Labshake search
Citations for Qiagen :
1401 - 1450 of 1924 citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... The in-house plasmid preparation was carried out following the manufacturer’s protocol (Qiagen Midi-kits) and eluted in deionized water ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... plasmids were extracted from the competent cells with QIAprep Spin Miniprep Kit (Qiagen, Venlo, Netherlands) and sent to the Australian Genome Research Facility for Sanger sequencing to verify the correct insertion of the guide sequences ...
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bioRxiv - Cell Biology 2024Quote: ... and transfer plasmids in a 45:5:50 ratio using PolyFect transfection reagent (Qiagen #301107) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA was prepared from a bacterial extract using the EndoFree Plasmid Maxi Kit (#12362, Qiagen) and digested with MluI to release the plasmid backbone ...
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bioRxiv - Cancer Biology 2024Quote: ... we picked white colonies for amplification and plasmid isolation by QIAprep Spin miniprep kit (QIAGEN). Lastly ...
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bioRxiv - Molecular Biology 2024Quote: pLicC-MBP-ERR1712142 plasmid was extracted from bacterial lysate with Maxiprep (Qiagen Cat No. 12662) after overnight incubation in BL21 strain BL21 (λDE3 ...
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bioRxiv - Immunology 2024Quote: ... Plasmid DNA was purified with the help of the QIAprep Spin Miniprep Kit (#27104, QIAGEN). Successful mutagenesis was verified by Sanger sequencing (LGC Genomics) ...
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bioRxiv - Molecular Biology 2024Quote: ... positive transformants were verified by sequencing and plasmids prepared using the Qiagen miniprep kit (Qiagen). Resulting CRISPR/Cas9 lentiviral constructs were transiently transfected along with packaging plasmids pCMV-dR8.2dvpr and pCMV-VSV-G (Addgene ...
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bioRxiv - Bioengineering 2021Quote: Genomic DNA from human primary CD4+/CD8+ T cells was isolated using the Gentra Puregene Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... frozen fragments of human cortex were homogenized with a motorized hand pestle in RLT lysis buffer (Qiagen) and qPCR was performed as described in qPCR section.
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bioRxiv - Microbiology 2021Quote: Genomic DNA (gDNA) from human blood cells was isolated using the QIAamp Blood kit (QIAGEN, Hilden, Germany). To isolate gDNA from different mouse tissues we made use of the blackPREP Rodent Tail DNA Kit (Analytik Jena ...
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bioRxiv - Cancer Biology 2020Quote: Total genomic DNAs (containing human and viral genomes) were isolated using AllPrep DNA/RNA Mini Kit (Qiagen) from the NPC biopsy ...
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bioRxiv - Cancer Biology 2020Quote: DNA from human and murine PC cells were isolated using the DNeasy blood and tissue kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... The RT2 Profiler “Inflammatory cytokines and receptors” and “Human innate and adaptive response” arrays PCR Arrays (Qiagen) were performed according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... Genomic human DNA was isolated from whole blood using QIAamp DNA Blood Mini Kit from Qiagen (51106) and phosphodiester DNA from TIB Molbiol ...
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bioRxiv - Paleontology 2019Quote: Human 2 and 3: DNA was extracted from bones using QIAamp® DNA Investigator kit (56504, Qiagen). Bones were thoroughly washed (X5 ...
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bioRxiv - Microbiology 2020Quote: ... Two mouse and two human stool samples were homogenized using a PowerLyzer 24 Homogenizer (110/220V; Qiagen). DNA was extracted using four different bead beating times ...
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bioRxiv - Genetics 2019Quote: RNA was extracted from PCYT1A-wild-type human fibroblasts using the RNeasy Mini Kit (Qiagen, Cat#74104), reverse-transcribed according to the manufacturer’s protocol (SuperScriptIII One-Step RT-PCR system with Platinum Taq DNA Polymerase;Invitrogen ...
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bioRxiv - Genetics 2024Quote: The RNA quality of human kidney FFPE sample was checked by RNeasy FFPE kit (Qiagen-Cat #73504) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA was extracted from human tissue and cells using the miRNeasy Mini kit (Qiagen, Hilden, Germany) or TRIzol reagent (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2023Quote: RNA from 1205Lu cells and immortalized human melanocytes were isolated and purified using RNeasy Micro Kit (Qiagen) according to manufacturer’s instruction and concentration was quantified using a NanoDrop Spectrophotometer (ThermoScientific) ...
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bioRxiv - Genomics 2024Quote: ... the effect of human rRNA removal was also assessed using a QIAseq FastSelect rRNA removal kit (Qiagen). The rRNA removal reaction was performed after RNA thermal denaturation at 95℃ 5 min according to the instructions.
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bioRxiv - Cell Biology 2022Quote: ... Human ATGL-targeting and cPLA2α-targeting siRNAs and the AllStars Negative Control siRNA were from Qiagen (Germany). T863 (DGAT1 inhibitor) ...
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bioRxiv - Developmental Biology 2022Quote: ... Reads from *.fastq files were mapped to the rat reference genome (Rattus norvegicus reference genome Rnor_6.0) or the human reference genome (Homo sapiens reference genome GRCh37) using CLC Genomics Workbench 20.0.4 (Qiagen). Only reads with <2 mismatches and minimum length and a similarity fraction of 0.8 were mapped to the reference genome ...
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bioRxiv - Cell Biology 2023Quote: Total RNA of human macrophages was isolated according to manufacturer’s instructions using the RNeasy extraction kit (Qiagen). mRNA was extracted using the Next Poly(A ...
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bioRxiv - Cell Biology 2023Quote: Uninfected or infected primary human macrophages were subjected to RNA extraction using the RNeasy extraction kit (Qiagen). Two µg of RNA each was reverse transcribed with the iScript cDNA Synthesis Kit (Bio-Rad) ...
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bioRxiv - Immunology 2022Quote: Genomic DNA was purified from human peripheral blood mononuclear cells (PBMCs) using QIAamp kits (Qiagen, Hilden, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... and the pellet was lysed using RLT buffer (mouse cells) or QIAZol (human cells) (Qiagen, Cat. 79306). RNA was extracted using the Qiagen RNeasy micro kit.
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bioRxiv - Immunology 2024Quote: Microbial DNA was obtained from fecal samples (humans or mice) using the DNeasy PowerSoil Pro kit (Qiagen) according to manufacturer’s instructions with an added cell lysis step at TissueLyser II bead beater (Qiagen) ...
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bioRxiv - Cell Biology 2020Quote: ... plasmids encoding putative interactors were transfected in triplicate using the Effectene transfection reagent (Qiagen, Hilden, Germany) and a final concentration of 400µM BSA-bound oleic acid were added ...
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bioRxiv - Developmental Biology 2021Quote: ... Plasmid DNA was then extracted from chosen colonies using a Qiaprep spin Mini-prep kit (Qiagen) and sequenced (Applied Biosystems’ 3730 DNA Analyser ...
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bioRxiv - Microbiology 2021Quote: ... The plasmid containing the cloned chitinase gene was isolated using the Qiaprep spin miniprep kit (Qiagen). Restriction digestion of the plasmid was done with XhoI and XmaI ...
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bioRxiv - Genomics 2020Quote: ... The plasmid DNAs were prepared using QIAprep Spin Miniprep Kit (catalog No. 27104, QIAGEN, Beijing, China). The appropriate restriction enzymes were then used to determine the presence or absence of methylated motifs in recombinant plasmid ...
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bioRxiv - Plant Biology 2019Quote: ... pDONR207-sgGBSS1 and pDONR207-sgGBSS2 were purified using the QIAGEN Plasmid Plus Midi Kit (QIAGEN, Germany), followed by a sodium acetate precipitation.
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bioRxiv - Bioengineering 2020Quote: Genomic and plasmid DNA were extracted from transformants using a DNeasy Blood & Tissue kit (Qiagen, Germany) and Exprep plasmid SV kit ...
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bioRxiv - Cancer Biology 2020Quote: Plasmid was extracted from 1.5 ml of bacterial culture with the QIAamp DNA Mini Kit(Qiagen) and purified for sequencing validation (1st BASE) ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were co-transfected with the C6orf203-TAP construct and with plasmid pOG44 using Superfect (Qiagen). After 48h ...
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bioRxiv - Bioengineering 2021Quote: ... and only the plasmid DNA was separated through the column (Qiagen, QIAquick® PCR purification Kit).
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bioRxiv - Immunology 2021Quote: ... Plasmid DNA was prepared from overnight cultures using the QIAprep Spin Miniprep kit (QIAGEN, Hilden, Germany) according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... Isolation and purification of the pDNA was done using a Plasmid Plus maxi kit (QIAGEN, UK) and a NanoDrop One Microvolume UV-Vis Spectrophotometer (ThermoFisher ...
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bioRxiv - Cell Biology 2021Quote: ... Purified PCR products amplified from plasmids isolated from a single bacterial colony (QIAprep Spin MiniprepKIT, Qiagen) were labeled by random priming with digoxigenin-11-dUTP (Roche ...
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bioRxiv - Microbiology 2022Quote: ... Agarose gel purification and DNA plasmid extractions were performed using a QIAquick Gel extraction kit (QIAGEN). Restriction enzyme digests were performed according to manufacturer recommendations (Biolabs) ...
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bioRxiv - Biochemistry 2022Quote: ... Plasmid or oligo-derived transcription templates were cleaned up using a MinElute PCR Purification Kit (Qiagen). SpCas9 gRNAs were transcribed at 37 °C for 16 hours using the T7 RiboMAX Express Large Scale RNA Production Kit (Promega) ...
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bioRxiv - Developmental Biology 2022Quote: ... Mary Goldring) and 10 ng of pRL Renilla luciferase plasmid using the Effectene transfection reagent (Qiagen). Cells were transfected for 8 hrs and then cultured for 24 hrs ...
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bioRxiv - Genomics 2022Quote: ... and plasmid DNA of positive clones was obtained using QIAprep Spin Miniprep Kit (Qiagen, Cat#: 27106). Clones were verified as mutation-free by capillary sequencing ...
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bioRxiv - Developmental Biology 2019Quote: ... 3 million cells were seeded and transfected with 1μg pMK33-based plasmid DNA using Effectene (Qiagen). After 48 hours ...
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bioRxiv - Microbiology 2019Quote: ... The plasmids were extracted from these positive bacterial colonies (QIAprep Spin Miniprep Kit, Qiagen, Hilden, Germany) and submitted for bidirectional ...
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bioRxiv - Molecular Biology 2019Quote: ... pGEMT-UnFosb or pGEMT-Fosb) and reference plasmid (pGEMT-Gapdh) using Rotor-gene Q software (Qiagen). Primer sequences are found in Supplementary Table 3.
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bioRxiv - Cancer Biology 2020Quote: ... All plasmids used for in vivo experiments were purified using the Endotoxin-free Maxiprep kit (Qiagen). [U-13C]-glucose ...
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bioRxiv - Developmental Biology 2019Quote: ... All DNA plasmids used in electroporation experiments were purified using Midiprep DNA isolation kits (Qiagen, 12143) and resuspended in Tris-EDTA (TE ...