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Citations for Qiagen :
101 - 150 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The pellets were resuspended with OMV buffer and re-pelleted again by centrifugation at 100,000 g (31,000 RPM) for 2 h at 4°C and lysed with Qiazol followed by RNA isolation with the miRNeasy kit (Qiagen, Germantown, MD) to obtain total RNA including the small RNA fraction ...
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bioRxiv - Microbiology 2024Quote: ... run using the QIAcuity One Digital PCR System (Qiagen, 2-plex Device, Cat. No. 911001). The following conditions were used for the one-step cycling dPCR program ...
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bioRxiv - Microbiology 2024Quote: ... Amplification and detection were performed using the QIAcuityTM OneStep Advanced Probe Kit (Qiagen, Cat. No. 250132), with primers and probe targeting the influenza A virus M gene segment (specific amplicon length is 110 bp) ...
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bioRxiv - Microbiology 2024Quote: ... followed by purification and DNase treatment (RNeasy kit, RNase-Free DNase Set, Qiagen). RNA was submitted for 150 bp paired-end sequencing on an Illumina HiSeq platform (Azenta Life Sciences) ...
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bioRxiv - Microbiology 2024Quote: ... the reactions were loaded into QIAcuityTM Nanoplate 8.5k 96-well (Qiagen, Cat. No. 250021) run using the QIAcuity One Digital PCR System (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... mosquitoes were homogenized in 500 μl ice-cold 1X Phosphate Buffered Saline buffer with two ice-cold steel bearing balls (3 mm diameter, LOUDET) using a TissueLyser II (Qiagen) and clarified through centrifugation ...
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bioRxiv - Microbiology 2024Quote: ... and genomic DNA extracted using the DNeasy Kit (Qiagen, Hilden, Germany). Genome was sequenced using a Hi Seq 2000 (Illumina Inc. ...
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bioRxiv - Microbiology 2024Quote: RNA extraction was performed using the QIAamp Viral RNA Mini extraction kit (Qiagen, Cat. No. 52906) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... using the QIAamp DNA Mini Kit (Qiagen, Germantown, MD) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... reverse transcription quantitative PCR (RT-qPCR) assays were performed with the QuantiTect Virus + ROX Vial Kit (Qiagen, Toronto, ON, Canada) in a LightCycler® 480 system (Roche Molecular System) ...
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bioRxiv - Cell Biology 2024Quote: ... DNase (Qiagen) treatment was performed to remove residual DNA ...
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bioRxiv - Molecular Biology 2024Quote: RNA was isolated using QIAzol Lysis Reagent and the Qiagen RNeasy kit (Qiagen, Hilden, Germany). Complementary DNA (cDNA ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA pellets were washed 3X with 70% ethanol and hydrated in pre-warmed (37°C) Elution buffer (Qiagen, 10 mM Tris-Cl, pH 8.5. Cat. 19086) and incubated on HulaMixer™ Sample Mixer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... resuspended in 600 µL of RLT buffer (RNeasy Mini kit, 74104, Qiagen) and stored at -80°C until subsequent RNA isolation ...
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bioRxiv - Microbiology 2024Quote: Cells were grown to 40% confluence and then incubated with 50 nM siRNA and Attractene transfection reagent (QIAGEN) for 36 h according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... RNA was isolated using the RNeasy Mini kit (74104, Qiagen). Libraries were prepared using the TruSeq® Stranded mRNA Library Prep (20020594 ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysed cell material was stored overnight at −80 °C before total RNA was isolated using the RNeasy Plus Mini Kit (Qiagen). RNA isolation and RT-qPCR was performed by the University of Rochester Genomics Research Center ...
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bioRxiv - Microbiology 2024Quote: ... and an additional cleaning step running the final volume through an RNA extraction kit column (Qiagen).
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bioRxiv - Microbiology 2024Quote: ... The DNeasy® PowerFood® Microbial Kit (QIAGEN, Germany) was used following the manufacturer’s instructions with minor exceptions ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were eluted in 40 ml of elution buffer (Qiagen, 10 mM Tris-Cl, pH 8.5) with optional 15 mins of incubation at 37°C to recover long molecules ...
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bioRxiv - Molecular Biology 2024Quote: ... 2x Elution buffer (Qiagen, 10 mM Tris-Cl, pH 8.5), 1x rCutsmart Buffer.
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bioRxiv - Immunology 2024Quote: Total RNA was extracted from homogenized ileal and colonic tissues by use of the RNeasy Mini kit (Qiagen, Valencia, CA) and reverse transcribed (High-Capacity cDNA Reverse Transcription Kit ...
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bioRxiv - Immunology 2024Quote: ... Data analysis was performed using Ingenuity Pathway Analysis (IPA; www.ingenuity.com, Qiagen, CA, USA) with the Core Analysis module to identify significant canonical pathways affected between Th9WT and Th9KO cells ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting plasmid was then transformed into E.coli DH10b strain for isolation via QIAprep Plasmid Mini-Prep Kit (Qiagen). The isolated plasmid was subsequently transformed into D.radiodurans R1 chemically competent cells (above) ...
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bioRxiv - Molecular Biology 2024Quote: ... Genomic DNA was extracted using QIAamp blood mini-kits (Qiagen GmbH, Hilden, Germany) according to the manufacturer’s instructions and stored at 4°C before use ...
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bioRxiv - Molecular Biology 2024Quote: ... All fragments were then gel-purified using QIAquick Gel Extraction Kit (Qiagen) and assembled with HindIII-digested pUC19mPheS plasmid using NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs Inc.) ...
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bioRxiv - Cancer Biology 2024Quote: ... along with a pre-chilled 5-mm steel bead (QIAGEN). All samples were then placed in pre-chilled cassettes for the Tissue Lyser II and pulverized for at 30 1/s for three 1-minute oscillations ...
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bioRxiv - Molecular Biology 2024Quote: ... and 10 μl of Quantifast SYBR Green PCR Master Mix (QIAGEN) or PowerUp™ SYBR™ Green Master Mix (ThermoFisher ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA isolation from 200 mg of grounded tissue was performed with the RNeasy plant mini kit (Qiagen, Hilden, Germany) and the cDNA synthesis with the First Strand cDNA Synthesis Kit (Thermo Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Viable (PI-) single cells were sorted into 2.5µL of RLT PLUS buffer (Qiagen) containing 2.5U SUPERas-In (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by miRNeasy mini column (Qiagen). cDNA was generated from 1-2 mg of total RNA using random hexamers and Maxima Reverse Transcriptase (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... using the QuantiFast SYBR Green PCR Kit (Qiagen). Thermocycling was done for 40 cycles in a two-step cycling in accordance with the manufacturer’s instructions and each PCR reaction was performed in triplicate ...
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bioRxiv - Molecular Biology 2024Quote: ... and the DNA was purified using a QIAfilter Plasmid Midi Kit (Qiagen, 12243). Biotin-containing handles were made using a PCR on pBluescript SK+ (Stratagene ...
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bioRxiv - Microbiology 2024Quote: ... with a Qiacube (Qiagen Canada). The extracted DNA was dissolved in 50 µ L water ...
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bioRxiv - Molecular Biology 2024Quote: ... and Total RNA was isolated using miRNeasy mini kit (QIAGEN, Cat# 217004) according to the manufacturer’s protocol and as previously described22,65 ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were lysed with QIAzol lysis reagent (QIAGEN, Cat# 79306), and Total RNA was isolated using miRNeasy mini kit (QIAGEN ...
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bioRxiv - Microbiology 2024Quote: ... One hundred microliters (uL) PBS was added to NW pellets followed by 280uL AVL lysis buffer (Qiagen, Germantown, MD, USA). Two to three mL blood was collected from the cranial vena cava in an SST tube for serum separation ...
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bioRxiv - Microbiology 2024Quote: ... Carrier RNA was added to each sample and RNA was extracted using EZ1 DSP kit on a Qiagen EZ1 Advanced XL extractor according to the manufacturer’s instructions (Qiagen, Germantown, MD, USA). RNA was eluted in 120 uL RNase-free water and stored at -80°C until evaluated for gene expression by qRT-PCR ...
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bioRxiv - Microbiology 2024Quote: DNA was extracted from cryopreserved stool and colon biopsies using the PowerFecal DNA Isolation Kit and the company’s recommended protocol (Qiagen, Valencia, CA). Sequencing libraries were prepared as described by the Earth Microbiome Project [53] and sequenced on an Illumina MiSeq Sequencer (Illumina ...
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bioRxiv - Cell Biology 2024Quote: ... DNA fragments ranging from 350 to 700 bp were excised from the gel and purified using a QIAGEN gel extraction kit (QIAGEN). PCR and library preparation were conducted as previously described25 ...
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bioRxiv - Cell Biology 2024Quote: ... or QIAamp DNA Blood Midi Kit (QIAGEN). The purified DNA was digested overnight with SbfI-HF (New England Biolabs ...
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bioRxiv - Microbiology 2024Quote: ... To optimize the DNA extraction the supernatant was taken off and digested with RNAse A (Qiagen). The DNA was then automatically purified with the QIAamp 96 QIAcube HT kit (Qiagen #51331 ...
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bioRxiv - Microbiology 2024Quote: ... The DNA was then automatically purified with the QIAamp 96 QIAcube HT kit (Qiagen #51331) with additional proteinase K on a QIAcube HT following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: RNA was isolated from RPE-1 cells using the RNeasy mini kit (Qiagen, CA) and cDNA was generated using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Microbiology 2024Quote: ... and extracted twice in stool lysis buffer ASL (Qiagen, Hilden Germany). Bead beating at a speed of 5 m/s for 60 seconds (x2 ...
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bioRxiv - Microbiology 2024Quote: ... and purified using QIAamp DNA Mini Kit (Qiagen). The purified DNA was tested for purity with a nanophotometer (Implen NP80 ...
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bioRxiv - Microbiology 2024Quote: ... Swabs were DNA extracted using a DNeasy blood and tissue kit (Qiagen). Living frogs were weighed at day 30 and day 60 to estimate health and body condition.
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bioRxiv - Microbiology 2024Quote: ... The QIAseq miRNA Library Kit (Qiagen) was used to prepare cDNA libraries ...
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bioRxiv - Cell Biology 2024Quote: ... Gel slices corresponding to fragments approximately 147 - 160 bp in size were then extracted using the MinElute Gel Extraction Kit (Qiagen, #28604). Libraries were quantified with Qubit dsDNA HS Kit (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was then cleaned up using a MinElute Reaction CleanUp Kit (Qiagen). To reduce adaptor dimers ...