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Citations for Qiagen :
551 - 600 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Lysates were subsequently passed through a QIAshredder column (Qiagen, 79654) to eliminate debris before proceeding with nucleic acid purification ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was isolated with the RNeasy mini kit (Qiagen, Venlo, The Netherlands) and mRNA expression was determined with the HT-12 v4 chip (Illumina ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA extractions from each sample were performed using RNeasy Fibrous Tissue Mini Kit (Qiagen). Extracted total RNA quality and concentration were assessed on a NanoDrop Spectrophomoter (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... cellular RNA was extracted using the RNeasy mini kit (Qiagen, Hilden, Germany) using the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... The inverse PCR products were gel-extracted using the QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany) and sent for sanger sequencing ...
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bioRxiv - Molecular Biology 2024Quote: ... The NS segment was amplified using the Omniscript RT Kit (Qiagen, Germany) and Phusion® High-Fidelity DNA Polymerase (New England Biolabs ...
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bioRxiv - Molecular Biology 2024Quote: ... Ni-NTA magnetic agarose beads (QIAGEN) were added to His-tagged FL-ERRα and His-NanoLuc (= neg ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was loaded on a 1 ml Ni-NTA Superflow cartridge (QIAGEN). The column was washed with 10 column volumes (CVs ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was isolated using RNeasy Micro Kit (Qiagen). RNA from the murine livers was isolated using TRIzol Reagent ...
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bioRxiv - Molecular Biology 2024Quote: ... and RNA extraction was performed using RNeasy Plus Micro Kit (74034 QIAGEN). RNA quality test was performed by Agilent High Sensitivity RNA ScreenTape System (G2964AA) ...
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bioRxiv - Neuroscience 2024Quote: ... Overnight cultures of the positive clones were grown in LB Kanamycin (30 μg/mL) media and plasmid DNA was extracted using the QIAprep Spin Miniprep Kit (Qiagen). Purified plasmid DNA was sequenced using ABI BigDye Terminator v3.1 Sequencing at the Australian with the following primers ...
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bioRxiv - Molecular Biology 2024Quote: ... ChIP enrichments were analysed by qPCR using the QuantiNova SYBR Green PCR kit (Qiagen; 208054).
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bioRxiv - Developmental Biology 2024Quote: ... RNA was extracted using RNeasy plus mini kit (Qiagen, 74136) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Dneasy Power Soil Kit (Qiagen GmbH, Hilden, Germany), following the manufacturers protocol with some modifications (i.e. ...
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bioRxiv - Neuroscience 2024Quote: ... RNA was extracted with RNeasy Micro Kit (QIAGEN, Germantown, MD, USA) according to manufacturer instructions ...
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bioRxiv - Neuroscience 2024Quote: ... QIAfilter Plasmid kits (Midi prep kit; Qiagen; catalog no.: 12243) were utilized to purify plasmid DNAs following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... RNA was extracted from homogenized deep gray tissue with a QIAsymphony RNA kit (QIAGEN) using the RNA CT 400 v7 protocol ...
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bioRxiv - Cell Biology 2024Quote: ... purified and treated with DNAse I using RNAeasy mini Kit (Qiagen). After quality evaluation by Experion kit and quantification by Qubit ...
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bioRxiv - Developmental Biology 2024Quote: ... 1.5 µg of gDNA was eluted in 15 µl of elution buffer from the EpiTect Fast DNA Bisulfite kit (Qiagen, 59824). A 20 µl PCR reaction was composed of 0.5 µl of bisulfite-converted gDNA input ...
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bioRxiv - Developmental Biology 2024Quote: Genomic DNA (gDNA) was extracted from whole embryos using the Gentra Puregene Tissue kit (Qiagen, 15063), according to the manufacturers’ specifications with one modification ...
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bioRxiv - Cell Biology 2024Quote: ... which was performed using the manufacturer’s protocol (#12123, Qiagen Plasmid Minikit). Isolated plasmid was then sent for Sanger Sequencing to confirm the insertion of point mutations at the desired location and then used for further transfection experiments.
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bioRxiv - Developmental Biology 2024Quote: ... and purified using the MinElute Cleanup kit (Qiagen). pUAST vector was double digested using EcoRI-HF and HpaI (New England Biolabs) ...
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bioRxiv - Cell Biology 2024Quote: ... PA and OA treated (24h and 1week) samples using the RNeasy Micro Kit (Qiagen). Library preparation and sequencing was performed at the Wellcome Trust Sanger Institute (Hinxton ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR products were purified using the MinElute PCR purification kit (Qiagen). Fragments were digested using EcoRI-HF (New England Biolabs ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were sorted directly into the β-mercaptoethanol supplemented RTL Plus buffer of the RNeasy Plus Micro Kit (Qiagen).
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bioRxiv - Developmental Biology 2024Quote: ... the expression vector reported previously (Macfarlan et al., 2012) was isolated from a bacterial extract using the EndoFree Plasmid Maxi Kit (Qiagen #12362), digested with SacII for linearization ...
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bioRxiv - Cell Biology 2024Quote: gDNA was isolated from mature tobacco leaves using the DNeasy® plant Pro Kit (Qiagen). The TRIzolTM reagent (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted from 25 mg of whole kidney tissue by manual homogenization using a 25-gauge needle in RLT buffer as described by the manufacturer (Qiagen RNeasy Mini Kit). The purity of RNA was evaluated using a spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... and purified with MinElute kit (Qiagen). RNA quality was determined using Experion RNA StdSens analysis kit (BioRad ...
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bioRxiv - Cancer Biology 2024Quote: ... Specific siRNA oligonucleotides for β-catenin and control siRNA were obtained from Qiagen, Caveolin-1 ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were digested with proteinase K overnight and DNA was extracted the next day by QIAamp DNA Mini Kit (ref 51306) (Qiagen, USA) with washes performed twice ...
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bioRxiv - Cancer Biology 2024Quote: ... ChIP DNA was purified using Qiagen MinElute Reaction Cleanup kit (Qiagen) following factory protocols.
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bioRxiv - Cancer Biology 2024Quote: ... Tagmented DNA was purified using the MinElute Reaction Cleanup Kit (Qiagen).
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bioRxiv - Cancer Biology 2024Quote: ... Cells were collected at around 80% confluence and snap-freeze on dry ice before batched processed using rNeasy Mini Kit (QIAGEN #74106) as per supplier instructions and eluted in 50 µl nuclease free water (Invitrogen #10977049) ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was isolated using the rNeasy minikit (Qiagen) or with Trizol (Invitrogen) ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was extracted using RNeasy mini kit (Qiagen #74106) including a DNase digestion step according to the manufacturer’s instructions and RNA sequencing was performed as described below ...
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p110α-dependent hepatocyte signaling is critical for liver gene expression and its rewiring in MASLDbioRxiv - Cell Biology 2024Quote: ... The albumin-Cre allele was detected by PCR and HotStar Taq DNA Polymerase (5 U/μl, Qiagen) using the following primers ...
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p110α-dependent hepatocyte signaling is critical for liver gene expression and its rewiring in MASLDbioRxiv - Cell Biology 2024Quote: ... The p110α deletion was confirmed by PCR and HotStar Taq DNA Polymerase (5 U/μl, Qiagen) using two primer pairs ...
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bioRxiv - Molecular Biology 2024Quote: ... we synthesised cDNA using 2 µg of total RNA and cDNA was amplified with a PerfeCTa qPCR fluorescent SYBR Green SuperMix (Quantabio) using real-time quantitative PCR (Rotor-Gene RG 6000— QIAGEN). The primer sequences used for the gene expression are shown in Table S1 ...
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bioRxiv - Molecular Biology 2024Quote: ... The following siRNA were transfected at 100 μM using HiPerFect (Qiagen): ATAD5 (HSS129125) ...
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bioRxiv - Cell Biology 2024Quote: ... Total RNA was extracted using the miRNeasy Micro kit (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: DNA extraction suitable for PacBio SMRT sequencing was performed using the Genomic-tip 100/G (Qiagen Inc) as described previously (Ngabonziza et al. ...
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bioRxiv - Microbiology 2024Quote: ... Cells were resuspended in 500 ul of RLT buffer (Qiagen) with 2-mercaptoethanol and homogenized by bead beating for 3 minute pulses at maximum speed ...
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bioRxiv - Microbiology 2024Quote: ... Approximately 2-4 ml of cells were harvested at each timepoint and incubated with 2 volumes of RNAprotect (Qiagen) at room temperature for 5 minutes prior to centrifugation for 5 minutes at 4000xg 4°C ...
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bioRxiv - Synthetic Biology 2024Quote: ... samples were suspended in a bead tube (Qiagen) along with lysis buffer and loaded on a bead mill homogenizer (Fisherbrand) ...
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bioRxiv - Synthetic Biology 2024Quote: DNA was extracted using the QIAamp PowerFecal Pro DNA kit (Qiagen). Before extraction ...
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bioRxiv - Cell Biology 2024Quote: ... and RNA was extracted using the RNeasy Micro Kit (Qiagen #74004) following the manufacturer’s instructions ...
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Autism genes converge on microtubule biology and RNA-binding proteins during excitatory neurogenesisbioRxiv - Systems Biology 2024Quote: ... DNAs of lentiviral vectors carrying sgRNAs were purified from cells using QIAprep Spin Miniprep kit (Qiagen, Cat#27106) and were Sanger sequenced for sequence validation.
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bioRxiv - Systems Biology 2024Quote: DNA for Nanopore sequencing was purified from frozen samples stored at -80 °C using a Blood & Cell Culture DNA Midi Kit (Qiagen). Approximately 100 mg of tissue sample was crushed in a BioPulveriser cooled in liquid nitrogen ...
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bioRxiv - Plant Biology 2024Quote: Plant total RNA was isolated with the Plant RNeasy kit (Qiagen) including DNase treatment according to the manufacture’s standard protocol ...