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Citations for Worthington Biochemical :
351 - 400 of 514 citations for 7 Hydroxy 2 3 dihydro 1H cyclopenta c chromen 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: Nuclei from 5×105 cells were aliquoted into microcentrifuge tubes and incubated at 37°C for 5 min with varying amounts of DNase (0 to 75 U; Worthington, LS006344). Digestion was stopped by addition of an equal volume of termination buffer [10 mM Tris (Sigma ...
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bioRxiv - Neuroscience 2019Quote: ... following genotyping or embryonic day 18 embryos (rat) and dissociated enzymatically for 20 min at 37 °C in HBSS supplemented with 0.25% (w/v) trypsin (Worthington Cat# LS003707), followed by mechanical dissociation via trituration with fire-polished glass Pasteur pipettes ...
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bioRxiv - Neuroscience 2020Quote: ... the cells were washed with PBS 48 hours after viral transduction and digested for 30 minutes at 37°C with a papain (Worthington, LS003119) solution (2mg/ml KnockOutTM DMEM without calcium ...
Product cited:
Supplied as a lyophilized powder prepared from a 2X crystalline suspension, Code: PAP. To...Cat# LS003119, 100 mg, $100.00 Ask -
bioRxiv - Immunology 2019Quote: Tumour bearing mice were sacrificed and tumours were excised and digested at 37°C for 30 min using a cocktail of 1 mg/ml collagenase type IV (Worthington Biochemicals) and 0.02 mg/ml DNase (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2019Quote: ... incubated in 37°C Trypsin-DNase (1g/L DNase-Worthington Biochemical-, 10g/L Trypsin - Worthington Biochemical-, 6mM NaOH in CMF-PBS) for 5 minutes and triturated 10x in 1ml RT DNase buffer (0.5g/L DNase ...
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bioRxiv - Physiology 2022Quote: ... The hearts were removed and perfused at 37 °C in Langendorff method with nominally Ca2+-free Tyrode’s solution containing 0.5 mg/ml collagenase (Type II; Worthington, Lakewood, NJ, USA) and 0.1 mg/ml protease (type XIV ...
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bioRxiv - Cancer Biology 2022Quote: ... tissue samples were cut into small pieces and enzymatically digested for 30 min at 37 °C on a shaker with 5000U collagenase IV (Worthington, LS004188), 15 KU DNAse I (Sigma ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
bioRxiv - Cancer Biology 2023Quote: Tumor-bearing mice were sacrificed and tumors excised and digested at 37°C for 30 min using a cocktail of 1 mg/ml collagenase type IV (Worthington Biochemicals) and 0.02 mg/ml DNase (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2023Quote: ... urogenital ridges and adrenal glands were enzymatically dissociated at 37°C between 30 and 40 minutes using the Papain dissociation system (Worthington LK003150). Cells were resuspended in DMEM (Gibco 11885084 ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Cancer Biology 2023Quote: The largest tumors from female C3-TAg and PyMT mice were minced and dissociated for up to 120 min at 37°C in a mixture of 1 mg/ml Collagenase (Worthington, LS004188), 20 U/ml DNase (Invitrogen ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... was dissected on ice and minced using two razor blades in a few drops of pre-warmed (37°C) Hibernate A medium containing 20 units/mL papain (Worthington LK003178). Minced tissue was incubated in 3.125 mL pre- warmed papain solution for 10 minutes at 37°C ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003178, 5 vi, $98.00 Ask -
bioRxiv - Bioengineering 2024Quote: ... hippocampi were dissected from brains of newborn pups (P0) and digested for 20 min at 37 °C with 10 units of papain (Worthington, USA), followed by gentle mechanical trituration ...
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bioRxiv - Immunology 2021Quote: ... mechanically cut into small pieces and digested with 0,1 % Collagenase type 2 (265U/mL; Worthington) and DNase I (250U/mL ...
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bioRxiv - Cell Biology 2020Quote: ... minced lung tissue was transferred to 25 mL pre-warmed collagenase (2 mg/mL, Worthington Biochemical Corporation ...
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bioRxiv - Bioengineering 2021Quote: ... followed by 2 h digestion with 300 U ml-1 type I collagenase (Worthington Biochemical). Dissociated cells were seeded in culture plates ...
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bioRxiv - Developmental Biology 2022Quote: ... were incubated in 2 mg/ml collagenase type II (260 U/mg, Worthington, Lakewood, NJ) in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The perfusate was then switched to Tyrode’s solution containing collagen type 2 (Worthington, Columbus, OH) and protease type 14 (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... 200 μL of 20 mg/mL of type 2 collagenase in L15 (Cat.No. NC9870009; Worthington) were added and incubated on an orbital rotator at 28°C for 35 min ...
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bioRxiv - Immunology 2020Quote: To prepare single-cell suspensions lungs were finely chopped with scissors in a 48 well plate and treated with 3 mg ml-1 collagenase III (Worthington, Lakewood, NJ), 5 μg ml-1 DNAse ...
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bioRxiv - Neuroscience 2023Quote: ... Cell pellet was collected by centrifuged at 300 g for 5 min and then resuspended in 3 mL Ovomucoid protease inhibitor with FBS (Inhibitor-BSA Vial [LK003182], Worthington, Lakewood, NJ). To remove the debris ...
Product cited:
Ovomucoid protease inhibitor and bovine serum albumin which is 0.22 micron filtered and...Cat# LK003182, 1 vi, $86.00 Ask -
bioRxiv - Immunology 2019Quote: ... Specimens were finely minced between two scalpel blades and incubated for 15 minutes at 37°C with 600 U/mL collagenase IV (Worthington, Lakewood, NJ) and 20 ug/mL DNAse 1 (Roche ...
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bioRxiv - Neuroscience 2019Quote: ... neural retinas were digested at 37°C for 9 minutes in papain solution and single cells were obtained following manufacturer instructions (Papain Dissociation System, Worthington Biochemical, USA). Living cells were counted using a hemocytometer after trypan blue staining and resuspended in PBS at a density of 1×105 cells/μl ...
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bioRxiv - Genomics 2020Quote: ... Chromatin digestion was carried out at 30°C for 12 minutes with different concentrations of micrococcal nuclease (MNase, Worthington Biochemicals; cat# LS004798). The digestion was stopped by adding EDTA to final concentration of 10 mM ...
Product cited:
Chromatographically purified to be essentially homogeneous chromatographically and...Cat# LS004798, 45 ku, $211.00 Ask -
bioRxiv - Immunology 2021Quote: ... Cells were infected with lung homogenate supernatants at varying dilutions for 45 min at 37°C before the addition of Leibovitz L15 or MEM medium containing 0.9% agarose overlay containing Trypsin (Worthington Biochemical, NJ, USA), as previously described[54] ...
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bioRxiv - Genomics 2022Quote: ... Nuclei were collected by centrifugation and DNase I-treated for 30 min at 37°C (Worthington, 30U per 5 x 106 cells). After DNase I digestion ...
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bioRxiv - Molecular Biology 2022Quote: Five 10um cryostat slices of patient polyps were digested for 5 min at 37°C with a titration of MNase: 20 units/mL and 200 unit/mL of MNase (Worthington Biochemical Corp.) in MNase cleavage buffer (4 mM MgCl2 ...
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bioRxiv - Neuroscience 2022Quote: ... which were immediately chopped and digested at 37°C with rotation in a solution containing 2mg/ml papain (Worthington Biochemical Corporation, LS003126) and 50 μg/ml DNase I in PBS ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003126, 100 mg, $88.00 Ask -
bioRxiv - Genomics 2023Quote: ... Tissue was coarsely chopped with a sterile scalpel and digested for 1hr at 34°C in a pH-equilibrated papain solution (Worthington, Lakewood, NJ) with constant CO2 flow over the enzyme solution ...
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bioRxiv - Neuroscience 2023Quote: ... washed twice in HBSS and incubated during 30 min at 37°C in pre-warmed, oxygenated (95% O2, 5% CO2) Papain/DNase I (Worthington Biochemical Corporation) on a gyratory shaker (100 rpm) ...
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bioRxiv - Neuroscience 2023Quote: ... Retinas were detached from the retinal pigmented epithelium and incubated for 15 min at 37°C in a solution containing papain (10 U/ml, Worthington; Lakewood, NJ) and L-Cysteine hydrochloride (∼1 mg/mL ...
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bioRxiv - Physiology 2024Quote: ... CD1 wild type) were harvested and enzymatically digested for 45 min at 37 °C with collagenase type II (520 U/ml, Worthington, NJ US) (20) ...
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bioRxiv - Immunology 2019Quote: ... DRGs were further digested with 1 mL of collagenase type II/dispase solution (HBSS/10 mM HEPES/4 mg/mL collagenase type II (Worthington)/5 mg/mL dispase (Thermo Fisher) ...
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bioRxiv - Bioengineering 2020Quote: ... Cell suspensions were harvested by mechanical dissection and Collagenase IV Cocktail digestion (formulated as 7.4 kU Collagenase Type 4, 53 kU Deoxyribonuclease I, 20 mg Soybean Trypsin Inhibitor in 10 mL cocktail; Worthington Biochemical). The suspensions were passed through 40 μm cell strainers to ensure singularity ...
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bioRxiv - Cell Biology 2019Quote: ... The muscles were digested in 4 ml:1 g of tissue wet weight in a solution of 0.5% Collagenase II (Worthington Biochemicals), 1.2 U/ml Dispase (Worthington Biochemicals) ...
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bioRxiv - Developmental Biology 2023Quote: ... washed with PBS and dissociated into single cells using collagenase type 4 enzyme at 200Units/ml (Worthington, Lakewood, NJ, US) for 10min at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... The muscles were digested in 4 ml:1 g of tissue wet weight in a solution of 0.5% Collagenase II (Worthington Biochemicals), 1.2 U/ml Dispase (Worthington Biochemicals) ...
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bioRxiv - Neuroscience 2020Quote: ... After adding 0.9 mL pre-warmed HBSS containing 10 mg/mL Collagenase Type 2 (Worthington, UK) samples were incubated for 25 min at 37°C rotating every 5 min and then washed with 2 mL pre-warmed DMEM/F12 supplemented with 10% FBS ...
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bioRxiv - Immunology 2020Quote: ... and incubated in 2 ml enzymatic solution containing 1.5 mg/ml collagenase type IV (Worthington, LS004188) and 0.5 mg/ml Dnase I (Roche ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
bioRxiv - Cell Biology 2021Quote: ... skin was minced and incubated in sterile 0.5% Collagenase Type 2 (Worthington Chemicals, Cat. No. LS004176) overnight at 37 °C in an atmosphere of 5% CO2 and 5% O2 ...
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bioRxiv - Cancer Biology 2022Quote: ... The digestion was terminated with the addition of 2 ml Ovomucoid (0.7 mg/ml, Worthington, LS003086). Following digestion ...
Product cited:
Mucoprotein and antitryptic factor of egg white described by Lineweaver and Murray, J. Biol....Cat# LS003086, 2 gm, $280.00 Ask -
bioRxiv - Developmental Biology 2022Quote: ... HBSS was removed and 2 ml of freshly made 1 mg/ml collagenase (Worthington Biochemical, LS004194) in HBSS was added to each tube ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004194, 100 mg, $42.00 Ask -
bioRxiv - Immunology 2021Quote: ... MLV-SARS-CoV-2 pseudovirus was activated with 10 µg/ml TPCK treated-Trypsin (Worthington Biochem) for 1 h at 37 °C ...
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bioRxiv - Genetics 2022Quote: ... digested for 30 minutes with 245 units/ml type-2 collagenase (Worthington Biochemical Corp, Lakewood, USA) and 96 μg/ml soybean trypsin inhibitor (Sigma) ...
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bioRxiv - Neuroscience 2022Quote: ... Single-cell suspensions were prepared by using Papain containing l-cysteine and EDTA (vial 2, Worthington), DNase I (vial 3 ...
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bioRxiv - Immunology 2022Quote: ... The digestion was terminated with the addition of 2 ml Ovomucoid (0.7 mg/ml, Worthington, LS003086). Following digestion ...
Product cited:
Mucoprotein and antitryptic factor of egg white described by Lineweaver and Murray, J. Biol....Cat# LS003086, 2 gm, $280.00 Ask -
bioRxiv - Immunology 2022Quote: Lung tissue was harvested and digested with type 2 collagenase (1 mg/ml, Worthington; cat. LS004177) for 45 min at 37°C to acquire cell suspensions ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004177, 5 gm, $900.00 Ask -
bioRxiv - Microbiology 2023Quote: ... and 1 μg/ml L-1-tosylamido-2-phenylethyl chloromethyl ketone (TPCK)-treated trypsin (Worthington Biochemicals). Plaque forming units (PFU ...
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bioRxiv - Neuroscience 2023Quote: ... the samples were washed with 1 ml inhibitor solution with 2% trypsin inhibitor (Worthington Biochemical, LS00308) and resuspended in 1 ml of the same solution for trituration ...
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bioRxiv - Biochemistry 2023Quote: ... trypsin (L-1-tosylamido-2-phenylethyl chloromethyl ketone treated) was obtained from Worthington (New Jersey, USA); DPP-IV was obtained from AT Gen (Korea) ...
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bioRxiv - Cell Biology 2022Quote: ... Tissue was then incubated with F12K medium containing 2 mg/ml collagenase type IV (Worthington LS004188) at 37°C with pipetting every 5-10 min until most of the glandular cells were released and appeared in solution as clusters ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask