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Citations for Qiagen :
901 - 950 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The PCR sample was then digested with DpnI to cut the residual template DNA and purified with the Pure Link PCR purification kit (Qiagen, Hilden, Germany). The DNA fragment was labeled with 5-(3-aminoallyl)dUTP by nick translation ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were washed with PBS and harvested for RNA extraction using miRNeasy mini kit (QIAGEN, 217004) according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2024Quote: ... the solubilized His-tagged histones were purified using Ni-NTA beads (Qiagen). For untagged H2B ...
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bioRxiv - Molecular Biology 2024Quote: ... Islets for qPCR were stored in buffer RLT (RNeasy Micro Kit, QIAGEN, Cat # 74004) + 1% β-Mercaptoethanol (Sigma Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... All DNA extraction was conducted using the DNeasy Plant Pro kits (Qiagen Canada, Toronto, ON) with a Qiacube (Qiagen Canada) ...
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bioRxiv - Molecular Biology 2024Quote: RNA was extracted from mouse islets using the RNAeasy Micro Kit (QIAGEN, Cat # 74004); RNA was extracted from flash-frozen liver and peripancreatic fat tissues using TRIzolTM (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... along with a pre-chilled 5-mm steel bead (QIAGEN). All samples were then placed in pre-chilled cassettes for the Tissue Lyser II and pulverized for at 30 1/s for three 1-minute oscillations ...
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bioRxiv - Molecular Biology 2024Quote: ... and 10 μl of Quantifast SYBR Green PCR Master Mix (QIAGEN) or PowerUp™ SYBR™ Green Master Mix (ThermoFisher ...
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Assessment of salivary microRNA by RT-qPCR: Challenges in data interpretation for clinical diagnosisbioRxiv - Molecular Biology 2024Quote: Q-PCR reactions using miRCURY LNA SYBR Green PCR Kit and miRCURY LNA miRNA PCR Assay (Qiagen) were prepared in 384 multi-well plates ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA isolation from 200 mg of grounded tissue was performed with the RNeasy plant mini kit (Qiagen, Hilden, Germany) and the cDNA synthesis with the First Strand cDNA Synthesis Kit (Thermo Scientific ...
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Assessment of salivary microRNA by RT-qPCR: Challenges in data interpretation for clinical diagnosisbioRxiv - Molecular Biology 2024Quote: Reverse transcriptase reactions using miRCURY LNA RT Kit (Qiagen) for synthetic and/or purified salivary miRNA were prepared in 96 well plates ...
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bioRxiv - Cancer Biology 2024Quote: ... Viable (PI-) single cells were sorted into 2.5µL of RLT PLUS buffer (Qiagen) containing 2.5U SUPERas-In (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by miRNeasy mini column (Qiagen). cDNA was generated from 1-2 mg of total RNA using random hexamers and Maxima Reverse Transcriptase (Thermo Fisher Scientific) ...
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Assessment of salivary microRNA by RT-qPCR: Challenges in data interpretation for clinical diagnosisbioRxiv - Molecular Biology 2024Quote: ... All salivary miRNA were extracted using miRNeasy Serum/Plasma Kit (Qiagen) according to the instruction of the manufacturer except for the elution step in which extracted salivary small RNAs were collected in 20 μL nuclease free water ...
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bioRxiv - Molecular Biology 2024Quote: ... using the QuantiFast SYBR Green PCR Kit (Qiagen). Thermocycling was done for 40 cycles in a two-step cycling in accordance with the manufacturer’s instructions and each PCR reaction was performed in triplicate ...
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bioRxiv - Molecular Biology 2024Quote: ... and the DNA was purified using a QIAfilter Plasmid Midi Kit (Qiagen, 12243). Biotin-containing handles were made using a PCR on pBluescript SK+ (Stratagene ...
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bioRxiv - Microbiology 2024Quote: ... with a Qiacube (Qiagen Canada). The extracted DNA was dissolved in 50 µ L water ...
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bioRxiv - Molecular Biology 2024Quote: ... and Total RNA was isolated using miRNeasy mini kit (QIAGEN, Cat# 217004) according to the manufacturer’s protocol and as previously described22,65 ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were lysed with QIAzol lysis reagent (QIAGEN, Cat# 79306), and Total RNA was isolated using miRNeasy mini kit (QIAGEN ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was isolated from 10µm-thick sections of FFPE material using the RNeasy FFPE kit according to manufacturer’s instructions (Qiagen, 73504). RNA concentration was determined using a Nanodrop spectrophotometer (ThermoScientific ...
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bioRxiv - Microbiology 2024Quote: The viral RNA was extracted using a mini viral RNA kit (Qiagen Sciences) in an automated QIAcube platform according to the manufacturer’s instructions (20) ...
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bioRxiv - Microbiology 2024Quote: ... The pellets were resuspended with OMV buffer and re-pelleted again by centrifugation at 100,000 g (31,000 RPM) for 2 h at 4°C and lysed with Qiazol followed by RNA isolation with the miRNeasy kit (Qiagen, Germantown, MD) to obtain total RNA including the small RNA fraction ...
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bioRxiv - Microbiology 2024Quote: ... run using the QIAcuity One Digital PCR System (Qiagen, 2-plex Device, Cat. No. 911001). The following conditions were used for the one-step cycling dPCR program ...
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bioRxiv - Microbiology 2024Quote: ... Amplification and detection were performed using the QIAcuityTM OneStep Advanced Probe Kit (Qiagen, Cat. No. 250132), with primers and probe targeting the influenza A virus M gene segment (specific amplicon length is 110 bp) ...
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bioRxiv - Microbiology 2024Quote: ... followed by purification and DNase treatment (RNeasy kit, RNase-Free DNase Set, Qiagen). RNA was submitted for 150 bp paired-end sequencing on an Illumina HiSeq platform (Azenta Life Sciences) ...
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bioRxiv - Microbiology 2024Quote: ... the reactions were loaded into QIAcuityTM Nanoplate 8.5k 96-well (Qiagen, Cat. No. 250021) run using the QIAcuity One Digital PCR System (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... mosquitoes were homogenized in 500 μl ice-cold 1X Phosphate Buffered Saline buffer with two ice-cold steel bearing balls (3 mm diameter, LOUDET) using a TissueLyser II (Qiagen) and clarified through centrifugation ...
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bioRxiv - Microbiology 2024Quote: ... and genomic DNA extracted using the DNeasy Kit (Qiagen, Hilden, Germany). Genome was sequenced using a Hi Seq 2000 (Illumina Inc. ...
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bioRxiv - Microbiology 2024Quote: RNA extraction was performed using the QIAamp Viral RNA Mini extraction kit (Qiagen, Cat. No. 52906) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... using the QIAamp DNA Mini Kit (Qiagen, Germantown, MD) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... reverse transcription quantitative PCR (RT-qPCR) assays were performed with the QuantiTect Virus + ROX Vial Kit (Qiagen, Toronto, ON, Canada) in a LightCycler® 480 system (Roche Molecular System) ...
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bioRxiv - Cell Biology 2024Quote: ... DNase (Qiagen) treatment was performed to remove residual DNA ...
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bioRxiv - Microbiology 2024Quote: ... and a de novo assembly was performed with CLC genomics workbench 20.0.4 (QIAGEN, Hilden, Germany). Finally ...
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bioRxiv - Cell Biology 2024Quote: ... and RNA was isolated at day 7 using RNeasy Micro Kit (Qiagen). For each experiment untreated vehicle controls were applied ...
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bioRxiv - Cell Biology 2024Quote: ... instrument and RNA was isolated from sorted cells using RNeasy Micro Kit (Qiagen). Seeding efficiency was quantified by counting the number of structures forming after seeding as single cells.
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bioRxiv - Molecular Biology 2024Quote: ... and RNeasy kit (Qiagen, Germany) protocols ...
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bioRxiv - Microbiology 2024Quote: ... or the EZ2 Connect system (EZ1&2 Virus Mini Kit v2.0, Qiagen, Toronto, ON, Canada). Then ...
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bioRxiv - Microbiology 2024Quote: ... or the EZ2 Connect system (EZ1&2 Virus Mini Kit v2.0, Qiagen). Then ...
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bioRxiv - Microbiology 2024Quote: ... following the manufacturer’s protocol for the QIAamp MinElute Virus Spin Kit (Cat. 57704, Qiagen, Hilden, Alemania). The resulting DNA for each sample was quantified with a Qubit fluorometer (Cat ...
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bioRxiv - Cell Biology 2024Quote: The RNA was extracted from the sorted HSCs using the Trizol-chloroform method followed by DNase treatment in the solution and cleaned up with an RNeasy MinElute clean-up kit from Qiagen. The purity of RNA was confirmed on the Bioanalyzer before proceeding with the sequencing ...
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bioRxiv - Cell Biology 2024Quote: Ingenuity pathway analysis (IPA) software (QIAGEN Inc. ...
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bioRxiv - Microbiology 2024Quote: ... Total RNA was extracted from lysed cells using the RNeasy ® Mini kit (Qiagen) and DNA was removed using Turbo DNA-free™ Kit (Invitrogen) ...
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bioRxiv - Microbiology 2024Quote: ... The total RNA from frozen cells was extracted by using RNeasy-Mini kit (QIAGEN), followed by treatment with DNase I (TURBO DNA-free™ Kit) ...
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bioRxiv - Microbiology 2024Quote: ... was resuspended in 200 µL of PBS and DNA was isolated using the gram-positive protocol of the Dneasy Blood and tissue Kit (Qiagen). For each isolation ...
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bioRxiv - Microbiology 2024Quote: ... Power Biofilm DNA Isolation kits (Qiagen) were used for DNA isolation and DNA samples were stored in -80°C freezer ...
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bioRxiv - Microbiology 2024Quote: All DNA isolations were performed using the Dneasy Blood and tissue Kit (Qiagen). Each culture was centrifuged at 1500 xg for 10 min at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... The pellet from the first centrifugation was resuspended in 200 µL of PBS and DNA was isolated using the cultured cells protocol of the Dneasy Blood and tissue Kit (Qiagen). The second pellet ...
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bioRxiv - Cell Biology 2024Quote: RNA was isolated from RPE-1 cells using the RNeasy mini kit (Qiagen, CA) and cDNA was generated using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Microbiology 2024Quote: ... and extracted twice in stool lysis buffer ASL (Qiagen, Hilden Germany). Bead beating at a speed of 5 m/s for 60 seconds (x2 ...
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bioRxiv - Microbiology 2024Quote: ... and purified using QIAamp DNA Mini Kit (Qiagen). The purified DNA was tested for purity with a nanophotometer (Implen NP80 ...