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Citations for Qiagen :
1351 - 1400 of 1924 citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The plasmids of the final confirmed-positive clones were purified using the Maxiprep kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... and grown at 30°C or 37°C for the plasmid DNA preparation (Qiagen miniprep). The resulting plasmids were sequence-verified using Sanger sequencing (Genewiz).
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bioRxiv - Cancer Biology 2022Quote: ... plasmid DNA was isolated and purified using the Qiagen Maxi Prep kit (Qiagen Cat. #12963). Details of each shRNA plasmid constructs can be found in the Supplementary Table 8.
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bioRxiv - Microbiology 2022Quote: ... Plasmids containing amplicon inserts were purified by the QIAprep spin miniprep kit (Qiagen, Hilden, Germany) and then serially diluted ...
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bioRxiv - Microbiology 2021Quote: ... The plasmid harboring the mutation was further amplified and purified with EndoFree maxi kit (Qiagen) to be suitable for transfection into mammalian cells.
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bioRxiv - Developmental Biology 2020Quote: ... The pAct-Gal4 and pUAST-bab2CD plasmids were co-transfected using Effectene Transfection Reagent (QIAGEN) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... Plasmid extraction was performed using QIAprep Spin Miniprep Kit according to the manufacturer’s instructions (Qiagen).
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bioRxiv - Bioengineering 2020Quote: ... plasmid in transfected bacteria were amplified and extracted with Qiagen Maxiprep kit (Qiagen, Manchester, UK). HEK293FT cells (gifted by Dr ...
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bioRxiv - Evolutionary Biology 2022Quote: Extraction of the plasmids from the evolved populations was performed using the QIAprep Miniprep (Qiagen) on the QiaCube extraction platform from liquid culture from the frozen populations grown in LB Miller supplemented with 32mg/L of piperacillin ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... coli and then extracted and purified using a plasmid purification maxi kit (Qiagen, Valencia, CA). Purity was assessed by the ratio of absorbance at 260 and 280 nm ...
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bioRxiv - Biophysics 2020Quote: ... and used to isolate plasmid DNA using the QIAprep® Spin Miniprep kit from Qiagen and following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids were purified from overnight cultures with the QIAprep Spin Miniprep Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions.
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bioRxiv - Genomics 2021Quote: ... and plasmids were purified using QIAprep Spin Miniprep kit according to the manufacturer’s instructions (Qiagen). PCRs were performed to amplify and linearize the different NORAD fragments using the Pfu mix (#2021 ...
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bioRxiv - Immunology 2020Quote: ... Plasmid libraries were purified from bacterial transformants using the QIAprep Spin Miniprep kit (Qiagen, #27106). HDR templates were generated from plasmid libraries by PCR using primer pair RVL-127/128 and column-purified prior to transfection.
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bioRxiv - Cancer Biology 2019Quote: ... Single colonies were picked and expanded for plasmid DNA mini prep (Qiagen, Germantown, MA, USA). Correct insertion of shRNA sequences was confirmed by sequencing using the 5’ pSico-Eco-insert-seq 28 ...
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bioRxiv - Genetics 2021Quote: ... using custom or vector primers and plasmid DNA purified with the QIAprep Spin kit (Qiagen). Reads were assembled using MacVector software.
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bioRxiv - Synthetic Biology 2022Quote: ... The plasmid DNA was purified from the cultures using Qiaprep Spin Miniprep Kit (Qiagen, Germany) following manufacturer’s protocol ...
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bioRxiv - Physiology 2021Quote: ... Plasmid DNA was extracted from bacterial cultures using a QIAprep Spin Miniprep Kit (QIAGEN – 27104) and Sanger sequenced at the Dana-Farber/Harvard Cancer Center DNA Resource Core or Genewiz ...
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bioRxiv - Genetics 2021Quote: ... Chromosomal and plasmid DNA molecules were extracted with a DNeasy Blood and Tissue Kit (Qiagen) and a High Pure Plasmid Isolation Kit (Roche) ...
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bioRxiv - Genetics 2020Quote: ... Cells were transfected with 1.5ug of each plasmid DNA with the Effectene transfection kit (Qiagen). After 48 hours in transfection complex ...
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bioRxiv - Microbiology 2021Quote: ... Colonies were picked and plasmid DNA was isolated using the QIAprep Spin Miniprep Kit (Qiagen) and plasmids were sequenced at the Molecular Biology Resource Facility at the University of Tennessee ...
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bioRxiv - Microbiology 2021Quote: ... Plasmid DNA isolation was performed with a standard kit according to the manufacturer’s instructions (Qiagen). General microbiological manipulations ...
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bioRxiv - Microbiology 2020Quote: ... Plasmid DNA extraction from bacteria was performed using QIAprep Spin Miniprep Kit (Qiagen, Venlo, Netherlands). DNA was isolated from agarose bands with the NucleoSpin Extract II kit (MN ...
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bioRxiv - Genomics 2020Quote: ... plasmid DNA was extracted using the QIAprep Spin Miniprep standard protocol (Qiagen, cat. No. 27104). To validate the presence of the desired inserts ...
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bioRxiv - Evolutionary Biology 2022Quote: Plasmids of selected mutants were extracted and purified using the QIAprep Spin Miniprep Kit (Qiagen) according to the manufactures protocol ...
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bioRxiv - Genomics 2022Quote: ... Plasmids containing the PCR product were extracted with the QIAprep Spin Miniprep Kit (Qiagen, Germany). Plasmid products were Sanger sequenced by Macrogen Europe (Amsterdam ...
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bioRxiv - Immunology 2022Quote: ... Plasmid DNA of sPLA2 and Crypt1 was prepared using the QIAprep Spin Miniprep Kit (Qiagen) and used to generate standard curves (56) ...
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bioRxiv - Genetics 2024Quote: ... Minipreparations of plasmids was done using the QIAprep® Spin Miniprep Kit (QIAGEN, Hilden, Germany) and sequenced on both strands using pJET1.2-specific primers (Microsynth AG ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we isolated the plasmids using a Qiagen QIAprep Spin Miniprep kit (Qiagen, Germany, product #27104) as described by the manufacturer ...
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bioRxiv - Developmental Biology 2024Quote: ... restriction enzyme digests were performed after plasmids were isolated using a midiprep kit (QIAGEN, 27104). All riboprobes were generated from linearized plasmids and synthesized using the DIG RNA Labelling Kit (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... the DNA was purified following manufacturer’s instructions for the Qiagen Plasmid Maxi Kit (Qiagen, 12162).
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bioRxiv - Genomics 2023Quote: ... to remove the template plasmid and cleaned up using the RNeasy MinElute Kit (Qiagen, 74204). All constructs were polyadenylated using Escherichia coli poly(A ...
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bioRxiv - Microbiology 2024Quote: ... 1.5x105 Acanthamoeba castellanii cells were transfected with 6 μg of linearized plasmid using Polyfect (QIAGEN) in phosphate saline buffer (PBS) ...
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bioRxiv - Molecular Biology 2023Quote: ... and a second 200 ml overnight culture was purified using the Plasmid Maxi Kit (QIAGEN). The new plasmid is named pWPI- IFITM3.
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bioRxiv - Cell Biology 2023Quote: ... Cells were transfected with pGL1 or pGL2 cDNA expression plasmids using Effectene transfection reagent (Qiagen). In brief ...
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bioRxiv - Bioengineering 2023Quote: ... Plasmid purification of the overnight cultures was done with QIAGEN Miniprep Kit (QIAGEN, Hilden, Germany) and quantified with the dsDNA HS Qubit Kit (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... Ampicillin-resistant colonies were grown in liquid culture as a source of plasmid DNA (Qiagen QIAprep Spin Miniprep Kit ...
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bioRxiv - Molecular Biology 2023Quote: ... The plasmids that had been propagated were then isolated using the QIAgen Miniprep Kit (Qiagen). TAM motifs that were preferentially depleted were identified using amplicon sequencing of the TAM library plasmid ...
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bioRxiv - Synthetic Biology 2023Quote: ... Routine isolation of the plasmids was performed using the Qiaprep Spin Miniprep kit from Qiagen, USA ...
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bioRxiv - Systems Biology 2023Quote: ... and plasmid DNA was purified from overnight cultures using the QIAprep Spin Miniprep Kit (Qiagen). Proper annealing of sgRNA into the lentiCRISPRv2 backbone was confirmed by Sanger sequencing (Azenta ...
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bioRxiv - Cell Biology 2023Quote: All constructs for bacterial protein expression were cloned in the pQE plasmid (Qiagen, Valencia, CA). WT and mutant TTC1 were cloned downstream of a His14-bdSUMO-GFP tag ...
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bioRxiv - Biochemistry 2023Quote: ... The plasmid DNA was purified from the cultures using Qiaprep Spin Miniprep Kit (Qiagen, Germany), digested by appropriate restriction enzymes ...
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bioRxiv - Cancer Biology 2022Quote: ... coli following ampicillin selection using the QIAGEN Plasmid Purification Midi-Prep Kit (QIAGEN, Hilden, Germany) and reconstituted in sterile Tris-EDTA (TE ...
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bioRxiv - Immunology 2023Quote: ... plasmid preparation and DNA gel extraction were performed using nucleotide purification kits purchased from Qiagen or BioBasics ...
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bioRxiv - Molecular Biology 2023Quote: ... and a second 200 ml overnight culture was purified using the Plasmid Maxi Kit (QIAGEN). The new plasmid is named pHW2000-PA-WSN-mScarlet- codon-optimized.
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bioRxiv - Microbiology 2023Quote: ... Individual colonies were screened for the presence of the correct plasmids after miniprep (Qiagen kit) extraction from overnight cultures using restriction enzyme digest and Sanger sequencing ...
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bioRxiv - Evolutionary Biology 2023Quote: Plasmids were collected from each culture aliquot using QIAprep spin miniprep kits (Qiagen, Hilden, Germany). The pool of barcodes was amplified as previously described (2) ...
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmid DNA was extracted from this culture using the QIAprep Spin Miniprep Kit (Qiagen, 27104). Finally ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmids were purified from overnight cultures using the QIAprep Spin MiniPrep Kit (Qiagen Cat #: 27106). Proper assembly of the construct was screened for by restriction digest of the purified plasmids followed by gel electrophoresis ...
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bioRxiv - Cancer Biology 2023Quote: ... The clear lysate was transferred to a QIAilter Catridge (From QIAGEN Plasmid Plus Midi Kit) and incubated at room temperature for 10 mins ...