Labshake search
Citations for Qiagen :
1601 - 1650 of 1799 citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... HEK-293T cells were next transfected with pMMP vectors together with retrovirus packaging plasmids using Effectene transfection reagents (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: Cells (200,000 for HeLa and hiPSC-CMs and 500,000 for NRCMs) were seeded on glass cover slips and transfected with sensor plasmids using Effectene (Qiagen) (HeLa ...
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bioRxiv - Immunology 2020Quote: ... Freestyle 293F (ThermoFisherScientific, Cat#R79007) cell was transfected with purified miniprep plasmid DNA (QIAgen minipreps kit, Qiagen, Cat#29107) as described (Lipofectamine™ RNAiMAX Transfection Reagent ...
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bioRxiv - Immunology 2020Quote: ... Freestyle 293F (ThermoFisherScientific, Cat#R79007) cell was transfected with purified miniprep plasmid DNA (QIAgen minipreps kit, Qiagen, Cat#29107) as described (Lipofectamine™ RNAiMAX Transfection Reagent ...
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bioRxiv - Systems Biology 2022Quote: ... the bacteria cells were scraped and centrifuged at 3,000g for 10 min and the plasmids were then extracted (Qiagen). Cloning of individual sgRNA base editor construct was based on similar procedure with reduced reagent input.
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bioRxiv - Molecular Biology 2023Quote: ... Cultures were normalised to cell density and DNA was isolated using the Plasmid Maxi Kit (Qiagen, Cat. No. 12163) according to manufacturer’s directions except that all steps and reagents ...
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bioRxiv - Genetics 2023Quote: ... Bacterial cells were cultured overnight in liquid and the final libraries were extracted using QIAGEN Plasmid Maxi Kit (QIAGEN). The libraries were quantified and subpools combined in 1:4.76 molar ratio to get the final library containing 5,760 gRNAs.
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bioRxiv - Biochemistry 2023Quote: ... The plasmid was transformed into stbl3 competent E.Coli and DNA was harvested from 200 mL growth media using an endotoxin free plasmid purification kit (Qiagen).
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bioRxiv - Synthetic Biology 2023Quote: ... Colonies were cultured in 2x YT liquid media overnight and plasmids were extracted using QIAprep Spin Miniprep kit (Qiagen).
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bioRxiv - Molecular Biology 2023Quote: ... EGFP-ZMYM2(WT) (pAS4329) or EGFP-ZMYM2(SIMmut) (pAS4330) plasmids using Polyfect Transfection Reagent (Qiagen, Cat. No. ID: 301105) for 24 hours ...
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bioRxiv - Immunology 2023Quote: ... and plasmid DNA from 200-500 µl of the cell suspension was purified using QIAprep Spin Miniprep Kit (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2023Quote: ... The supernatant was recovered and plasmid DNA was purified following the standard QIAprep Spin Miniprep Kit (QIAGEN, Hilden, Germany) protocol and eluted in 50 μl EB buffer.
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bioRxiv - Cell Biology 2023Quote: ... Overnight cultures were pelleted at 3,000g for 10 min and plasmid DNA was purified using a Qiagen miniprep kit (27106, Qiagen). Sequences were verified by Sanger sequencing (Eton Bioscience Inc).
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bioRxiv - Cancer Biology 2023Quote: ... and 9-10 mL of Buffer BB (1/3 of the lysate volume, From QIAGEN Plasmid Plus Midi Kit) was added ...
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bioRxiv - Cancer Biology 2023Quote: ... Crude eccDNA was then eluted by using 100 µL of 0.1x EB buffer (From QIAGEN Plasmid Plus Midi Kit). For each microgram crude eccDNA we spiked in 1 ng pUC1932 (was a gift from Joachim Messing ...
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bioRxiv - Biochemistry 2023Quote: ... An endotoxin-free preparation of the cloned vector was made using the EndoFree Plasmid Maxi Kit (Qiagen, Hilden, DE).
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bioRxiv - Biochemistry 2023Quote: ... Cx43 constructs with a stop codon in the eGFP-NI plasmid were purified with QIAprep Midi Prep Kit (Qiagen) suitable for Lipofectamine2000 (Invitrogen/Thermofisher ...
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bioRxiv - Genomics 2024Quote: ... each 50 mL midiprep culture was spun down for 30 minutes at 4,300 x g and plasmid DNA isolated using a midiprep kit according to the manufacturers instructions (Qiagen).
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bioRxiv - Cell Biology 2023Quote: ... Linearized plasmids were purified using a QIAquick PCR purification kit according to the manufacturer’s instructions (28106, Qiagen, Hilden, Germany). Templates for CLN3 RNA synthesis were in the same manner but using CLN3 specific primers (for ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were seeded in 24-well plates and transfected with the desired plasmids using Effectene Transfection Reagent (#301427, QIAGEN) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... was used to amplify DNA fragment except for PCR verification of plasmids or strains for which Taq polymerase (Qiagen) was used ...
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bioRxiv - Microbiology 2024Quote: ... The viral RNA product was then treated by DNase to remove the carry-over plasmid DNA (RNeasy Kit, Qiagen). cDNA of the virus genome was generated by reverse transcription with random hexamer as the primer (SuperScript III ...
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bioRxiv - Bioengineering 2020Quote: ... A Qiagen RT2 SYBR Green master mix with validated qPCR human primers (for HUVECs and BeWo) or mouse primers (for N27 cells) from Qiagen (Frederick) were used to determine relative magnitudes of gene-expression levels using RT-PCR ...
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bioRxiv - Molecular Biology 2020Quote: ... The human U6 primer used in this experiment was included in the miScript Primer assay kit (Qiagen Inc., Germantown, MD, #218300).
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bioRxiv - Molecular Biology 2022Quote: ... FAF1 (Uniprot identifier Q9UNN5-1) and UBXN7 (Uniprot identifier O94888-1) were amplified from XpressRef Universal Total human RNA (QIAGEN, 338112) by RT-PCR (TaKaRa ...
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bioRxiv - Developmental Biology 2021Quote: ... The expression levels of the mRNAs were quantified using Human Embryonic Stem Cell RT2 Profile™ PCR Array (Cat. PAHS-081, Qiagen) and RT2 SYBR Green qPCR Master Mix (Cat ...
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bioRxiv - Developmental Biology 2021Quote: RNA was isolated from a 23 week human fetal heart using Trizol-based dissociation followed by the RNEasy Mini Kit (Qiagen #74104). cDNA was created from this RNA using the iScript Reverse Transcription Supermix (Bio-Rad #1708840) ...
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bioRxiv - Systems Biology 2022Quote: DNA was isolated from human buffy coat samples at the Crimson Core facility (Mass General Brigham) using the QIAamp DNA Blood Mini Kit (Qiagen 69504) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Reference plasmids were serially diluted in either PBS buffer or heat-inactivated normal human serum (NHS) and re-extracted using the QIAamp® DNA Blood Mini Kit (Qiagen) per recommended protocol for serum ...
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bioRxiv - Cancer Biology 2020Quote: Flag-tagged NF1-GRD (aminoacids 1131-1534) was amplified by PCR from human cortical tissue (epilepsy patient) and first cloned in the pDRIVE vector (Qiagen #231124). Primers are listed in Supplementary Table 5 ...
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bioRxiv - Immunology 2021Quote: ... and ImmunoCult™ Human Treg Differentiation Supplement (StemCell TechnoRNA was isolated at set time points after activation using the RNeasy Mini (Qiagen). cDNA was generated using the High-Capacity RNA-to-cDNA Kit (ThermoFisher) ...
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bioRxiv - Molecular Biology 2022Quote: Genomic DNA was extracted from human cell lines of WI-38 and MED13LS1497F using DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany) according to manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2022Quote: ... Exosomal miRNAs were profiled using Human Serum/Plasma miRCURY LNA miRNA PCR array (Qiagen, #YAHS-106Y, Plate Format: 2 × 96-well).
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bioRxiv - Microbiology 2023Quote: RNA was extracted from a SARS-CoV-2 Omicron BA.1 clinical isolate (SARS-CoV-2/human/USA/CA-CDC-4358237-001/2021) (GenBank: OM264909.1) using the QIAamp Viral RNA kit (Qiagen, Hilden, Germany). The complete wild-type Omicron genome was then reverse transcribed via RT-PCR with SuperScript™ IV First-Strand Synthesis System (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... 1 µg of RNA isolated from human islets or 10 µL of exosomal RNA was reverse transcribed using the miRScript II kit (Qiagen, Germany), following manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... RT PCR was performed on a Roche LightCycler 96 using the The RT² Profiler™ PCR Array Human Cell Death PathwayFinder (PAHS-212Z, Qiagen)w ...
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bioRxiv - Neuroscience 2023Quote: ... The association of the differentially expressed proteins with specifically dysregulated regulatory/metabolic networks in OT human samples was analyzed using QIAGEN’s Ingenuity Pathway Analysis (IPA; QIAGEN Redwood City). This software calculates significance values (p-values ...
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bioRxiv - Molecular Biology 2024Quote: ... genomic DNA was extracted from the proteinase K-digested tail tips of mouse or peripheral blood mononuclear cell (PBMC) of human donors using DNeasy blood tissue kit (Qiagen, #69506). 25 ng of genomic DNA was subjected to bisulfite conversion using a commercial kit (Zymo Research ...
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bioRxiv - Biophysics 2021Quote: ... The mutagenesis plasmid pGJJ055 was digested with HindIII-HF and AvrII and purified with the MinElute PCR Purification Kit (QIAGEN). The GRB2 mutagenesis plasmid (pGJJ057 ...
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bioRxiv - Cell Biology 2019Quote: ... insertion of the gRNA was confirmed by gel electrophoresis of PCR products and the plasmid was purified using the QiaQuick Gel Extraction kit (Qiagen) following provider’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Cyp6a8-pOT2 and Cyp6d5-pOT2 plasmids were linearized overnight and purified into RNAse-free water using the MinElute Reaction Cleanup Kit (Qiagen). Labeled S and AS probes were generated using the DIG RNA Labeling Kit (SP6/T7 ...
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bioRxiv - Molecular Biology 2021Quote: ... Five colonies with the correct sized insert were inoculated into liquid LB supplemented with spectinomycin and chloramphenicol and grown overnight at 37°C for plasmid extraction using a QIAprep Spin Miniprep Kit (Qiagen). The plasmid constructs were confirmed by Sanger sequencing ...
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bioRxiv - Genomics 2020Quote: ... and a library representation of >200 colonies per guide was determined prior to CRISPR library pool plasmid purification by Endofree Maxi (Qiagen). Coverage of the 818 guide library for Perturb-CITE-Seq as well as the extended control library was confirmed by PCR amplification of the guide sequence from the final library pool and deep sequencing on an Illumina MiSeq.
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bioRxiv - Genetics 2019Quote: ... After 16 hrs in a 37°C shaker bacteria were harvested and plasmid DNA was isolated using a Megaprep kit (Qiagen). ITR integrity was confirmed by digestion with XmaI as well as with AhdI ...
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bioRxiv - Microbiology 2019Quote: ... Genes were predicted using Prokka (78) and plasmids were annotated and visualized via CLC Sequence Viewer (v8.0.0) (Qiagen, Aarhus, Denmark). For clade-to-clade chromosome comparisons ...
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bioRxiv - Genetics 2020Quote: ... Final CRE-reporter plasmids were isolated using Qiagen miniprep columns and were further purified on a Qiagen PCR purification column (Qiagen), and diluted to 50 ng/ml with nuclease free water ...
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bioRxiv - Bioengineering 2020Quote: Plasmid isolation from yeast was performed according to a user-developed protocol from Michael Jones (protocol PR04, Isolation of plasmid DNA from yeast, QIAGEN) using a QIAprep Spin Miniprep kit (QIAGEN ...
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bioRxiv - Bioengineering 2020Quote: ... the resulting lawns were scraped off the agar plates and the plasmid libraries were extracted using a QIAprep Spin Miniprep Kit (QIAGEN). The plasmid libraries were subsequently used for yeast transformation.
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bioRxiv - Bioengineering 2020Quote: ... The PCR fragments were incubated with DpnI for 2 h at 37°C to degrade the template plasmids before being purified using a PCR purification kit (Qiagen). The purified PCR fragments were digested overnight at 37°C using NheI and XhoI and ligated overnight at 16°C to backbone vector pYZ125 ...
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bioRxiv - Microbiology 2020Quote: ... and plasmids containing the full-length reverse transcriptase gene were isolated with the QIAprep Spin Miniprep kit (Qiagen, Valencia, CA). A minimum of 20 clones from each sample were sequenced ...