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Citations for Qiagen :
451 - 500 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... posterior eye cups (PECs) were separated and collected in 2 mL reinforced tubes (SPEX Sample Prep) containing 5 mM stainless steel beads (Qiagen, LLC).
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bioRxiv - Bioengineering 2024Quote: ... the extraction of total nucleic acid from transfected MOLT-4 cells (small scale, as described) was performed using a DNeasy Blood and Tissue Kit (QIAgen catalog # 69504) which includes direct lysis with proteinase K treatment ...
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bioRxiv - Bioengineering 2024Quote: ... and RNeasy Kit (Qiagen, LLC), respectively ...
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bioRxiv - Bioengineering 2024Quote: ... An RNase step was performed using ribonucleases A (25 ug/ml) (Qiagen) and T1 (25 units/ml ...
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bioRxiv - Bioengineering 2024Quote: ... These expression cultures were set up in 500 uL volumes in deep-well 96-well plates (square wells used for maximum culture aeration; USA Scientific 1896-2800) sealed with porous tape (Qiagen 19571) and incubated at 37°C and 350 RPM for 20 hr ...
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bioRxiv - Microbiology 2024Quote: ... Total RNA was extracted from the pooled samples with the RNeasy mini kit (Qiagen). mRN A purification and fragmentation and the preparation of cDNA libraries were conducted using TruSeq RNA Library Prep Kit v2 kit (Illumina) ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... we used a phenol-chloroform extraction with Qiagen RNAeasy kits (Qiagen, Hilden, Germany) according to the manufacturer instructions ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: All DNA extraction prior to this project was conducted with DNeasy blood and tissue kit (Qiagen, UK). DNA quality and quantity were quantified using a Nanodrop spectrophotometer (2.0 ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Samples were normalized and underwent cDNA synthesis using QuantiTect Reverse Transcription Kits (Qiagen). qPCR was conducted using SYBR green supermix (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... DNA was extracted from the dissected M4 by the QIAamp DNA Mini Kit (Qiagen), and real-time qPCR was performed using primers opcP-F and opcP-R (SI Appendix ...
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bioRxiv - Biochemistry 2024Quote: ... Lysed cells were sedimented by centrifugation at 20,000 RCF at 4 °C for 30 min and the lysate was subsequently loaded onto pre-equilibrated Ni-NTA agarose resin (Qiagen) at 4 °C ...
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bioRxiv - Biochemistry 2024Quote: ... The cell pellet was removed after centrifugation (16000 rpm, 4 °C, 1 h) and supernatant was loaded into a Ni-NTA agarose column (Qiagen). Protein was eluted in an elution buffer (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2024Quote: ... Nickel-nitrilotriacetic acid (Ni-NTA) resin was obtained from Qiagen (Valencia, CA). Stain free gels (4-12% ...
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bioRxiv - Biochemistry 2024Quote: ... The collected elution fraction was reloaded on the Ni-NTA Superflow resin (QIAGEN), with 20 mM imidazole ...
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bioRxiv - Cancer Biology 2024Quote: ... total RNA was isolated from cell pellets using the RNeasy mini kit (Qiagen #74104) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... the RNeasy Mini Kit (QIAGEN) manufacturer’s protocol was strictly followed ...
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bioRxiv - Cancer Biology 2024Quote: ... The mixture was then transferred to MaXtract High Density tubes (QIAGEN, Cat# 129056) and centrifuged at RT for 5 min at 16,000 x g ...
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bioRxiv - Cell Biology 2024Quote: DNA was isolated from ShCtrl and ShOpa1 cells using DNA extraction lysis buffer (Qiagen). The mitochondrial DNA (mtDNA ...
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bioRxiv - Cell Biology 2024Quote: ... The PCR products were purified with QIAquick PCR Purification Kit (QIAGEN) and then submitted for Sanger sequencing ...
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bioRxiv - Cell Biology 2024Quote: ... followed by purification the PCR product using the QIAquick PCR Purification Kit (QIAGEN). Primers for PCR were listed in Supplementary Table3 ...
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bioRxiv - Cancer Biology 2024Quote: ... was conducted using Ingenuity pathway analysis (IPA, QIAGEN, Venlo, Netherlands) and Gene Set Enrichment Analysis (GSEA) ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by the Qiaprep Spin Miniprep Kit (Qiagen). Genomic DNA was isolated via the GeneJet Genomic DNA Purification Kit (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... Precipitated RNA was purified on a “mini-RNA” column (RNeasy Mini Kit from Qiagen), quantified on Nanodrop (Spectrophotometer ND-1000 ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was isolated using RNeasy Mini Kit (Qiagen, Cat. 74104). cDNA was synthesized using a High-Capacity RNA-to-cDNA kit (Vazyme ...
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bioRxiv - Cancer Biology 2024Quote: ... vectors were prepared using the EndoFree-Maxi Kit (Qiagen) and resuspended in a sterile 0.9% NaCl solution/plasmid mix containing 10 μg of pT3-MYC (Addgene #92046) ...
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bioRxiv - Cancer Biology 2024Quote: ... siRNA transfections were performed essentially using Hiperfect reagent (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted via QIAquick PCR Purification Kit (Qiagen, Mansfield, MA). After reverse transcription using iScript cDNA Synthesis Kit (Bio-Rad ...
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bioRxiv - Cancer Biology 2024Quote: ... Human myeloma cell lines were authenticated by subjecting genomic DNA isolated with the QIAamp DNA Mini Kit (Qiagen), and short tandem repeat (STR ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was harvested in QIAZOL and prepared using the Qiagen miRNEASY Kit with DNase On-column digestion (Qiagen, Hilden, Germany), according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was isolated using a Qiagen RNeasy Plus Mini Kit (Qiagen, Hilden, Germany) Cat ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell lysates were further transferred to liquid N2/ -80 °C freezer before RNA purification according to Qiagen protocol (www.Qiagen.com ...
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bioRxiv - Cancer Biology 2024Quote: ... and cells were washed once with PBS (37 °C) and 350 µl RNA lysis buffer (Qiagen, Buffer RLT
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bioRxiv - Cell Biology 2024Quote: ... DNA was harvested from clonal lines and extracted using DNeasy Blood & Tissue kit (Qiagen) and a PCR was run to amplify the C-terminal end of REG1A ...
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bioRxiv - Cell Biology 2024Quote: ... 20,000 events/cells were sorted directly into RNA lysis buffer and RNA was extracted using RNeay Micro Kit (Qiagen). cDNA synthesis and qPCR were set up as described above ...
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bioRxiv - Cell Biology 2024Quote: ... DNA was extracted from the agarose gel using QIAquick Gel Extraction Kit (Qiagen) and send for Sanger sequencing to verify in-frame insertion of the mNeonGreen construct ...
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bioRxiv - Cell Biology 2024Quote: ... 500ng total RNA were transcribed into complementary DNA using oligo-dT primers and OmniScript® Reverse Transcription kit (QIAGEN, Hilden, Germany). Transcription levels of genes of interest were assessed by quantitative PCR (qPCR ...
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bioRxiv - Cell Biology 2024Quote: ... utilizing the RNeasy Plus Mini kit (Qiagen, Hilden, Germany). Reverse transcription was carried out using the iScript cDNA Synthesis Kit (BIO-RAD ...
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bioRxiv - Cell Biology 2024Quote: ... and RNA was extracted using RNeasy Mini Kit (Qiagen #74104) according to manufacturer instructions ...
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bioRxiv - Cell Biology 2024Quote: DNA was extracted from 200 µL of the resuspended extracellular vesicles using the DNeasy Blood & Tissue Kit (Qiagen, cat# 69504) according to the instructions in the Purification of Total DNA from Animal Blood or Cells (Spin-Column Protocol) ...
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bioRxiv - Cell Biology 2024Quote: ... Total RNA quantity was measured using nanodrop and qRT-PCR assays were performed using QuantiNova™ SYBR Green RT-PCR Kit (Qiagen, Cat.# ID: 208154) in StepOnePlus™ Real-Time PCR System by Applied biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... The linearised plasmid was run on agarose gel and purified with QIAquick Gel Extraction Kit (Qiagen; #28704). sgRNA oligonucleotides (TAG Copenhagen AS ...
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bioRxiv - Cell Biology 2024Quote: ... The band containing the T7 promoter and sgRNA template was cut from the agarose gel and purified with QIAquick Gel Extraction Kit (Qiagen; #28704). This linear DNA template containing the T7 promoter was used to transcribe sgRNAs with the MEGAshortscript™ T7 Transcription Kit (Invitrogen ...
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bioRxiv - Biophysics 2024Quote: ... The complex was initially screened by the following commercial crystallization screens: Nucleix (Qiagen) and Wizard I&II (Rigaku reagents) ...
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bioRxiv - Biophysics 2024Quote: ... or anti-His6X (His HRP conjugate by Qiagen) antibodies at a 1:1,000 dilution ...
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bioRxiv - Biophysics 2024Quote: ... Plasmid DNA isolation was performed using the QIAprep Spin Miniprep kit (Qiagen, #27104) following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2024Quote: ... where the band corresponding to the desired handle length (∼1.6 kb or 1.7 kb for LH and RH respectively) was excised and then the DNA extracted using a gel-extraction kit (Qiagen). The RNA hairpin substrate was then annealed and ligated between the LH and RH handles (see final construct configuration as in Fig ...
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bioRxiv - Cancer Biology 2024Quote: ... mRNA expression levels were measured with the RT2 Profiler PCR Array with a custom designed panel (QIAGEN, 3445261) using the Power SYBR™ Green PCR Master Mix (QIAGEN ...
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bioRxiv - Cancer Biology 2024Quote: ... using the Power SYBR™ Green PCR Master Mix (QIAGEN, 4367659) according to manufactureŕs instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were washed with PBS and lysed with 350 μl RLT Plus from the RNeasy Plus Mini Kit (QIAGEN, 74136) before RNA isolation following manufactureŕs instructions ...
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bioRxiv - Cancer Biology 2024Quote: By utilizing the RNeasy Mini Kit (Qiagen), RNA extraction was based on the manufacturer’s protocol ...