Labshake search
Citations for Qiagen :
651 - 700 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: GAS muscles were pulverized on a metal block cooled with liquid nitrogen before lysis and RNA extraction using the RNeasy Fibrous Tissue Mini Kit (74704, Qiagen). RNA purity and integrity was examined with a Bioanalyser (Agilent).
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bioRxiv - Physiology 2024Quote: ... The reaction was run in the QIAcuity Nanoplate 26k 24-well plates (QIAGEN). The 40 µl reaction mix consisted of 10 µl of 4x QIAcuity Probe PCR Kit (QIAGEN) ...
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bioRxiv - Plant Biology 2024Quote: ... Sequencing reads were processed by the CLC Genomics Workbench 9.5.1 software (Qiagen). Reads were mapped to the Aethionema arabicum PacBio contigs originating from the CYP accession 13 ...
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bioRxiv - Physiology 2024Quote: ... lysed in 300µL of RLT buffer (Qiagen) and stored at -80°C until extraction of all samples ...
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bioRxiv - Physiology 2024Quote: ... DNeasy Blood & Tissue Kits were used (QIAGEN #69506). After extraction ...
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bioRxiv - Physiology 2024Quote: ... DNA purification with mini elute kit (Cat# 28004, QIAGEN, Hilden, Germany) following quantification using Qubit ds DNA quantification assay kit (Invitrogen #Q32851 ...
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bioRxiv - Physiology 2024Quote: ... and RNeasy (Qiagen) hybrid protocol ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA was extracted from pennycress or Arabidopsis seedlings using the RNeasy Plant Mini kit (Qiagen) with in-column DNase treatment ...
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bioRxiv - Physiology 2024Quote: ... Reverse transcription of RNA into cDNA was performed by M-MLV reverse transcriptase and oligo(dT) primers (Qiagen, Toronto, ON, Canada). cDNA was then amplified using aCFX384 Touch Real-Time PCR Detection Systems (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: Gephyrin P1 and cysteine variants in pQE80L (Qiagen) were cloned by Gibson assembly using EcoRI into pAAV_hSyn_mScarlet (a gift from Karl Deisseroth ...
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bioRxiv - Molecular Biology 2024Quote: ... and extracted with Jetgene mini prep kit (QIAprep Spin Miniprep Kit, QIAGEN). The correctness of constructions was verified by sequencing (Eurofins) ...
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bioRxiv - Neuroscience 2024Quote: ... RNA was isolated with the RNeasy mini kit (Qiagen). 1000 ng of RNA from each sample was transcribed to cDNA with FIREScript reverse transcriptase (Solis Biodyne ...
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bioRxiv - Neuroscience 2024Quote: ... mRNA was isolated using RNeasy Mini Kit (Qiagen) and cDNA was generated using the ProtoScript II Reverse Transcription Kit (New England BioLabs) ...
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bioRxiv - Neuroscience 2024Quote: ... RNA and DNA were extracted from each SCN sample using the AllPrep DNA/RNA Micro Kit (Qiagen) according to the instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Bacterial DNA was extracted using the QIAamp Powerfecal DNA kit (Qiagen), and its concentration was quantified by Nanodrop 2000 C Spectrophotometer (ThermoFisher Scientific).
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bioRxiv - Neuroscience 2024Quote: RNA was purified using the PureLink RNA mini kit with in-column DNAse-I digest (#79254, Qiagen) following the manufacturer’s instructions (#12183018A ...
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bioRxiv - Neuroscience 2024Quote: ... The total RNA extraction was carried out using the RNeasy Lipid Tissue Mini Kit (Qiagen. The Netherlands) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: The samples were moved into QIAzol lysis reagent (Qiagen. The Netherlands) and homogenized using a Tissue-Tearor (BioSpec Products ...
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bioRxiv - Neuroscience 2024Quote: ... Total RNA was isolated using the RNeasy Plus mini kit (QIAGEN #74134) according to the manufacturer’s instructions and processed for sequencing at the Bioinformatics and Expression Analysis core facility at Karolinska Institutet ...
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bioRxiv - Neuroscience 2024Quote: ... All samples were treated with RNase Free DNase Set (Qiagen. The Netherlands) to remove genomic DNA contamination ...
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bioRxiv - Neuroscience 2024Quote: ... and purified using the miRNeasy Mini Kit (Qiagen; 217004). A 5’-adenylated DNA adapter (5’-rAppAGATCGGAAGAGCACACGTCT-NH2-3’ ...
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bioRxiv - Neuroscience 2024Quote: ... followed by DNase treatment (Qiagen). RNA was quantified using the NanoDrop 2000c instrument ...
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bioRxiv - Neuroscience 2024Quote: DNA was extracted from organs using QIAamp DNA FFPE Tissue Kit (Qiagen, cat no 56404) and from blood samples using DNeasy Blood and Tissue Kit (Qiagen ...
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bioRxiv - Neuroscience 2024Quote: ... and from blood samples using DNeasy Blood and Tissue Kit (Qiagen, cat no 69504) according to manufacturer’s protocol except for the final elution step being done with a low TE buffer instead of the elution buffer provided in the kit ...
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bioRxiv - Neuroscience 2024Quote: ... RNA was isolated from washed FAC-sorted cells using an AllPrep DNA/RNA Micro Kit (Qiagen). RNA was extracted from cortical tissues from three biological replicates for each age using TRIzol (Invitrogen) ...
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bioRxiv - Microbiology 2024Quote: DNA was extracted in random order from the faecal slurries (n=484) using DNeasy PowerLyzer PowerSoil kit (Qiagen, 12855-100) and the V3-region of the 16S rRNA gene was PCR amplified using 0.2 µl Phusion High-Fidelity DNA polymerase (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... viral RNA was extracted from challenge materials and BALF samples using a QIAamp Viral RNA mini kit (Qiagen, Germantown, MD, USA) and subjected to multi-segment reverse transcription polymerase chain reaction (RT-PCR ...
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bioRxiv - Microbiology 2024Quote: DNA was extracted from 250 mg of dry weight soil using the DNeasy PowerSoil DNA extraction Kit (Qiagen, FR) following the kit’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... RNA was isolated from the pelleted samples with a miRNeasy micro kit (Qiagen) following the manufacturer’s instructions and stored at −80°C for further analysis ...
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bioRxiv - Microbiology 2024Quote: ... Reads were then demultiplexed and parsed into individual FASTQ files that were imported into CLC Genomics Workbench version 22.0.1 (Qiagen, Germantown, MD, USA) for analysis ...
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bioRxiv - Microbiology 2024Quote: ... The PCR sample was then digested with DpnI to cut the residual template DNA and purified with the Pure Link PCR purification kit (Qiagen, Hilden, Germany). The DNA fragment was labeled with 5-(3-aminoallyl)dUTP by nick translation according to the manufacturer’s protocol (Ares DNA labeling kit ...
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bioRxiv - Microbiology 2024Quote: RNA was extracted using an RNeasy PowerMicrobiome Kit (QIAGEN, 26000-50) using the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Total RNA was extracted using Qiagen RNAeasy Mini kit (Qiagen, Germany) following the manufacturer’s protocol with a few modifications described previously [22] ...
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bioRxiv - Cell Biology 2024Quote: ... 4 μl of siRNAs were mixed with 37.5 μl of Hiperfect transfection reagent (301707, Qiagen) and enough Optimem Opti-MEM reduced serum medium (31985070 ...
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bioRxiv - Microbiology 2024Quote: ... and a de novo assembly was performed using CLC genomics workbench 20.0.4 (QIAGEN, Hilden, Germany). Finally ...
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bioRxiv - Molecular Biology 2024Quote: ... miRCURY LNA SYBR Green kit (cat# 339346) was purchased from Qiagen (Hilden, Germany). iTaq Universal SYBR Green One step RT-PCR kit (cat# 1725150 ...
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bioRxiv - Microbiology 2024Quote: ... IFAS and LifeGuard Soil Preservation were transferred into a 5 mL bead beating tube from DNeasy PowerWater kits for 5-minutes of vortexing (Qiagen, Hilden, Germany). Afterwards ...
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bioRxiv - Microbiology 2024Quote: ... and mapping of the reads on a reference (MT594401.1) using CLC genomics workbench software v.21.0.5 (Qiagen). Parameters for reference-based assembly consisted of match score = 1 ...
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bioRxiv - Microbiology 2024Quote: ... Excess rRNA was depleted using QiaSeq FastSelect 5S/16S/23S depletion kit as well as the FastSelect HMR and Plant rRNA probes (Qiagen, Hilden, Germany). RNA sequencing was performed on an Illumina NovaSeq S1 PE100bp to obtain 1.6 billion reads (Illumina Inc ...
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bioRxiv - Microbiology 2024Quote: ... 2020b).Nucleic acid from 100µL of cell supernatant were extracted using QIAamp 96 DNA kit and Qiacube HT robot (both from Qiagen). Viral RNA was quantified by real-time RT-qPCR (GoTaq 1 step RT-qPCR kit ...
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bioRxiv - Microbiology 2024Quote: ... followed by Proteinase K (Qiagen, final concentration 240 ng/µl) at 55°C for 1 hr ...
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bioRxiv - Microbiology 2024Quote: ... All samples were subjected to a 1-hour DNAse treatment (Qiagen RNase-free DNase kit), and final elution volume was 20 μl ...
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bioRxiv - Microbiology 2024Quote: ... and feces was performed by the UW Biotechnology Center following the protocol for the DNAeasy 96 PowerSoil Pro QIAcube HT Kit (Qiagen, Hilden, Germany). Bacterial 16S rRNA gene amplicon libraries targeting the V3-V4 region or the fungal nuclear ribosomal internal transcribed spacer (ITS ...
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bioRxiv - Microbiology 2024Quote: ... the 300-500 bp region was excised and DNA was extracted using the MinElute Gel Extraction Kit (Qiagen).
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bioRxiv - Developmental Biology 2024Quote: ... Tissue punches were homogenized using the TissueLyser II (Qiagen), and aliquoted into separate tubes for DNA and RNA extractions ...
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bioRxiv - Microbiology 2024Quote: ... 2 g of soil were used for RNA extraction using the RNeasy PowerSoil Kit (Qiagen, Venlo, Netherlands) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... we used the DNeasy PowerSoil Pro kit (QIAGEN, 47016) and followed the manufacturer’s standard protocol included in the kit using 100μl of the bacterial culture ...
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bioRxiv - Microbiology 2024Quote: ... The PCR products were then mixed at equal density ratios and purified with a Gel extraction kit (QIAGEN, Düsseldorf, Germany). Sequencing libraries were generated with the NEBnext® UltraTM DNA Library Prep kit for Illumina® (New England Biolabs ...
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bioRxiv - Microbiology 2024Quote: ... was extracted with DNeasy PowerLyzer Microbial Kit (QIAGEN) to assess if the contamination was present in the original samples ...
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bioRxiv - Microbiology 2024Quote: ... 150 μl of the supernatant was extracted with a DNeasy blood & tissue kit (Qiagen, Maryland, USA), and were extracted as purified DNA in 50 μl of distilled water ...