Labshake search
Citations for Qiagen :
401 - 450 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... The aqueous phase was then mixed with an equal volume of 70% ethanol and transferred to a silica column from the RNeasy Mini kit (Qiagen). Total RNAs were then purified according to the instructions of the manufacturer ...
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bioRxiv - Immunology 2024Quote: ... DNA was then extracted using a DNeasy Blood & Tissue Kit (Qiagen, Germantown, MD) according to the instructions ...
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bioRxiv - Genomics 2024Quote: Genomic DNA was extracted from blood using a MagAttract Blood DNA/RNA Kit (Qiagen, Venlo, Netherlands) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... and with MagAttract HMW DNA kit (Qiagen, Germantown, MD, USA) for Illumina ...
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bioRxiv - Immunology 2024Quote: ... and DNase I (QIAGEN Cat. No. 79254) treatments ...
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bioRxiv - Immunology 2024Quote: ... Amplified target DNA was purified using QIAquick Gel Extraction Kit (QIAGEN Cat. No. 28704) and sent to the Arizona State University’s Genomics Core for Sanger sequencing.
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bioRxiv - Immunology 2024Quote: ... Ingenuity Pathway Analysis (Qiagen) was also performed using differentially expressed genes.
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bioRxiv - Immunology 2024Quote: ... RNA was isolated using the RNeasy mini kit (Qiagen, Germantown, MD), and gene expression was studied using the nCounter mouse myeloid cell panel with probes for 734 genes and 20 internal reference genes (Nanostring Technologies ...
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bioRxiv - Immunology 2024Quote: ... The manufacturer’s instructions were used to extract DNA and RNA using RNase A (QIAGEN Cat. No. 19101) and DNase I (QIAGEN Cat ...
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bioRxiv - Genomics 2024Quote: ... 5uL of a second strand synthesis mix consisting of 60% elution buffer (Qiagen), 27% second strand synthesis buffer (NEB) ...
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bioRxiv - Genetics 2024Quote: Total genomic DNA was extracted from 10% suspension of fin clips (<1 mm2) using the DNeasy Blood and Tissue Kit (Qiagen) according to the manufacturer’s protocols ...
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bioRxiv - Genetics 2024Quote: ... The resulting PCR product was purified using the QIAquick PCR purification kit (QIAGEN, Hilden, Germany) and sequenced to confirm the identity (S3) ...
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bioRxiv - Genetics 2024Quote: ... and treated with DNase I (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... using the Qiagen RNeasy mini kit (Qiagen, Hilden, Germany) and treated with DNase I (Qiagen ...
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bioRxiv - Genetics 2024Quote: ... and proteinase K was inactivated at 95°C for five minutes.39 PCR was performed on isolated genomic DNA followed by analysis on a QIAxcel Advanced System instrument using capillary electrophoresis (Qiagen). Primer3plus45 was used to design primers and oligonucleotides obtained from IDT (Supplementary Table 1).
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bioRxiv - Genetics 2024Quote: ... RNA extracted using RNeasy (Qiagen), followed by reverse transcription with Superscript III (Invitrogen) ...
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bioRxiv - Genetics 2024Quote: ... The master mix was composed of 10 µL 2x QuantiTect Multiplex PCR NoROX reagent (Qiagen), 0.5 µM of each primer and 2 µL DNA template ...
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bioRxiv - Genetics 2024Quote: ... The 2nd-round PCR product was purified using the QIAquick PCR purification kit (QIAGEN, Hilden, Germany), sequenced and submitted for Synthego Inference of CRISPR Edits Analysis (https://ice.synthego.com/#/) ...
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bioRxiv - Genetics 2024Quote: ... and amplicons of expected lengths were excised and extracted with the QIAquick Gel Extraction Kit (Qiagen). For sequencing ...
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bioRxiv - Genetics 2024Quote: ... DNA was extracted in randomized blocks using the DNeasy Blood & Tissue kit (Qiagen, Catalog # 69506), purified with the Zymo DNA cleanup kit (Catalog # D4064) ...
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bioRxiv - Genetics 2024Quote: ... qRT-PCR was performed in a 10 μL volume using the miRCURY LNA SYBR Green PCR Kit (Qiagen, Hilden, Germany) and the following miRCURY LNA miRNA PCR primer sets (Qiagen ...
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bioRxiv - Genetics 2024Quote: ... was isolated with the miRNeasy Mini Kit (Qiagen, Hilden, Germany) following the animal tissue protocol according to manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... The PCR reactions were performed using Taq DNA polymerase (Qiagen) with the Taq PCR buffer ...
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bioRxiv - Genetics 2024Quote: ... and the following miRCURY LNA miRNA PCR primer sets (Qiagen, Hilden, Germany): dme-miR-210-3p (YP02104327) ...
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bioRxiv - Genomics 2024Quote: ... We then gel purified reactions with the QIAquick PCR & Gel Cleanup Kit (Qiagen 28506). Libraries were quantified with the Qubit High Sensitivity dsDNA Quantitation assay (Thermo Fisher Scientific Q32854 ...
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bioRxiv - Genomics 2024Quote: The DNA of the 80 used for GBS was isolated using the spin-column DNeasy plant mini kit (Qiagen USA, Valencia, CA) (Qiagen) ...
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bioRxiv - Genomics 2024Quote: ... and then eluted in 30 µL of EB buffer (MinElute PCR Purification Kit, QIAGEN). The step of the adapter fill-in was performed using 13 µL of water ...
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bioRxiv - Genomics 2024Quote: The DNA of the 80 used for GBS was isolated using the spin-column DNeasy plant mini kit (Qiagen USA, Valencia, CA) (Qiagen). For the other 259 samples ...
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bioRxiv - Genomics 2024Quote: ... 1 mL of PE buffer and 100 µL of EB buffer (MinElute PCR Purification Kit, QIAGEN). The silica columns were placed in a collection tube to dry and later in a 1.5 mL DNA lo-bind tube (Eppendorf ...
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bioRxiv - Genomics 2024Quote: ... the samples were purified in 35 µL of EB buffer (MinElute PCR Purification Kit, QIAGEN). To measure the concentration of dsDNA/sequencing libraries and to confirm that library preparation was successful ...
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bioRxiv - Genomics 2024Quote: ... RNA was depleted of rRNA using the QIAseq FastSelect RNA Removal Kit (Qiagen). RNA-seq libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Genomics 2024Quote: ... The transposed DNA was then purified using the MinElute Kit (Qiagen), eluted with 10.5 μl elution buffer (EB) ...
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bioRxiv - Genetics 2024Quote: ... Genomic DNA was extracted from mixed stage growing populations using reagents from the Puregene Core Kit A (QIAGEN, Valencia, CA) and quantified using Qubit 3.0 fluorometer (Life Technologies ...
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bioRxiv - Genomics 2024Quote: ... with the automated extraction platform QIAcube (Qiagen).
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bioRxiv - Genomics 2024Quote: ... the effect of human rRNA removal was also assessed using a QIAseq FastSelect rRNA removal kit (Qiagen). The rRNA removal reaction was performed after RNA thermal denaturation at 95℃ 5 min according to the instructions.
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bioRxiv - Genetics 2024Quote: ... Pathway enrichment analyses were conducted with QIAGEN Ingenuity Pathway Analysis (IPA) (QIAGEN Inc. ...
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bioRxiv - Genetics 2024Quote: ... After purification (Qiagen QIAquick) the PCR product was sequenced with primers P3’Fw2 and/or P3’Rev2 ...
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bioRxiv - Genetics 2024Quote: ... embedded in the optimum cutting temperature (OCT) compound Tissue-Tek (Qiagen, Hilden, Germany) and frozen in liquid nitrogen ...
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bioRxiv - Genetics 2024Quote: ... before being processed with the DNeasy Plant Mini Kit from Qiagen.
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bioRxiv - Genetics 2024Quote: miR-210 expression levels in both flies and mice were quantified by quantitative real-time PCR (qRT-PCR) using the miRCURY LNA miRNA PCR assay (Qiagen, Hilden, Germany). First-strand cDNA synthesis was obtained from 10 ng of total RNA by using the miRCURY LNA RT Kit (Qiagen ...
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bioRxiv - Genetics 2024Quote: ... First-strand cDNA synthesis was obtained from 10 ng of total RNA by using the miRCURY LNA RT Kit (Qiagen, Hilden, Germany) following manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... cDNA was diluted to 5 ng/µl in RNase-free water and 1 µl used for real-time qPCR with Qiagen QuantiNova SYBR Green Master Mix (Qiagen) in a StepOnePlus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Genomics 2024Quote: ... Poly(A)RNA (500 ng) from each independent transfection was reverse transcribed to cDNA using QuantiTech Reverse Transcription kit (Qiagen) using a reporter-specific first strand primer (HJ7211 ...
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bioRxiv - Genomics 2024Quote: ... followed by repeated elution with the entire volume of the original eluate (Qiagen, Germantown, MD). RNA concentration and quality were determined using Agilent TapeStation (Agilent Technologies).
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bioRxiv - Genomics 2024Quote: ... 72 °C for 1 min and then subjected to a 1.2x SPRI cleanup eluting in 42 µl EB buffer (Qiagen). Each sample was split into two fractions and each of them was further amplified with modality-specific primers ...
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bioRxiv - Genomics 2024Quote: ... and mRNA treated with gDNA wipeout buffer (Qiagen) before preparing sequencing libraries ...
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bioRxiv - Genomics 2024Quote: ... RNA was extracted using the RNeasy Mini Kit (Qiagen, Hilden, Germany), following the manufacturer’s instructions.
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bioRxiv - Genomics 2024Quote: ... Samples from 2016-2017-2018-2019 (n=1,011) were extracted from dried blood spots using the QIAamp DNA mini kit according to the manufacturer’s instructions (Qiagen, Hilden, Germany). For samples from 2020-2021 (n=716) ...
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bioRxiv - Genomics 2024Quote: DNA was extracted from 91 P21- and 88 C1-infected animals using a Qiagen Genomic Tips kit (Qiagen ID: 10223). The P21 DNA sample was sent to Genomic Facility of ETH D-BSSE (Basel ...
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bioRxiv - Genomics 2024Quote: ... were heat killed in a 95°C water bath for 20 min to produce thermolysates before DNA extraction (MagAttract kit, Qiagen) and whole-genome sequencing ...