1.5 hours in 0.2 % collagenase type I (Worthington). Murine ASCs were isolated from digested fat ...
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bioRxiv - Pathology 2022Quote: ... cleaned of all fat and digested for 10 min at 37°C in digestion medium (i.e. isolation medium supplemented with 1.5 mg/mL of collagenase type 2 [#LS004176, Worthington-Biochem] and 0.7 U/mL of elastase type III [#E0127 ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Developmental Biology 2023Quote: ... organoids were cut into smaller pieces with a scalpel and incubated at 37°C in papain solution (Worthington) for 30 minutes (Velasco et al. ...
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bioRxiv - Cell Biology 2024Quote: ... digested for 30 min at 37°C in 2 mg/mL collagenase II (Worthington Biochemical, Lakewood, NJ, USA) in DMEM containing 100 U/mL penicillin and 100 µg/mL streptomycin (GibcoTM ...
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bioRxiv - Physiology 2021Quote: ... The proteins were digested for 5 hours with Lys-C (Wako) at room temperature and overnight with trypsin (Worthington). Samples were acidified and diluted with TFA to a final concentration of 1% TFA before being loaded on the Sep-Pak Light C18 cartridges (Waters) ...
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bioRxiv - Immunology 2021Quote: ... Organs were minced and then digested at 37 °C in IMDM medium containing 2.0 mg/ml of type IV collagenase (Worthington), 0.125 mg/ml DNase I (Sigma ...