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Citations for Worthington Biochemical :
201 - 250 of 608 citations for 1 6 Chloropyridazin 3 yl piperidine 4 carboxamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... Cell suspensions were harvested by mechanical dissection and Collagenase IV Cocktail digestion (formulated as 7.4 kU Collagenase Type 4, 53 kU Deoxyribonuclease I, 20 mg Soybean Trypsin Inhibitor in 10 mL cocktail; Worthington Biochemical). The suspensions were passed through 40 μm cell strainers to ensure singularity ...
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bioRxiv - Immunology 2020Quote: ... Approximately 1cm2 of skin was digested overnight in 5%CO2 at 37°C with 3ml of digestion mix containing 0.8mg/ml Collagenase Type 4 (Worthington; #LS004186) and 0.02mg/ml DNase (Sigma-Aldrich ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004186, 100 mg, $42.00 Ask -
bioRxiv - Developmental Biology 2022Quote: ... at 4°C followed by eight rounds of enzymatic digestion (5 minutes each) with Collagenase type II (1mg/ml Worthington) at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... and enzymatically digested at 37°C for 80 min in dispase type II (4.7 mg/ml) plus collagenase type II (4 mg/ml) (Worthington Biochemical), as described previously [62] ...
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bioRxiv - Developmental Biology 2023Quote: ... washed with PBS and dissociated into single cells using collagenase type 4 enzyme at 200Units/ml (Worthington, Lakewood, NJ, US) for 10min at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... The RNA was then digested for 2 hr at 37 °C in 1/10 volume of 1 M ammonium bicarbonate with snake venom phosphodiesterase (1.2×10−4 U/μg RNA) and bacterial alkaline phosphatase (0.1U/ μg RNA) (Worthington Biochemical corporation). Analysis was performed on a TSQ Quantiva Triple Quadrupole (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... supplemented with 0.3 mg/ml Collagenase Type 1 (Worthington, CLS-1) and 10 mg/ml DNase I (Sigma ...
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bioRxiv - Genetics 2021Quote: Left hind legs of P7 mice were isolated from one WT and one Col12a1-/- mouse and digested in 4 mg/mL collagenase II (Worthington, USA) in DMEM/F-12 (supplied with 10% FBS ...
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bioRxiv - Immunology 2020Quote: ... and both fragments were separately digested in a petri-dish at 37°C 5% CO2 in RF-10 media with 1.6 mg/ml type IV collagenase (Worthington, CLS-4) overnight (12 hours) ...
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bioRxiv - Cancer Biology 2022Quote: ... tumor samples were transferred into a 5mL Eppendorf tube containing 4.5mL cold enzymatic dissociation mix (100µg/mL Collagenase Type 4 (Worthington Biochemical Corporation #LS004186) and 10 µg/mL DNAse I (StemCell Technologies #07900 ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004186, 100 mg, $42.00 Ask -
bioRxiv - Biochemistry 2024Quote: ... epidydimal fat from 4-month-old male DIO C57BL/6J mice was dissected out and dissociated using 2 mg/ml Collagenase B (Worthington, CLSAFB) and 1 mg/ml soybean trypsin inhibitor (Worthington ...
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bioRxiv - Genetics 2023Quote: ... and perfused with a type 2 collagenase digestion buffer (2.4 mg/ml concentration) at a flow rate of 4 ml/min (Worthington Cat# LS004176). The large blood vessels and the atria were excised from the ventricles and minced in a stop buffer containing 10% calf serum ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Synthetic Biology 2024Quote: ... and both fragments were separately digested in a petri dish at 37°C 5% CO2 in RF-10 media with 1.6 mg/ml type IV collagenase (Worthington, CLS-4) for 12 hours ...
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bioRxiv - Cell Biology 2021Quote: ... Each tissue was cut and minced with scissors and then placed in 15 ml conical tubes containing digestion buffer (2 ml DPBS and Collagenase II at 3 mg/ml; Worthington Biochemical, Lakewood, NJ, USA) for 40 min of incubation at 37°C with gentle shaking at 100 rpm ...
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bioRxiv - Cancer Biology 2024Quote: The ovarian cancer tissues were incubated in DMEM high glucose culture medium supplemented with 3 mg/ml collagenase D (Worthington-biochem, Lakewood, NJ, USA) at 37°C for 45 min ...
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bioRxiv - Neuroscience 2020Quote: ... prepared using 1:1 albumin-ovomucoid solution (10mg/ml of each) (Worthington, Cat# OI ...
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bioRxiv - Cancer Biology 2024Quote: ... containing 1 mg/ml each of Collagenase Type 1 (Worthington Biochemical LS004216), Type 2 (Worthington Biochemical LS004204) ...
Product cited:
Collagenase, Type 1 (Code: CLS-1), which is filtered through a 0.22 micron membrane and...Cat# LS004216, 5x50 mg, $200.00 Ask -
bioRxiv - Genomics 2023Quote: ... for 1 hour (Patient #1) or 3.7mg/mL collagenase II (Worthington, LS004177) for 90 minutes followed by 6mg/mL dispase (Gibco ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004177, 5 gm, $900.00 Ask -
bioRxiv - Neuroscience 2020Quote: Brain samples were minced with scissors and then incubated at 37 °C on a roller for 30 min in digest buffer containing 1.25 mg/mL Collagenase Type 4 (Worthington, Lakewood, NJ, USA) and 100 U/mL DNAseI (Sigma ...
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bioRxiv - Developmental Biology 2023Quote: 30 hindbrains of st.18 chick embryos were harvested and disassociated into single cells using collagenase type 4 (200 Units\ml, Worthington 47B9407, USA). Cells were then centrifuged at 600 g for 10 min ...
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bioRxiv - Developmental Biology 2023Quote: ... 60 hindbrains of st.18 chick embryos were harvested and disassociated into single cells using collagenase type 4 (200 Units\ml, Worthington 47B9407, USA). Cells were then centrifuged at 600 g for 10 min ...
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bioRxiv - Bioengineering 2024Quote: ... The DRGs from 4 pups were digested for 30 min at 37°C using 13.33 U/ml papain (Worthington, cat. No. LS003126). The DRGs were then centrifuged for 1 min at 500g ...
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bioRxiv - Neuroscience 2023Quote: ... and 1% DNase (Worthington) at 37°C for 6 minutes at which point DMEM (Gibco ...
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bioRxiv - Biochemistry 2024Quote: ... Lungs from 4 mice were minced into ∼1 mm pieces using curved scissors and digested with 1 mg/ml collagenase type 1 (Worthington, cat# LS004194) and 1X dispase (Corning ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004194, 100 mg, $42.00 Ask -
bioRxiv - Immunology 2023Quote: ... and then digested for 30 minutes under gentle agitation at 37°C with 7 mL of 4 mg/mL of type III collagenase (Worthington Biochemical, Lakewood, NJ) and 0.05 mg/mL of DNase I (Sigma ...
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bioRxiv - Genetics 2020Quote: ... 1 mg ml-1 soybean trypsin inhibitor (#LS003570, all purchased from Worthington Biochemical Corporation), and 1% penicillin/streptomycin in HBSS for 10 min at 37°C with gentle shaking ...
Product cited:
Chromatographically purified as described by Frattali and Steiner, Biochem., 7, 521 (1968). A...Cat# LS003570, 100 mg, $74.00 Ask -
bioRxiv - Neuroscience 2020Quote: ... papain (1 mg/ml, Worthington), ethylenediaminetetraacetic acid (EDTA ...
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bioRxiv - Immunology 2023Quote: ... 1 mg/ml hyaluronidase (Worthington), 0.1 mg/ml DNase I (Roche ...
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bioRxiv - Neuroscience 2022Quote: ... The SNc regions were dissected out from each slice and incubated for 20–30 min at 36–37℃ in oxygenated high-glucose HEPES-buffered saline containing 4–8 U/ml papain (Worthington Biochemical Corp., USA, LK0003176). After enzymatic digestion ...
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bioRxiv - Neuroscience 2024Quote: ... ∼20 hCOs were chopped into small pieces using a #10 blade and treated with 30 U/ml Papain (Worthington Biochemical, CAS: 9001-73-4) for 60-90 minutes at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... These chunks were immersed in 5 ml of pre-warmed enzyme solution (2 mg/mL STEMzyme I, 4 µg/mL DNAse I obtained from Worthington Biochemical #LS004107 and #LS002139) in Hanks’ Balanced Salt Solution (HBSS ...
Product cited:
A specialized combination of Animal Free Clostridium histolyticum collagenase (Code: CLSAFB) and...Cat# LS004107, 5x50 mg, $495.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... This solution was then replaced with 1mg/ml collagenase 1 (Worthington Biochemical Corporation CLS-1) in 0.1M PBS and 20mM HEPES for 60-90 min at 37°C followed by 0.05% Trypsin-EDTA (Sigma T9201 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were spun down and supernatant was removed before the addition of 1 ml Dispase-mUE (Dispase II [7 mg ml-1 DMEM Ham’s F12], Neutral Protease, Worthington NPRO2; and 10 μg μL-1 DNase). Cells were aspirated in the solution gently 40 times using a 1 ml pipette tip ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 ml Trypsin (1 g/ml)-DNase (100 mg/ml) solution (Worthington Biochemical, 3703 and 2139) was added to the pellet for 1.5 min at 37°C without disturbing the pellet ...
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bioRxiv - Microbiology 2023Quote: ... and 1 μg/ml L-1-tosylamido-2-phenylethyl chloromethyl ketone (TPCK)-treated trypsin (Worthington Biochemicals). Plaque forming units (PFU ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mg/ml trypsin inhibitor (Worthington), 2 % (v/v ...
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bioRxiv - Immunology 2022Quote: ... dispase (0.1 U ml−1; Worthington) and 10% FBS ...
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bioRxiv - Physiology 2022Quote: ... 2.5mg/ml collagenase Type 1 (Worthington) made up in low Ca2+/low Mg2+ HBSS ...
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bioRxiv - Microbiology 2021Quote: ... 30 mg ml-1 DNase (Worthington), and 1 mM MgCl2 ...
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bioRxiv - Cancer Biology 2023Quote: ... and Deoxyribonuclease 1 (Cat: LS002139, Worthington). Next ...
Product cited:
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bioRxiv - Developmental Biology 2023Quote: ... 1 U/mL of dispase (Worthington) and 5uM of Ca2+(Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2022Quote: ... and 1:1000 DNase (Worthington Biochemical). 0.02% TritonX-100 (v/v ...
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bioRxiv - Immunology 2024Quote: ... dispase (0.1 U ml−1; Worthington) and 10% FBS ...
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bioRxiv - Neuroscience 2024Quote: ... and 1:100 DNAse (Worthington, LS002007) was added to quench ...
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bioRxiv - Developmental Biology 2020Quote: ... with Papain added to 1 mg/ml (1:10 dilution of 10 mg/ml Papain solution; Worthington)) ...
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bioRxiv - Physiology 2024Quote: ... and digested for 45 minutes using a cocktail containing type 1 collagenase (1.5 mg ml−1; Worthington Biochemical), dispase II (2.5 U ml−1 ...
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bioRxiv - Cell Biology 2024Quote: Protein digestion was conducted by adding trypsin (Worthington; from a 1 mg/mL stock in 1 mM HCl) at a ratio of 1:100 ...
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bioRxiv - Immunology 2022Quote: ... 1 mg/ml Type II collagenase (Worthington), and 50 U/ml Deoxyribonuclease I (Worthington ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mg/mL type 2 collagenase (Worthington), 10 µg/mL gentamycin (Teknova ...
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bioRxiv - Microbiology 2020Quote: ... 1 mg/mL chymotrypsin (Worthington Biochemical Corporation), 1 mg/mL trypsin (Sigma-Aldrich) ...