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Citations for Worthington Biochemical :
151 - 200 of 608 citations for 1 6 Chloropyridazin 3 yl piperidine 4 carboxamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... the mouse knee was minced into small fragments and digested with 3 mg/ml collagenase type I (Worthington) and 4 mg/ml dispase (Roche ...
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The cellular basis of protease activated receptor type 2 (PAR2) evoked mechanical and affective painbioRxiv - Neuroscience 2020Quote: ... followed by 25 minutes of incubation at 37□C in 3 mg/ml collagenase type 2 (LS004176; Worthington) and 2 mg/ml Dispase II (04942078001 ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Bioengineering 2022Quote: ... vessels were removed from the perfused system and incubated in 3 Kunitz/ml protease-free DNAse (Worthington, LS006343) in 1x enzyme reaction buffer (40 mM Tris-HCl pH 8.0 ...
Product cited:
Molecular Biology Grade. Chromatographically purified to remove RNase and protease. ...Cat# LS006343, 50 ku, $640.00 Ask -
bioRxiv - Physiology 2024Quote: ... cut into small fragments and dissociated in 2 mL HBSS containing 3 mg/mL collagenase type II (Worthington), 7.5mg/mL dispase II and 0.25 mg/mL DNase I for 30 minutes at 37°C ...
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bioRxiv - Physiology 2020Quote: ... Tissue chunks were transferred into a digestion device and then subjected to sequential (6 steps) enzymatic digestion (at 37°C) using collagenase type V (Worthington Biochemical Corporation, Lakewood, NJ) 250 U/mL and protease type XXIV (Sigma-Aldrich Co. ...
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bioRxiv - Developmental Biology 2024Quote: Six corneas from 2 female and 1 male 129S6/SvEvTac mice of each age group (2, 6, and 11-month-old) were dissected and pooled in 600 µL of papain solution (CAT LK003178; Worthington Biochemical Corp., Lakewood, NJ) and incubated for 1.75 hours at 37 °C ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003178, 5 vi, $98.00 Ask -
bioRxiv - Genetics 2022Quote: ... blood was removed by perfusion and the heart was digested with collagenases 2 and 4 (Worthington) and Protease XIV (Sigma) ...
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bioRxiv - Physiology 2021Quote: ... The oocytes were manually defolliculated after treating them with collagenase type 4 (Worthington Biochemical, Lakewood, NJ). Stage V and VI oocytes were selected ...
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bioRxiv - Developmental Biology 2021Quote: ... 3 sutures each from litter 2) were cut into small fragments in HBBS and digested using Collagenase IV (Worthington, USA ...
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bioRxiv - Neuroscience 2021Quote: ... Thermo Fisher, 31966-021 with 3.6 mg/ml glucose, Carl Roth, NH06.3, 3 mg/ml Collagenase type IV, Worthington, LS004186 ...
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bioRxiv - Neuroscience 2021Quote: Differentiated human neural cultures (3 months old) were dissociated into single-cells suspension using Papain Dissociation System (Worthington #LK003150), passed through 70µm and 40µm cell strainers (Falcon #07-201-431 and #07-201-430) ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Bioengineering 2021Quote: ... The solution was replaced with 200 μl trehalose-HEPES-ACSF containing 3 mg/ml ovomucoid inhibitor (OI-BSA, Worthington) and 1 μl DNase I ...
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bioRxiv - Neuroscience 2020Quote: ... and resuspended in 3 ml HA –Ca containing 10% v/v ovomucoid (Worthington, resuspended in 32 ml HA –Ca). Cell debris was removed using discontinuous density gradient containing 3 ml tissue mixture on top of 5 ml ovomucoid solution ...
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bioRxiv - Neuroscience 2021Quote: ... Dissected brains were chilled on ice and minced in digestion media containing with 0.2% collagenase type 3 (LS004182, Worthington) and 3 U/mL dispase (LS02104 ...
Product cited:
Lower in secondary proteolytic contaminant activities but with typical collagenase activity. ...Cat# LS004182, 1 gm, $215.00 Ask -
bioRxiv - Neuroscience 2024Quote: ... followed by incubation at 37° C for 25 minutes in 3 mg/mL collagenase type 2 (LS004174, Worthington Biochemical) and 2 mg/mL Dispase II (4942078001 ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004174, 100 mg, $42.00 Ask -
bioRxiv - Cancer Biology 2023Quote: ... and spleen (n = 3; 12-16 PCW) samples were diced and digested with 1.6mg/mL collagenase type IV (Worthington) in RPMI (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2024Quote: We extracted cells from the central body of freshly dissected menisci from 3-week-old WT and Col5a1+/− mice by 0.2% collagenase (CLS-2, Worthington) digestion for 5 h at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... the tendons were minced to 1mm3 pieces and digested with 3 mg/ml collagenase I (Worthington biochemical, cat# LS004194) in DMEM (Gibco ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were pelleted 200g 5min at 4°C and resuspended in 200μl aCSF with 2.5% DNaseI (Worthington Papain system ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were pelted 200g 5min at 4°C and resuspended in 200μl aCSF with 2.5% DnaseI (Worthington Papain system ...
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bioRxiv - Immunology 2024Quote: ... for RNA extraction or into a digestion buffer containing 1.6 mg/ml collagenase type 4 (Worthington LS004189) and 100 µg/ml DNase I (Roche 11284932001 ...
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bioRxiv - Cancer Biology 2020Quote: Spleens were collected into RPMI 1640 containing 2% fetal calf serum (FCS) and dissociated with a scalpel and type 3 collagenase (Worthington) and DNAse I ...
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bioRxiv - Developmental Biology 2021Quote: ... and 10 pooled coronal sutures were rinsed in PBS and enzymatically dissociated with a final concentration of 3 mg/mL of Collagenase II (Worthington) and 4 units/mL of Dispase (Corning ...
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bioRxiv - Neuroscience 2022Quote: ... Tissues were then incubated at 37°C for 3 minutes in a solution of HBSS/0.2% glucose with 10 mg/ml trypsin (Worthington Biochemical) and 1 mg/ml Deoxyribonuclease I (Worthington Biochemical) ...
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bioRxiv - Genomics 2024Quote: ... we replaced the medium with 2 mL of digestive enzyme mixture I (3 mg/mL collagenase I (Worthington, cat. LS004196), 3 mg/mL collagenase II (Worthington ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004196, 1 gm, $215.00 Ask -
bioRxiv - Immunology 2024Quote: ... Spleens and non-draining lymph nodes were digested in 3 mL of 1.6 mg/mL collagenase Type IV (Worthington Biochemical), 0.1 mg/mL DNase I (Thomas Scientific ...
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bioRxiv - Immunology 2023Quote: ... A 2 cm2 patch of skin was minced and incubated in 3 mg/mL collagenase Type IV (Worthington Biochemical Corporation) and 2 mg/mL DNase (Sigma-Aldrich ...
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bioRxiv - Genetics 2022Quote: ... cortices and hippocampi acquired from 12 male mice from 3 separate litters were combined and dissociated using activated 20 U/mL Papain/DNase (Worthington) diluted in Hibernate A (Gibco ...
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bioRxiv - Neuroscience 2024Quote: ... 3 mM CNQX) and single cell suspensions were acquired with an entire mouse cortex using a papain dissociation kit (Worthington Biochemical ...
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bioRxiv - Neuroscience 2024Quote: ... samples were pre-cut with small scissors and diluted and digested in 2:3 stock solution of CollagenaseI (10 U/ml, Worthington), Collagenase IV (400 U/ml ...
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bioRxiv - Cancer Biology 2021Quote: ... Right lung lobes were minced and digested with 5 mL digestion buffer: Collagenase 4 (200U/mL, Worthington Biochemicals), DNase I (100 ug/mL ...
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bioRxiv - Immunology 2021Quote: ... Cells were then DNAse treated with 4 ml dissagregation media (DMEM, 10% FBS, 100 μg/ml DNAse (Worthington)) for 20 min at 37 °C ...
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bioRxiv - Bioengineering 2024Quote: ... and they were further digested for 30 min in 4 mg/ml collagenase II (Worthington, cat. No. LS004176) and 4.66 mg/ml dispase II (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... enzymatic digestion was achieved by 3 min of perfusion with calcium-free digestion buffer (400 units/mL of collagenase II in perfusion buffer; Worthington LS004177) followed by 12 min of perfusion with digestion buffer containing 50 μM CaCl2 ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004177, 5 gm, $900.00 Ask -
bioRxiv - Cell Biology 2023Quote: ... The tissue was then washed with PBS and digested for 10 min at 37°C in 3 mg/ml collagenase II (Worthington, LS004176) in HBSS supplemented with 5% FBS and 10 mM HEPES ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Molecular Biology 2024Quote: ... Excised hearts were cannulated to the Langendorff retrograde perfusion system through the aorta and perfused with Base solution (Tyrode’s solution with 10 mM taurine and 12 mM 2,3-butanedione monoxime) and then digested with prewarmed Digestion buffer (Base solution with 180 U/mL Collagenase Type II (Worthington, LS004177) and 25 μM CaCl2 ...
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bioRxiv - Immunology 2022Quote: ... biopsies were minced finely with dissection scissors and mixed with 3mL of digestion buffer (0.8mg/mL collagenase type 4 (Worthington) and 0.02mg/mL DNase I (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... and 1 mL of Ca2+/Mg2+-free HBSS containing 4 mg of collagenase type II and 1.25 mg of Dispase type II (Worthington) was added and incubated at 37°C for 15 minutes ...
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Pulmonary infection interrupts acute cutaneous wound healing through disruption of chemokine signalsbioRxiv - Immunology 2020Quote: ... The tissue was incubated for 45 minutes at 37° C in DMEM containing type 4 collagenase (Worthington Biochemical Corporation) and DNAse I (Sigma-Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... plates were coated overnight at 4°C with 15μl purified proteins at optimized concentrations in 0.1M Sodium Bicarbonate: DNA (Worthington, LS002105) at 5μg/mL ...
Product cited:
Highly polymerized; hyperchromicity ≥27%. A substrate for deoxyribonuclease assays. Prepared...Cat# LS002105, 100 mg, $35.00 Ask -
bioRxiv - Cell Biology 2020Quote: ... lungs were perfused with 5 ml PBS and then digested with 4 U/ml elastase (Worthington Biochemical Corporation, NJ) and 100 U/ml DNase I (Sigma ...
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bioRxiv - Immunology 2023Quote: ... Cells were then DNase treated with 4 ml disaggregation media (DMEM, 10% FBS, 100 μg/ml DNASE I (Worthington)) for 20 min at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... and skin was cut into small pieces and mixed with digestion buffer containing 0.8 mg/ml Collagenase Type 4 (4188; Worthington), 0.02 mg/ml DNAse (DN25-1G ...
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bioRxiv - Neuroscience 2020Quote: ... Acute bilateral fastigial nuclei were quickly excised with a sharp knife from 2 or 3 cerebellar slices and were then enzymatically digested with cysteine (2 mM)-supplemented papain (40 U/ml, Worthington, Lakewood, NJ) and chondroitinase-ABC (1 U/ml ...
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bioRxiv - Neuroscience 2023Quote: ... Cell pellet was collected by centrifuged at 300 g for 5 min and then resuspended in 3 mL Ovomucoid protease inhibitor with FBS (Inhibitor-BSA Vial [LK003182], Worthington, Lakewood, NJ). To remove the debris ...
Product cited:
Ovomucoid protease inhibitor and bovine serum albumin which is 0.22 micron filtered and...Cat# LK003182, 1 vi, $86.00 Ask -
bioRxiv - Cell Biology 2024Quote: ... the RPE cells were washed at least 3 times with PBS to remove the dead cells and dissociated using Papain (Worthington Biochemical Corporation) for 30mins-1h at 37 °C following the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2024Quote: ... Dissociation medium was aspirated until 1 mL remained and an activated papain solution (1:1 papain [Worthington-Biochem] with 13.6 mM Cysteine-HCL ...
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bioRxiv - Developmental Biology 2021Quote: ... The dissected hypothalamuses were further divided into 2-4 pieces before cell dissociation for single cell isolation using Papain Dissociation System (cat no. LK003150, Worthington Biochemical Corporation ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Bioengineering 2020Quote: ... acellular PCL and GA or GP crosslinked dMEP scaffolds (n = 4/group) were digested in 2 mg/mL collagenase type 2 solution (Worthington) for 24 h at 37°C ...
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bioRxiv - Bioengineering 2020Quote: ... Cell suspensions were harvested by mechanical dissection and Collagenase IV Cocktail digestion (formulated as 7.4 kU Collagenase Type 4, 53 kU Deoxyribonuclease I, 20 mg Soybean Trypsin Inhibitor in 10 mL cocktail; Worthington Biochemical). The suspensions were passed through 40 μm cell strainers to ensure singularity ...