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Citations for Qiagen :
1451 - 1500 of 1799 citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The plasmids were recovered from overnight cultures of positive clones with a QIAquick Spin miniprep kit (Qiagen), and the sequence of the flanking regions was confirmed by in-house sequencing with their respective construction primers and sequencing primers listed in Table S2 ...
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bioRxiv - Microbiology 2023Quote: ... The plasmid was recovered from overnight cultures of positive clones with a QIAquick Spin miniprep kit (Qiagen) and the sequence of the flanking regions was confirmed by in-house sequencing with their respective construction primers and sequencing primers listed in Table S2 ...
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bioRxiv - Microbiology 2023Quote: ... The plasmid was recovered from overnight cultures of positive clones with a QIAquick Spin miniprep kit (Qiagen), and the sequence of the flanking regions was confirmed by in-house sequencing with their respective construction primers and sequencing primers listed in Table S2.
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bioRxiv - Biochemistry 2023Quote: ... Plasmid DNA was extracted and purified from 5 ml or 250 ml bacterial culture using Miniprep (Qiagen) or Maxiprep (Thermo-Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... The plasmid sub-libraries were purified from the bacteria using the QIAamp DNA Mini Preparation Kit (Qiagen). The DNA concentration was measured using the Quant-iT dsDNA (HS ...
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bioRxiv - Bioengineering 2023Quote: ... coli and purified for cell culture application through commercially available kits (Qiagen, Maxi Plasmid Kit, Cat# 12162).
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bioRxiv - Systems Biology 2023Quote: The amplified vectors were extracted from the inoculated culture with Plasmid Plus 96-well Miniprep kit (Qiagen). The concentration was measured with a Nanophotometer and diluted to 100 ng/µl ...
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bioRxiv - Systems Biology 2023Quote: The amplified vectors were extracted from the inoculated culture using Plasmid Plus 96-well Miniprep kit (Qiagen). The concentration of each vector was measured with a Nanophotometer and diluted to 100 ng/µl ...
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bioRxiv - Bioengineering 2023Quote: ... phagemid DNA was extracted from bacterial culture using the QIAprep spin mini prep plasmid extraction kit (Qiagen) with OD260 value was measured by NanoDropTM2000 (ThermoFisher ...
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bioRxiv - Genetics 2023Quote: ... Plasmid DNA was isolated from the pooled transformant cells using a High-Speed Maxi Prep kit (Qiagen).
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bioRxiv - Biochemistry 2023Quote: DNA plasmids were isolated from the overnight cultures using the QIAprep Spin Miniprep Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... Cells were harvested by centrifugation and plasmid DNA was isolated using the QIAGEN Midiprep Plus kit (QIAGEN). Plasmid pools ...
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bioRxiv - Developmental Biology 2023Quote: ... The plasmid was linearized by ScaI restriction digest and purified using the QIAquick PCR purification kit (Qiagen). Purified linear plasmid was transfected using Lipofectamine 3000 (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... All colonies were collected by scraping and the DNA was extracted using the Plasmid maxiprep (Qiagen #12165). For a small proof-of-principle library containing 7 different targets ...
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bioRxiv - Cancer Biology 2023Quote: ... Took off the tube and used 30 µL 0.1X EB buffer (From QIAGEN Plasmid Plus Midi Kit) to resuspend the beads and incubated for more than 3 minutes ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We then performed plasmid extractions from the overnight cell cultures using the QIAprep Spin Miniprep Kit (QIAGEN) for plasmid purification ...
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bioRxiv - Cancer Biology 2023Quote: ... Plasmid DNA from single colonies was extracted with the QIAprep Spin Miniprep Kit and Maxi Kits (Qiagen) and confirmed by Sanger sequencing (Genewiz ...
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bioRxiv - Microbiology 2023Quote: Protein complexes were produced from the relevant pQE70 plasmid (see Table S2) in strain M15 pREP4 (Qiagen) as follows ...
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bioRxiv - Systems Biology 2024Quote: ... and a frozen aliquot of each was later collected and plasmids were extracted via miniprep kit (Qiagen).
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bioRxiv - Neuroscience 2024Quote: ... Resulting plasmids were verified with diagnostic restriction digest and Sanger sequencing prior to being maxi-prepped (Qiagen) for subsequent use in excitotoxicity assays.
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bioRxiv - Genomics 2024Quote: ... Plasmid DNA was isolated from individual clones using the QIAprep Spin miniprep kit (Qiagen, Cat. No. 27106), and the cloned plasmids were subjected to sequencing using T7 forward primer and SP6 reverse primer designed for the vector backbone ...
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bioRxiv - Cancer Biology 2024Quote: ... Purified plasmid DNA was generated from overnight LB cultures with the QIAprep Spin Miniprep Kit (Qiagen, #27106X4) and quantified using the NanoDrop ND-1000 spectrophotometer (ThermoFisher ...
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bioRxiv - Cell Biology 2024Quote: ... The linearised plasmid was run on agarose gel and purified with QIAquick Gel Extraction Kit (Qiagen; #28704). sgRNA oligonucleotides (TAG Copenhagen AS ...
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bioRxiv - Developmental Biology 2020Quote: ... we differentiated human stem cells for 3 days in mTeSR + 0.5 µM A8301 and extracted RNA with RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... were used for preamplification and qPCR arrays (Rat Antibacterial Response, PARN-148ZE, and Human Antibacterial Response, PAHS-148ZC; Qiagen) were used for the expression analysis of 84 genes involved in innate antibacterial responses in human and rat respectively ...
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bioRxiv - Molecular Biology 2019Quote: ... mRNA expression levels were normalized to the housekeeping human TATA-binding protein (TBP) mRNA (RT2 qPCR Primer Assays, Qiagen) in the GAL4 assay and to the human housekeeping hypoxanthine guanine phosphoribosyltransferase (HPRT ...
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bioRxiv - Cancer Biology 2019Quote: ... and human BON1 cell line [8] were isolated with RNeasy Plus Universal Kits (Qiagen Cat no. 73404, Germantown, MD) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... and transfected with 1 μg of a pcDNA3.1 vector containing human αSyn cDNA (provided by T. Baron, Anses, Lyon, France) using Effecten transfection reagent (Qiagen). Positive clones were selected using geneticin and cultured in Dulbecco’s modified Eagle’s medium (DMEM/F-12 ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA expression of 84 apoptotic genes was analyzed using the RT2 Profilμer PCR Human Apoptosis Array (Qiagen, PAHS-012Z). Arrays were prepared according to the manufacturer’s protocols applied to the prepared cDNA samples ...
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bioRxiv - Cancer Biology 2019Quote: ... and was run on miScript miRNA PCR Array Human Ovarian Cancer plates (Qiagen, MIHS-110ZE-4, 384 well plate). PCR plates were read the ABI PRISM 7900HT Sequence Detection System (Applied Biosystems) ...
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bioRxiv - Microbiology 2019Quote: ... miScript miRNA PCR array of Human Neurological Development and Disease was performed using miScript SYBR green PCR kit (Qiagen). The PCR array contains 84 miRNAs which are differentially expressed during neuronal development and are responsible for progression of neurological diseases.
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bioRxiv - Bioengineering 2020Quote: ... each SeraCare panel member was diluted 1:10 in human plasma to a final volume of 100μL and processed by QIAGEN QIAamp viral mini kit ...
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bioRxiv - Immunology 2021Quote: Between 104 and 3.104 murine and human T cells were sorted from lymph nodes and tumors in TCL buffer (Qiagen) with 1% of β-mercaptoethanol ...
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bioRxiv - Cell Biology 2021Quote: The human VCP cDNA was reverse transcribed from total RNA by using Omniscript RT kit (Qiagen Japan, Tokyo, Japan) extracted from A549 cells (RIKEN Cell Bank ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA targets were only included if biochemically confirmed using human tissue or non-species specific methods (sourced from QIAGEN’s curated Ingenuity Knowledge Base[48] ...
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bioRxiv - Immunology 2021Quote: ... followed by amplification and quantification using RT2 Profiler Human Innate and Adaptive Immune Response 96-well Array (Qiagen, 330231) with RT2 SYBR Green ROX qPCR Mastermix (Qiagen ...
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bioRxiv - Microbiology 2021Quote: Bacterial DNA was extracted from human and mouse feces-derived bacterial supernatant using the DNeasy powersoil kit (Qiagen, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... or postmortem human cerebellar vermis samples using either the RNeasy Kit or RNeasy Fibrous Tissue Kit following the manufacturer’s protocols (QIAGEN). For tissue RNA extraction ...
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bioRxiv - Cancer Biology 2022Quote: ... The sequenced reads were mapped with the hg38 human genome and sequence analysis was done by the CLC genomics workbench (12.0.3, Qiagen Bioinformatics). Additionally ...
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bioRxiv - Microbiology 2022Quote: DNA was extracted from both NHP and human stool samples using the PowerFecal DNA Isolation Kit (Qiagen, Valencia, CA). Sequencing of the 16s small subunit ribosomal ribonucleic acid (SSU rRNA ...
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bioRxiv - Microbiology 2023Quote: ... Human DNA from whole cell extracts of A549 cells was purified using the DNeasy Blood and Tissue kit (Qiagen). RNAseA (Thermo ...
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bioRxiv - Microbiology 2023Quote: Total genomic DNA was extracted from human tissue and cyst fluid using the QIAamp Mini kit (Qiagen, Hilden, Germany). Extractions were performed per manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... Raw FASTQ reads were aligned to the GRCh38 human genome using CLC Genomics Workbench version 22.0.2 software (Qiagen, USA). Differential gene expression was determined using the built-in tool in CLC Genomics workbench ...
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bioRxiv - Physiology 2024Quote: RNA was extracted from human tissue derived organoids and neutrophils using the RNeasy® Plus Mini Kit (Qiagen, #74104) with cDNA prepared using a SuperScript™ VILO™ cDNA synthesis kit (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: ... Synchronized ring-stage parasites were transfected with 100 μg of purified plasmid DNA (PlasmidMidi Kit, Qiagen, Valencia, CA) by electroporation (310 V ...
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bioRxiv - Molecular Biology 2020Quote: ... Plasmid DNA was extracted using the Qiagen HiSpeed Maxi Kit (cat. no. 12662, Qiagen Inc, Valencia, CA, USA) following the vendor’s protocol.
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bioRxiv - Biochemistry 2020Quote: ... DNA constructs were amplified in Escherichia coli DH5α and purified using a Hi-Speed Plasmid Maxi Kit (Qiagen).
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bioRxiv - Molecular Biology 2019Quote: ... The band corresponding to closed-circular plasmid was excised and extracted with the QIAquick gel extraction kit (Qiagen). A second independent replicate was performed ...
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bioRxiv - Genomics 2019Quote: ... cultured overnight and extracted using Endo Free® Plasmid Maxi or QIAprep® Spin Miniprep (gRNAs) kits (QIAGEN). gRNAs sequence and locations are listed in Table S5.
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bioRxiv - Biochemistry 2020Quote: ... and plasmids were purified by use of a QIAprep Spin Miniprep Kit according to the manufacturer’s instructions (QIAGEN). The selected plasmids were purified by use of a QIAprep Spin Miniprep Kit according to the manufacturer’s instructions (QIAGEN) ...