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Citations for Worthington Biochemical :
601 - 650 of 722 citations for 2 1H Imidazol 1 yl 6 methyl 4 pyrimidinemethanamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Minced tissue was then transferred to a 1.5 mL microcentrifuge tube with 1 mL of PBS supplemented with 125 U/mL collagenase IV (Worthington) and DNase (Roche ...
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bioRxiv - Cell Biology 2020Quote: Endometrial tissues were minced into 1 mm3 pieces and dissociated in phosphate-buffered saline (PBS) containing collagenase type III (0.3 mg/ml, Worthington Biochemical Corporation ...
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bioRxiv - Biochemistry 2020Quote: ... DRGs were digested in 1 mL of Ca2+/Mg2+-free HBSS containing 20 U of papain (Worthington, Lakewood, NJ), 0.35 mg of L-cysteine and 1 μL of saturated NaHCO3 and incubated at 37°C for 10 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... and digested for 1 hour at 37° C in DMEM supplemented with 25 U/ml papain (Worthington Biomedical Corp.), 0.2 mg/ml cysteine (Sigma) ...
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bioRxiv - Microbiology 2020Quote: ... A saturated overnight LB culture was diluted 1:50 into 3mL of minimal medium with or without 0.2g/L cellulase (Worthington, purified exo- and endo-glucanases ...
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bioRxiv - Neuroscience 2022Quote: Fresh retinae were dissected before being placed in activated papain/DNase (1:20 solution; Worthington Biochemicals, New Jersey, USA) and incubated at 37°C for 45 minutes on a shaker ...
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bioRxiv - Immunology 2021Quote: ... Freshly isolated tumor tissues were minced into approximately 1 mm3 cubic pieces and digested using 0.1% collagenase IV (LS004186, Worthington), 0.002% DNAse I (D8071 ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004186, 100 mg, $42.00 Ask -
bioRxiv - Immunology 2022Quote: Skin tissued were finely minced with scissors and incubated in a digestion cocktail containing collagenase IV (3.2 mg.ml−1, Worthington, LS004186) and DNAse (20 μg ml−1 ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004186, 100 mg, $42.00 Ask -
bioRxiv - Developmental Biology 2022Quote: ... MM from E11.5 kidneys from CD-1 mice were dissociated with Collagenase Type 3 (Worthington Biochemical Corporation; Cat #LS004180) and DNase I (New England Biolabs ...
Product cited:
Lower in secondary proteolytic contaminant activities but with typical collagenase activity. ...Cat# LS004180, 100 mg, $42.00 Ask -
bioRxiv - Developmental Biology 2022Quote: ... and digested with gentle rocking for 1 h at 37°C in 3 mg/mL collagenase II (Worthington, LS004176) diluted in HBSS containing 5% fetal bovine serum (FBS) ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Cancer Biology 2021Quote: Excised tumors were minced with scissors and enzymatically digested in RPMI medium containing 2.1 mg/ml collagenase type 1 (Worthington), 75 µg/ml DNase I (Worthington) ...
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bioRxiv - Cell Biology 2024Quote: Fragments of calcified valve were washed in PBS and treated with collagenase II (1 mg/ml; Worthington Biochemical Corporation) in PBS for 10 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... The minced tumors were digested with 5ml to 10ml of 1 mg/ml Collagenase 3 (Worthington cat. no. LS004183) plus 0.5mg/ml Hyaluronidase (Sigma-Aldrich H3884 ...
Product cited:
Lower in secondary proteolytic contaminant activities but with typical collagenase activity. ...Cat# LS004183, 5 gm, $900.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... The collected tissue was incubated with continuous shaking at 37°C for 45 min in 1 ml of papain solution (20 U/ml) and 0.2 ml DNase (both from Worthington) prepared in isolation buffer ...
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bioRxiv - Microbiology 2022Quote: ... lung tissue was minced in 5 ml of 1 x PBS containing 3 mg/ml collagenase type IV (Worthington). Samples were incubated at 37°C for 45 min and washed with 1 x PBS three times ...
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bioRxiv - Neuroscience 2023Quote: Neurons were isolated from brains of 1-day-old SD pups using papain dissociation system (Worthington Bio-chemical Corporation) following manufacturer’s instruction ...
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bioRxiv - Microbiology 2023Quote: ... 3 mL of overlay (1.2% Avicel in FBS-free DMEM supplemented with 0.1 mg/mL DEAE dextran and 1 μg/mL Worthington’s trypsin) was added to each well and plates were incubated for 3-4 days ...
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bioRxiv - Immunology 2023Quote: ... The decidua was mechanically dissociated and digested in RPMI-1640 supplemented with 40 U ml-1 DNAse I (Worthington) and 300 U ml-1 collagenase I (Worthington ...
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bioRxiv - Pathology 2023Quote: ... Native SMCs for patch-clamp experiments were obtained by initially digesting isolated arteries in 1 mg/mL papain (Worthington Biochemical Corporation ...
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bioRxiv - Bioengineering 2023Quote: ... or in vivo transplantation using an adaptation of a well-established neural cell isolation method (Worthington 9035-81-1).71 As with our undifferentiated hiPSC cultures ...
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bioRxiv - Bioengineering 2023Quote: Cells were isolated from semitendinosus muscles of Belgian Blue cattle by collagenase digestion (CLSAFA, Worthington; 1 h; 37 °C), filtration with 100 μm cell strainers ...
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bioRxiv - Neuroscience 2024Quote: Neuronal cells were extracted from the brains of 1-day-pold SD pups using a papain dissociation system (Worthington Biochemical Corporation ...
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bioRxiv - Cell Biology 2024Quote: Kidneys were minced and digested for 20 minutes in 1 mg/mL Collagenase A (Worthington Biochemical, Lakewood, New Jersey). Isolates were pushed through 50 mL conical tube sterile 100 µm cell strainers and washed with cold 35 mL HBSS (Gibco ...
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bioRxiv - Cell Biology 2024Quote: Mouse back skin and wound beds were dissected and digested into single cells using 1:100 collagenase 1A (Worthington) and Liberase TM for macrophage subsets ...
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bioRxiv - Cell Biology 2020Quote: ... tumors were mechanically dissociated and subjected to enzymatic digestion for 20 min with 200 U ml−1 collagenase IV (Worthington), 5 mM CaCl2 ...
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bioRxiv - Cell Biology 2022Quote: ... Frontal and parietal bones were minced and subjected to six sequential enzymatic digestions with a mixture containing 1 mg/mL collagenase type I (Worthington Biochemical Corporation ...
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bioRxiv - Neuroscience 2021Quote: ... cerebella from litters of P6 mice were pooled and digested at 37 °C for 20 minutes in 10U ml−1 papain (Worthington), and 250U ml−1 DNase in EBSS using the Papain Dissociation Kit (Worthington ...
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bioRxiv - Developmental Biology 2020Quote: ... followed by quenching of the enzyme using 1 mg/ml ovomucoid/bovine serum albumin solution with 0.1 mg/ml DNase I (Worthington LK003150). Cells were passed through a 70 μm strainer (PluriSelect 43-10070-70 ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Neuroscience 2021Quote: ... permeabilized cells were incubated with DNAse I recombinase (3-3000 U/ml in 50 mM Tris-HCL, pH 7.5, 1/ mg/ml BSA: Worthington, LK003170) prior to incubation with TUNEL mixture.
Product cited:
A component of the Papain Dissociation System. This material is 0.22 micron membrane filtered...Cat# LK003170, 1 vi, $28.00 Ask -
bioRxiv - Immunology 2020Quote: ... Pieces were further digested for 30 min and then twice for 20 min with a mixture of Collagenase IV (157 Wuensch units ml−1, Worthington), DNAse I (0.2 mg ml−1 dissolved in PBS ...
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bioRxiv - Bioengineering 2020Quote: ... The dissected strips of HTM tissue were digested for 20 min with 1 mg/ml collagenase (Worthington, Lakewood, NJ, USA) and 15 μl/ml human albumin (Sigma-Aldrich ...
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bioRxiv - Physiology 2021Quote: ... The arteries were then washed three times with Mg2+-PSS and incubated in 1 mg/mL collagenase type II (Worthington) in Mg2+-PSS for 14 minutes ...
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bioRxiv - Microbiology 2020Quote: ... All viruses were titrated using 1:10 serial dilution in triplicate in LLCMK2 cells in the presence of 2μg/mL TPCK trypsin (Worthington Biomedical) for 72 hours and TCID50/mL was determined by the Reed-Meunch method based on hemagglutination of 0.5% washed chicken red blood cells (Lampire ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell lines were established from autochthonous lung tumors by microdissection and mechanical mincing in digestion buffer (HBSS with 1 M HEPES, 125 Units/mL Collagenase Type IV (Worthington) and 20 μg/mL DNAse (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2022Quote: ... The distal lung tissue was separated from the mainstem bronchi and transferred to a Petri dish containing DMEM with 1% HEPES and 100 U/mL DNase I (Worthington). The tissue was then dissociated with a blunt bone marrow aspiration needle ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were removed and the lateral SVZs were dissected in cold DMEM-F12-glutamax 1/50 Glc 45% and subsequently incubated in digestion medium (papain [Worthington] ...
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bioRxiv - Microbiology 2021Quote: ... 0.1% Bovine Serum Albumin (BSA) (MP Biomedical) and 1 µg/ml of TPCK (tolylsulfonyl phenylalanyl chloromethyl ketone)-trypsin (Worthington Biomedical) (Weingartl et al. ...
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bioRxiv - Immunology 2022Quote: ... which consisted of HBSS containing Ca2+ and Mg2+ supplemented with 3.4 mg/ml collagenase type 1 (Worthington Biochemical Corporation LS004196) and 100 U/ml hyaluronidase (Sigma-Aldrich H3631) ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004196, 1 gm, $215.00 Ask -
bioRxiv - Physiology 2022Quote: ... before perfusion was switched to EGTA-free isolation buffer supplemented with 1 mg/mL collagenase (type II; Worthington Biochemical, USA), 0.06 mg/mL protease XIV ...
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bioRxiv - Immunology 2020Quote: Explanted hearts were cut into pieces and digested for 30 min at 37C with 1 mg/ml of collagenase IV (Worthington) and 0.01% DNase I (Roche) ...
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bioRxiv - Physiology 2020Quote: ... muscles were surgically removed and enzymatically dissociated at 37 °C under mild agitation for ~1 h using 1,000 U/mL of collagenase type IV (Worthington Biochemical). Mechanical dissociation was completed using mild trituration in buffer with no collagenase ...
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bioRxiv - Genomics 2020Quote: ... After removal of the epidermal sheets, the dermis was finely minced and enzymatically digested (RPMI basal medium, 1000U/mL collagenase type 1 (Worthington), 40µg/mL DNase I (Roche) ...
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bioRxiv - Immunology 2022Quote: ... the digested skin was minced and further processed through a 37ºC incubation with 0.2% Collagenase Type 1 (Worthington Biochemical Corporation) in DMEM-F12+ 10% FBS for 1 1/2 hour ...
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bioRxiv - Immunology 2022Quote: ... the digested skin was minced and further processed through a 37ºC incubation with 0.2% Collagenase Type 1 (Worthington Biochemical Corporation) in DMEM-F12+ 10% FBS for 1 1/2 hour ...
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bioRxiv - Neuroscience 2022Quote: ... Retinas were washed 3 times for 3 min in L-15 solution and then placed in L-15 solution containing 7 U ml-1 papain (cat. no. 3126, Worthington Biochemical Corporation ...
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bioRxiv - Cell Biology 2023Quote: ... limb buds from the E12.5 mouse embryo were isolated and digested with 1 mg/ml type II collagenase (Worthington, LS004176) for 1 h at 37 °C ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Immunology 2024Quote: ... Enzymatic digestion was stopped with 1 mL of 10X hi ovomucoid inhibitor solution and 20 µL 0.4% DNase (Worthington, #LS002007) diluted in 10 mL inhibitor stock solution (ISS) ...
Product cited:
Chromatographically purified. A lyophilized powder with glycine as a stabilizer.Cat# LS002007, 100 mg, $350.00 Ask -
bioRxiv - Cell Biology 2023Quote: ... The minced muscles were centrifuged at 15,000 x g for 1 minute and transferred to 10 ml of digestion buffer (DMEM containing 2.5% HEPES, 1% penicillin/streptomycin, and 400 U/ml collagenase II (Worthington Biochemical)) and placed on an orbital shaker at 100 rpm for 1 hour at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Mammary glands were minced into 1 mm3 size using a tissue cutting blade and digested with 0.5 mg/mL Collagenase type III (Worthington, LS004182) and 50 U/mL hyaluronidase (Worthington ...
Product cited:
Lower in secondary proteolytic contaminant activities but with typical collagenase activity. ...Cat# LS004182, 1 gm, $215.00 Ask -
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Digestion was performed by applying 7 mL 1× HBSS containing 600 U/mL Collagenase II (Worthington Biochemical, Lakewood, NJ, USA) and 60 U/mL DNase I (AppliChem ...