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Citations for Qiagen :
1251 - 1300 of 1799 citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: ... The crude product is further purified by EndoFree® Plasmid Maxi Kit (Qiagen, Cat. # 12362), to harvest the target ssDNA and remove hairpin adapters ...
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bioRxiv - Microbiology 2020Quote: ... The general protocol involved plasmid extraction using the QIAprep spin mini-prep kit (Qiagen, UK). 1 µg of plasmid was mixed with 10 U of the corresponding restriction enzymes and the corresponding amount of 1X restriction buffer in final 50 µL volume (New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: ... Enrichment of clones was followed by plasmid purification using the QIAprep Spin Miniprep Kit (Qiagen). The resulting plasmid DNA yield was measured by absorbance spectroscopy (OD260nm) ...
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bioRxiv - Genomics 2020Quote: ... S2 cells were transfected with 1 ug plasmid DNA using the Effectene reagent kit (Qiagen). The plasmid DNA which contains cDNA of FLAG-tagged ECDs are under the metallothionein promoter control ...
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bioRxiv - Cancer Biology 2020Quote: ... The plasmid DNA was extracted from each culture using the QIAprep Spin Miniprep Kit (Qiagen) and sequenced using the Sanger method to guarantee the correctness of the amplified sequences.
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bioRxiv - Microbiology 2019Quote: ... Plasmid DNA from the transconjugants was extracted using QIAprep Spin Miniprep Kit (Qiagen, Hilden, Germany) following the manufacturer's recommendations.
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bioRxiv - Microbiology 2019Quote: ... Selected colonies were used to extract plasmid DNA (QIAprep Spin Miniprep Kit; Qiagen, Hilden, Germany). Introduction of mutations were confirmed by Sanger sequencing ...
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Elizabethkingia anophelis response to iron stress: physiologic, genomic, and transcriptomic analysesbioRxiv - Microbiology 2019Quote: ... and plasmid DNA was purified with the QIAprep spin miniprep kit (QIAGEN, Germantown, MD, USA). PCR amplifications were done with the Failsafe PCR system (Epicenter Technology ...
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bioRxiv - Synthetic Biology 2019Quote: ... Plasmids were extracted from E.coli JM109 transformed cells using the QIAprep Spin Miniprep Kit (Qiagen) and sequence verified by Sanger sequencing using the BigDye Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2021Quote: ... after which we pelleted cultures by centrifugation and used the EndoFree Plasmid Maxi Kit (Qiagen) to isolate plasmid according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2020Quote: ... The first step was to produce a plasmid by ligation of two gel extracted (QIAGEN) DNA fragments ...
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bioRxiv - Neuroscience 2020Quote: ... Promega) and dominant-negative REST (DN-REST)70 or GFP expression plasmids using Polyfect (Qiagen). Luciferase activities were measured 24 hours later ...
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bioRxiv - Biochemistry 2020Quote: ... 37°C overnight followed by plasmid DNA purification using a QIAprep Spin Miniprep Kit (Qiagen).
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bioRxiv - Plant Biology 2021Quote: ... the solution was loaded onto a plasmid DNA extraction column (QIAprep Spin Miniprep Kit; Qiagen), and went through several washes before elution of the RNA ...
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Dissemination of linezolid-resistance through sex pheromone plasmid transfer in Enterococcs faecalisbioRxiv - Microbiology 2019Quote: ... Genomic DNA was extracted from overnight culture using the Qiagen Plasmid Midi Kit (Qiagen, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The plasmids of the final confirmed-positive clones were purified using the Maxiprep kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... and grown at 30°C or 37°C for the plasmid DNA preparation (Qiagen miniprep). The resulting plasmids were sequence-verified using Sanger sequencing (Genewiz).
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bioRxiv - Cancer Biology 2022Quote: ... plasmid DNA was isolated and purified using the Qiagen Maxi Prep kit (Qiagen Cat. #12963). Details of each shRNA plasmid constructs can be found in the Supplementary Table 8.
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bioRxiv - Microbiology 2022Quote: ... Plasmids containing amplicon inserts were purified by the QIAprep spin miniprep kit (Qiagen, Hilden, Germany) and then serially diluted ...
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bioRxiv - Microbiology 2021Quote: ... The plasmid harboring the mutation was further amplified and purified with EndoFree maxi kit (Qiagen) to be suitable for transfection into mammalian cells.
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bioRxiv - Developmental Biology 2020Quote: ... The pAct-Gal4 and pUAST-bab2CD plasmids were co-transfected using Effectene Transfection Reagent (QIAGEN) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... Plasmid extraction was performed using QIAprep Spin Miniprep Kit according to the manufacturer’s instructions (Qiagen).
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bioRxiv - Bioengineering 2020Quote: ... plasmid in transfected bacteria were amplified and extracted with Qiagen Maxiprep kit (Qiagen, Manchester, UK). HEK293FT cells (gifted by Dr ...
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bioRxiv - Evolutionary Biology 2022Quote: Extraction of the plasmids from the evolved populations was performed using the QIAprep Miniprep (Qiagen) on the QiaCube extraction platform from liquid culture from the frozen populations grown in LB Miller supplemented with 32mg/L of piperacillin ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... coli and then extracted and purified using a plasmid purification maxi kit (Qiagen, Valencia, CA). Purity was assessed by the ratio of absorbance at 260 and 280 nm ...
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bioRxiv - Biophysics 2020Quote: ... and used to isolate plasmid DNA using the QIAprep® Spin Miniprep kit from Qiagen and following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids were purified from overnight cultures with the QIAprep Spin Miniprep Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions.
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bioRxiv - Genomics 2021Quote: ... and plasmids were purified using QIAprep Spin Miniprep kit according to the manufacturer’s instructions (Qiagen). PCRs were performed to amplify and linearize the different NORAD fragments using the Pfu mix (#2021 ...
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bioRxiv - Immunology 2020Quote: ... Plasmid libraries were purified from bacterial transformants using the QIAprep Spin Miniprep kit (Qiagen, #27106). HDR templates were generated from plasmid libraries by PCR using primer pair RVL-127/128 and column-purified prior to transfection.
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bioRxiv - Cancer Biology 2019Quote: ... Single colonies were picked and expanded for plasmid DNA mini prep (Qiagen, Germantown, MA, USA). Correct insertion of shRNA sequences was confirmed by sequencing using the 5’ pSico-Eco-insert-seq 28 ...
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bioRxiv - Genetics 2021Quote: ... using custom or vector primers and plasmid DNA purified with the QIAprep Spin kit (Qiagen). Reads were assembled using MacVector software.
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bioRxiv - Synthetic Biology 2022Quote: ... The plasmid DNA was purified from the cultures using Qiaprep Spin Miniprep Kit (Qiagen, Germany) following manufacturer’s protocol ...
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bioRxiv - Physiology 2021Quote: ... Plasmid DNA was extracted from bacterial cultures using a QIAprep Spin Miniprep Kit (QIAGEN – 27104) and Sanger sequenced at the Dana-Farber/Harvard Cancer Center DNA Resource Core or Genewiz ...
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bioRxiv - Genetics 2021Quote: ... Chromosomal and plasmid DNA molecules were extracted with a DNeasy Blood and Tissue Kit (Qiagen) and a High Pure Plasmid Isolation Kit (Roche) ...
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bioRxiv - Genetics 2020Quote: ... Cells were transfected with 1.5ug of each plasmid DNA with the Effectene transfection kit (Qiagen). After 48 hours in transfection complex ...
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bioRxiv - Microbiology 2021Quote: ... Colonies were picked and plasmid DNA was isolated using the QIAprep Spin Miniprep Kit (Qiagen) and plasmids were sequenced at the Molecular Biology Resource Facility at the University of Tennessee ...
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bioRxiv - Microbiology 2021Quote: ... Plasmid DNA isolation was performed with a standard kit according to the manufacturer’s instructions (Qiagen). General microbiological manipulations ...
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bioRxiv - Microbiology 2020Quote: ... Plasmid DNA extraction from bacteria was performed using QIAprep Spin Miniprep Kit (Qiagen, Venlo, Netherlands). DNA was isolated from agarose bands with the NucleoSpin Extract II kit (MN ...
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bioRxiv - Genomics 2020Quote: ... plasmid DNA was extracted using the QIAprep Spin Miniprep standard protocol (Qiagen, cat. No. 27104). To validate the presence of the desired inserts ...
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bioRxiv - Evolutionary Biology 2022Quote: Plasmids of selected mutants were extracted and purified using the QIAprep Spin Miniprep Kit (Qiagen) according to the manufactures protocol ...
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bioRxiv - Genomics 2022Quote: ... Plasmids containing the PCR product were extracted with the QIAprep Spin Miniprep Kit (Qiagen, Germany). Plasmid products were Sanger sequenced by Macrogen Europe (Amsterdam ...
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bioRxiv - Immunology 2022Quote: ... Plasmid DNA of sPLA2 and Crypt1 was prepared using the QIAprep Spin Miniprep Kit (Qiagen) and used to generate standard curves (56) ...
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bioRxiv - Molecular Biology 2023Quote: ... and a second 200 ml overnight culture was purified using the Plasmid Maxi Kit (QIAGEN). The new plasmid is named pWPI- IFITM3.
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bioRxiv - Cell Biology 2023Quote: ... Cells were transfected with pGL1 or pGL2 cDNA expression plasmids using Effectene transfection reagent (Qiagen). In brief ...
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bioRxiv - Bioengineering 2023Quote: ... Plasmid purification of the overnight cultures was done with QIAGEN Miniprep Kit (QIAGEN, Hilden, Germany) and quantified with the dsDNA HS Qubit Kit (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... Ampicillin-resistant colonies were grown in liquid culture as a source of plasmid DNA (Qiagen QIAprep Spin Miniprep Kit ...
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bioRxiv - Molecular Biology 2023Quote: ... The plasmids that had been propagated were then isolated using the QIAgen Miniprep Kit (Qiagen). TAM motifs that were preferentially depleted were identified using amplicon sequencing of the TAM library plasmid ...
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bioRxiv - Synthetic Biology 2023Quote: ... Routine isolation of the plasmids was performed using the Qiaprep Spin Miniprep kit from Qiagen, USA ...
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bioRxiv - Systems Biology 2023Quote: ... and plasmid DNA was purified from overnight cultures using the QIAprep Spin Miniprep Kit (Qiagen). Proper annealing of sgRNA into the lentiCRISPRv2 backbone was confirmed by Sanger sequencing (Azenta ...
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bioRxiv - Cell Biology 2023Quote: All constructs for bacterial protein expression were cloned in the pQE plasmid (Qiagen, Valencia, CA). WT and mutant TTC1 were cloned downstream of a His14-bdSUMO-GFP tag ...