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Citations for Qiagen :
901 - 950 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... DNA of each isolate was extracted with a commercial kit according to manufacturer instructions (Qiagen) and kept at -20°C before amplification ...
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bioRxiv - Microbiology 2024Quote: ... DNA was extracted using the DNeasy Blood and Tissue Kit (Qiagen GmbH, Hilden, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: DNA was extracted from 250mg edm for the 246 samples (compositional variants and original soils) using the DNeasy PowerSoil-htp 96 well DNA isolation kit (Qiagen, Hilden, Germany). Generation of amplicons for Illumina MiSeq sequencing was done according to a two steps PCR protocol as follows ...
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bioRxiv - Microbiology 2024Quote: ... before proceeding according to the protocol and eluting in 100 µL of elution buffer (Qiagen). DNA yields were quantified via fluorometry (Qubit 2.0 ...
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bioRxiv - Microbiology 2024Quote: ... Products were then washed multiple times with 80% ethanol and the dried pellet was resuspended in 32.5 µL EB buffer (Qiagen), incubated for two minutes at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... except that samples were homogenized in bead tubes using a TissueLyser II (Qiagen) for ten minutes at 30/sec ...
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bioRxiv - Microbiology 2024Quote: ... one fecal pellet per procedure was placed in a 2 mL round-bottom tube containing a 0.5 cm diameter stainless steel bead and 500 µL of sterile phosphate-buffered saline (PBS) and agitated at 1/30s using a TissueLyser II (Qiagen) for 60 seconds ...
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bioRxiv - Microbiology 2024Quote: Fecal DNA was extracted using PowerFecal kits (Qiagen) according to the manufacturer instructions ...
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bioRxiv - Microbiology 2024Quote: Total DNA was extracted from gut feces samples collected using a QIAamp Fast DNA Stool Mini Kit following the manufacturer’s recommendations (QIAGEN GmbH, Germany). Then ...
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bioRxiv - Microbiology 2024Quote: ... Total RNA was extracted with the RNeasy kit (Qiagen, Hilden, Germany). Additionally ...
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bioRxiv - Immunology 2024Quote: ... The PELO protein was purified with Ni2+-NTA-agarose (Qiagen) and followed by further purification by Superdex 200 10/30 prepacked column (GE Healthcare) ...
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bioRxiv - Molecular Biology 2024Quote: ... All samples used for Southern blotting and qPCR were treated with RNase (Qiagen). For Southern blot analysis total DNA (3-10 μg ...
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bioRxiv - Molecular Biology 2024Quote: ... 2.0 (Qiagen). A single PCR reaction was employed to amplify a 192 bp fragment spanning the c.420G>T mutation site ...
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bioRxiv - Molecular Biology 2024Quote: Quantification of Polrmt c420G>T was performed on tail DNA using a PyroMark-Q24 pyrosequencer (Qiagen). Allele Quantification assay was developed using PyroMark assay design software v ...
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bioRxiv - Molecular Biology 2024Quote: ... For Southern blotting or qPCR total DNA was isolated from mouse tissues using the DNeasy Blood & Tissue Kit (QIAGEN) as previously described(Kühl et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were purified and denatured using a PyroMark Q24 vacuum workstation (Qiagen). Sequencing was performed with PyroMark Gold Q24 reagents according to manufacturer’s instructions using the sequencing primer (CAAGATCTGGAACAAGAA) ...
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bioRxiv - Molecular Biology 2024Quote: ... washed with PBS using a 96-well plate manifold base (Qiagen) connected to the vacuum and eluted into 96-well PCR plates using 86 μl of 0.1 M glycine-HCl buffer ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was isolated from the glia cells using the RNeasy Kit (Qiagen, Milano, Italy) following the manufacturer’s instructions for cells treated with 100 nM or 5μM SAHA in the absence or presence of 10 ng/ml LPS after 4 hours.
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bioRxiv - Molecular Biology 2024Quote: ... Ni-NTA beads (Qiagen) were mixed with lysate supernatant for 30 min and washed with buffer A and eluted with buffer C (25 mM HEPES pH=7.5 ...
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bioRxiv - Neuroscience 2024Quote: RNA from immunopanned astrocytes or neurons from 10-month hCOs was isolated using RNAeasy Micro Kit (Qiagen, 74004). Bulk RNA sequencing libraries were prepared using the NEBNext Ultra II kit with PolyA Selection and sequenced using the Illumina HiSeq 4000 System to a minimum of 30 million paired-end reads per sample ...
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bioRxiv - Immunology 2024Quote: ... RNA was extracted for sequencing using RNeasy Mini Kit (Qiagen) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2024Quote: ... the samples were processed using the DNeasy Plant Mini Kit (Qiagen), and 1 µl of DNA was used as a template ...
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bioRxiv - Microbiology 2024Quote: ... The amplification was performed in a 25 μl reaction volume containing 12.5 μl of Rotor-Gene Multiplex PCR Master Mix (Qiagen), 2 μl of the primer mix (5 µM each primer concentration ...
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bioRxiv - Microbiology 2024Quote: ... The DNA was further isolated from the bacteria using DNeasy Blood and Tissue Kit (Qiagen), and the identity was further confirmed by sequencing dnaA (Dobhal et al ...
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bioRxiv - Microbiology 2024Quote: Total RNAs were extracted from 100 mg of plant tissue using RNeasy Plant Mini Kit (Qiagen). One μg of total RNA was used for northern blot analysis using two ToANV probes (+ and – strand ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.5mL Ni-NTA Agarose slurry (QIAGEN) for each 50mL of supernatant were equilibrated in 1X Binding buffer (50 mM Sodium Phosphate buffer pH 8.0 at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... peregrina and ground under liquid nitrogen to extract genomic DNA using Qiagen Genomic-Tip (Qiagen, Hilden, Germany). A short-read sequence library was prepared using the PCR-free Swift 2S Turbo Flexible DNA Library Kit (Swift Sciences ...
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bioRxiv - Molecular Biology 2024Quote: ... Ni-NTA Agarose (Qiagen) was added to the clarified lysate for 30 minutes and moved to stationary column ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.5mL Ni-NTA Agarose slurry (QIAGEN) each 50mL of supernatant were equilibrated in 1X Binding buffer (20 mM Tris ...
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bioRxiv - Molecular Biology 2024Quote: RNA was isolated from crude heart mitochondria using the miRNeasy mini kit (QIAGEN) and the concentration ...
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bioRxiv - Molecular Biology 2024Quote: ... PyroMark binding buffer (Qiagen), and 1 μL Streptavidin sepharose TM high performance beads (GE Healthcare) ...
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bioRxiv - Molecular Biology 2024Quote: ... Relative allele frequencies were calculated using the PyroMark-Q24 Advance v.3.0.0 sfortare (Qiagen).
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bioRxiv - Molecular Biology 2024Quote: RNA was isolated either by using the miRNeasy mini kit (QIAGEN). Reverse transcription PCR (RT-PCR ...
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bioRxiv - Molecular Biology 2024Quote: ... About 20 μg of grinded tissue were used to extract DNA using the blood and tissue kit or the Gentra Puregene tissue kit (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... MtDNA was isolated by phenol/chloroform extractions or by Puregene Core Kit A (QIAGEN) and radiolabeled replicated DNA was analyzed by Southern/ D-loop Southern blotting (described earlier ...
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bioRxiv - Molecular Biology 2024Quote: ... A mouse Yy1-specific siRNA or the AllStars Negative Control siRNA (Qiagen) was co-transfected with an L1 promoter reporter plasmid using Lipofectamine 3000 (Invitrogen) ...
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bioRxiv - Molecular Biology 2024Quote: ... Double‐stranded cDNA was purified on QIAquick columns (Qiagen) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: 20 μL EB (10 mM TrisCl, pH 8.5, Qiagen) were added to the reaction ...
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bioRxiv - Molecular Biology 2024Quote: ... RNAs were extracted using RNeasy Mini Kit (Qiagen) and infection confirmed using RT-qPCR quantification of viral RNAs using specific primers pairs ...
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bioRxiv - Molecular Biology 2024Quote: ... and total cellular RNA was purified using RNeasy Mini Kit (Qiagen) and RNase-free DNase Set (Qiagen) ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA was extracted using the RNeasy Mini Kit (Qiagen) and infection confirmed plaque assay and RT-qPCR using primers for the viral N gene ...
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bioRxiv - Molecular Biology 2024Quote: ... and RNase-free DNase Set (Qiagen). Infection was confirmed by RT-qPCR using specific primers to detect HDV transcripts.
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bioRxiv - Molecular Biology 2024Quote: ... for frozen tissues and using the Qiagen RNAeasy kit (Qiagen 73504) for FFPE specimens ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was extracted using the Qiagen RNAeasy Mini Kit (Qiagen, 74106), for frozen tissues and using the Qiagen RNAeasy kit (Qiagen 73504 ...
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bioRxiv - Microbiology 2024Quote: ... Genomic DNA was extracted using the QIAmp DNA Mini Kit (Qiagen). Reverse transcript products (gag or second strand transfer ...
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bioRxiv - Molecular Biology 2024Quote: ... rRNA depletion was performed using the QIAseq FastSelect 5S/16S/23S kit (Qiagen, UK) in accordance with manufacturer’s instructions with the addition of RNase inhibitor (New England BioLabs ...
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bioRxiv - Molecular Biology 2024Quote: ... and RNA was purified with RNeasy mini kit (Qiagen, cat. 50974104). RNA concentration and integrity were checked by fluorometry (Qubit RNA HS ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were removed from the plate with 250µL of RNAprotect Cell Reagent (Qiagen 76104) and stored at -20°C for at least 24 hours prior to extraction ...
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bioRxiv - Molecular Biology 2024Quote: ... were lysed using QIAshredder (Qiagen 79656). Total RNA was then extracted using the RNeasy Mini Kit (Qiagen 74106 ...
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bioRxiv - Molecular Biology 2024Quote: ... beads were resuspended in 400 µL of RLT buffer (Qiagen, cat. 79216s) and RNA was purified with RNeasy mini kit (Qiagen ...