Labshake search
Citations for Qiagen :
251 - 300 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: Total RNA (totRNA) from the roots was extracted with an RNeasy Plant Mini Kit (Qiagen Inc., Valencia, CA) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... The cDNA was then synthesized with an Omniscript Reverse Transcription (RT) Kit (QIAGEN). Quantitative PCR reactions were carried out in a CFX Connect Real-time PCR detection system (BioRad ...
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bioRxiv - Synthetic Biology 2024Quote: DNA extraction (DNeasy blood & tissue kit, QIAGEN) and library preparation procedures were carried out as previously described in (Herz et al ...
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bioRxiv - Plant Biology 2024Quote: ... Removal of the DNA from the samples was carried out through a DNase I treatment (QIAGEN). The cDNA was then synthesized with an Omniscript Reverse Transcription (RT ...
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmid DNA was extracted using the QIAprep Spin Miniprep Kit (QIAGEN) and constructs were validated by Sanger sequencing (Eurofins Genomics ...
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bioRxiv - Synthetic Biology 2024Quote: ... Samples were prepared in duplicates in a standard 96-well PCR plate and transferred to a QIAcuity 8.5k 96-well Nanoplate (Qiagen) for partitioning using the Qiagen Standard Priming Profile ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 34 through Qiacuity digital PCR (Qiagen, 250131).
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bioRxiv - Synthetic Biology 2024Quote: ... Digital PCR for dCas was conducted on a QIAcuity Digital PCR system (Qiagen). RNA was prepared according to manufacturer’s protocol using the QIAcuity OneStep Advanced Probe kit ...
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bioRxiv - Synthetic Biology 2024Quote: ... Tissue was homogenized using metal PowerBead tubes and QIAshredder columns (Qiagen). Total RNA was extracted from the homogenate using the PureLink RNA Mini kit (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... homogenized using a tissue lyser (Qiagen, Germany) and the proteins were isolated using an extraction buffer contenting 50 mM Tris-HCl (pH 7.5) ...
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Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neuronsbioRxiv - Neuroscience 2024Quote: ... Total RNA was extracted from fresh frozen cell pellet samples using Qiagen RNeasy Plus Universal mini kit following manufacturer’s instructions (Qiagen, Hilden, Germany). RNA samples were quantified using Qubit 2.0 Fluorometer (Life Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was isolated using the Allprep DNA/RNA Micro Kit (Qiagen) per manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2024Quote: ... and RNA was extracted using RNeasy Kits (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... genomic DNA was isolated from spleens of reconstituted lymphoma bearing mice (containing malignant and normal hematopoietic cells) using DNeasy Blood and Tissue Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and plasmid was maxiprepped (Qiagen); the libraries were sequenced as described below to confirm even representation of guides ...
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bioRxiv - Biochemistry 2024Quote: ... 0.1 mg/ml RNase A (QIAGEN), and 10% glycerol ...
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bioRxiv - Biochemistry 2024Quote: RNA was extracted from MEF cells using the RNeasy Mini Kit (Qiagen) following manufacturer instructions ...
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bioRxiv - Biochemistry 2024Quote: ... Initial screening was conducted using the sitting-drop vapor diffusion method with the ARI Crystal Gryphon Robot (ARI) and crystallization screening kits (QIAGEN) at 18°C ...
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bioRxiv - Biochemistry 2024Quote: ... and 0.1 mg/ml RNase A (QIAGEN)] ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... the samples were homogenized using a TissueLyser II (Qiagen) with a mixture of 2.8 mm ceramic (VWR) ...
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bioRxiv - Bioengineering 2024Quote: ... and was then subjected to Ni-NTA affinity purification at 4 [(Qiagen, Valencia, CA). The column was adequately washed with 20 column volume of wash buffer containing 50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Bioengineering 2024Quote: ... The output of differentially expressed proteins was further investigated using Ingenuity Pathway Analysis® (IPA; Qiagen®, Redwood City, USA). Data was also independently analysed using PEAKS studio (Bioinformatics Solutions® Inc. ...
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bioRxiv - Bioengineering 2024Quote: DNeasy Blood and Tissue kit (#69504, QIAGEN) was used to extract DNA from samples according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2024Quote: ... total RNA was extracted using RNeasy kits (Qiagen, Valencia, CA) and quantified by NanoDrop (BioLabNet ...
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Phospho-KNL-1 recognition by a TPR domain targets the BUB-1–BUB-3 complex to C. elegans kinetochoresbioRxiv - Cell Biology 2024Quote: ... Beads were washed twice with re-suspension buffer and once with 20 mM imidazole in resuspension buffer before applying to polypropylene columns (Qiagen). Samples were eluted in 1 mL fractions using 400 mM Imidazole in resuspension buffer ...
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bioRxiv - Cell Biology 2024Quote: ... All qPCR assays were performed on Rotor-Gene Q thermocycler (Qiagen, Toronto, ON, Canada). The relative expression of mRNA and miRNA was normalized to ACTB and snRNA U6 ...
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bioRxiv - Cell Biology 2024Quote: ... The genomic DNA fragment was separated by electrophoresis using a 1% agarose gel and purified using QIAquick Gel Extraction Kit (QIAGEN). The genome sequence of drp-1 was determined by Sanger sequencing using primers listed in Supplementary Table S2.
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bioRxiv - Bioengineering 2024Quote: ... RNA was isolated using the Qiagen RNeasy Kit (Qiagen, Hilden, Germany) following the manufacture’s instruction ...
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bioRxiv - Bioengineering 2024Quote: ... PCR amplification was done with Qiagen’s HotStarTaq Master Mix Kit (Qiagen, Cat. No. 203443), with 20 ng of converted DNA and 0.2 µM for each of primers in 30 µL final volume ...
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bioRxiv - Bioengineering 2024Quote: Total RNA was isolated from untreated or RNP-transfected plerixafor-mobilized HD and SCD HSPCs (n=3 for each group) using the RNeasy Kit (QIAGEN) that includes a DNAse treatment step ...
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bioRxiv - Bioengineering 2024Quote: Genomic DNA was isolated using a DNeasy Blood and Tissue Kit (Qiagen) and PCR amplification was performed with KAPA2G Robust PCR kits (KAPA Biosystems ...
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bioRxiv - Bioengineering 2024Quote: RNA was harvested from cell pellets using the RNeasy Plus Mini Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... the cDNA was synthesized from the extracted total RNA using the miRCURY LNA RT Kit (Qiagen). Briefly ...
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bioRxiv - Biochemistry 2024Quote: ... cells were transfected in 10 cm2 at ∼60% confluency using PolyFect (Qiagen, 301107) and washed with complete culture medium 6 h later ...
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bioRxiv - Biochemistry 2024Quote: Total RNA was isolated from bacterial cells using the RNeasy mini kit (5 mL culture) and RNeasy maxi kit (50 mL culture) (Qiagen). RNA concentrations were measured using a Nanodrop spectrometer (ThermoScientific) ...
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bioRxiv - Biochemistry 2024Quote: ... Tissues were homogenized using Handy Micro Homogenizer (NS310E2, Microtec Co. Ltd., Japan) with 40 µL / mg tissue of RLT buffer (RNeasy Mini Kit, Qiagen, USA), which contained 10 µL / mL of β-mercaptoethanol (β-ME) ...
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bioRxiv - Biochemistry 2024Quote: ... Total RNA was extracted from the supernatant (12,000 x g for 10 min at 4°C) of centrifuged tissue homogenate using an RNeasy Mini Kit (Qiagen, USA). The extracted total RNA was used for single-strand cDNA synthesis ...
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bioRxiv - Biochemistry 2024Quote: Splenocyte derived mRNA was harvested using a QIAmp RNA mini kit (Qiagen, 52304) and converted to cDNA using RNA to cDNA EcoDry Premix (Takara ...
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bioRxiv - Biochemistry 2024Quote: ... using Effectene transfection reagent (Qiagen, Germantown, MD).
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bioRxiv - Biochemistry 2024Quote: ... Supernatants were filtered through a 0.22 µm syringe filter before application to 5 ml of nickel resin (Ni-NTA Superflow, QIAGEN) equilibrated in loading buffer (25 mM HEPES-KOH pH 7.6 ...
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bioRxiv - Biochemistry 2024Quote: Selected cells were harvested for genomic DNA preparation using DNeasy Blood and Tissue kit (Qiagen, Germantown, MD). 2 µg of genomic DNA was digested with one of few combinations of restriction enzymes (Table S5 ...
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bioRxiv - Biochemistry 2024Quote: Total RNA was isolated from cells grown in six-well plates at 70–80% confluency with an RNA extraction kit (Qiagen #74104). cDNA was synthesized using a sensiFAST cDNA synthesis kit (Meridian Bioscience #BIO-65053) ...
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bioRxiv - Biochemistry 2024Quote: ... The lysate was pelleted by centrifugation at 30,000 x g for 30 min and the supernatant was mixed with 5 mL Ni-NTA resin (Qiagen) pre-equilibrated with lysis buffer in a 50 mL falcon tube ...
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bioRxiv - Biochemistry 2024Quote: ... Ingenuity Pathway Analysis (Qiagen, Germany); R studio (Version1.2.5001) ...
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bioRxiv - Bioengineering 2024Quote: ... Genomic DNA and RNA were isolated from homogenized tissues using the DNEasy Blood and Tissue Kit (Qiagen, LLC) and RNeasy Kit (Qiagen ...
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bioRxiv - Bioengineering 2024Quote: ... The sized samples were purified with MinElute columns (Qiagen, Hilden, Germany) and started in library prep with 10ng each for the ‘long’ samples and 8ng each for the ‘short’ ...
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bioRxiv - Bioengineering 2024Quote: ... The tissue was pulverized (6775 Freezer/Mill SPEX) and the total RNA was extracted with RNeasy minicolumns (RNeasy Fibrous Tissue Kit, Qiagen). RNA concentration was quantified using a Qubit 4 Fluorometer (Thermo Fisher) ...
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bioRxiv - Biochemistry 2024Quote: ... Ingenuity Pathway Analysis (Qiagen, Germany); R studio (Version1.2.5001) ...
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bioRxiv - Biochemistry 2024Quote: ... PCR arrays were performed using RT2 Profiler™ PCR Array Mouse Cytokines & Chemokines (PAMM-150Z, Qiagen). Calculations were performed by a comparative method (2−ΔΔCt ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were detached by trypsin treatment and RNAs were extracted using miRNeasy Mini Kit (Qiagen). RNA concentration was determined by NanoDrop One C (ThermoFisher Scientific) ...