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Citations for Worthington Biochemical :
151 - 200 of 322 citations for 3 hydroxy 20 oxopregn 5 en 21 yl acetate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... and digested with collagenase II (5 mg/ml in HBSS) (Worthington Biochemical, #LS004176) for 1 hour at 37 □ on shaker at speed of 200 rpm ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Genomics 2020Quote: ... Cortices acquired from multiple sets of male littermates were incubated in activated 20 U/mL Papain/DNase (Worthington) for 15 minutes at 37°C ...
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β-catenin signaling via astrocyte-encoded TCF7L2 regulates neuronal excitability and social behaviorbioRxiv - Neuroscience 2020Quote: Cortices from embryos (E14.5, E17,5) and pups (P7) were dissected and dissociated with papain 20 U/mL (Worthington) for 70 minutes at 34°C ...
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bioRxiv - Developmental Biology 2024Quote: ... Whole striatal tissues including the globus pallidus were digested with 20 units/ml papain (LS003124, Worthington, Lakewood USA) diluted in dissection solution (5 mM MgCl2 and 5 mM HEPES in 1× Hanks’ balanced salt solution ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003124, 25 mg, $40.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... Organoids were dissociated into single cells using a 1:20 mixture of DNAse in PBS (Worthington Biochemical, LK003163) and TrypLE (Thermo Fisher ...
Product cited:
Complete kit as described for product Code: PDS, but without the Earle's Balanced Salt Solution (EBSS).Cat# LK003163, 3 bx, $715.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... hCOs were dissociated into single cells using a 1:20 mixture of DNAse in PBS (Worthington Biochemical, LK003163) and TrypLE (Thermo Fisher ...
Product cited:
Complete kit as described for product Code: PDS, but without the Earle's Balanced Salt Solution (EBSS).Cat# LK003163, 3 bx, $715.00 Ask -
bioRxiv - Cell Biology 2021Quote: ... HBSS was discarded and stromal cells were released by digestion with 3 mg/ml Type I Collagenase (Worthington) in 1 ml HBSS for 10 min twice at 37°C with shaking at 110 rpm ...
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bioRxiv - Developmental Biology 2022Quote: ... the mouse knee was minced into small fragments and digested with 3 mg/ml collagenase type I (Worthington) and 4 mg/ml dispase (Roche ...
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The cellular basis of protease activated receptor type 2 (PAR2) evoked mechanical and affective painbioRxiv - Neuroscience 2020Quote: ... followed by 25 minutes of incubation at 37□C in 3 mg/ml collagenase type 2 (LS004176; Worthington) and 2 mg/ml Dispase II (04942078001 ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Bioengineering 2022Quote: ... vessels were removed from the perfused system and incubated in 3 Kunitz/ml protease-free DNAse (Worthington, LS006343) in 1x enzyme reaction buffer (40 mM Tris-HCl pH 8.0 ...
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bioRxiv - Physiology 2024Quote: ... cut into small fragments and dissociated in 2 mL HBSS containing 3 mg/mL collagenase type II (Worthington), 7.5mg/mL dispase II and 0.25 mg/mL DNase I for 30 minutes at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... The plugs were digested with 1 ml of a solution of 3 mg/ml type I collagenase (Worthington) dissolved in HBSS for 10 min at 37 °C with 110 rpm shaking ...
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bioRxiv - Neuroscience 2021Quote: ... we removed the sheath covering the brain using collagenase (5% in extracellular saline, Worthington Biochemical Corporation Collagenase Type 4 ...
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bioRxiv - Neuroscience 2022Quote: ... that contained 9.5%v/v Ovomucoid Inhibitor and 5%v/v DNase (Worthington Biochemicals). This resuspended solution was incubated at 37°C for 10 minutes and then re-spun at 1500rpm for 5 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... 5 mM MgCl2 and 10 U/mL DNaseI (Worthington; Code: DPRF; Cat. No. LS006333) were then added to the solution ...
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bioRxiv - Genomics 2023Quote: ... Processing buffer (5% FBS in 1XPBS) and DNase I (100 μg/mL, Worthington LS006344) were then added ...
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bioRxiv - Microbiology 2024Quote: ... minced and enzymatically digested in 5 mL of 0.7 mg/mL collagenase IV (Worthington), 50 μg/mL DNAse I (Worthington ...
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bioRxiv - Neuroscience 2020Quote: ... The cut sections are dissociated while partially frozen in 300 μL of 20 units papain (# LK003178, Worthington Biochemical Corporation) in 15 mL Hibernate-E media (Thermo Fisher Scientific ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003178, 5 vi, $98.00 Ask -
bioRxiv - Biochemistry 2020Quote: ... DRGs were digested in 1 mL of Ca2+/Mg2+-free HBSS containing 20 U of papain (Worthington, Lakewood, NJ), 0.35 mg of L-cysteine and 1 μL of saturated NaHCO3 and incubated at 37°C for 10 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... and individually incubated at 37°C for 15-20 minutes in a sterile solution of 45 U papain (Worthington), 0.01% DNase (Sigma) ...
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bioRxiv - Neuroscience 2022Quote: Fresh retinae were dissected before being placed in activated papain/DNase (1:20 solution; Worthington Biochemicals, New Jersey, USA) and incubated at 37°C for 45 minutes on a shaker ...
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bioRxiv - Cancer Biology 2020Quote: ... Micro-dissected regions were dissociated by incubating with papain (20 units/ml) DNase (0.005%) for 30min at 37°C (Worthington). Samples were triturated in HEPES buffered EBSS to fully dissociate the tissue and centrifuged (3 min ...
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bioRxiv - Cell Biology 2020Quote: ... and then they were incubated in 20 mL of DMEM media containing 0.2 % collagenase type II (Worthington Biochemical Corporation) and 2.5 U/mL dispase (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... tumors were mechanically dissociated and subjected to enzymatic digestion for 20 min with 200 U/ml collagenase IV (Worthington), 5 mM CaCl2 ...
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bioRxiv - Neuroscience 2023Quote: ... The collected tissue was incubated with continuous shaking at 37°C for 45 min in 1 ml of papain solution (20 U/ml) and 0.2 ml DNase (both from Worthington) prepared in isolation buffer ...
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bioRxiv - Neuroscience 2022Quote: ... The pieces were dissociated first using the ice-cold oxygenated dissection buffer added papain (20 units/ml, Worthington, LS003126), 0.067 mM 2-mercaptoethanol (Sigma ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003126, 100 mg, $88.00 Ask -
bioRxiv - Physiology 2024Quote: ... The cortical tissue from each brain was pooled and digested in papain solution (20 U/mL Cat# LS003126, Worthington) and then treated with DNase I (Cat# LS006563 ...
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bioRxiv - Neuroscience 2024Quote: ... and digested for 30 min using 2mL of HEPES-buffered HBSS containing 20 U/ml papain (Worthington Biochem., USA), 1mM EDTA (PanReac AppliChem ...
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bioRxiv - Neuroscience 2024Quote: ... Hippocampal tissue isolated from each hemisphere was pooled and digested in papain solution (20 U/mL Cat# LS003126, Worthington) and then treated with DNase I (Cat# LS006563 ...
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bioRxiv - Cell Biology 2024Quote: Kidneys were minced and digested for 20 minutes in 1 mg/mL Collagenase A (Worthington Biochemical, Lakewood, New Jersey). Isolates were pushed through 50 mL conical tube sterile 100 µm cell strainers and washed with cold 35 mL HBSS (Gibco ...
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bioRxiv - Developmental Biology 2021Quote: ... 3 sutures each from litter 2) were cut into small fragments in HBBS and digested using Collagenase IV (Worthington, USA ...
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bioRxiv - Neuroscience 2021Quote: ... Thermo Fisher, 31966-021 with 3.6 mg/ml glucose, Carl Roth, NH06.3, 3 mg/ml Collagenase type IV, Worthington, LS004186 ...
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bioRxiv - Neuroscience 2021Quote: Differentiated human neural cultures (3 months old) were dissociated into single-cells suspension using Papain Dissociation System (Worthington #LK003150), passed through 70µm and 40µm cell strainers (Falcon #07-201-431 and #07-201-430) ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Bioengineering 2021Quote: ... The solution was replaced with 200 μl trehalose-HEPES-ACSF containing 3 mg/ml ovomucoid inhibitor (OI-BSA, Worthington) and 1 μl DNase I ...
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bioRxiv - Neuroscience 2020Quote: ... and resuspended in 3 ml HA –Ca containing 10% v/v ovomucoid (Worthington, resuspended in 32 ml HA –Ca). Cell debris was removed using discontinuous density gradient containing 3 ml tissue mixture on top of 5 ml ovomucoid solution ...
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bioRxiv - Neuroscience 2021Quote: ... Dissected brains were chilled on ice and minced in digestion media containing with 0.2% collagenase type 3 (LS004182, Worthington) and 3 U/mL dispase (LS02104 ...
Product cited:
Lower in secondary proteolytic contaminant activities but with typical collagenase activity. ...Cat# LS004182, 1 gm, $215.00 Ask -
bioRxiv - Developmental Biology 2022Quote: ... MM from E11.5 kidneys from CD-1 mice were dissociated with Collagenase Type 3 (Worthington Biochemical Corporation; Cat #LS004180) and DNase I (New England Biolabs ...
Product cited:
Lower in secondary proteolytic contaminant activities but with typical collagenase activity. ...Cat# LS004180, 100 mg, $42.00 Ask -
bioRxiv - Developmental Biology 2022Quote: ... and digested with gentle rocking for 1 h at 37°C in 3 mg/mL collagenase II (Worthington, LS004176) diluted in HBSS containing 5% fetal bovine serum (FBS) ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Immunology 2020Quote: ... and skin was minced finely with dissection scissors and mixed in a 6-well plate with 3 ml of digestion buffer consisting of 0.8 mg/ml Collagenase Type 4 (4188; Worthington), 0.02 mg/ml DNAse (DN25-1G ...
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bioRxiv - Neuroscience 2024Quote: ... followed by incubation at 37° C for 25 minutes in 3 mg/mL collagenase type 2 (LS004174, Worthington Biochemical) and 2 mg/mL Dispase II (4942078001 ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004174, 100 mg, $42.00 Ask -
bioRxiv - Cancer Biology 2023Quote: ... The minced tumors were digested with 5ml to 10ml of 1 mg/ml Collagenase 3 (Worthington cat. no. LS004183) plus 0.5mg/ml Hyaluronidase (Sigma-Aldrich H3884 ...
Product cited:
Lower in secondary proteolytic contaminant activities but with typical collagenase activity. ...Cat# LS004183, 5 gm, $900.00 Ask -
bioRxiv - Cancer Biology 2023Quote: ... and spleen (n = 3; 12-16 PCW) samples were diced and digested with 1.6mg/mL collagenase type IV (Worthington) in RPMI (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2024Quote: We extracted cells from the central body of freshly dissected menisci from 3-week-old WT and Col5a1+/− mice by 0.2% collagenase (CLS-2, Worthington) digestion for 5 h at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... the tendons were minced to 1mm3 pieces and digested with 3 mg/ml collagenase I (Worthington biochemical, cat# LS004194) in DMEM (Gibco ...
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DDK regulates replication initiation by controlling the multiplicity of Cdc45-GINS binding to Mcm2-7bioRxiv - Cell Biology 2020Quote: ... Proteins were released from the DNA by incubation with 5 U of DNA I (Worthington) in 10 mL of 25 mM HEPES-KOH (pH 7.6) ...
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bioRxiv - Microbiology 2022Quote: Lungs were collected and digested at 37 with 5 mg/mL of collagenase I (Worthington) and 1 mg/mL of DNase I (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... The dissociated cells were layered on top of 5 ml of ovomucoid inhibitor solution (Worthington) and harvested by centrifugation (70 x g for 6 min) ...
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bioRxiv - Genetics 2023Quote: ... 5 µg/mL of Oxyrase (Oxyrase Inc.) and 150 U/mL Catalase (Worthington Bio Crop), or on 5% Sheep’s blood agar plates at 370C in a 5% CO2 atmosphere ...
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bioRxiv - Neuroscience 2023Quote: ... and the pellet was resuspended in 5 mL dissociation media containing 165U of Papain (Worthington) and DNAse type I (40 µg/mL ...
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bioRxiv - Immunology 2024Quote: ... before enzymatic digestion in 0.5mL DMEM containing 5 mg/mL type II collagenase (Worthington, # LS004202) and 0.2 mg/mL DNase I (Roche ...