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Citations for Qiagen :
1501 - 1550 of 1799 citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... The corresponding band was excised from the gel and the plasmid extracted using Qiaquick gel extraction kit (Qiagen). The forward and reverse phosphorylated primers (Sigma Chemical ...
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bioRxiv - Synthetic Biology 2019Quote: ... and plasmid was prepped from each batch separately using QIAprep Spin Miniprep kits (QIAGEN, Cat No./ID: 27104) and then pooled ...
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bioRxiv - Neuroscience 2020Quote: Previously cloned CADPS2 plasmids (Accession: DV955943) were grown from bacterial stock and collected via miniprep columns (Qiagen 27104). CADPS2 was verified for sequence identity and orientation using Sanger sequencing services provided by Eton Biosciences or GeneWiz ...
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bioRxiv - Synthetic Biology 2022Quote: ... Plasmids were extracted from the cultures using the QIAprep Spin Miniprep Kit (QIAGEN, Hilden, Germany, catalogue no. 27106) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... plasmids and growth conditions) were digested using NotI restriction enzyme and purified using QiAquick PCR purification kit (Qiagen). One microgram of cpsA-gfp ...
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bioRxiv - Biochemistry 2019Quote: ... DNA constructs were amplified in Escherichia coli DH5α and purified using a Hi-Speed Plasmid Maxi Kit (Qiagen).
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bioRxiv - Bioengineering 2019Quote: ... The selected colony with confirmed insertion sequence was cultivated and DNA extracted (Plasmid Plus Midi Kit, Qiagen, Netherlands) and then excised from the gel (Sigma-Aldrich x-tracta Gel Extraction Tool ...
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bioRxiv - Bioengineering 2019Quote: ... The selected colony with confirmed insertion sequence was cultivated and DNA extracted (Plasmid Plus Midi Kit, Qiagen, Netherlands) and then excised from the gel (Sigma-Aldrich x-tracta Gel Extraction Tool ...
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bioRxiv - Genomics 2019Quote: ... Library vectors with insert (pJDrcEPP_lib_minPLuc2) were isolated from the remainder transformants using Qiagen Plasmid Plus Maxi Kit (Qiagen).
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bioRxiv - Molecular Biology 2021Quote: A plasmid containing the modified Widom 603–42 sequence31 was purified with a QIAquick PCR Purification Kit (Qiagen) and used as the template for the amplification.
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bioRxiv - Biophysics 2019Quote: ... The SpyTag-(Cpa)4-SpyTag and the HaloTag-SpyCatcher constructs were cloned into the expression plasmid pQE80L (Qiagen). Protein expression and purification was done as described elsewhere36 ...
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bioRxiv - Neuroscience 2021Quote: ... Viral DNA and standard plasmid DNA were extracted using the QIAGEN DNeasy Blood and Tissue kit (QIAGEN #69504). qPCR reactions of serial-dilutions of the DNA samples were prepared using TaqMan Universal PCR Master Mix (ThermoFisher #4304437 ...
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bioRxiv - Biochemistry 2021Quote: DNA constructs were amplified in Escherichia coli DH5α and purified using a Hi-Speed Plasmid Maxi Kit (Qiagen).
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bioRxiv - Cell Biology 2022Quote: ... Plasmids were amplified in Escherichia coli (XL-Blue) extracted by alkaline lysis and purified by column separation (Qiagen). The integrity was confirmed by gel electrophoresis in 0.8% agarose ...
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bioRxiv - Cell Biology 2022Quote: ... PLA was performed on S2 cells that were transfected with the following plasmids using Effectene Transfection Reagent (QIAGEN): control Myc ...
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bioRxiv - Immunology 2022Quote: ... Plasmid extraction was carried out with Qiagen QIApep® spin miniprep kit following manufacturer’s instructions (QIAGEN Ltd, UK). Inserts were confirmed by EcoRI restriction digestion and gel electrophoresis ...
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bioRxiv - Molecular Biology 2022Quote: ... coli grown for 16 h in 250 ml of 2×YT medium using the Plasmid Maxi Kit (QIAGEN) in accordance with the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... Plasmids were prepared by mini or maxi preps using commercially available kits (Qiagen, UK and Promega, UK, respectively) following manufacturers’ instructions ...
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bioRxiv - Biophysics 2023Quote: ... we isolated cosmid-i95 or pBS-T7A1-parS via a midiprep using a Qiafilater plasmid midi kit (Qiagen). We digested the cosmid-i95 for 2 h at 37°C using SpeI-HF restriction enzyme (New England Biolabs ...
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bioRxiv - Biochemistry 2023Quote: ... and grown in LB medium (ampicillin 50 μg mL−1) and plasmid purified (miniprep kit Qiagen, Hilden, Germany). Mutations were confirmed by DNA sequencing (Eurofins Genomics ...
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bioRxiv - Microbiology 2023Quote: ... and plasmids containing the full-length reverse transcriptase gene were isolated with the QIAprep Spin Miniprep kit (Qiagen). Primers ABR-RT-OF and ABR-RT-OR2 were used to sequence the 5’ and 3’ ends of reverse transcriptase ...
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bioRxiv - Molecular Biology 2023Quote: ... All plasmids were purified with a Midiprep kit (ZymoPURE, #D413) or a QIAprep spin miniprep kit (QIAGEN, #27104) and verified by DNA sequencing prior to use ...
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bioRxiv - Biochemistry 2023Quote: ... Negatively supercoiled pHOT-1 DNA was purified from DH5α utilizing a Qiagen Plasmid Midi Kit (Qiagen, Hilden, Germany).
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bioRxiv - Biochemistry 2023Quote: ... Plasmids were isolated from the inoculum and the m9sa cultures following selection using a miniprep kit (Qiagen, Inc.).
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bioRxiv - Biophysics 2023Quote: MCF10A-ER-src cells were transiently transfected with the CAV1 overexpressing plasmid using Effectene transfection reagent (301425, Qiagen). One day before transfection ...
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bioRxiv - Cancer Biology 2023Quote: ... Plasmid DNA from ~ 20 positive clones for each cell line was isolated using QIAprep Spin Miniprep Kit (Qiagen) and the cloned rDNA promoter region was sequenced using Sanger sequencing ...
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bioRxiv - Plant Biology 2023Quote: All plasmid DNA isolation from bacterial cells was performed using the QIAprep Spin Miniprep Kit (Qiagen; Hilden, Germany) according to the manufacturer’s information ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA was isolated using either QIAGEN Plasmid Plus Midi Kit or 28 QiaPrep Spin Miniprep Kit (Qiagen, 27104).
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmid DNA was extracted from 5 mL of this culture using the QIAprep Spin Miniprep Kit (Qiagen, 27104). The remaining 95 mL of culture was combined with 40.7 mL 50% glycerol (v/v) ...
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmid DNA was extracted from 5 mL of this culture using the QIAprep Spin Miniprep Kit (Qiagen, 27104). Glycerol was added to the remaining culture to a final concentration of 15% (v/v ...
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bioRxiv - Microbiology 2023Quote: ... coli and the plasmid DNA was extracted using the pipetting Bio Robot Universal System (Qiagen cat. no. 9001094) and the QIAprep 96 plus BioRobot kit (Qiagen cat ...
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bioRxiv - Developmental Biology 2023Quote: ... 200 ng psiCHECK-2 reporter plasmid were co-transfected with 5 µl miScript miRNA mimics (Qiagen; CatNo. 219600) using 4 µl Lipofectamine 2000 Transfection Reagent (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... ∼8 ml PCR product (azide-DNA:2xbiotin-DNA = 1:1) was purified using HiSpeed Plasmid Maxi kit (Qiagen) in 1 ml NaHCO3 (pH 8.3) ...
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bioRxiv - Genomics 2024Quote: ... each 50 mL midiprep culture was spun down for 30 minutes at 4,300g and plasmid DNA isolated using a midiprep kit according to the manufacturer’s instructions (Qiagen).
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bioRxiv - Developmental Biology 2024Quote: ... Inserts were PCR amplified using M13 primers and template plasmid was digested with DpnI before PCR purification (Qiagen). Digoxygenin labeled antisense probes were synthesized using SP6 or T7 RNA polymerase (Promega) ...
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bioRxiv - Developmental Biology 2024Quote: ... The plasmid DNAs were linearized by a restriction enzyme and purified with the Qiaquick Gel Extraction Kit (Qiagen) before microinjection ...
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bioRxiv - Microbiology 2024Quote: The isolation of DNA (GeneEluteTM Bacterial Genomic DNA, Sigmal-Aldrich) or plasmids (QIAprep® Spin Miniprep Kit, Qiagen) was performed applying commercial kits ...
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bioRxiv - Cell Biology 2020Quote: ... The cDNA (200 ng) was used for quantitative PCR with the Human Cellular Senescence RT2 Profiler™ PCR Array (Qiagen) containing 84 key genes involved in the initiation and progression of the biological process causing cells to lose the ability to divide ...
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bioRxiv - Immunology 2021Quote: RNA was extracted from the cell pellets of human primary male and female PT cells using the RNAeasy Mini Kit (Qiagen). After quantifying RNA concentration in a Nanodrop instrument (Thermo) ...
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bioRxiv - Cancer Biology 2019Quote: ... was isolated from human PanNET specimens or cells grown on 6-cm or 10-cm plates using RNeasy mini kit (Qiagen) containing gDNA eliminator spin columns ...
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bioRxiv - Molecular Biology 2019Quote: Preliminary screening for the presence of serum exosomal miRNAs was determined using a miScript human miFinder PCR array (MIHS-001Z, Qiagen). RNA was converted to cDNA using miScript RT kit (218060) ...
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bioRxiv - Plant Biology 2019Quote: ... three replicates of 25 to 30 pooled egg cells were used for total RNA extraction according to the “Purification of total RNA from animal and human cells” protocol of the RNeasy Plus Micro Kit (Qiagen). In brief ...
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bioRxiv - Genetics 2020Quote: ... Analyses of HTT CAG repeat size in both HttQ111 mice and in patient fibroblasts was performed by PCR using human-specific HTT primers and Taq PCR Core Kit with Q solution (Qiagen), as previously described (17 ...
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bioRxiv - Bioengineering 2020Quote: ... The prepared cDNA was preamplified using the RT2 PreAMP Primer Mix for Human and Mouse PCR Array (Qiagen, PBH-181Z). cDNA was analyzed by RT-qPCR using a Qiagen RT2 profiler custom panel (Qiagen ...
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bioRxiv - Microbiology 2021Quote: Total RNA of 1-2 x 106 human macrophages (2-4 biological replicates/ macrophage donors per condition, see Table S17) was isolated using RNeasy extraction kit (Qiagen) including DNAse treatment according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Synthetized cDNA was subjected to a PCR array specific for the human antiviral response (RT² Profiler PCR array – PAHS-122Z, SA Biosciences, Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... was used for human ISG15 and ISG56 and mouse genes including Gapdh as the housekeeping gene on the Rotor-Gene Q 5plex (Qiagen). RT-qPCR primers and probes are listed in the supplementary table 2.
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bioRxiv - Microbiology 2021Quote: ... mRNA-Seq FASTQ reads were mapped to the human reference genome (Homo sapiens v81; hg38) using default options on CLC Genomics Workbench 11 (Qiagen). Total gene reads (with at least 1 read count ...
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bioRxiv - Genomics 2020Quote: RNA isolation for all the 248 human subjects were performed using QIAamp Viral RNA Mini Kit (Qiagen, Cat. No. 52906). The isolated RNA was subjected to qPCR for determining viral load by Ct values ...
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bioRxiv - Neuroscience 2021Quote: Total RNA was extracted from human fibroblasts as well as iNs from the same lines using the miRNeasy kit (Qiagen) followed by Universal cDNA synthesis kit (Fermentas) ...