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Citations for Qiagen :
301 - 350 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: RNA was extracted from MEF cells using the RNeasy Mini Kit (Qiagen) following manufacturer instructions ...
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bioRxiv - Biochemistry 2024Quote: ... Initial screening was conducted using the sitting-drop vapor diffusion method with the ARI Crystal Gryphon Robot (ARI) and crystallization screening kits (QIAGEN) at 18°C ...
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bioRxiv - Biochemistry 2024Quote: ... and 0.1 mg/ml RNase A (QIAGEN)] ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... the samples were homogenized using a TissueLyser II (Qiagen) with a mixture of 2.8 mm ceramic (VWR) ...
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bioRxiv - Bioengineering 2024Quote: ... and was then subjected to Ni-NTA affinity purification at 4 [(Qiagen, Valencia, CA). The column was adequately washed with 20 column volume of wash buffer containing 50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Bioengineering 2024Quote: ... The output of differentially expressed proteins was further investigated using Ingenuity Pathway Analysis® (IPA; Qiagen®, Redwood City, USA). Data was also independently analysed using PEAKS studio (Bioinformatics Solutions® Inc. ...
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bioRxiv - Bioengineering 2024Quote: DNeasy Blood and Tissue kit (#69504, QIAGEN) was used to extract DNA from samples according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2024Quote: ... total RNA was extracted using RNeasy kits (Qiagen, Valencia, CA) and quantified by NanoDrop (BioLabNet ...
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Phospho-KNL-1 recognition by a TPR domain targets the BUB-1–BUB-3 complex to C. elegans kinetochoresbioRxiv - Cell Biology 2024Quote: ... Beads were washed twice with re-suspension buffer and once with 20 mM imidazole in resuspension buffer before applying to polypropylene columns (Qiagen). Samples were eluted in 1 mL fractions using 400 mM Imidazole in resuspension buffer ...
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bioRxiv - Cell Biology 2024Quote: ... All qPCR assays were performed on Rotor-Gene Q thermocycler (Qiagen, Toronto, ON, Canada). The relative expression of mRNA and miRNA was normalized to ACTB and snRNA U6 ...
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bioRxiv - Cell Biology 2024Quote: ... The genomic DNA fragment was separated by electrophoresis using a 1% agarose gel and purified using QIAquick Gel Extraction Kit (QIAGEN). The genome sequence of drp-1 was determined by Sanger sequencing using primers listed in Supplementary Table S2.
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bioRxiv - Cell Biology 2024Quote: Total RNA of ECs and liver macrophages was isolated with RNeasy plus Mini Kit (Qiagen). Total RNA of the liver tissues was extracted with Trizol (Thermo) ...
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bioRxiv - Cell Biology 2024Quote: ... All DNA fragments used for cloning were purified using a gel purification kit/protocol (Qiagen). All PCR was performed using KOD polymerase (EMD Millipore) ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was purified from mouse tissues using the RNeasy Plus Mini Kit (Qiagen) and then transcribed into cDNA using the iScript™ cDNA Synthesis Kit (Bio-Rad) ...
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bioRxiv - Bioengineering 2024Quote: ... RNA was isolated using the Qiagen RNeasy Kit (Qiagen, Hilden, Germany) following the manufacture’s instruction ...
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bioRxiv - Bioengineering 2024Quote: ... PCR amplification was done with Qiagen’s HotStarTaq Master Mix Kit (Qiagen, Cat. No. 203443), with 20 ng of converted DNA and 0.2 µM for each of primers in 30 µL final volume ...
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bioRxiv - Bioengineering 2024Quote: Total RNA was isolated from untreated or RNP-transfected plerixafor-mobilized HD and SCD HSPCs (n=3 for each group) using the RNeasy Kit (QIAGEN) that includes a DNAse treatment step ...
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bioRxiv - Bioengineering 2024Quote: Genomic DNA was isolated using a DNeasy Blood and Tissue Kit (Qiagen) and PCR amplification was performed with KAPA2G Robust PCR kits (KAPA Biosystems ...
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bioRxiv - Bioengineering 2024Quote: RNA was harvested from cell pellets using the RNeasy Plus Mini Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... the cDNA was synthesized from the extracted total RNA using the miRCURY LNA RT Kit (Qiagen). Briefly ...
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bioRxiv - Biochemistry 2024Quote: ... cells were transfected in 10 cm2 at ∼60% confluency using PolyFect (Qiagen, 301107) and washed with complete culture medium 6 h later ...
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bioRxiv - Biochemistry 2024Quote: Total RNA was isolated from bacterial cells using the RNeasy mini kit (5 mL culture) and RNeasy maxi kit (50 mL culture) (Qiagen). RNA concentrations were measured using a Nanodrop spectrometer (ThermoScientific) ...
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bioRxiv - Biochemistry 2024Quote: ... Tissues were homogenized using Handy Micro Homogenizer (NS310E2, Microtec Co. Ltd., Japan) with 40 µL / mg tissue of RLT buffer (RNeasy Mini Kit, Qiagen, USA), which contained 10 µL / mL of β-mercaptoethanol (β-ME) ...
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bioRxiv - Biochemistry 2024Quote: ... Total RNA was extracted from the supernatant (12,000 x g for 10 min at 4°C) of centrifuged tissue homogenate using an RNeasy Mini Kit (Qiagen, USA). The extracted total RNA was used for single-strand cDNA synthesis ...
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bioRxiv - Bioengineering 2024Quote: ... Total RNA was isolated from harvested cells by using the RNeasy Plus Mini Kit (Qiagen; 74134) following the manufacturer’s recommended protocol ...
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bioRxiv - Bioengineering 2024Quote: Genomic DNA was extracted using the DNeasy Blood & Tissue Kit (Qiagen) according to the manufacture’s instructions ...
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bioRxiv - Bioengineering 2024Quote: Genomic DNA extraction was performed with the DNeasy Blood & Tissue Kit (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: Splenocyte derived mRNA was harvested using a QIAmp RNA mini kit (Qiagen, 52304) and converted to cDNA using RNA to cDNA EcoDry Premix (Takara ...
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bioRxiv - Biochemistry 2024Quote: ... using Effectene transfection reagent (Qiagen, Germantown, MD).
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bioRxiv - Biochemistry 2024Quote: ... Supernatants were filtered through a 0.22 µm syringe filter before application to 5 ml of nickel resin (Ni-NTA Superflow, QIAGEN) equilibrated in loading buffer (25 mM HEPES-KOH pH 7.6 ...
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bioRxiv - Biochemistry 2024Quote: Selected cells were harvested for genomic DNA preparation using DNeasy Blood and Tissue kit (Qiagen, Germantown, MD). 2 µg of genomic DNA was digested with one of few combinations of restriction enzymes (Table S5 ...
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bioRxiv - Biochemistry 2024Quote: Total RNA was isolated from cells grown in six-well plates at 70–80% confluency with an RNA extraction kit (Qiagen #74104). cDNA was synthesized using a sensiFAST cDNA synthesis kit (Meridian Bioscience #BIO-65053) ...
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bioRxiv - Biochemistry 2024Quote: ... The lysate was pelleted by centrifugation at 30,000 x g for 30 min and the supernatant was mixed with 5 mL Ni-NTA resin (Qiagen) pre-equilibrated with lysis buffer in a 50 mL falcon tube ...
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bioRxiv - Biochemistry 2024Quote: ... Ingenuity Pathway Analysis (Qiagen, Germany); R studio (Version1.2.5001) ...
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bioRxiv - Bioengineering 2024Quote: ... Genomic DNA and RNA were isolated from homogenized tissues using the DNEasy Blood and Tissue Kit (Qiagen, LLC) and RNeasy Kit (Qiagen ...
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bioRxiv - Bioengineering 2024Quote: ... The sized samples were purified with MinElute columns (Qiagen, Hilden, Germany) and started in library prep with 10ng each for the ‘long’ samples and 8ng each for the ‘short’ ...
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bioRxiv - Bioengineering 2024Quote: ... The tissue was pulverized (6775 Freezer/Mill SPEX) and the total RNA was extracted with RNeasy minicolumns (RNeasy Fibrous Tissue Kit, Qiagen). RNA concentration was quantified using a Qubit 4 Fluorometer (Thermo Fisher) ...
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bioRxiv - Biochemistry 2024Quote: ... Ingenuity Pathway Analysis (Qiagen, Germany); R studio (Version1.2.5001) ...
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bioRxiv - Biochemistry 2024Quote: ... PCR arrays were performed using RT2 Profiler™ PCR Array Mouse Cytokines & Chemokines (PAMM-150Z, Qiagen). Calculations were performed by a comparative method (2−ΔΔCt ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were detached by trypsin treatment and RNAs were extracted using miRNeasy Mini Kit (Qiagen). RNA concentration was determined by NanoDrop One C (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... Solubilized BasC-GFP was bound to Ni-NTA Agarose (Qiagen) previously equilibrated with DM or DDM buffer (TB with 0.06 % DDM or 0.17 % DM ...
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bioRxiv - Biochemistry 2024Quote: ... The proteins were purified using Ni2+ affinity chromatography (Ni-NTA Superflow, Qiagen). Eluted protein was concentrated and passed over a Superdex 200 Increase 10/300 GL size exclusion column (Cytiva ...
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bioRxiv - Biochemistry 2024Quote: ... protein purification was performed using Co+NTA agarose (Qiagen), followed by a Phenyl Sepharose column ...
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bioRxiv - Bioengineering 2024Quote: ... RNA was extracted from samples using the RNeasy Micro Kit (QIAGEN INC, USA) according to manufacturer instructions with DNase application and eluted in 15 µL RNase free water ...
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bioRxiv - Bioengineering 2024Quote: ... RNA was extracted from samples using the RNeasy Micro Kit (QIAGEN INC, USA) according to manufacturer instructions and eluted in 15 µL RNase free water ...
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bioRxiv - Bioengineering 2024Quote: ... DNA extraction was carried out using a Qiagen DNeasy PowerBiofilm Kit (Qiagen, Hilden, Germany), employing a detergent lysis method in conjunction with bead beating using an MPTM FastPrep-24 homogenizer (MP Biomedicals ...
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bioRxiv - Bioengineering 2024Quote: ... 1 mL of 50% Ni-NTA agarose (Qiagen, Toronto, Ontario, Canada) was equilibrated using 10 bed volumes of wash buffer (same formulation as lysis buffer) ...
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... RNA was extracted using the QIAGEN RNeasy Mini Kit following the manufacturer’s protocol (QIAGEN, # 74106) and quality control was performed using a Bioanalyzer (Agilent).
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bioRxiv - Bioengineering 2024Quote: ... Frozen aortic samples were finely ground into powder using a pre-chilled mortar and pestle to purify RNA using the RNeasy mini kit as per manufacturer instructions (Qiagen; Hilden, Germany). Quantity and quality of RNA was measured using a Nanodrop ND-1000 spec-trophotometer (Thermo Scientific ...
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bioRxiv - Bioengineering 2024Quote: Whole mouse aortas (n=18 per group) cleaned of perivascular tissue were perfused with sterile HBSS and stabilized overnight at 4◦C in RNAProtect reagent (Qiagen; Hilden, Germany) before long-term storage at -80◦C ...