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Citations for Worthington Biochemical :
1 - 50 of 121 citations for Recombinant Human 5' Nucleotidase Ecto CD73 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Recombinant HIV (Worthington) and HiScript III (Vazyme ...
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bioRxiv - Cancer Biology 2021Quote: ... 100-200 mg of tumor bearing lung tissue was then thoroughly minced with Noyes scissors, prior to incubation with digestion buffer (HBSS supplemented with 5% HI FBS, 125 U/mL collagenase IV (Worthington) and DNase (Roche ...
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bioRxiv - Immunology 2024Quote: ... Enzymatic digestion was stopped with 1 mL of 10X hi ovomucoid inhibitor solution and 20 µL 0.4% DNase (Worthington, #LS002007) diluted in 10 mL inhibitor stock solution (ISS) ...
Product cited:
Chromatographically purified. A lyophilized powder with glycine as a stabilizer.Cat# LS002007, 100 mg, $350.00 Ask -
bioRxiv - Neuroscience 2020Quote: Primary human cortical tissue was dissociated with Papain (Worthington)-containing DNase ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 mg Collagenase IV (Worthington), 25 U Dispase (Corning) ...
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bioRxiv - Cell Biology 2023Quote: ... 5 U/ml elastase (Worthington), and 60 U/ml DNAse I (Roche) ...
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bioRxiv - Neuroscience 2021Quote: ... and 5 U/mL papain (Worthington) (Note ...
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bioRxiv - Cell Biology 2020Quote: ... containing 5 U of papain/ (Worthington) for 30 min at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... containing 5 U of papain/(Worthington) for 30 min at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... 5 U SUPERase•In™ RNase Inhibitor and 5 U HIV RT (Worthington Biochemical Corporation, cat. HIVRT). For TGIRT-III ...
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bioRxiv - Neuroscience 2023Quote: ... Human samples were dissociated according to a modified papain-based protocol (Worthington), then filtered through a 40 µM filter before counting and dilution for scRNAseq.
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bioRxiv - Neuroscience 2019Quote: ... 5 mg/mL Type II collagenase (Worthington) and 2% DNase ...
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bioRxiv - Cell Biology 2022Quote: ... and 5□mg/mL proteinase K (Worthington), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... and 5 mg/mL proteinase K (Worthington), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... RNase T1 (5 U; Worthington Biochemical LS01435), RNase H (8 U ...
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bioRxiv - Neuroscience 2023Quote: ... with 5 units/ml papain (Worthington; # LK003178) and 1mM EDTA ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003178, 5 vi, $98.00 Ask -
bioRxiv - Neuroscience 2021Quote: ... and DNAse (Worthington, 1,000 units per 5 mice). Cells were centrifuged for 10 min at 4°C without brake in 22% Percoll (Sigma ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 5 mg/ml proteinase K (Worthington), in accordance with the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... and collagenase type 1 5 mg/mL (Worthington, LS004194). The ganglia were incubated with the enzyme for approximately 45 minutes before mechanical dissociation with a fire polished Pasteur pipette ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004194, 100 mg, $42.00 Ask -
bioRxiv - Physiology 2022Quote: Human adipocytes were isolated from adipose tissue samples by collagenase (type 1, Worthington, NJ, USA) as described previously [24] ...
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bioRxiv - Cell Biology 2020Quote: ... washed and incubated with 5 Units/ml porcine elastase (Worthington), 100 Kunitz Units/ml DNase I (Worthington) ...
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bioRxiv - Immunology 2021Quote: ... and 5 mg/mL collagenase IV (Cat. # LS004188, Worthington Biochemical) for 2.5 hrs at 37C ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
bioRxiv - Immunology 2023Quote: ... 5 mM EDTA and 0.8 mg/mL collagenase II (Worthington). Following enzymatic digestion tissue was passed through a 70 μm filter and washed with HBSS containing 3% FBS and 5 mM EDTA ...
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bioRxiv - Immunology 2020Quote: ... and digested with collagenase/DNAse I (5 mg/ml, Worthington biochemical) for 30 min at 37° C ...
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bioRxiv - Cell Biology 2020Quote: ... valve leaflets were subjected to 5 mins collagenase (Worthington Biochemical# LS004176) digestion at 37°C and the endothelial layer was gently sheared by sterile swab onto the leaflet ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Neuroscience 2022Quote: ... which contained 5 mL of 0.3 mg/mL papain (Worthington, LS003126) diluted in HBSS ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003126, 100 mg, $88.00 Ask -
bioRxiv - Immunology 2019Quote: Human Omentum was weighed and 0.035 – 1.8g of tissue was digested using 2mg/ml Collagenase I (Worthington) in PBS (Invitrogen/sigma ...
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bioRxiv - Neuroscience 2023Quote: ... washed 5 minutes with Neurobasal media containing Ovomucoid (15 mg/mL, Worthington), and manually triturated in OptiMeM supplemented with D-Glucose (20mM) ...
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bioRxiv - Microbiology 2023Quote: ... 5% NaHCO3) with 1 μg/mL TPCK-treated trypsin (Worthington Biochem. Corporation), then cells were incubated at 37°C ...
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bioRxiv - Biophysics 2024Quote: ... and collagenase Type I (5 mg/mL, Cat# LS004194, Worthington, Lakewood, NJ) and incubated for 45 min at 37°C under gentile agitation ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004194, 100 mg, $42.00 Ask -
bioRxiv - Neuroscience 2021Quote: Differentiated human neural cultures (3 months old) were dissociated into single-cells suspension using Papain Dissociation System (Worthington #LK003150), passed through 70µm and 40µm cell strainers (Falcon #07-201-431 and #07-201-430) ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Paleontology 2019Quote: ... Hyaline articular cartilage was dissected and human chondrocytes isolated using 3 mg/mL Collagenase Type II (Worthington Cat # LS004177) in DMEM medium (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... Tissue was digested by activated papain (Worthington, resuspended in 5 ml HA-Ca) with 100 μl DNase I (2 mg/ml ...
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bioRxiv - Cell Biology 2020Quote: ... 12.5 μM CaCl2 and 5 mg/ml Collagenase II (Worthington Biochemical Corp; LS004176) for 1 hour at 32 °C ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Biophysics 2022Quote: ... supplemented with DNase (5 μg/ml final concentration, Worthington Biochemical Corp., Lakewood, NJ), lysozyme (10 μg/ml final concentration ...
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bioRxiv - Immunology 2023Quote: ... and digested with collagenase II (5 mg/ml in HBSS) (Worthington Biochemical, #LS004176) for 1 hour at 37 □ on shaker at speed of 200 rpm ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Cancer Biology 2024Quote: ... then digested in 5 ml of 1mg/ml Collagenase type V (Worthington LS005282) solution dissolved in HBSS for 20 minutes at 37 °C ...
Product cited:
Prepared to contain higher collagenase and caseinase activities. A dialyzed, lyophilized powder.Cat# LS005282, 1 gm, $246.00 Ask -
bioRxiv - Neuroscience 2021Quote: ... we removed the sheath covering the brain using collagenase (5% in extracellular saline, Worthington Biochemical Corporation Collagenase Type 4 ...
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bioRxiv - Neuroscience 2022Quote: ... that contained 9.5%v/v Ovomucoid Inhibitor and 5%v/v DNase (Worthington Biochemicals). This resuspended solution was incubated at 37°C for 10 minutes and then re-spun at 1500rpm for 5 minutes ...
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bioRxiv - Genomics 2023Quote: ... Processing buffer (5% FBS in 1XPBS) and DNase I (100 μg/mL, Worthington LS006344) were then added ...
Product cited:
Molecular Biology Grade. Chromatographically purified to remove RNase and protease. Supplied...Cat# LS006344, 500 un, $90.00 Ask -
bioRxiv - Neuroscience 2024Quote: ... 5 mM MgCl2 and 10 U/mL DNaseI (Worthington; Code: DPRF; Cat. No. LS006333) were then added to the solution ...
Product cited:
Molecular Biology Grade. Chromatographically purified to remove RNase and protease. ...Cat# LS006333, 10 ku, $176.00 Ask -
bioRxiv - Genomics 2019Quote: ... Dissociated cells were layered on the top of 5 ml of Ovomucoid inhibitor solution (Worthington) and harvested by centrifugation (140 x g for 6 min) ...
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DDK regulates replication initiation by controlling the multiplicity of Cdc45-GINS binding to Mcm2-7bioRxiv - Cell Biology 2020Quote: ... Proteins were released from the DNA by incubation with 5 U of DNA I (Worthington) in 10 mL of 25 mM HEPES-KOH (pH 7.6) ...
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bioRxiv - Microbiology 2022Quote: Lungs were collected and digested at 37 with 5 mg/mL of collagenase I (Worthington) and 1 mg/mL of DNase I (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... The dissociated cells were layered on top of 5 ml of ovomucoid inhibitor solution (Worthington) and harvested by centrifugation (70 x g for 6 min) ...
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bioRxiv - Genetics 2023Quote: ... 5 µg/mL of Oxyrase (Oxyrase Inc.) and 150 U/mL Catalase (Worthington Bio Crop), or on 5% Sheep’s blood agar plates at 370C in a 5% CO2 atmosphere ...
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bioRxiv - Neuroscience 2023Quote: ... and the pellet was resuspended in 5 mL dissociation media containing 165U of Papain (Worthington) and DNAse type I (40 µg/mL ...
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bioRxiv - Developmental Biology 2019Quote: ... washed in PBS for 5 minutes and digested with 3 mg/ml collagenase type II (Worthington) in HBSS on a rotator at 37°C until no macroscopic tissue was detected ...
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bioRxiv - Cell Biology 2021Quote: ... Minced tissue underwent 3-5 serial digestions in collagenase type 2 (Worthington Biochemical Corp, Lakewood, NJ) in sterile PBS with 20 mM glucose ...
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bioRxiv - Genomics 2020Quote: ... Processing buffer (5% fetal bovine serum in PBS) and DNAse I (100 µg/ml, Worthington LS006344) were then added and the samples rocked at 37°C for 5 minutes ...
Product cited:
Molecular Biology Grade. Chromatographically purified to remove RNase and protease. Supplied...Cat# LS006344, 500 un, $90.00 Ask