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Citations for Qiagen :
501 - 550 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... and purified using QIAquick PCR Purification Kit (QIAGEN). The quality and concentration of the linearized plasmid were confirmed via gel-electrophoresis and Nanodrop Spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2024Quote: Extraction of mRNA was performed using the RNA extraction kit (Qiagen, Venlo, Netherlands, # 74106) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... Pre-equilibrated nickel-NTA resin (Qiagen, 50 mg/mL binding capacity) was added to the clarified lysate and the lysate-resin slurry stirred in a beaker with a stir bar for 1 hour at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... were used with HiPerFect transfection Reagent (QIAGEN). Cells treated with siRNA were incubated for 48hr prior to live imaging or sample collection for quantitative analysis ...
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bioRxiv - Cell Biology 2024Quote: ... Effectene (Qiagen) was used to transfect cells according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... The plasmid was extracted with a plasmid extraction kit from QIAGEN. Afterwards ...
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bioRxiv - Cell Biology 2024Quote: ... FlexiTube GeneSolution for uchl5 and usp14 (QIAGEN) and AllStars Negative Control siRNA (QIAGEN ...
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bioRxiv - Cell Biology 2024Quote: ... and AllStars Negative Control siRNA (QIAGEN) were used with HiPerFect transfection Reagent (QIAGEN) ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were homogenized with a 5-mm steel bead in a TissueLyser bead mill (Qiagen; operated at 50 Hz for 30 seconds). The homogenized samples were incubated at room temperature for 10 minutes for complete cell lysis and then centrifuged at maximum speed for 5 minutes to pellet insoluble material ...
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bioRxiv - Cell Biology 2024Quote: ... using a TissueLyser bead mill (Qiagen). Samples were denatured at 95 °C for 5 minutes ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was isolated from hAC using the RNeasy Mini Kit (Qiagen) and afterwards reverse transcribed to cDNA by means of the Superscript II Reverse Transcriptase (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was prepared from the hCiPSC using the RNeasy Plus Mini Kit (Qiagen). Reverse transcription was performed using the High-Capacity cDNA Reverse Transcription Kit (Life Technologies) ...
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bioRxiv - Cell Biology 2024Quote: ... RNA was reverse transcribed with the Omniscript RT Kit (Qiagen), according to the manufacturer`s protocol.
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bioRxiv - Cell Biology 2024Quote: ... tissue was snap frozen in liquid nitrogen and pulverized with a microdismembrator S (B. Braun Biotech, Melsungen, Germany) before total RNA isolation by means of the Lipid Tissue Mini Kit (Qiagen, Hilden, Germany). Isolated cells were lysed in RLT buffer ...
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bioRxiv - Biophysics 2024Quote: ... For cRNA purification the RNeasy Mini Kit (QIAGEN) was used ...
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bioRxiv - Cancer Biology 2024Quote: ... Genomic DNA was isolated using the DNeasy kit (Qiagen, Venlo, Netherlands) following the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2024Quote: Genomic DNA was extracted from the frozen tissues with PureGene Core Kit A (Qiagen). Barcode sequences were PCR amplified for NGS by introducing Illumina adaptors and 10-bp-long index sequences ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was synthesized from 500 ng of RNA using QuantiTect Reverse Transcription Kit (Qiagen). Quantitative real-time PCR was performed using Select Master Mix (Thermo Fisher ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted from CD33+ ITGA7-sorted primary AML cells (> 4x105 cells/sample) using the RNeasy Mini kit (Qiagen, 74106) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted and purified from the cardiac tissues using the RNeasy mini kit (Qiagen, Germantown, MD). cDNA was synthesized from 500 ng of RNA using QuantiTect Reverse Transcription Kit (Qiagen) ...
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bioRxiv - Cancer Biology 2024Quote: DNA was extracted from each sample using DNA Mini Kit (Qiagen) with the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA from tumor and normal cells with the indicated treatments was extracted using the miRNAeasy Plus kit (Qiagen) and used for the analysis of gene expression using RT-qPCR (Four replicates ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted from samples (miRNeasy mini kit; Qiagen), quantified using Nanodrop 8000 ...
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bioRxiv - Cancer Biology 2024Quote: ... Pathway and biological processes analysis of all DEGs was performed using Ingenuity Pathway Analysis (IPA, Qiagen, RRID:SCR_008653). DEGs were filtered to select the targets altered in both cell lines ...
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bioRxiv - Cancer Biology 2024Quote: ... and cotransfected with SV40 Renilla Luciferase and ARR3-Luciferase constructs using Effectene Transfection Reagent (Qiagen). Luciferase activity was assayed 48 hr after transfection using Dual-Luciferase Reporter Assay System (Promega) ...
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bioRxiv - Cancer Biology 2024Quote: ... a pool of 15 million cells from each condition were harvested for genomic DNA (gDNA) isolation using the DNeasy blood and tissue kit (Qiagen) according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2024Quote: ... Real-time PCR was performed using 2X SYBR green quantfast PCR Mix (Qiagen, 1044154) and gene-specific primers as follows ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was isolated from cells using the miRNeasy kit (Qiagen), and cDNA was synthesized from 1000 ng of total RNA using Maxima First Strand cDNA Synthesis Kit for RT-qPCR (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... Maxipreps (Qiagen) and minipreps (Promega ...
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bioRxiv - Cell Biology 2024Quote: The DNeasy Blood & Tissue Kit (QIAGEN) was used according to the manufacturer’s instructions to extract mtDNA from mouse liver for injection into wound beds ...
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bioRxiv - Cell Biology 2024Quote: ... RNA was then extracted using the RNeasy (Qiagen, Germantown, MD, USA) kit and cDNA was synthesized using the SuperScript™ III Reverse Transcriptase kit (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... the DNeasy Blood & Tissue Kit (QIAGEN) was utilized following the manufacturer’s instructions to isolate the cell-free mtDNA in plasma and cell supernatant ...
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bioRxiv - Cell Biology 2024Quote: ... membranes were washed twice with PBS and cells were lysed on the membrane using RLT plus buffer and processed following the manufacturer’s protocol with the addition of an extra elution step to maximize RNA concentration (RNeasy Plus Mini Kit; Qiagen). RNA was stored at -80 °C prior to use.
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bioRxiv - Cancer Biology 2024Quote: ... and AR DBD domain DNA fragment were cut by NheI (N-terminal) and XhoI (C-terminal) and purified with the QIAquick Gel Extraction Kit (Qiagen; cat. 28704). After ligation with T4 DNA Ligase (New England ...
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bioRxiv - Cancer Biology 2024Quote: ... purified them with the Qiagen PCR purification kit (Qiagen; cat. 28004) and verified all products by gel separation and sanger sequencing to ensure they were unmutated.
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bioRxiv - Cancer Biology 2024Quote: ... and DNaseI treated by RNeasy Mini Kit (Qiagen; cat. 74104). Nanodrop quantified RNA was checked by Bioanalyzer RNA 6000 Nano Kit (Agilent Technologies) ...
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bioRxiv - Cancer Biology 2024Quote: ... then purified with the Qiagen PCR purification kit (Qiagen; cat. 28004).
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bioRxiv - Cancer Biology 2024Quote: Genomic DNA was prepared from flushed marrow of cell line-injected tibiae using DNeasy Blood & Tissue Kit (#69504, Qiagen, Hilden, Germany,). A non-injected mouse bone marrow was used as a negative control ...
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bioRxiv - Cancer Biology 2024Quote: ... Genomic DNA from tumor tissue and blood was isolated using the DNeasy Blood & Tissue Kit (69504, Qiagen, Netherlands), following the manufacturer’s instructions ...
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Increased dosage of wild-type KRAS protein drives KRAS-mutant lung tumorigenesis and drug resistancebioRxiv - Cancer Biology 2024Quote: ... Amplified sequences were purified with a QiaQuick PCR purification kit (Qiagen, 28104). Further library preparation and Illumina sequencing (NovaSeq PE150 ...
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bioRxiv - Cancer Biology 2024Quote: RNA was extracted using an Rneasy Kit (Qiagen), and reverse-transcribed with the Lunascript Reverse Transcription kit (New England Biolab ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted using RNeasy micro-kit (Qiagen) following manufacturer’s procedures ...
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bioRxiv - Cancer Biology 2024Quote: RNA was isolated from 10-μm FFPE samples from resected canine primary melanoma tumor or metastatic tumor lesions using an RNEasy FFPE Kit and deparaffinization solution (Qiagen). Isolated RNA was examined by Bioanalyzer (Agilent ...
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bioRxiv - Cancer Biology 2024Quote: ... and DNA was extracted using AllPrep DNA/RNA Mini Kit (Qiagen). 50ng of gDNA from blood (germline ...
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bioRxiv - Cancer Biology 2024Quote: RNA was extracted from the cells dissociated with C&H or dispase or from fresh frozen tissues using RNeasy plus mini kit (#74134, Qiagen). 500 ng of RNA from each sample was used for cDNA synthesis using High-capacity RNA-to-cDNA kit (#4387406 ...
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bioRxiv - Cancer Biology 2024Quote: ... respectively (both from Qiagen, Germantown, MD) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted from cells with RNeasy Plus Mini Kit (Qiagen, 74136) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... cultures and verified with DNA digestion and gel electrophoresis before clonal expansion and purification with QIAGEN Plasmid Maxi Kit was performed (12163; Qiagen, Redwood City, CA, USA). Lentivirus particles were produced in HEK293T cells after co-transfection of lentivirus plasmid vectors with packaging plasmids (VSVG and lenti gag/pol ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted from the organoids using the RNeasy mini kit (QIAGEN, Venlo, Netherlands). The concentration of the extracted total RNA was determined using the Nanodrop 2000 system (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... and RNeasy Kit (Qiagen #74104), as directed ...