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Citations for Worthington Biochemical :
51 - 100 of 130 citations for Recombinant Human 5' Nucleotidase Ecto CD73 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... 5 µg/mL of Oxyrase (Oxyrase Inc.) and 150 U/mL Catalase (Worthington Bio Crop), or on 5% Sheep’s blood agar plates at 370C in a 5% CO2 atmosphere ...
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bioRxiv - Neuroscience 2023Quote: ... and the pellet was resuspended in 5 mL dissociation media containing 165U of Papain (Worthington) and DNAse type I (40 µg/mL ...
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bioRxiv - Immunology 2024Quote: ... before enzymatic digestion in 0.5mL DMEM containing 5 mg/mL type II collagenase (Worthington, # LS004202) and 0.2 mg/mL DNase I (Roche ...
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bioRxiv - Developmental Biology 2019Quote: ... washed in PBS for 5 minutes and digested with 3 mg/ml collagenase type II (Worthington) in HBSS on a rotator at 37°C until no macroscopic tissue was detected ...
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bioRxiv - Cell Biology 2021Quote: ... Minced tissue underwent 3-5 serial digestions in collagenase type 2 (Worthington Biochemical Corp, Lakewood, NJ) in sterile PBS with 20 mM glucose ...
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bioRxiv - Genomics 2020Quote: ... Processing buffer (5% fetal bovine serum in PBS) and DNAse I (100 µg/ml, Worthington LS006344) were then added and the samples rocked at 37°C for 5 minutes ...
Product cited:
Molecular Biology Grade. Chromatographically purified to remove RNase and protease. Supplied...Cat# LS006344, 500 un, $90.00 Ask -
bioRxiv - Neuroscience 2021Quote: ... cut in small pieces and incubated in PBS containing 5 mg/ml of papain (Worthington, LS003124), 0.24 mg/ml of L-cysteine ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003124, 25 mg, $40.00 Ask -
bioRxiv - Genomics 2023Quote: Processing buffer (5% fetal bovine serum in PBS) and DNase I (100 μg/mL, Worthington LS006344) were then added ...
Product cited:
Molecular Biology Grade. Chromatographically purified to remove RNase and protease. Supplied...Cat# LS006344, 500 un, $90.00 Ask -
bioRxiv - Immunology 2024Quote: ... Tissue was digested in perfusion buffer plus 5 mg/ml Collagenase II (Worthington Biochemical Corp; LS004176) for up to 2 hours at 32 °C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Human iPSC-CMs used for patch clamp measurements were dissociated from EHT by collagenase II (200 U/mL; Worthington Biochemical, Lakewood, NJ, USA) for 5 h ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated for 5 minutes at RT in fetal calf serum complemented with DNase I (Worthington), followed by mechanical dissociation ...
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bioRxiv - Immunology 2021Quote: Perfused lungs were finely minced and digested in 5 mL of a 1.78mg/mL Collagenase IV (Worthington) solution in PBS (with Ca2+ ...
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bioRxiv - Cancer Biology 2020Quote: ... tissue was enzymatically digested with 5 mL of papain digestion solution (0.94 mg/mL papain (Worthington #LS003119), 0.48 mM EDTA ...
Product cited:
Supplied as a lyophilized powder prepared from a 2X crystalline suspension, Code: PAP. To...Cat# LS003119, 100 mg, $100.00 Ask -
Interactions with stromal cells promote a more oxidized cancer cell redox state in pancreatic tumorsbioRxiv - Cancer Biology 2020Quote: ... and digested in 5 mL of PBS containing cold 1 mg/mL collagenase I (Worthington Biochemical LS004194), 3mg/mL dispase II (Roche 04942078001 ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004194, 100 mg, $42.00 Ask -
bioRxiv - Immunology 2020Quote: ... Mice were immunized as indicated in each Figure with 5 μg low-endotoxin OVA (Worthington Biochemical Corporation) together with 50 μg papain (P4762 ...
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bioRxiv - Immunology 2023Quote: ... Mice were immunized as indicated in each Figure with 5 µg low-endotoxin OVA (Worthington Biochemical Corporation) mixed with either 50 µg papain (P4762 ...
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bioRxiv - Neuroscience 2024Quote: ... The DRG pieces were incubated in 5 ml HBSS containing 2 mg/mL Stemzyme (LS004106, Worthington Biochemical) and DNAse I (LS001239 ...
Product cited:
A specialized combination of Animal Free Clostridium histolyticum collagenase (Code: CLSAFB) and...Cat# LS004106, 50 mg, $110.00 Ask -
bioRxiv - Cancer Biology 2023Quote: ... minced and digested in 5 mL of Advanced DMEM/F12 containing 10 mg Collagenase Type 3 (Worthington) and 500 U Hyaluronidase (Sigma ...
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bioRxiv - Physiology 2024Quote: ... bubbled with 95% O2/5% CO2) containing collagenase type I (1.5 mg/ml, Worthington, Lakewood, NJ, USA) and 1% bovine serum albumin ...
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bioRxiv - Cancer Biology 2021Quote: ... Right lung lobes were minced and digested with 5 mL digestion buffer: Collagenase 4 (200U/mL, Worthington Biochemicals), DNase I (100 ug/mL ...
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bioRxiv - Immunology 2024Quote: ... Remaining intestinal pieces were then incubated in HBSS containing 5% FBS and 150U/ml collagenase type 2 (Worthington) at 37°C with stirring for 45min to isolate LP cells ...
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bioRxiv - Cell Biology 2023Quote: ... Half of the samples were treated with a final concentration of 5 μg/mL bacterial collagenase (Worthington Biochemical) for 3 d at 37 °C ...
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bioRxiv - Physiology 2021Quote: ... The proteins were digested for 5 hours with Lys-C (Wako) at room temperature and overnight with trypsin (Worthington). Samples were acidified and diluted with TFA to a final concentration of 1% TFA before being loaded on the Sep-Pak Light C18 cartridges (Waters) ...
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bioRxiv - Neuroscience 2021Quote: ... pH adjusted to 7.6] and mechanically dissociated to single cells after adding ovomucoid (Worthington, 0.7 mg per 5 mice) and DNAse (Worthington ...
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bioRxiv - Cancer Biology 2021Quote: ... and resuspended in Dispase II (5 mg/mL; StemCell 7913) and DNase I (100 μg/mL; Worthington Biochemical LS002006) for 5 minutes ...
Product cited:
Chromatographically purified. A lyophilized powder with glycine as a stabilizer.Cat# LS002006, 20 mg, $96.00 Ask -
bioRxiv - Molecular Biology 2019Quote: ... This was achieved by treating the tissue pieces with 5 ml 1 mg/ml elastase (Worthington Biochemical Corporation LS002292) for 30 minutes at 37°C and then 5 ml collagenase added for a further 1-2 hours ...
Product cited:
Two times crystallized, (Code: ESL), supplied as a dialyzed, lyophilized powder. The enzyme...Cat# LS002292, 100 mg, $156.00 Ask -
bioRxiv - Cell Biology 2020Quote: ... lungs were perfused with 5 ml PBS and then digested with 4 U/ml elastase (Worthington Biochemical Corporation, NJ) and 100 U/ml DNase I (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... cells were centrifuged for 5 minutes and the pellet was resuspended with DNAase/ovomucoid inhibitor according to manufacturer’s protocol (Worthington). Cells were then centrifuged and resuspended on ice in Leibovit’z L-15 medium supplemented with 0.1% of bovine serum albumin ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryonic single cell suspensions from were obtained by performing eight rounds of enzymatic digestion (5 minutes each) with Collagenase type II (0.7mg/ml Worthington) at 37°C under agitation ...
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bioRxiv - Immunology 2023Quote: ... 15 mL of pre-warmed digestion solution (1X HBSS with Ca+ and Mg, 5% FBS, 50 U/ml Dispase II [Millipore-Sigma, cat # 4942078001], 0.5 mg/ml DNAse I [Worthington Biochemical ...
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Molecular Insights into the Recognition of Acetylated Histone Modifications by the BRPF2 BromodomainbioRxiv - Biochemistry 2022Quote: ... The nuclei suspension was preincubated at 37°C for 5 min and followed by micrococcal nuclease (Worthington, cat# LS004798) digestion was carried out by following the manufacturer’s protocol ...
Product cited:
Chromatographically purified to be essentially homogeneous chromatographically and...Cat# LS004798, 45 ku, $211.00 Ask -
bioRxiv - Microbiology 2022Quote: ... lung tissue was minced in 5 ml of 1 x PBS containing 3 mg/ml collagenase type IV (Worthington). Samples were incubated at 37°C for 45 min and washed with 1 x PBS three times ...
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bioRxiv - Physiology 2023Quote: ... The muscle layer was cut into pieces and incubated in 5 ml of digestive solution containing 1.5 mg collagenase (Worthington), 1 mg trypsin (Sigma ...
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bioRxiv - Immunology 2023Quote: ... The remaining tissue was then incubated in 5 mL of digest solution [200 U/mL Collagenase IV (Worthington, LS004189) and 400 U/mL DNase I (Worthington ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004189, 5 gm, $900.00 Ask -
bioRxiv - Immunology 2024Quote: ... cut into small ∼1mm3 pieces and enzymatically digested in DMEM containing 5 mg/mL type II collagenase (Worthington, # LS004202) and 0.2 mg/mL DNase I (Roche ...
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bioRxiv - Neuroscience 2021Quote: ... Prepontine regions from 5-6 dpf fish were dissected into Evans buffer and dissociated in 1 mg/ml neutral protease (Worthington) in Evans buffer for 30 min at room temperature with gentle shaking ...
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bioRxiv - Molecular Biology 2020Quote: ... and 5 mM MgCl2) and LC-MS/MS was performed by on-bead digestion with trypsin (TPCK-treated, Worthington Biochemical).
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Pluripotent Stem Cells Can Be Isolated from Human Peripheral Nerves after in vitro BMP-2 StimulationbioRxiv - Cell Biology 2019Quote: ... the minced nerve pieces were pelleted by centrifugation at 500xg for 5 minutes and followed by digestion in 0.2% (0.27 U/ml) collagenase (Worthington Biochemical Corp) at 37°C for 90 minutes ...
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bioRxiv - Neuroscience 2019Quote: ... The cells were then centrifuged at 300*g for 5 minutes and the pellet resuspended in 1 mL of trypsin inhibitor (Worthington) plus DNAse (Sigma ...
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Harvest and Culture of Porcine Adipose-Derived Mesenchymal Stem Cells for Autologous TransplantationbioRxiv - Cell Biology 2020Quote: ... (Fat Processing Video) Phosphate-buffered saline (PBS; 100 mL) was mixed with 75 mg (±5 mg) of type A collagenase (Worthington Biochemical Corporation ...
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bioRxiv - Immunology 2020Quote: ... Approximately 1cm2 of skin was digested overnight in 5%CO2 at 37°C with 3ml of digestion mix containing 0.8mg/ml Collagenase Type 4 (Worthington; #LS004186) and 0.02mg/ml DNase (Sigma-Aldrich ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004186, 100 mg, $42.00 Ask -
bioRxiv - Immunology 2019Quote: ... Approximately 1cm2 of skin was digested overnight in 5%CO2 at 37°C with 3ml of digestion mix containing 0.8mg/ml Collagenase Type 4 (Worthington; #LS004186) and 0.02mg/ml DNase (Sigma-Aldrich ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004186, 100 mg, $42.00 Ask -
bioRxiv - Cancer Biology 2022Quote: ... Tumor pieces were chopped and incubated 5 min at 37°C in a HBSS-papain based lysis buffer (Worthington PAP) containing DNAse (0.01% ...
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bioRxiv - Developmental Biology 2019Quote: ... Ovaries were dissected into 1x PBS on ice and incubated in 5 mg/ml collagenase IV in PBS (Worthington Biochemicals) at RT for 45 min to dissociate the tissue ...
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bioRxiv - Physiology 2023Quote: ... Homogenate was digested with liver digestion buffer (5 ml HBSS w/o Ca2+/Mg2+, 200U/ml Collagenase IV (Worthington, USA) (37 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... at 4°C followed by eight rounds of enzymatic digestion (5 minutes each) with Collagenase type II (1mg/ml Worthington) at 37°C ...
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bioRxiv - Physiology 2023Quote: ... Hearts were then perfused for 5 - 10 min with 0.125 mM CaCl2 KRH buffer supplemented with 1mg/mL of type II collagenase (Worthington Biochemical). Ventricles were cut into 0.1 mM CaCl2 solution ...
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bioRxiv - Immunology 2023Quote: ... The right lobes of the lungs were collected in 5 mL of digestion buffer (90% DMEM and 10% PBS and calcium with type 2 collagenase (180 U/ml) (Worthington) and DNase (15μg/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... The material was then transferred to a 5 mL microcentrifuge tube containing 2.5 mL Papain supplemented with 125 uL DNase (Worthington LK003150) and incubated for 30-45 minutes in a 37°C incubator ...
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bioRxiv - Neuroscience 2021Quote: ... SNc regions from the slices were dissected out with a scalpel blade and digested with oxygenated high glucose HEPES-buffered saline containing papain (5–8 U/ml, Worthington, USA) for 20–30 min at 37°C ...