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Citations for Worthington Biochemical :
1351 - 1400 of 1494 citations for Pops Hrms Hch Clean Up Spike 13C6 99% 50X Stock 1 Ug Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2020Quote: ... and submerged into a solution of phosphate-buffered saline containing 0.5 mM of calcium chloride (pH 7.4) and 250 units/mL type III collagenase (Worthington, Lakewood, NJ, USA) at 37°C for 1.5 h or for 3h.[16] The collagenase used has substrate specificity to collagens with lower proteolytic activity than other collagenases ...
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bioRxiv - Neuroscience 2019Quote: ... 0.16mM NaOH pH=7.4) that contained 10 U/mL papain and 5mg of L-cysteine in total (Worthington, Lakewood, NJ, USA) for 10 minutes at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... muscles were cut into 5 mm2 pieces with forceps and digested in 2 mg/mL collagenase II (Worthington Chemicals, Lakewood, NJ) for 1h at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: Cells were derived from a minced piece of tumour digested in DMEM supplemented with 200U/ml collagenase type IV (Worthington, USA) at 37 °C for 24 h ...
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bioRxiv - Immunology 2020Quote: ... and both fragments were separately digested in a petri-dish at 37°C 5% CO2 in RF-10 media with 1.6 mg/ml type IV collagenase (Worthington, CLS-4) overnight (12 hours) ...
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bioRxiv - Neuroscience 2020Quote: ... and the supernatant aspirated before the tissue was incubated in a collagenase solution containing 2 mg/mL collagenase type II (CLS2; Worthington Biochemical) in 5 mL 1X HBSS in a 37°C rocking water bath for 10 minutes ...
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bioRxiv - Neuroscience 2022Quote: Organoids collected from different genotypes and time points were dissociated by incubation with a solution of Papain (30U/mL, Worthington LS03126) and DNAseI (3U/uL ...
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bioRxiv - Physiology 2022Quote: ... The hearts were removed and perfused at 37 °C in Langendorff method with nominally Ca2+-free Tyrode’s solution containing 0.5 mg/ml collagenase (Type II; Worthington, Lakewood, NJ, USA) and 0.1 mg/ml protease (type XIV ...
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bioRxiv - Cancer Biology 2022Quote: ... which included a first step of PDX tumor mechanical dissociation with sterile scalpels and then enzymatic dissociation by trypsin (10 mg/ml) and type II collagenase (275 U/mg, Worthington Biochemical). The tube was then placed in a warm 37°C water bath for 60 min ...
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bioRxiv - Neuroscience 2022Quote: ... organoids (single organoids cultured in a 96-well plate or up to 8 organoids cultured in a 6-well plate) were incubated with an enzyme solution containing 10U/ml papain (Worthington, LS003127), 1x EBSS (Sigma-Aldrich ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003127, 1 gm, $595.00 Ask -
bioRxiv - Cell Biology 2022Quote: ... In brief, we dissected the hindlimb muscles from the mouse and digested the minced muscles with collagenase II (800U/mL, (Worthington, LS004177)) in wash medium (F10 ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004177, 5 gm, $900.00 Ask -
bioRxiv - Cancer Biology 2022Quote: ... tumor samples were transferred into a 5mL Eppendorf tube containing 4.5mL cold enzymatic dissociation mix (100µg/mL Collagenase Type 4 (Worthington Biochemical Corporation #LS004186) and 10 µg/mL DNAse I (StemCell Technologies #07900 ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004186, 100 mg, $42.00 Ask -
bioRxiv - Cell Biology 2023Quote: ... The tissue was then washed with PBS and digested for 10 min at 37°C in 3 mg/ml collagenase II (Worthington, LS004176) in HBSS supplemented with 5% FBS and 10 mM HEPES ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... was dissected on ice and minced using two razor blades in a few drops of pre-warmed (37°C) Hibernate A medium containing 20 units/mL papain (Worthington LK003178). Minced tissue was incubated in 3.125 mL pre- warmed papain solution for 10 minutes at 37°C ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003178, 5 vi, $98.00 Ask -
bioRxiv - Molecular Biology 2023Quote: ... Excised hearts were cannulated to the Langendorff retrograde perfusion system through the aorta and perfused with Base solution (Tyrode’s solution with 10 mM taurine and 12 mM 2,3-butanedione monoxime) and then digested with prewarmed Digestion buffer (Base solution with 180 U/mL Collagenase Type II (Worthington, LS004177) and 25 μM CaCl2 ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004177, 5 gm, $900.00 Ask -
bioRxiv - Cancer Biology 2023Quote: ... PBS was supplemented with DNase solution (containing a final concentration of 200U/ml DNase [Worthington], 2.5 mM MgCl2, 500 mM CaCl2). After the third wash ...
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bioRxiv - Cell Biology 2024Quote: ... pieces of the intestine were incubated in DMEM/F12 containing 500 U/ml Collagenase Type IV (Worthington Biochemical Corporation, Lakewood, NJ) for 30 min at 37 °C using a water bath ...
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bioRxiv - Cell Biology 2024Quote: ... whole hearts from 28 wk-old ♂ WT and R14Δ/+ mice were cannulated and perfused with modified Krebs buffer containing 2 mg/ml collagenase (Worthington) for 10 min ...
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bioRxiv - Biochemistry 2024Quote: ... epidydimal fat from 4-month-old male DIO C57BL/6J mice was dissected out and dissociated using 2 mg/ml Collagenase B (Worthington, CLSAFB) and 1 mg/ml soybean trypsin inhibitor (Worthington ...
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bioRxiv - Bioengineering 2024Quote: ... The dissected corneas were then incubated overnight at 37℃ in digestion medium containing 0.5 mg/ml hyaluronidase (Worthington Biochemicals, Lakewood, NJ), 2 mg/ml collagenase (Gibco-ThermoFisher ...
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bioRxiv - Neuroscience 2024Quote: Hippocampi were dissected from neonatal P0 homozygous KIBRA KO or PICK1 KO mice and incubated with 670 μg/mL Papain (Worthington #LS003119) and 100 μg/mL DNase (Sigma-Aldrich #DN-25 ...
Product cited:
Supplied as a lyophilized powder prepared from a 2X crystalline suspension, Code: PAP. To...Cat# LS003119, 100 mg, $100.00 Ask -
bioRxiv - Immunology 2024Quote: ... mice were perfused with 1X PBS and the CNS was isolated into 10 mL of enzyme digestion solution consisting of 75 µL Papain suspension (Worthington, #LS003126) diluted in enzyme stock solution (ESS ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003126, 100 mg, $88.00 Ask -
bioRxiv - Pharmacology and Toxicology 2024Quote: Primary calvarial osteoblasts were isolated from calvaria of 5-day-old neonates using collagenase type II (50 mg/ml, Worthington, LS004176)/dispase II (100 mg/ml ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Immunology 2019Quote: ... Specimens were finely minced between two scalpel blades and incubated for 15 minutes at 37°C with 600 U/mL collagenase IV (Worthington, Lakewood, NJ) and 20 ug/mL DNAse 1 (Roche ...
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Abl kinase deficiency promotes AKT pathway activation and prostate cancer progression and metastasisbioRxiv - Cancer Biology 2020Quote: ... GS689.Li cells from primary tumors were recovered by mincing with a sterile razor blade and digesting with 200 U/ml collagenase II (Worthington Biochemical Corporation) for 15 min at 37°C ...
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bioRxiv - Neuroscience 2020Quote: Brain samples were minced with scissors and then incubated at 37 °C on a roller for 30 min in digest buffer containing 1.25 mg/mL Collagenase Type 4 (Worthington, Lakewood, NJ, USA) and 100 U/mL DNAseI (Sigma ...
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bioRxiv - Bioengineering 2021Quote: The elastin degraded model was produced by incubating native porcine carotid arteries in 10 U/ml purified elastase (ESFF, Worthington Biochemical Corporation) with 0.35 mg/ml trypsin inhibitor (10109886001 ...
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bioRxiv - Cell Biology 2022Quote: ... ovarian cortex samples were digested in DMEM containing 25 mM HEPES and 2 mg/mL collagenase type III (Worthington Biochemical Corporation, LS004183) for 2 hours at 37°C with occasional swirling ...
Product cited:
Lower in secondary proteolytic contaminant activities but with typical collagenase activity. ...Cat# LS004183, 5 gm, $900.00 Ask -
bioRxiv - Physiology 2019Quote: ... An excised muscle was pinned to the bottom of a 3.5 cm Petri dish lined with Silgard and was enzymatically digested using 4mg/ml type-2 collagenase (Worthington Biochemical Corporation, USA) dissolved in HEPES-buffered Tyrode ...
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bioRxiv - Neuroscience 2020Quote: ... Acute bilateral fastigial nuclei were quickly excised with a sharp knife from 2 or 3 cerebellar slices and were then enzymatically digested with cysteine (2 mM)-supplemented papain (40 U/ml, Worthington, Lakewood, NJ) and chondroitinase-ABC (1 U/ml ...
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bioRxiv - Cell Biology 2021Quote: ... BM was flushed and bones were cut into small pieces that were digested in serum free αMEM containing 2mg/ml Collagenase type II (Worthington, Lakewood, NJ) for 2 hours at 37°C in a shaking water bath ...
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bioRxiv - Cell Biology 2021Quote: ... Hemispheres were approximately resected to remove the basal ganglia (Chen et al., 2013) and the cortical regions were placed in 200 arbitrary units/mL papain (Worthington, Cat. #3126) for 5-7 minutes and neutralized with trypsin neutralization solution (Sigma ...
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bioRxiv - Bioengineering 2021Quote: The collagen degraded model was produced by incubating native porcine carotid arteries in 1000 U/ml purified collagenase (CLSPA, Worthington Biochemical Corporation) in magnesium chloride and calcium chloride supplemented PBS (D8662 ...
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bioRxiv - Genomics 2022Quote: ... Bulk mammary tissues were mechanically processed into a slurry and digested overnight with collagenase type 3 (200U/mL, Worthington Biochem CLS-3) and hyaluronidase (100U/mL ...
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bioRxiv - Microbiology 2022Quote: ... Bacterial numbers were enumerated by plating serial dilutions on TSA plates supplemented with 100 µl of catalase (38,000 U/ml; Worthington Biochemical Corporation, NJ) and the desired antibiotic (250 µg/ml kanamycin ...
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bioRxiv - Microbiology 2022Quote: ... The barcoded Spn transformants were selected on TS plates supplemented with 100 µl of catalase (38,000 U/ml; Worthington Biochemical Corporation, NJ) and 200 µg/ml spectinomycin ...
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bioRxiv - Microbiology 2022Quote: ... Cultures were incubated statically at 37°C with 5% CO2 followed by plating on TSA plates supplemented with 100 µl of catalase (38,000 U/ml; Worthington Biochemical Corporation, NJ) and the desired antibiotic (250 µg/ml kanamycin or 200 µg/ml streptomycin) ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatant was then removed and the cells were resuspended in 0.9 ml of the isolation solution containing 100 µl of an ovomucoid inhibitor solution (both Worthington, Lakewood, NJ, USA) and 50 µl of DNase ...
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bioRxiv - Neuroscience 2023Quote: ... the medial PFC of ECFP-expressing P1 mice was dissected and dissociated with papain (20-25 U/mL; Worthington Biochemical, Lakewood, NJ) at 37°C for 30 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... which were immediately chopped and digested at 37°C with rotation in a solution containing 2mg/ml papain (Worthington Biochemical Corporation, LS003126) and 50 μg/ml DNase I in PBS ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003126, 100 mg, $88.00 Ask -
bioRxiv - Developmental Biology 2023Quote: 30 hindbrains of st.18 chick embryos were harvested and disassociated into single cells using collagenase type 4 (200 Units\ml, Worthington 47B9407, USA). Cells were then centrifuged at 600 g for 10 min ...
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bioRxiv - Developmental Biology 2023Quote: ... 60 hindbrains of st.18 chick embryos were harvested and disassociated into single cells using collagenase type 4 (200 Units\ml, Worthington 47B9407, USA). Cells were then centrifuged at 600 g for 10 min ...
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bioRxiv - Neuroscience 2023Quote: ... 4–5 randomly selected organoids were pooled to obtain single cell suspension from hDiO and then incubated in 30 U/mL papain enzyme solution (Worthington Biochemical, LS003126) and 0.4% DNase (12,500 U/mL ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003126, 100 mg, $88.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... Retinas were detached from the retinal pigmented epithelium and incubated for 15 min at 37°C in a solution containing papain (10 U/ml, Worthington; Lakewood, NJ) and L-Cysteine hydrochloride (∼1 mg/mL ...
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bioRxiv - Neuroscience 2023Quote: ... The digestion solution is then aspirated off and the tissue is resuspended in a 2 mg/ml trypsin inhibitor ovomucoid solution (Lo Ovo) (Worthington, Cat# LS003083). Cells were then spun in a centrifuge at 300g for 11 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Cell pellet was collected by centrifuged at 300 g for 5 min and then resuspended in 3 mL Ovomucoid protease inhibitor with FBS (Inhibitor-BSA Vial [LK003182], Worthington, Lakewood, NJ). To remove the debris ...
Product cited:
Ovomucoid protease inhibitor and bovine serum albumin which is 0.22 micron filtered and...Cat# LK003182, 1 vi, $86.00 Ask -
bioRxiv - Physiology 2024Quote: ... CD1 wild type) were harvested and enzymatically digested for 45 min at 37 °C with collagenase type II (520 U/ml, Worthington, NJ US) (20) ...
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bioRxiv - Cell Biology 2024Quote: ... the medullae were rinsed three times in 100 μL drops of papain enzyme solution (450 units/ml Papain; Worthington Biochemical, Cat# LS003126), 250 μg/ml bovine serum albumin (BSA ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003126, 100 mg, $88.00 Ask -
bioRxiv - Neuroscience 2024Quote: ... 4–5 randomly selected organoids were pooled to obtain single cell suspension and then incubated in 30 U/mL papain enzyme solution (Worthington Biochemical, LS003126) and 0.4% DNase (12,500 U/mL ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003126, 100 mg, $88.00 Ask -
bioRxiv - Cancer Biology 2022Quote: ... A ratio of 1:10 wt:wt Trypsin TPCK Treated (Worthington-Biochem) was used to digest the samples for 2h at 47°C ...