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Citations for Worthington Biochemical :
251 - 300 of 407 citations for Alpha Cyclodextrin Solution 5% w v since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... Approximately 40-60 wing discs were dissociated in 250 μL of Papain (20 U/mL) and DNase I (200 U/mL) in Earle’s Buffered Salt Solution (all from Worthington Biochemical), at 25°C for 40 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... and dissected kidneys were minced and incubated in digestion solution for 15 min at 37°C (collagenase II 300 U/ml (Worthington), pronase E 1 mg/ml (Sigma) ...
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bioRxiv - Developmental Biology 2020Quote: ... followed by quenching of the enzyme using 1 mg/ml ovomucoid/bovine serum albumin solution with 0.1 mg/ml DNase I (Worthington LK003150). Cells were passed through a 70 μm strainer (PluriSelect 43-10070-70 ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Immunology 2021Quote: ... tissue disaggregation was performed by finely chopping liver samples (~1-2 g) in 10 ml digestion solution containing 1 mg/ml Collagenase IV (Worthington) and 20 μg/ml DNAse1 (Roche ...
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bioRxiv - Bioengineering 2020Quote: ... acellular PCL and GA or GP crosslinked dMEP scaffolds (n = 4/group) were digested in 2 mg/mL collagenase type 2 solution (Worthington) for 24 h at 37°C ...
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bioRxiv - Immunology 2022Quote: Lung tissue was minced and digested in a RPMI 1640 solution with Type 2 collagenase (0.5 mg/mL) (Worthington Biochemical) and 5 units/mL DNAse (Sigma ...
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bioRxiv - Developmental Biology 2022Quote: ... tissue was washed twice with 0.3ml ice- cold CMF PBS and incubated in 0.3ml papain solution (20U/ml, 1mM L-Cysteine and 0.5mM EDTA; Worthington Biochemical Corporation) at 37°C for 40 minutes ...
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bioRxiv - Developmental Biology 2021Quote: ... anterior tips of ovaries we transferred to a 1.5 ml tube and were incubated in dissociation solution (0.25 mg/ml Type I Collagenase, 0.4 mg/ml elastase (Worthington Biochemical #LS002290) in Cell Dissociation Buffer (Thermo Fisher Scientific #13151014) ...
Product cited:
Two times crystallized, (Code: ESL), supplied as a dialyzed, lyophilized powder. The enzyme...Cat# LS002290, 25 mg, $55.00 Ask -
bioRxiv - Immunology 2022Quote: ... Aortae were dissected and digested in a solution containing of 1200 U/ml collagenase II (Worthington Biochemicals, Lakewood, NJ, USA), 60 U/ml DNase (Sigma Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... and Plp1-TAT-DTR mice (for P42_new IHCs) were dissected out and first digested in a choline chloride solution containing 20 U/mL papain (Cat#: LK003178, Worthington) and 100 U/mL DNase I (Cat# ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003178, 5 vi, $98.00 Ask -
bioRxiv - Bioengineering 2023Quote: ... The 3D matrices were then digested using a solution of type IV collagenase (6 mg/mL; Worthington Biochemical Corporation, USA). Subsequently ...
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bioRxiv - Bioengineering 2023Quote: ... the 3D collagen matrices were digested using a 6 mg/ml solution of type IV collagenase (Worthington Biochemical Corporation, USA). The digested collagen matrices were then incubated with Alcian blue 8GX (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... Tissue was then washed in Hanks Balanced Salt Solution with Ca2+ or Mg2+ briefly and then digested in 1mg/ml collagenase (Worthington) for 30 minutes at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... Tissues were then incubated at 37°C for 3 minutes in a solution of HBSS/0.2% glucose with 10 mg/ml trypsin (Worthington Biochemical) and 1 mg/ml Deoxyribonuclease I (Worthington Biochemical) ...
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bioRxiv - Immunology 2024Quote: ... Enzymatic digestion was stopped with 1 mL of 10X hi ovomucoid inhibitor solution and 20 µL 0.4% DNase (Worthington, #LS002007) diluted in 10 mL inhibitor stock solution (ISS) ...
Product cited:
Chromatographically purified. A lyophilized powder with glycine as a stabilizer.Cat# LS002007, 100 mg, $350.00 Ask -
bioRxiv - Neuroscience 2023Quote: Longitudinal muscle myenteric plexus layers were collected and placed in a digestive KREBS solution buffer with 1.3 mg/mL collagenase type II (Worthington, LS004176) and 0.3 mg/mL BSA (Sigma ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Cell Biology 2023Quote: ... the hippocampi were washed twice with cold HBSS and individually incubated at 37°C for 20 min in Papain dissociation solution (45 U of papain (Worthington), 0.01% deoxyribonuclease (DNase) ...
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bioRxiv - Genomics 2023Quote: ... Cells were then filtered through a 70µm cell strainer into an inactivation solution containing albumin-ovomucoid inhibitor (Worthington, Cat#LK003182), DNAseI (1mg/mL ...
Product cited:
Ovomucoid protease inhibitor and bovine serum albumin which is 0.22 micron filtered and...Cat# LK003182, 1 vi, $86.00 Ask -
bioRxiv - Cell Biology 2023Quote: ... and individually incubated at 37°C for 15 to 20 min in a sterile solution of 45 U papain (Worthington), 0.01% DNase (Sigma) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Ganglia were cut into small pieces and cells were dissociated by incubating ganglia pieces in enzyme solution (10 mg/mL collagenase IV (Worthington), in 1XHBS (HEPES buffered saline ...
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bioRxiv - Cell Biology 2023Quote: ... and individually incubated at 37 °C for 15 to 20 min in a sterile solution of 45 U papain (Worthington), 0.01% DNase (Sigma) ...
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bioRxiv - Developmental Biology 2022Quote: Hearts from E14.5 embryos were isolated and incubated 30 minutes at 37°C on a rotating shaker with 200 µl of digestion solution per embryo consisting out of 0.05 mg/ml DNase I (Worthington, 2139) and 1 mg/ml Collagenase I (Worthington ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... using a scalpel and surgical scissors and directly placed into 2mL of enzymatic solution: Collagenase II – 500U/mL (WOLS04177, Worthington), DNase I – 1,6U/mL (11284932001 ...
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bioRxiv - Bioengineering 2022Quote: ... In vitro degradation of lignin composites was determined by submerging lignin composites in the solution of collagenase type II (0.5 U/mL, cat# CLS-2, Worthington Biochemical) with 1 mM CaCl2 in serum-free culture medium ...
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bioRxiv - Neuroscience 2024Quote: ... the retinas were incubated for 7 min at 37°C in a solution of papain and deoxyribonuclease (DNase) (Worthington, #LK003172). Incubation was followed by trituration of the mixture to dissociate the cells ...
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bioRxiv - Neuroscience 2024Quote: ... The neocortices were isolated from pups on day 0-1 in cold Earle’s Balanced Salt Solution (EBSS) and incubated for 15 min at 37°C in EBSS containing 10 U/ml papain (Worthington), followed by gentle dissociation in PBS containing 1.5 mg/ml BSA ...
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bioRxiv - Immunology 2024Quote: Lung tissue was minced and digested in an RPMI 1640 solution with type 2 collagenase (0.5 mg/mL) (Worthington Biochemical) and 5 units/mL DNAse (MilliporeSigma ...
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bioRxiv - Neuroscience 2024Quote: ... samples were pre-cut with small scissors and diluted and digested in 2:3 stock solution of CollagenaseI (10 U/ml, Worthington), Collagenase IV (400 U/ml ...
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bioRxiv - Systems Biology 2024Quote: ... WT and Elavl2 knockout EBs were collected into Eppendorf tubes where the medium was replaced with papain solution containing 20 U/mL papain and 67 U/mL DNase (Worthington). Tubes were incubated for 45-60 minutes at 37° C under constant agitation ...
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Sex-specific perturbations of neuronal development caused by mutations in the autism risk gene DDX3XbioRxiv - Neuroscience 2024Quote: ... Cortices from single embryos were isolated in ice-cold Hank’s balanced salt solution (HBSS) and then digested with pre-warmed papain containing DNase (Worthington, #LK003150) at 37°C and 5% CO2 for 30 min ...
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bioRxiv - Neuroscience 2024Quote: ... minced pieces (1-2 mm3) of isolated cerebral cortices were enzymatically digested in Hank’s buffered saline solution containing papain (20 U/ml, Worthington Biochemical), L-cysteine (1 mM ...
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DDK regulates replication initiation by controlling the multiplicity of Cdc45-GINS binding to Mcm2-7bioRxiv - Cell Biology 2020Quote: ... Proteins were released from the DNA by incubation with 5 U of DNA I (Worthington) in 10 mL of 25 mM HEPES-KOH (pH 7.6) ...
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bioRxiv - Microbiology 2022Quote: Lungs were collected and digested at 37 with 5 mg/mL of collagenase I (Worthington) and 1 mg/mL of DNase I (Roche ...
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bioRxiv - Genetics 2023Quote: ... 5 µg/mL of Oxyrase (Oxyrase Inc.) and 150 U/mL Catalase (Worthington Bio Crop), or on 5% Sheep’s blood agar plates at 370C in a 5% CO2 atmosphere ...
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bioRxiv - Neuroscience 2023Quote: ... and the pellet was resuspended in 5 mL dissociation media containing 165U of Papain (Worthington) and DNAse type I (40 µg/mL ...
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bioRxiv - Immunology 2024Quote: ... before enzymatic digestion in 0.5mL DMEM containing 5 mg/mL type II collagenase (Worthington, # LS004202) and 0.2 mg/mL DNase I (Roche ...
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bioRxiv - Neuroscience 2021Quote: ... Hippocampi were dissected in ice-cold Hank’s balanced salts solution (HBSS) and kept on ice until digestion with 0.2 μm-filtered 10 U/mL papain (Worthington Biochemical Corporation) in HBSS in a 37°C water bath for 20 min ...
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bioRxiv - Plant Biology 2020Quote: ... A 5 to 7-day-old protonemal tissue culture was scraped from solid plates and added to a 466 mM mannitol solution with 2% Driselase and Cellulase (21 unit/mL, Worthington CEL). The mixture was gently agitated for 1 h at room temperature (RT) ...
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bioRxiv - Microbiology 2022Quote: ... cut into small pieces and then digested for 30 minutes under gentle agitation at 37 °C with 7 mL of the following solution: type III collagenase (Worthington Biochemical), 4 mg/mL ...
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bioRxiv - Physiology 2022Quote: ... after intracardiac perfusion of Phosphate Buffer Saline followed by a solution of HBSS containing type II collagenase at 2 mg/ml (Worthington, USA). Specific diets described below were introduced the day of the first dimerizer injection and maintained until sacrifice.
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bioRxiv - Neuroscience 2021Quote: ... 2–3 randomly selected hSS per hiPSC line were pooled in a 1.5-ml Eppendorf tube in the dissociation solution (10U/mL papain (Worthington Biochemical, LS003119), 1 mM EDTA ...
Product cited:
Supplied as a lyophilized powder prepared from a 2X crystalline suspension, Code: PAP. To...Cat# LS003119, 100 mg, $100.00 Ask -
bioRxiv - Immunology 2021Quote: Single cell suspensions were generated from Achilles tendons of MaFIA mice by enzymatic digestion with a solution containing 1 mg/mL collagenase I (Cat. # LS004196, Worthington Biochemical) and 5 mg/mL collagenase IV (Cat ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004196, 1 gm, $215.00 Ask -
bioRxiv - Cell Biology 2021Quote: ... Cortices were digested at room temperature for 1 hour using papain solution composed of 20 units/ml papain (Worthington Biochem, LS003126), 1.5 mM CaCl2 (Sigma Aldrich ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003126, 100 mg, $88.00 Ask -
bioRxiv - Neuroscience 2020Quote: ... and the supernatant aspirated before the tissue was incubated in a collagenase solution containing 2 mg/mL collagenase type II (CLS2; Worthington Biochemical) in 5 mL 1X HBSS in a 37°C rocking water bath for 10 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... organoids (single organoids cultured in a 96-well plate or up to 8 organoids cultured in a 6-well plate) were incubated with an enzyme solution containing 10U/ml papain (Worthington, LS003127), 1x EBSS (Sigma-Aldrich ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003127, 1 gm, $595.00 Ask -
bioRxiv - Bioengineering 2023Quote: ... constructs were extracted from devices and incubated on an orbital shaker for 1 hour in a solution of 0.15% type II collagenase (Worthington Biochemical Corporation) in DMEM ...
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bioRxiv - Neuroscience 2024Quote: ... with 2 mL pre-warmed digestion enzyme in ACSF solution (Papain, 25 unit/mL and DNase I, 55 unit/mL, Worthington Biochemical). For spinal dorsal horn neuronal atlas construction ...
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bioRxiv - Immunology 2024Quote: ... mice were perfused with 1X PBS and the CNS was isolated into 10 mL of enzyme digestion solution consisting of 75 µL Papain suspension (Worthington, #LS003126) diluted in enzyme stock solution (ESS ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003126, 100 mg, $88.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... Homogenate was digested for 15 minutes in enzymatic solution (0.2 µm filter sterilised 4.5 U/mL papain [Worthington Biochemicals], 1× DNAse [Worthington Biochemicals], in PBS) at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... PBS was supplemented with DNase solution (containing a final concentration of 200U/ml DNase [Worthington], 2.5 mM MgCl2, 500 mM CaCl2). After the third wash ...