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Citations for Worthington Biochemical :
251 - 300 of 322 citations for 3 hydroxy 20 oxopregn 5 en 21 yl acetate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated for 5 minutes at RT in fetal calf serum complemented with DNase I (Worthington), followed by mechanical dissociation ...
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bioRxiv - Immunology 2021Quote: Perfused lungs were finely minced and digested in 5 mL of a 1.78mg/mL Collagenase IV (Worthington) solution in PBS (with Ca2+ ...
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bioRxiv - Cancer Biology 2020Quote: ... tissue was enzymatically digested with 5 mL of papain digestion solution (0.94 mg/mL papain (Worthington #LS003119), 0.48 mM EDTA ...
Product cited:
Supplied as a lyophilized powder prepared from a 2X crystalline suspension, Code: PAP. To...Cat# LS003119, 100 mg, $100.00 Ask -
Interactions with stromal cells promote a more oxidized cancer cell redox state in pancreatic tumorsbioRxiv - Cancer Biology 2020Quote: ... and digested in 5 mL of PBS containing cold 1 mg/mL collagenase I (Worthington Biochemical LS004194), 3mg/mL dispase II (Roche 04942078001 ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004194, 100 mg, $42.00 Ask -
bioRxiv - Immunology 2020Quote: ... Mice were immunized as indicated in each Figure with 5 μg low-endotoxin OVA (Worthington Biochemical Corporation) together with 50 μg papain (P4762 ...
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bioRxiv - Physiology 2024Quote: ... bubbled with 95% O2/5% CO2) containing collagenase type I (1.5 mg/ml, Worthington, Lakewood, NJ, USA) and 1% bovine serum albumin ...
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bioRxiv - Neuroscience 2024Quote: ... The DRG pieces were incubated in 5 ml HBSS containing 2 mg/mL Stemzyme (LS004106, Worthington Biochemical) and DNAse I (LS001239 ...
Product cited:
A specialized combination of Animal Free Clostridium histolyticum collagenase (Code: CLSAFB) and...Cat# LS004106, 50 mg, $110.00 Ask -
bioRxiv - Immunology 2023Quote: ... Mice were immunized as indicated in each Figure with 5 µg low-endotoxin OVA (Worthington Biochemical Corporation) mixed with either 50 µg papain (P4762 ...
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bioRxiv - Neuroscience 2020Quote: ... Acute bilateral fastigial nuclei were quickly excised with a sharp knife from 2 or 3 cerebellar slices and were then enzymatically digested with cysteine (2 mM)-supplemented papain (40 U/ml, Worthington, Lakewood, NJ) and chondroitinase-ABC (1 U/ml ...
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bioRxiv - Immunology 2020Quote: To prepare single-cell suspensions lungs were finely chopped with scissors in a 48 well plate and treated with 3 mg ml-1 collagenase III (Worthington, Lakewood, NJ), 5 μg ml-1 DNAse ...
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bioRxiv - Cell Biology 2024Quote: ... the RPE cells were washed at least 3 times with PBS to remove the dead cells and dissociated using Papain (Worthington Biochemical Corporation) for 30mins-1h at 37 °C following the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2021Quote: ... followed by 10X dilution into 50 mM ABC (pH 8.0) and tryptic digestion at 37°C for 20 hr with shaking using 1 μg of trypsin (Worthington Biochemical Corp., Lakewood, NJ) per 50 μg protein ...
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bioRxiv - Neuroscience 2023Quote: ... the cortical tissue was incubated with constant shaking at 37°C for 40 minutes in 1 ml of papain (20 U/ml) dissolved in the isolating solution supplemented with 60 µl of DNase (both from Worthington, Lakewood, NJ, USA). During the enzymatic dissociation ...
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bioRxiv - Developmental Biology 2023Quote: ... Tissue fragments were incubated in 1.35 mL PBS plus 150 uL 20 mg/mL Collagenase Type I (Worthington CLS-1 dissolved in PBS) and 15 uL 20 mg/ml DNase I (Worthington D dissolved in PBS ...
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bioRxiv - Cell Biology 2024Quote: ... Each leaflet was incubated for 20 min at 37 °C in 2 mg/mL type II collagenase (Worthington Biochemical Corp., Lakewood, NJ, USA) in cell culture medium (advanced Dulbecco’s modified Eagle’s medium (Ad DMEM ...
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bioRxiv - Cancer Biology 2021Quote: ... Right lung lobes were minced and digested with 5 mL digestion buffer: Collagenase 4 (200U/mL, Worthington Biochemicals), DNase I (100 ug/mL ...
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bioRxiv - Immunology 2024Quote: ... Remaining intestinal pieces were then incubated in HBSS containing 5% FBS and 150U/ml collagenase type 2 (Worthington) at 37°C with stirring for 45min to isolate LP cells ...
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bioRxiv - Cell Biology 2023Quote: ... Half of the samples were treated with a final concentration of 5 μg/mL bacterial collagenase (Worthington Biochemical) for 3 d at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: ... A single cell suspension of vascular stromal cells was isolated from minced iBAT (using scissors) digested for 90 min with collagenase IV (3 mg mL-1, Worthington, Lakewood NJ, USA) at 37 °C under gentle agitation16 ...
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bioRxiv - Immunology 2021Quote: ... Lungs were excised and processed by mincing with scissors followed by enzymatic digestion using 180 units/ml collagenase IV and 20 µg/ml DNase I (Worthington biochemical LS004188 and LS002139) in 5 ml RPMI incubated at 37°C for 30-45 mins in a shaker incubator before filtering through 70 µm filters and lysing RBCs with ACK lysis buffer ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
bioRxiv - Neuroscience 2022Quote: ... The SNc regions were dissected out from each slice and incubated for 20–30 min at 36–37℃ in oxygenated high-glucose HEPES-buffered saline containing 4–8 U/ml papain (Worthington Biochemical Corp., USA, LK0003176). After enzymatic digestion ...
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bioRxiv - Neuroscience 2024Quote: ... ∼20 hCOs were chopped into small pieces using a #10 blade and treated with 30 U/ml Papain (Worthington Biochemical, CAS: 9001-73-4) for 60-90 minutes at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... and digested at 37°C for 20 min with Collagenases-I and - IV (10 U/mL, 400 U/mL final, Worthington, Cat# LS004194, and LS004184) and DNase I (30 U/mL ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004194, 100 mg, $42.00 Ask -
bioRxiv - Physiology 2021Quote: ... The proteins were digested for 5 hours with Lys-C (Wako) at room temperature and overnight with trypsin (Worthington). Samples were acidified and diluted with TFA to a final concentration of 1% TFA before being loaded on the Sep-Pak Light C18 cartridges (Waters) ...
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bioRxiv - Neuroscience 2021Quote: ... pH adjusted to 7.6] and mechanically dissociated to single cells after adding ovomucoid (Worthington, 0.7 mg per 5 mice) and DNAse (Worthington ...
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bioRxiv - Cancer Biology 2021Quote: ... and resuspended in Dispase II (5 mg/mL; StemCell 7913) and DNase I (100 μg/mL; Worthington Biochemical LS002006) for 5 minutes ...
Product cited:
Chromatographically purified. A lyophilized powder with glycine as a stabilizer.Cat# LS002006, 20 mg, $96.00 Ask -
bioRxiv - Cell Biology 2020Quote: ... lungs were perfused with 5 ml PBS and then digested with 4 U/ml elastase (Worthington Biochemical Corporation, NJ) and 100 U/ml DNase I (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... cells were centrifuged for 5 minutes and the pellet was resuspended with DNAase/ovomucoid inhibitor according to manufacturer’s protocol (Worthington). Cells were then centrifuged and resuspended on ice in Leibovit’z L-15 medium supplemented with 0.1% of bovine serum albumin ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryonic single cell suspensions from were obtained by performing eight rounds of enzymatic digestion (5 minutes each) with Collagenase type II (0.7mg/ml Worthington) at 37°C under agitation ...
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bioRxiv - Immunology 2023Quote: ... 15 mL of pre-warmed digestion solution (1X HBSS with Ca+ and Mg, 5% FBS, 50 U/ml Dispase II [Millipore-Sigma, cat # 4942078001], 0.5 mg/ml DNAse I [Worthington Biochemical ...
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Molecular Insights into the Recognition of Acetylated Histone Modifications by the BRPF2 BromodomainbioRxiv - Biochemistry 2022Quote: ... The nuclei suspension was preincubated at 37°C for 5 min and followed by micrococcal nuclease (Worthington, cat# LS004798) digestion was carried out by following the manufacturer’s protocol ...
Product cited:
Chromatographically purified to be essentially homogeneous chromatographically and...Cat# LS004798, 45 ku, $211.00 Ask -
bioRxiv - Physiology 2023Quote: ... The muscle layer was cut into pieces and incubated in 5 ml of digestive solution containing 1.5 mg collagenase (Worthington), 1 mg trypsin (Sigma ...
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bioRxiv - Immunology 2023Quote: ... The remaining tissue was then incubated in 5 mL of digest solution [200 U/mL Collagenase IV (Worthington, LS004189) and 400 U/mL DNase I (Worthington ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004189, 5 gm, $900.00 Ask -
bioRxiv - Immunology 2024Quote: ... cut into small ∼1mm3 pieces and enzymatically digested in DMEM containing 5 mg/mL type II collagenase (Worthington, # LS004202) and 0.2 mg/mL DNase I (Roche ...
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bioRxiv - Physiology 2020Quote: ... oocytes contained in 1-3 mL of ovary lobes extracted via survival surgery were isolated by enzymatic digestion with collagenase type II (Worthington Biochemical Corp., NJ, USA) under mechanical agitation ...
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bioRxiv - Cell Biology 2021Quote: ... Each tissue was cut and minced with scissors and then placed in 15 ml conical tubes containing digestion buffer (2 ml DPBS and Collagenase II at 3 mg/ml; Worthington Biochemical, Lakewood, NJ, USA) for 40 min of incubation at 37°C with gentle shaking at 100 rpm ...
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bioRxiv - Cancer Biology 2024Quote: The ovarian cancer tissues were incubated in DMEM high glucose culture medium supplemented with 3 mg/ml collagenase D (Worthington-biochem, Lakewood, NJ, USA) at 37°C for 45 min ...
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bioRxiv - Neuroscience 2023Quote: Both retinas of three Csf1rGFP mice were dissected at different timepoints (Day 1, 3, and 7) and incubated with papain (Worthington Biochemical, Freehold, NJ, USA) for 15 min as previously described (26) ...
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bioRxiv - Cancer Biology 2021Quote: ... 100-200 mg of tumor bearing lung tissue was then thoroughly minced with Noyes scissors, prior to incubation with digestion buffer (HBSS supplemented with 5% HI FBS, 125 U/mL collagenase IV (Worthington) and DNase (Roche ...
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bioRxiv - Neuroscience 2021Quote: ... Prepontine regions from 5-6 dpf fish were dissected into Evans buffer and dissociated in 1 mg/ml neutral protease (Worthington) in Evans buffer for 30 min at room temperature with gentle shaking ...
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bioRxiv - Molecular Biology 2020Quote: ... and 5 mM MgCl2) and LC-MS/MS was performed by on-bead digestion with trypsin (TPCK-treated, Worthington Biochemical).
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Harvest and Culture of Porcine Adipose-Derived Mesenchymal Stem Cells for Autologous TransplantationbioRxiv - Cell Biology 2020Quote: ... (Fat Processing Video) Phosphate-buffered saline (PBS; 100 mL) was mixed with 75 mg (±5 mg) of type A collagenase (Worthington Biochemical Corporation ...
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bioRxiv - Immunology 2020Quote: ... Approximately 1cm2 of skin was digested overnight in 5%CO2 at 37°C with 3ml of digestion mix containing 0.8mg/ml Collagenase Type 4 (Worthington; #LS004186) and 0.02mg/ml DNase (Sigma-Aldrich ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004186, 100 mg, $42.00 Ask -
bioRxiv - Cancer Biology 2022Quote: ... Tumor pieces were chopped and incubated 5 min at 37°C in a HBSS-papain based lysis buffer (Worthington PAP) containing DNAse (0.01% ...
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bioRxiv - Physiology 2023Quote: ... Homogenate was digested with liver digestion buffer (5 ml HBSS w/o Ca2+/Mg2+, 200U/ml Collagenase IV (Worthington, USA) (37 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... at 4°C followed by eight rounds of enzymatic digestion (5 minutes each) with Collagenase type II (1mg/ml Worthington) at 37°C ...
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bioRxiv - Physiology 2023Quote: ... Hearts were then perfused for 5 - 10 min with 0.125 mM CaCl2 KRH buffer supplemented with 1mg/mL of type II collagenase (Worthington Biochemical). Ventricles were cut into 0.1 mM CaCl2 solution ...
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bioRxiv - Immunology 2023Quote: ... The right lobes of the lungs were collected in 5 mL of digestion buffer (90% DMEM and 10% PBS and calcium with type 2 collagenase (180 U/ml) (Worthington) and DNase (15μg/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... The material was then transferred to a 5 mL microcentrifuge tube containing 2.5 mL Papain supplemented with 125 uL DNase (Worthington LK003150) and incubated for 30-45 minutes in a 37°C incubator ...
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bioRxiv - Cancer Biology 2024Quote: ... the tissue was enzymatically and mechanically digested for 5-10 minutes at 37°C in HBSS-papain-based lysis buffer (Worthington) containing DNAse (0.01% ...