Labshake search
Citations for Worthington Biochemical :
301 - 350 of 450 citations for 7 Chlorothieno 2 3 c pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2021Quote: ... followed by 2 h digestion with 300 U ml-1 type I collagenase (Worthington Biochemical). Dissociated cells were seeded in culture plates ...
-
bioRxiv - Developmental Biology 2022Quote: ... were incubated in 2 mg/ml collagenase type II (260 U/mg, Worthington, Lakewood, NJ) in Dulbecco’s Modified Eagle’s Medium (DMEM ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... The perfusate was then switched to Tyrode’s solution containing collagen type 2 (Worthington, Columbus, OH) and protease type 14 (Sigma-Aldrich ...
-
bioRxiv - Developmental Biology 2021Quote: ... 200 μL of 20 mg/mL of type 2 collagenase in L15 (Cat.No. NC9870009; Worthington) were added and incubated on an orbital rotator at 28°C for 35 min ...
-
bioRxiv - Immunology 2020Quote: To prepare single-cell suspensions lungs were finely chopped with scissors in a 48 well plate and treated with 3 mg ml-1 collagenase III (Worthington, Lakewood, NJ), 5 μg ml-1 DNAse ...
-
bioRxiv - Neuroscience 2023Quote: ... Cell pellet was collected by centrifuged at 300 g for 5 min and then resuspended in 3 mL Ovomucoid protease inhibitor with FBS (Inhibitor-BSA Vial [LK003182], Worthington, Lakewood, NJ). To remove the debris ...
Product cited:
Ovomucoid protease inhibitor and bovine serum albumin which is 0.22 micron filtered and...Cat# LK003182, 1 vi, $86.00 Ask -
bioRxiv - Immunology 2019Quote: ... Specimens were finely minced between two scalpel blades and incubated for 15 minutes at 37°C with 600 U/mL collagenase IV (Worthington, Lakewood, NJ) and 20 ug/mL DNAse 1 (Roche ...
-
bioRxiv - Neuroscience 2019Quote: ... neural retinas were digested at 37°C for 9 minutes in papain solution and single cells were obtained following manufacturer instructions (Papain Dissociation System, Worthington Biochemical, USA). Living cells were counted using a hemocytometer after trypan blue staining and resuspended in PBS at a density of 1×105 cells/μl ...
-
bioRxiv - Neuroscience 2020Quote: Brain samples were minced with scissors and then incubated at 37 °C on a roller for 30 min in digest buffer containing 1.25 mg/mL Collagenase Type 4 (Worthington, Lakewood, NJ, USA) and 100 U/mL DNAseI (Sigma ...
-
bioRxiv - Genomics 2020Quote: ... Chromatin digestion was carried out at 30°C for 12 minutes with different concentrations of micrococcal nuclease (MNase, Worthington Biochemicals; cat# LS004798). The digestion was stopped by adding EDTA to final concentration of 10 mM ...
Product cited:
Chromatographically purified to be essentially homogeneous chromatographically and...Cat# LS004798, 45 ku, $211.00 Ask -
bioRxiv - Immunology 2021Quote: ... Cells were infected with lung homogenate supernatants at varying dilutions for 45 min at 37°C before the addition of Leibovitz L15 or MEM medium containing 0.9% agarose overlay containing Trypsin (Worthington Biochemical, NJ, USA), as previously described[54] ...
-
bioRxiv - Genomics 2022Quote: ... Nuclei were collected by centrifugation and DNase I-treated for 30 min at 37°C (Worthington, 30U per 5 x 106 cells). After DNase I digestion ...
-
bioRxiv - Molecular Biology 2022Quote: Five 10um cryostat slices of patient polyps were digested for 5 min at 37°C with a titration of MNase: 20 units/mL and 200 unit/mL of MNase (Worthington Biochemical Corp.) in MNase cleavage buffer (4 mM MgCl2 ...
-
bioRxiv - Neuroscience 2022Quote: ... which were immediately chopped and digested at 37°C with rotation in a solution containing 2mg/ml papain (Worthington Biochemical Corporation, LS003126) and 50 μg/ml DNase I in PBS ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003126, 100 mg, $88.00 Ask -
bioRxiv - Genomics 2023Quote: ... Tissue was coarsely chopped with a sterile scalpel and digested for 1hr at 34°C in a pH-equilibrated papain solution (Worthington, Lakewood, NJ) with constant CO2 flow over the enzyme solution ...
-
bioRxiv - Neuroscience 2023Quote: ... Retinas were detached from the retinal pigmented epithelium and incubated for 15 min at 37°C in a solution containing papain (10 U/ml, Worthington; Lakewood, NJ) and L-Cysteine hydrochloride (∼1 mg/mL ...
-
bioRxiv - Physiology 2024Quote: ... CD1 wild type) were harvested and enzymatically digested for 45 min at 37 °C with collagenase type II (520 U/ml, Worthington, NJ US) (20) ...
-
bioRxiv - Neuroscience 2023Quote: ... washed twice in HBSS and incubated during 30 min at 37°C in pre-warmed, oxygenated (95% O2, 5% CO2) Papain/DNase I (Worthington Biochemical Corporation) on a gyratory shaker (100 rpm) ...
-
bioRxiv - Neuroscience 2020Quote: ... After adding 0.9 mL pre-warmed HBSS containing 10 mg/mL Collagenase Type 2 (Worthington, UK) samples were incubated for 25 min at 37°C rotating every 5 min and then washed with 2 mL pre-warmed DMEM/F12 supplemented with 10% FBS ...
-
bioRxiv - Immunology 2020Quote: ... and incubated in 2 ml enzymatic solution containing 1.5 mg/ml collagenase type IV (Worthington, LS004188) and 0.5 mg/ml Dnase I (Roche ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
bioRxiv - Cell Biology 2021Quote: ... skin was minced and incubated in sterile 0.5% Collagenase Type 2 (Worthington Chemicals, Cat. No. LS004176) overnight at 37 °C in an atmosphere of 5% CO2 and 5% O2 ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Cancer Biology 2022Quote: ... The digestion was terminated with the addition of 2 ml Ovomucoid (0.7 mg/ml, Worthington, LS003086). Following digestion ...
Product cited:
Mucoprotein and antitryptic factor of egg white described by Lineweaver and Murray, J. Biol....Cat# LS003086, 2 gm, $280.00 Ask -
bioRxiv - Developmental Biology 2022Quote: ... HBSS was removed and 2 ml of freshly made 1 mg/ml collagenase (Worthington Biochemical, LS004194) in HBSS was added to each tube ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004194, 100 mg, $42.00 Ask -
bioRxiv - Genetics 2022Quote: ... blood was removed by perfusion and the heart was digested with collagenases 2 and 4 (Worthington) and Protease XIV (Sigma) ...
-
bioRxiv - Immunology 2021Quote: ... MLV-SARS-CoV-2 pseudovirus was activated with 10 µg/ml TPCK treated-Trypsin (Worthington Biochem) for 1 h at 37 °C ...
-
bioRxiv - Genetics 2022Quote: ... digested for 30 minutes with 245 units/ml type-2 collagenase (Worthington Biochemical Corp, Lakewood, USA) and 96 μg/ml soybean trypsin inhibitor (Sigma) ...
-
bioRxiv - Neuroscience 2022Quote: ... Single-cell suspensions were prepared by using Papain containing l-cysteine and EDTA (vial 2, Worthington), DNase I (vial 3 ...
-
bioRxiv - Immunology 2022Quote: ... The digestion was terminated with the addition of 2 ml Ovomucoid (0.7 mg/ml, Worthington, LS003086). Following digestion ...
Product cited:
Mucoprotein and antitryptic factor of egg white described by Lineweaver and Murray, J. Biol....Cat# LS003086, 2 gm, $280.00 Ask -
bioRxiv - Immunology 2022Quote: Lung tissue was harvested and digested with type 2 collagenase (1 mg/ml, Worthington; cat. LS004177) for 45 min at 37°C to acquire cell suspensions ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004177, 5 gm, $900.00 Ask -
bioRxiv - Microbiology 2023Quote: ... and 1 μg/ml L-1-tosylamido-2-phenylethyl chloromethyl ketone (TPCK)-treated trypsin (Worthington Biochemicals). Plaque forming units (PFU ...
-
bioRxiv - Biochemistry 2023Quote: ... trypsin (L-1-tosylamido-2-phenylethyl chloromethyl ketone treated) was obtained from Worthington (New Jersey, USA); DPP-IV was obtained from AT Gen (Korea) ...
-
bioRxiv - Cell Biology 2022Quote: ... Tissue was then incubated with F12K medium containing 2 mg/ml collagenase type IV (Worthington LS004188) at 37°C with pipetting every 5-10 min until most of the glandular cells were released and appeared in solution as clusters ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... the samples were washed with 1 ml inhibitor solution with 2% trypsin inhibitor (Worthington Biochemical, LS00308) and resuspended in 1 ml of the same solution for trituration ...
-
bioRxiv - Cell Biology 2023Quote: ... and transferred into a digestion buffer consisting of 2 mg/mL collagenase II (LS004176, Worthington Biochemical) and 0.3 mM calcium chloride in CIB ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Cell Biology 2021Quote: ... A single cell suspension of vascular stromal cells was isolated from minced iBAT (using scissors) digested for 90 min with collagenase IV (3 mg mL-1, Worthington, Lakewood NJ, USA) at 37 °C under gentle agitation16 ...
-
bioRxiv - Microbiology 2019Quote: ... followed by a 10× dilution using 100 mM Tris-HCl (pH = 8.0) and tryptic digestion under 37°C for 18 hours using 1 µg of trypsin per well (Worthington Biochemical Corp., Lakewood, NJ). Digested peptides were desalted using a panel of lab-made 96-channel filter tips generated by inserting 96 20 µl filter tips into a 96-well cover mat and stacking each filter tip with 5 mg of 10-μm C18 column beads ...
-
bioRxiv - Immunology 2021Quote: ... Collected lobes were incubated for one hour at 37°C in 10 mL of cRPMI with 1500 units of 260 units/mg type 1 collagenase (Worthington Biochemical, Cat. #LS004194) per lobe and pressed through a 35 µm filter ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004194, 100 mg, $42.00 Ask -
bioRxiv - Cell Biology 2022Quote: Cells were isolated from semitendinosus muscles of 10 Belgian Blue cattle (Supplementary Table 1) by collagenase digestion (CLSAFA, Worthington; 1 h; 37 °C), filtration with 100 μm cell strainers ...
-
bioRxiv - Microbiology 2021Quote: ... followed by 10X dilution into 50 mM ABC (pH 8.0) and tryptic digestion at 37°C for 20 hr with shaking using 1 μg of trypsin (Worthington Biochemical Corp., Lakewood, NJ) per 50 μg protein ...
-
bioRxiv - Cancer Biology 2022Quote: ... Human brain tissue was manually minced using razor blades and enzymatically dissociated at 37°C for 30 minutes with 1 mg mL-1 collagenase-4 (#LS004188, Worthington Biochemical Corporation, USA) and 250 U mL-1 DNAse1 (#10104159001 ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
Machine Learning Ensemble Directed Engineering of Genetically Encoded Fluorescent Calcium IndicatorsbioRxiv - Bioengineering 2023Quote: ... Tissue was minced until 1mm pieces remained, then lysed in equilibrated (37°C, 5% CO2) enzyme (20 U/mL Papain (Worthington Biochemical Corp; LK003176) in 5mL of EBSS (Sigma ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003176, 1 vi, $29.00 Ask -
bioRxiv - Cancer Biology 2023Quote: ... Brain tissues were manually minced using razor blades and enzymatically dissociated at 37°C for 30 min with 1 mg/mL collagenase type IV (#LS004188, Worthington Biochemical Corporation, USA) and 250 U/mL DNase 1 (#10104159001 ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
bioRxiv - Cancer Biology 2023Quote: ... Human brain tissue was manually minced using razor blades and enzymatically dissociated at 37°C for 30 minutes with 1 mg/ml collagenase-4 (#LS004188, Worthington Biochemical Corporation, USA) and 250 U/ml DNAse1 (#10104159001 ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
bioRxiv - Bioengineering 2024Quote: ... Tissue was minced until 1mm pieces remained, then lysed in equilibrated (37°C, 5% CO2) enzyme (20 U/mL Papain (Worthington Biochemical Corp; LK003176) in 5mL of EBSS (Sigma ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003176, 1 vi, $29.00 Ask -
bioRxiv - Bioengineering 2024Quote: ... Tissue was minced until 1mm pieces remained, then lysed in equilibrated (37°C, 5% CO2) enzyme (20 U/mL Papain (Worthington Biochemical Corp; LK003176) in 5mL of EBSS (Sigma ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003176, 1 vi, $29.00 Ask -
bioRxiv - Physiology 2020Quote: ... oocytes contained in 1-3 mL of ovary lobes extracted via survival surgery were isolated by enzymatic digestion with collagenase type II (Worthington Biochemical Corp., NJ, USA) under mechanical agitation ...
-
bioRxiv - Molecular Biology 2022Quote: Calvarial osteoblasts were obtained from 3 to 5-day-old C57BL/6 mice by sequential enzyme digestion [1 mg/ml collagenase type II (Worthington Biochemical, Lakewood, NJ, USA) in Hanks’ balanced salt solution (Life Technologies ...
-
bioRxiv - Cell Biology 2019Quote: ... Tibiae and femora from each mouse were combined and incubated with 4 ml of collagenase solution (3 mg/ml Collagenase Type IV in PBS, Worthington Biochemical Corp., Lakewood, NJ) inside a 5% CO2 chamber at 37°C for 15 min under continuous agitation ...
-
bioRxiv - Cell Biology 2019Quote: ... each consisting of calvaria from 6 pups were digested in 10 ml collagenase solution (1 mg/ml Collagenase type I and type II (1:3 ratio, Worthington Biochemical Corp., Lakewood, NJ), 1 mM CaCl2 ...
-
bioRxiv - Cancer Biology 2024Quote: The ovarian cancer tissues were incubated in DMEM high glucose culture medium supplemented with 3 mg/ml collagenase D (Worthington-biochem, Lakewood, NJ, USA) at 37°C for 45 min ...