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Citations for Qiagen :
151 - 200 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... and Total RNA was isolated using miRNeasy mini kit (QIAGEN, Cat# 217004) according to the manufacturer’s protocol and as previously described22,65 ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were lysed with QIAzol lysis reagent (QIAGEN, Cat# 79306), and Total RNA was isolated using miRNeasy mini kit (QIAGEN ...
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bioRxiv - Microbiology 2024Quote: ... One hundred microliters (uL) PBS was added to NW pellets followed by 280uL AVL lysis buffer (Qiagen, Germantown, MD, USA). Two to three mL blood was collected from the cranial vena cava in an SST tube for serum separation ...
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bioRxiv - Microbiology 2024Quote: ... Carrier RNA was added to each sample and RNA was extracted using EZ1 DSP kit on a Qiagen EZ1 Advanced XL extractor according to the manufacturer’s instructions (Qiagen, Germantown, MD, USA). RNA was eluted in 120 uL RNase-free water and stored at -80°C until evaluated for gene expression by qRT-PCR ...
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bioRxiv - Microbiology 2024Quote: DNA was extracted from cryopreserved stool and colon biopsies using the PowerFecal DNA Isolation Kit and the company’s recommended protocol (Qiagen, Valencia, CA). Sequencing libraries were prepared as described by the Earth Microbiome Project [53] and sequenced on an Illumina MiSeq Sequencer (Illumina ...
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bioRxiv - Cell Biology 2024Quote: ... DNA fragments ranging from 350 to 700 bp were excised from the gel and purified using a QIAGEN gel extraction kit (QIAGEN). PCR and library preparation were conducted as previously described25 ...
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bioRxiv - Cell Biology 2024Quote: ... or QIAamp DNA Blood Midi Kit (QIAGEN). The purified DNA was digested overnight with SbfI-HF (New England Biolabs ...
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bioRxiv - Microbiology 2024Quote: ... To optimize the DNA extraction the supernatant was taken off and digested with RNAse A (Qiagen). The DNA was then automatically purified with the QIAamp 96 QIAcube HT kit (Qiagen #51331 ...
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bioRxiv - Microbiology 2024Quote: ... The DNA was then automatically purified with the QIAamp 96 QIAcube HT kit (Qiagen #51331) with additional proteinase K on a QIAcube HT following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The pellet from the first centrifugation was resuspended in 200 µL of PBS and DNA was isolated using the cultured cells protocol of the Dneasy Blood and tissue Kit (Qiagen). The second pellet ...
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bioRxiv - Cell Biology 2024Quote: RNA was isolated from RPE-1 cells using the RNeasy mini kit (Qiagen, CA) and cDNA was generated using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Microbiology 2024Quote: ... and extracted twice in stool lysis buffer ASL (Qiagen, Hilden Germany). Bead beating at a speed of 5 m/s for 60 seconds (x2 ...
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bioRxiv - Microbiology 2024Quote: ... and purified using QIAamp DNA Mini Kit (Qiagen). The purified DNA was tested for purity with a nanophotometer (Implen NP80 ...
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bioRxiv - Microbiology 2024Quote: ... Swabs were DNA extracted using a DNeasy blood and tissue kit (Qiagen). Living frogs were weighed at day 30 and day 60 to estimate health and body condition.
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bioRxiv - Microbiology 2024Quote: ... The QIAseq miRNA Library Kit (Qiagen) was used to prepare cDNA libraries ...
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bioRxiv - Cell Biology 2024Quote: ... Gel slices corresponding to fragments approximately 147 - 160 bp in size were then extracted using the MinElute Gel Extraction Kit (Qiagen, #28604). Libraries were quantified with Qubit dsDNA HS Kit (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was then cleaned up using a MinElute Reaction CleanUp Kit (Qiagen). To reduce adaptor dimers ...
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bioRxiv - Microbiology 2024Quote: ... samples were resuspended in 100 μl TE buffer pH 8 and RNA extraction performed according to the RNeasy Mini Kit (Qiagen) protocol with few modifications ...
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bioRxiv - Microbiology 2024Quote: DNA was isolated by mechanical disruption using glass beads in a TissueLyser II bead mill (Qiagen, Hilden, Germany) (66 ...
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bioRxiv - Microbiology 2024Quote: ... total RNA of cells was extracted using an RNeasy Mini Kit (Qiagen, USA). Samples were then incubated with RNase-free DNase I (Promega ...
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bioRxiv - Microbiology 2024Quote: ... the cell pellet was resuspended in 600 µL ATL buffer (Qiagen #939011) and transferred to a ZR BashingBead Lysis Tube (Zymo Research #S6012-50) ...
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bioRxiv - Microbiology 2024Quote: ... Total cellular DNA was extracted from approximately 0.5 to 5 million cells using a QIAamp DNA Blood Minikit (Qiagen). PCR assays were performed with 200 ng samples of DNA ...
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bioRxiv - Microbiology 2024Quote: ... The DNA was then recovered using a MinElute gel extraction kit (Qiagen) using 4 PE buffer washes.
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bioRxiv - Microbiology 2024Quote: ... Genomic DNA was extracted from 13 ml of the culture using a DNeasy Blood & Tissue kit (Qiagen) according to the manufacturer’s instructions for Gram-negative bacteria ...
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bioRxiv - Microbiology 2024Quote: ... Plasmid DNA was isolated from Escherichia coli using a Spin MiniPrep kit (Qiagen) and transferred to X ...
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bioRxiv - Microbiology 2024Quote: Plasmid DNA was amplified using REPLI-g® Midi Kit (QIAGEN), according to the manufactureŕs instructions ...
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bioRxiv - Microbiology 2024Quote: ... thermophilus HB27 genomic DNA extracted using the DNeasy UltraClean MicrobialKit (QIAGEN). Amplified genes were digested and inserted into the pET28b(+ ...
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bioRxiv - Microbiology 2024Quote: ... DNase treatment was performed using an RNase-Free DNase kit (Qiagen, Maryland, USA) according to the manufacturer’s protocol to remove any contaminant genomic DNA ...
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bioRxiv - Microbiology 2024Quote: ... We used the DNeasy power soil kit (Qiagen, Maryland, USA) to extract fecal samples and intestinal contents of the mice ...
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bioRxiv - Microbiology 2024Quote: Genomic DNA was extracted from pure bacterial cultures using a DNeasy blood and tissue kit (Qiagen, Maryland, USA). We used the DNeasy power soil kit (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... The combined tissues were submerged in 600μl of lysis buffer containing 30 μl of foaming reagent (Reagent DX, Qiagen) and 60μl of β-mercaptoethanol (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2024Quote: ... Tissue samples were homogenized with a TissueRuptor (Qiagen) for up to 1 minute at 5,000 rpm ...
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bioRxiv - Cell Biology 2024Quote: ... RNA was isolated using the RNeasy Mini Kit (QIAGEN, #74106) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and are carried out underneath the hydrophobic overlay Vapor-lock (QIAGEN, #981611). Instead of cleaning up the cDNA ...
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bioRxiv - Cell Biology 2024Quote: For Smart-seq3 LV library preparation each well was dispensed with 3µL of Vapor-lock (Qiagen) prior addition of lysis buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... Live single cells were sorted into 96 wells plates containing 10µl of TCL lysis buffer (Qiagen 1031576), supplemented with 1% β-mercaptoethanol.
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bioRxiv - Molecular Biology 2024Quote: ... Cells were harvested and total RNA isolated using an RNA isolation kit (RNeasy Mini kit, Qiagen, 74106). 8 µg of total RNA were treated with DNaseI and purified according to the manufacturer’s protocol (TURBO DNA-free kit ...
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bioRxiv - Cell Biology 2024Quote: RNeasy Micro Kit (Qiagen; cat. #74004) was used to extract whole cell RNA ...
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bioRxiv - Cancer Biology 2024Quote: ... Purified plasmid DNA was generated from overnight LB cultures with the QIAprep Spin Miniprep Kit (Qiagen, #27106X4) and quantified using the NanoDrop ND-1000 spectrophotometer (ThermoFisher ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was isolated from Ogt iKO and Ctrl mESC samples using RNeasy plus mini or micro kit (Qiagen). RNA-sequencing libraries were prepared using Truseq stranded mRNA kit (Illumina ...
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bioRxiv - Molecular Biology 2024Quote: ... and 10 μl of Quantifast SYBR Green PCR Master Mix (QIAGEN) or PowerUp™ SYBR™ Green Master Mix (ThermoFisher) ...
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bioRxiv - Molecular Biology 2024Quote: ... Data were further analyzed with the use of QIAGEN IPA (QIAGEN Inc. ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA from fibroblasts subjected to different stiffness conditions and drug treatments was isolated and purified using the RNeasy Plus Mini Kit (QIAGEN). cDNA was synthesized from 1 µg RNA with the iScript cDNA Synthesis Kit (Bio-Rad) ...
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bioRxiv - Cancer Biology 2024Quote: ... Colonies were scraped off the plates and plasmids were extracted with a plasmid maxiprep kit (Qiagen).
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bioRxiv - Cancer Biology 2024Quote: ... Genomic DNA (gDNA) was extracted with a DNeasy Blood and Tissue kit (Qiagen). A two-step amplification protocol adapted from Chan et al ...
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bioRxiv - Cancer Biology 2024Quote: ... Total RNA from 7 of these patients was extracted from CD138+ cells using the miRNeasy Mini Kit (Qiagen GmbH, Germany) according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2024Quote: ... genotyping PCR products were purified using MinElute PCR Purification Kit (28006X4, Qiagen), DNA concentration was measured by spectrophotometry ...
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bioRxiv - Cancer Biology 2024Quote: ... and DNA was isolated using the MinElute PCR Purification Kit (28006X4, Qiagen).
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bioRxiv - Cancer Biology 2024Quote: Genomic DNA was isolated using DNeasy Blood & Tissue Kit (Qiagen). Libraries for Whole Exome Sequencing (WES ...
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bioRxiv - Cancer Biology 2024Quote: RNeasy mini kit (Qiagen) was used to extract RNA from cells ...