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Citations for Qiagen :
101 - 150 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... or a RNeasy Micro kit (74004, Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Complementary DNA (cDNA) synthesis was performed using the QuantiTect Reverse Transcription Kit (205313, Qiagen). qRT-PCR was performed on a QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Developmental Biology 2024Quote: ... and purified with the RNeasy MinElute Cleanup Kit (QIAGEN). Approximately 5 to 10 pl of capped mRNAs were microinjected into the cytoplasm of oocytes using a micromanipulator (IM-300 microinjector ...
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bioRxiv - Developmental Biology 2024Quote: ... two independent limbs were microdissected and snap-frozen at −80°C for subsequent total RNA extraction using the RNEasy Mini Kit (QIAGEN, 74134) following protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA extraction was carried out with the RNEasy Micro Kit (QIAGEN, 74004) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... and reverse-transcribed using the Quantitect Reverse Transcription Kit (Qiagen # 205311) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... Digested chromatin fragments were then purified using the MinElute PCR Purification Kit (Qiagen), according to manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... After being subjected to PCR clean-up (Qiagen), the UTR-library amplicons were then appended with EGFP CDS (derived from EGFP-N1 vector ...
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bioRxiv - Genetics 2024Quote: ... The PCR product was purified again by QIAGEN PCR Purification kit for Illumina® NextSeq paired-end 150 sequencing.
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bioRxiv - Genetics 2024Quote: ... After subjected to PCR clean-up (Qiagen), the UTR-library amplicons were then appended with EGFP CDS and CMV promoter sequence (derived from EGFP-N1 vector ...
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bioRxiv - Genetics 2024Quote: ... separation of the samples was followed by total RNA extraction using the QIAGEN RNeasy Mini Kit (Qiagen catalog number 74104) with on-column DNase I digestion using RQ1 RNase-Free DNase (Promega catalog number M6101).
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bioRxiv - Evolutionary Biology 2024Quote: ... The sampled cellular mass from each clone was transferred into a lysis buffer (DNeasy® Plant Mini Kit, QIAGEN), and cells were disrupted by 1.5-min vortexing (Beadbeater ...
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bioRxiv - Developmental Biology 2024Quote: Genomic DNA (gDNA) from >1M NPC was extracted using column-based DNeasy Blood & Tissue Kit (Qiagen). DNA integrity was tested on a 0.8% agarose gel and high-quality gDNA was used to prepare libraries according to the NEBNext® Enzymatic Methyl-seq Kit following the section for large insert libraries with minor modifications ...
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bioRxiv - Developmental Biology 2024Quote: ... extracted using the MinElute Gel Extraction Kit (Qiagen) according to the manufacturer’s protocol and sequenced by Genewiz (Azenta Life Sciences) ...
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bioRxiv - Developmental Biology 2024Quote: ... These were cloned into Qiagen’s pDrive cloning vector using the Qiagen PCR Cloning Kit (Qiagen) and clones verified by sequencing (Department of Biochemistry Sequencing Facility ...
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bioRxiv - Developmental Biology 2024Quote: RNA was extracted from > 1M NPCs using the RNeasy kit and on-column DNAse digestion (Qiagen). RNA integrity was measured using the Bioanalyzer Nano Kit ...
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bioRxiv - Developmental Biology 2024Quote: ... thresholds were used to prepare Reduced Representation Bisulfite Sequencing (RRBS) libraries using the Ovation RRBS Methyl-Seq System 1-16 (NuGen) and the EpiTect Fast DNA Bisulfite kit (Qiagen). Libraries were sequenced using the NextSeq500 platform (Illumina ...
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bioRxiv - Developmental Biology 2024Quote: ... Genomic DNA was extracted using the AllPrep DNA/RNA/miRNA Universal Kit (Qiagen). Samples which met quantity (11.75ng/µL ...
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bioRxiv - Developmental Biology 2024Quote: RNA extraction was conducted using the RNeasy Mini Kit from Qiagen following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA extraction was performed with the QIAGEN RNeasy Micro Kit (QIAGEN, Hilden, Germany). RNA concentration after extraction was assessed by using the NanoDrop® ND-1000 UV-Vis Spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... purification was carried out using mini elute PCR Purification Kit (Qiagen, Cat# 27104). The appropriate number of amplification cycles was determined using qPCR reaction as described63 ...
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bioRxiv - Developmental Biology 2024Quote: ... resuspended in 350 μl RLT buffer (QIAGEN) and homogenized using a 1 ml syringe with a 21G x 1½” needle ...
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bioRxiv - Developmental Biology 2024Quote: ... Total RNA was extracted from pools of 20-25 embryos (unless otherwise stated) using either TRIzol chloroform extraction followed by isopropanol precipitation and LiCl precipitation or using the RNeasy Mini Kit (QIAGEN). 1 µg of RNA was then used to synthesize cDNA in a 20 µl reverse transcription reaction using 5X First Strand Buffer and MMLV-RT (Promega ...
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bioRxiv - Developmental Biology 2024Quote: ... Expression plasmids were transfected using Effectene transfection reagent (QIAGEN). Plasmids were generated via Gateway® technology (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were then subject to automated RNA extraction using the RNeasy Mini Kit (QIAGEN), with in-column DNase treatment step ...
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bioRxiv - Developmental Biology 2024Quote: Ingenuity Pathway Analysis (IPA, version 60467501, QIAGEN Digital Insights) was used to predict activated or inhibited biological functions ...
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bioRxiv - Developmental Biology 2024Quote: ... using QuantiNova SYBR Green PCR Kit (208054, Qiagen). All primer sequences are shown in Supplementary Table 1 ...
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bioRxiv - Developmental Biology 2024Quote: ... and purified using RNeasy Minelute RNA cleanup kit (Qiagen). RNA quality was assessed using microcapillary electrophoresis on Agilent 2100 Bioanalyzer with RNA Pico 6000 kit (Agilent ...
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bioRxiv - Genetics 2024Quote: ... RNA was extracted with QIAzol and RNeasy Mini kit (Qiagen) according to the manufacturer’s recommendations ...
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bioRxiv - Genetics 2024Quote: ... RNA was extracted with RNeasy spin columns and digested with DNase I (Qiagen) for 30 minutes at room temperature followed by a column cleanup with RNeasy spin columns ...
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bioRxiv - Genetics 2024Quote: ... and both the QIAseq FastSelect–5S/16S/23S ribodepletion and FastSelect rRNA Plant reagents (Qiagen, Hilden Germany) in combination ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Genomic DNA was extracted from the lysate using the DNeasy® Plant Mini Kit (QIAGEN) following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and P3c genomic libraries were mapped against the PE-2_H4 genome (GenBank accession number GCA_905220315.1) using the Burrows–Wheeler aligner algorithm implemented by the CLC Genomics Workbench 8.01 (QIAGEN). A cut-off of 0.8 for aligned read length and 0.8 for minimal required identity were set ...
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bioRxiv - Developmental Biology 2024Quote: ... Total RNA was extracted using the RNeasy Plant Mini Kit (QIAGEN) and first-strand cDNA was synthesized with the SuperScript® VILOTM cDNA Synthesis Kit (Invitrogen) ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were transfected using Effectene (Qiagen #301427), incubated for 44–48 hours at 28°C ...
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bioRxiv - Developmental Biology 2024Quote: Whole mouse kidneys were homogenized in lysis buffer (Qiagen) and total RNA was extracted from a portion of the lysate using RNeasy Mini kit ...
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bioRxiv - Developmental Biology 2024Quote: ... The chromatin was purified with Qiaquik PCR purification kit (Qiagen, 28106) and used for next generation sequencing or for quantitative PCR ...
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Notch3 is a genetic modifier of NODAL signalling for patterning asymmetry during mouse heart loopingbioRxiv - Developmental Biology 2024Quote: Reverse transcription was performed on RNA isolated from entire (left and right) micro-dissected heart fields using a Reverse Transcription kit (QuantiTect, Qiagen). Quantitative PCR was carried out using the ViiA7 real-time PCR system ...
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bioRxiv - Developmental Biology 2024Quote: ... morulae (48 h post-IVF) and blastocysts (68 h post-IVF) were collected in 5uL of 1x TCL lysis buffer (Qiagen #1031586) containing 1% (v/v ...
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bioRxiv - Developmental Biology 2024Quote: ... After purification with Qiagen MinElute PCR Purification Kit (Qiagen, 28004), individual libraries were size selected (100–800 bp ...
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bioRxiv - Developmental Biology 2024Quote: ... Faecal DNA extraction was performed using the PowerSoil® DNA Isolation Kit (Qiagen, Clayton, Victoria, Australia) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... which was further verified and purified from gel with a DNA purification kit (Qiagen, Cat.No. 28704). Then ...
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bioRxiv - Developmental Biology 2024Quote: gDNA extractions were done using Qiagen DNeasy Blood & Tissue Kit (Qiagen, Valencia, CA; Cat# 69506). PCR amplification was done using the following primers for targeted region ...
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bioRxiv - Developmental Biology 2024Quote: ... the final plasmids were isolated and purified using the Qiagen Plasmid Maxi kit (QIAGEN, Cat.No.12162). Subsequently ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Batches of samples were processed in parallel to collect RNA with Qiagen RNeasy extraction kits (Qiagen 74034). After isolating total RNA ...
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bioRxiv - Evolutionary Biology 2024Quote: ... total RNA was extracted using Trizol (Qiagen) from i5 males (6-8 biological replicates per treatment ...
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bioRxiv - Developmental Biology 2024Quote: ... GFP-positive cells were sorted into REPLI-g cell storage buffer (Qiagen).
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bioRxiv - Developmental Biology 2024Quote: Genomic DNA from freshly sorted single cells was extracted and amplified using the REPLI-g Advanced Single Cell kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: Pools of 5 embryos were used for RNA extraction via the Qiagen RNeasy Micro Kit (Qiagen, Valencia CA; Cat# 74004). RNA was eluted in RNase-free water and sent to the New York University Genome Technology Center for RNA-sequencing ...
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bioRxiv - Developmental Biology 2024Quote: Total RNA was extracted using the RNeasy plus Mini kit (QIAGEN, Cat.No. A25776). The quality of the RNA was measured using the Agilent 2100 Bioanalyser with the Agilent RNA 6000 Nano kit (Agilent ...