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Citations for Worthington Biochemical :
651 - 700 of 722 citations for 2 1H Imidazol 1 yl 6 methyl 4 pyrimidinemethanamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Nucleoprotein reassortment enhanced transmissibility of H3 1990.4.a clade influenza A virus in swinebioRxiv - Microbiology 2023Quote: ... the media was supplemented with 1 µg/ml of tosylsulfonyl phenylalanyl chloromethyl ketone (TPCK) treated-trypsin (Worthington Biochemicals, Lakewood, NJ). Wild-type viruses were generated with their respective NP genes (wtNC/19-pdmNP and wtMN/18-trigNP) ...
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bioRxiv - Cancer Biology 2024Quote: Tumor-transplanted organoids were transferred to a 1.7 mL Eppendorf tube containing 1 mL Papain supplemented with 50 uL DNase (Worthington LK003150) and incubated for 30-45 minutes in a 37°C incubator ...
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bioRxiv - Immunology 2024Quote: ... Livers were digested at 37°C for approximately 1 h in DMEM medium supplemented with 500 U/ml Collagenase II (Worthington). Erythrocytes were excluded using red blood cell lysis buffer (BioLegend ...
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bioRxiv - Cancer Biology 2021Quote: Tumor-bearing mice were sacrificed and tumors excised and digested at 37°C for 30 min using a cocktail of 1 mg/ml collagenase type IV (Worthington Biochemicals) and 0.02 mg/ml DNase (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2020Quote: Single myofibers were isolated from extensor digitorum longus (EDL) muscles by 1 hour digestion in 0.2% collagenase type I (Worthington Biochemical Corporation) in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Bioengineering 2020Quote: ... Infection with the H1N1 virus was performed in the presence of 1 µg of TPCK (tolylsulfonyl phenylalanyl chloromethyl ketone)-trypsin/ml (Worthington Biomedical).27 Briefly ...
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bioRxiv - Cell Biology 2021Quote: Epididymal fat pads from mice were minced in Krebs-Ringer phosphate buffer and digested with 1 mg/ml collagenase type I (Worthington Biochemical) at 37°C for 1 h as described in the literature (58) ...
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bioRxiv - Physiology 2021Quote: ... adjusted with NaOH) and then incubated in an enzyme cocktail containing 1 mg/mL papain (Worthington Biochemical Corp., Lakewood, NJ, USA), 1 mg/mL dithiothreitol (DTT ...
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bioRxiv - Cell Biology 2022Quote: ... and the telencephalon was manually cut into pieces, followed by enzymatic digestion (DMEM GlutaMAX 1% P/S, 3% papain (Worthington 3126), 1.5% 10 mg ml−1 DNAse and 2.4% 12 mg ml−1 cystein ...
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bioRxiv - Immunology 2021Quote: Single cell suspensions were generated from Achilles tendons of MaFIA mice by enzymatic digestion with a solution containing 1 mg/mL collagenase I (Cat. # LS004196, Worthington Biochemical) and 5 mg/mL collagenase IV (Cat ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004196, 1 gm, $215.00 Ask -
bioRxiv - Immunology 2020Quote: ... inflated with a 1.5 ml mixture of 1 ml low melting agarose (3% w/v) and 500 μl 4U/ml dispase (Worthington Biochem, LS02104), and incubated for 1 h at room temperature (RT) ...
Product cited:
Animal Free, Chromatographically purified. A lyophilized powder.Cat# LS02104, 50 mg, $352.00 Ask -
bioRxiv - Cell Biology 2021Quote: ... Cortices were digested at room temperature for 1 hour using papain solution composed of 20 units/ml papain (Worthington Biochem, LS003126), 1.5 mM CaCl2 (Sigma Aldrich ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003126, 100 mg, $88.00 Ask -
bioRxiv - Developmental Biology 2020Quote: Two organoids per experimental condition were cut into 1 mm2 pieces and enzymatically digested with pa apain digestion kit (Worthington, LK003163) with the addition of 0.5 mg/ml DNase for 1 hr at 37°C ...
Product cited:
Complete kit as described for product Code: PDS, but without the Earle's Balanced Salt Solution (EBSS).Cat# LK003163, 3 bx, $715.00 Ask -
bioRxiv - Immunology 2020Quote: ... TIL were harvested by dissection of tumor tissue into small fragments in a 50 ml conical tube followed by digestion at room temperature in a bacterial shaker at 180 rpm for 30 minutes in 1 mg/ml collagenase type IV (Worthington Biochemicals), 100 ug/ml hyaluronidase (Sigma-Aldrich ...
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bioRxiv - Biophysics 2022Quote: ... CaCl2 was added to a final concentration of 40 mM (21 µL of 1 M) followed by the addition of 75 U (3 µL of 25 U/µL) of micrococcal nuclease (MNase, Worthington Biochemical) and incubated at 37◦C for 5 min ...
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bioRxiv - Immunology 2024Quote: ... The digested samples were washed with PBS and were further digested with 0.05% trypsin-EDTA for 5 minutes and 1 unit/ml neutral protease (dispase) (Worthington Biochemical, LS02109) plus 100 ug/ml Dnase (Roche ...
Product cited:
Animal Free, Partially purified. A lyophilized powder.Cat# LS02109, 1 gm, $171.00 Ask -
bioRxiv - Immunology 2024Quote: ... these tissues were finely minced and then digested at 37℃ in digestion buffer: RPMI 1640 (UCSF Media Production) with 1 mg/mL collagenase IV (Worthington Biochemical) and 0.1 mg/mL DNase I (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2024Quote: ... Biopsies were mechanically minced with sterile scissors into fragments of less than 1 mm3 and enzymatically digested with 20 mg/mL papain (Worthington #20132), 0.7mg/mL hyaluronidase (Sigma #V900833 ...
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bioRxiv - Genomics 2023Quote: ... Each sample was centrifuged at 1200 rpm x5 mins at 4°C and resuspended in 10 mL warmed digestive media (HBSS + 1% HEPES) with 1,000 U/mL collagenase type IV (Worthington, Cat: LS004188), and rotated for 30 min at 37 °C ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... Cortices were isolated from P0 CD-1 mouse brain tissues and digested with 10 U/mL papain (Worthington Biochemical Corp., LK003176). Mouse cortical neurons were plated on following poly lysine-coated glass bottom dishes (500,000 cells) ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003176, 1 vi, $29.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... Hippocampi were isolated from P0 CD-1 mouse brain tissues and digested with 10 U/mL papain (Worthington Biochemical Corp., LK003176). Mouse hippocampal neurons were plated on following poly lysine-coated dishes for each experiment - glass bottom dishes (500,000 cells ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003176, 1 vi, $29.00 Ask -
bioRxiv - Cell Biology 2023Quote: ... the tissues were further transferred into 20 mL of pre-warmed digestion buffer (RPMI medium containing 10% FBS, 1% P/S, 15 mM HEPES, 25 U/mL of collagenase IV (Worthington LS004186), 100 U/ml of DNase I (Sigma D4513) ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004186, 100 mg, $42.00 Ask -
bioRxiv - Cancer Biology 2023Quote: Tumor-bearing mice were sacrificed and tumors excised and digested at 37°C for 30 min using a cocktail of 1 mg/ml collagenase type IV (Worthington Biochemicals) and 0.02 mg/ml DNase (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... hACs were isolated using 10 ml of a 0.15% (w/v) type II collagenase (300 U mg−1; Worthington Biochemical Corporation) solution per g of tissue (37 °C ...
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bioRxiv - Cancer Biology 2023Quote: The largest tumors from female C3-TAg and PyMT mice were minced and dissociated for up to 120 min at 37°C in a mixture of 1 mg/ml Collagenase (Worthington, LS004188), 20 U/ml DNase (Invitrogen ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
bioRxiv - Developmental Biology 2023Quote: Two organoids per experimental condition were washed with Ca2+/Mg2+-free DPBS and cut into 1 mm2 pieces and enzymatically digested with papain digestion kit (Worthington, LK003163) with the addition of DNase for 1 hr at 37°C ...
Product cited:
Complete kit as described for product Code: PDS, but without the Earle's Balanced Salt Solution (EBSS).Cat# LK003163, 3 bx, $715.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... Homogenate was digested for 15 minutes in enzymatic solution (0.2 µm filter sterilised 4.5 U/mL papain [Worthington Biochemicals], 1× DNAse [Worthington Biochemicals], in PBS) at 37 °C ...
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bioRxiv - Bioengineering 2023Quote: ... constructs were extracted from devices and incubated on an orbital shaker for 1 hour in a solution of 0.15% type II collagenase (Worthington Biochemical Corporation) in DMEM ...
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bioRxiv - Cell Biology 2024Quote: ... hindlimb muscles from mice and hamstring muscles from humans were digested with collagenase II (LS004177, Worthington, 1000 units per 1 ml) for 90 min at 37 °C ...
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bioRxiv - Neuroscience 2024Quote: ... Terminal differentiation of oligodendrocytes was achieved in oligodendrocyte differentiation media by mitogen withdrawal for 7 days (except for T3 and IGF-1) following single-cell dissociation using Papain (20 units/ml) dissociation kit (Worthington Biochemical) and plating on Matrigel (SLS ...
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bioRxiv - Microbiology 2024Quote: ... 1% MEM vitamin solution and freshly added 1.0 μg/ml tosylsulfonyl phenylalanyl chloromethyl ketone (TPCK)-trypsin (Worthington Biochemical Corporation, Lakewood, NJ) incubated at 37 °C ...
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bioRxiv - Developmental Biology 2024Quote: ... minced vessels were incubated for 60 minutes at 37°C with agitation in an enzymatic cocktail consisting of 1 mg/ml collagenase I (Worthington, LS004196), 1 mg/ml Dispase II (Gibco ...
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bioRxiv - Bioengineering 2022Quote: ... and then inflated via the trachea with 2-3 mL of pre-warmed (37°C) enzyme solution (1 mg/mL collagenase/dispase [Roche], 3 U/mL elastase [Worthington], 20 U/mL DNase [Worthington] in DMEM). The heart and large airways were dissected away and the remaining lung tissues and enzyme solution were collected and transferred to a shaking 37°C water bath at 75 rpm for 20 min ...
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bioRxiv - Neuroscience 2020Quote: ... The collected nerve segments were cut into small pieces with microscissors and incubated in 1 ml collagenase (4mg/ml Worthington Biochemical, LS004176) and dispase (2mg/ml ...
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bioRxiv - Genomics 2021Quote: ... Lung tumor sample were cut into small pieces with a razor blade and enzymatically digested (100 U/mL Collagenase IV, Sigma-Aldrich, C5138-1G; 50 µg/mL DNase 1, Worthington, LS002138) for 35 minutes being rotated at 37°C in HEPES buffered RPMI 1640 containing 0.5% FBS ...
Product cited:
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bioRxiv - Neuroscience 2020Quote: ... The collected nerve segments were cut into small pieces with microscissers and incubated in 1 ml PBS supplemented with collagenase (4mg/ml Worthington Biochemical, LS004176), dispase (2mg/ml ...
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bioRxiv - Immunology 2020Quote: To prepare single-cell suspensions lungs were finely chopped with scissors in a 48 well plate and treated with 3 mg ml-1 collagenase III (Worthington, Lakewood, NJ), 5 μg ml-1 DNAse ...
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bioRxiv - Immunology 2021Quote: ... tissue was finely minced and placed into digestion media (RPMI basal media, 300 U/mL collagenase I [Worthington Biochemical Corporation], 1% BCS), shaken for 30 min at 37°C ...
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bioRxiv - Immunology 2020Quote: Lungs from 8-10 week old mice were dissected and mechanically disrupted using scissors before incubation in RPMI containing 1 μg/ml type II collagenase (Worthington Biochemical Corporation) and 40 U/ml DNase I (Sigma Aldrich ...
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bioRxiv - Immunology 2023Quote: ... The whole lung was minced and enzymatically digested in RPMI 1640 medium containing 1 mg/ml collagenase IV (Worthington Biochemical, Lakewood, NJ) at 37◦C for 30 min ...
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bioRxiv - Neuroscience 2024Quote: ... Dissected frontal cortex (cortex rostral to the optic chiasm) was minced and immersed in an enzyme incubation solution (1 mL) containing 200 U papain (Worthington Biochemical, #LS003126), 0.5 mM EDTA (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... The DRG bulb was then cut into 3mm sections and placed in 5mL of pre-warmed digestion enzyme containing 1 mg/mL of Stemzyme I (Worthington Biochemical, LS004106), 0.1 mg/mL of DNAse I (Worthington Biochemical ...
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bioRxiv - Neuroscience 2024Quote: Neuronal cells were extracted from the brains of 1-day-pold SD pups using a papain dissociation system (Worthington Biochemical Corporation, Worthington, NJ, USA) following the manufacture’s instruction ...
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bioRxiv - Cell Biology 2024Quote: ... Two to three pieces of 0.5g testis tissue was placed in a 50mL Falcon tube with 10mL prewarmed collagenase solution at 32 °C (1 mg/mL collagenase I (Worthington Biochemicals #LS004196), 1 mM EDTA ...
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bioRxiv - Cell Biology 2020Quote: ... and transferred to a 15ml tube with 8ml collagenase solution (∼4,000 units in αMEM with 1% anti-mycotic; Type IV, Worthington Biochemical Corp, Lakewood, NJ). The solution containing the tissue fragments was then continuously agitated for 1 hour at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Homogenised tissue was added to a solution (total volume of 7 ml) composed by of 1 mg/ml collagenase IV (Worthington Biochemical Corp, USA), 0.02 mg/ml DNase 1 (New England Biolabs ...
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bioRxiv - Cell Biology 2021Quote: ... A single cell suspension of vascular stromal cells was isolated from minced iBAT (using scissors) digested for 90 min with collagenase IV (3 mg mL-1, Worthington, Lakewood NJ, USA) at 37 °C under gentle agitation16 ...
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bioRxiv - Immunology 2021Quote: ... Collected lobes were incubated for one hour at 37°C in 10 mL of cRPMI with 1500 units of 260 units/mg type 1 collagenase (Worthington Biochemical, Cat. #LS004194) per lobe and pressed through a 35 µm filter ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004194, 100 mg, $42.00 Ask -
bioRxiv - Microbiology 2021Quote: ... followed by 10X dilution into 50 mM ABC (pH 8.0) and tryptic digestion at 37°C for 20 hr with shaking using 1 μg of trypsin (Worthington Biochemical Corp., Lakewood, NJ) per 50 μg protein ...
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bioRxiv - Cancer Biology 2022Quote: ... The obtained liver tissues were homogenized in 8 mL of RPMI1640 media containing 1.8 mg/ml Collagenase IV (WOR-CLS4-1, Worthington Biochemical Corporation, NJ, USA) and 112.3 µg/ml DNase I (11284932001 ...