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Citations for Qiagen :
851 - 900 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... the samples were purified with 500 µL of PB buffer and 650 µL of PE buffer and eluted in 30 µL of EB buffer (MinElute PCR Purification Kit, QIAGEN). As previously ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA was extracted from human tissue and cells using the miRNeasy Mini kit (Qiagen, Hilden, Germany) or TRIzol reagent (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2024Quote: ... Amplifications were carried out on a RotorGene (Qiagen) thermalcycler following the next program ...
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bioRxiv - Molecular Biology 2024Quote: ... genomic DNAs were purified by using the DNeasy Blood & Tissue Kit (Qiagen). The DNA was then fragmented to 100-600 bp by sonication ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were then transferred to a RNeasy spin column (Qiagen, 74134) in 700 µL aliquots and spun at 12,000 x g for 15 minutes ...
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bioRxiv - Genetics 2024Quote: ... total RNA was isolated with the RNeasy Mini Kit (Qiagen # 74136) from B16F10 WT and Capza3 KO cells ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was isolated from approximately 10–20 seedlings using the RNeasy Plant Mini Kit (QIAGEN) followed by DNase I treatment ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was isolated using the RNeasy mini kit (Qiagen, Germantown, MD, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Using miRCURY LNA RT kit (Qiagen), we first generated cDNA ...
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bioRxiv - Microbiology 2024Quote: ... RNA samples were initially preserved with 1 mL of activated sludge (i.e., suspended phase) in 9 mL of LifeGuard Soil Preservation (Qiagen, Hilden Germany). This solution was thawed and centrifuged (16,000 g for 1-minute ...
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bioRxiv - Microbiology 2024Quote: ... while samples for RNA-based analyses were mixed at a 1:10 ratio of LifeGuard Soil Preservation (Qiagen, Hilden Germany) before being put on dry ice ...
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bioRxiv - Molecular Biology 2024Quote: We isolated total RNA from thio-MPMs and BMDMs using miRNeasy kit (Qiagen) following manufacturers protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Validated primers were obtained from Qiagen, UK for the miRNAs ...
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bioRxiv - Developmental Biology 2024Quote: ... using Attractene (301005; Qiagen) in Opti-MEM I (51985-034 ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA (1 μg) was treated with DNaseI (Qiagen). cDNA was generated using M-MLV Reverse Transcriptase (Invitrogen) ...
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bioRxiv - Molecular Biology 2024Quote: ... Qiagen Taq polymerase (Qiagen) was used for PCR amplification of target transcripts and products were resolved and imaged on 1-2% agarose gels (Chemi-Doc) ...
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bioRxiv - Microbiology 2024Quote: ... Total RNA was extracted using the RNeasy Mini Kit (Qiagen), according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... 2015] and purified with RNeasy Mini Kit (Qiagen, Hilden, Germany). Polyadenylated RNA fragments (150 – 500 nt ...
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bioRxiv - Genomics 2024Quote: ... 500 µl liquid culture were mixed well with 1 ml RNAprotect Bacteria Reagent (Qiagen) and incubated for 5 min ...
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bioRxiv - Molecular Biology 2024Quote: ... the supernatant was added to a gDNA eliminator column (Qiagen RNeasy plus kit, 74034) and spun at RT for 30 seconds at 10,000RPM ...
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bioRxiv - Genomics 2024Quote: ... Total RNA extraction was performed using the RNeasy Plant Mini Kit (Qiagen, Hilden, Germany). A cDNA library was built following the instructions ...
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bioRxiv - Genomics 2024Quote: ... Real-time PCR was conducted with cDNA as the template using the Quantitect SYBR Green PCR kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: DNA was extracted from the whole bodies of pooled individuals of five adult males and five adult females of each psyllid species using the DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany). The quality of the extracted DNA was assessed using a NanoDrop 2000c spectrophotometer (Thermo Fisher Scientific) ...
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Srs2 binding to PCNA and its sumoylation contribute to RPA antagonism during the DNA damage responsebioRxiv - Molecular Biology 2024Quote: ... The cleared supernatant was obtained after centrifugation for 20 min and was then incubated with Ni-NTA resin (Qiagen 30210) in the presence of 0.05% Tween-20 and 4.4 mM imidazole overnight at room temperature with rotation ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was then extracted using the RNeasy® Micro Kit (Qiagen) with the optional DNase digest step ...
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bioRxiv - Molecular Biology 2024Quote: ... All PCR products were purified using a QIAquick PCR Purification Kit (Qiagen) and subjected to dye-terminator cycle sequencing using DTCS Quick Start Mix (Beckman Coulter ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA was extracted from the pelleted cells using the QIAgen DNeasy Blood and Tissue Kit protocol and QIAgen Qiacube (QIAGEN, Germany). The DNA concentration of extracts were measured ...
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bioRxiv - Molecular Biology 2024Quote: ... We typically dissected about 20 slices per HVC and transferred the tissue into an Eppendorf tube containing 340 μl of RLT buffer mixture (with DTT, Qiagen, Valencia, California). We used these rather thin sections for dissection of HVC to avoid contamination of the samples with non-HVC tissues ...
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bioRxiv - Molecular Biology 2024Quote: ... we sequenced the rbcL F3R3 region of 104 predominant plant species at the study site using the following methods. Total DNA was extracted from plant specimens (ca. 0.8 cm2) using a DNeasy Plant Mini Kit (Qiagen) and purified using a Geneclean Spin Kit (MP-Biomedicals) ...
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bioRxiv - Neuroscience 2024Quote: ... Plasmids were purified by Qiagen Plasmid Mega kit and then sent to Viral Core Facility of Universtatmedizin Berlin Charite to be packaged into AAV 2/1 ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by purification of the RNA transcripts using the RNeasy Kit (Qiagen) as per the supplied protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... 4 µl of 5X PCR buffer (Qiagen; Hilden, Germany), 0.8 µl of 10 mg/ml BSA (Promega ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 µl Qiagen CL buffer (10x; Qiagen, Hilden, Germany), 0.4 µl MgCl2 (25 mM ...
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bioRxiv - Evolutionary Biology 2024Quote: ... alphenor females that were homozygous for the h or H alleles using the QIAgen GenomicTip G-100 kit (QIAgen, USA). Extractions followed the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: The RNA was further purified using the RNeasy MinElute Cleanup kit (Qiagen) following the manufacturer’s recommendations with minor modifications ...
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bioRxiv - Evolutionary Biology 2024Quote: ... or spleen with the DNeasy Blood and Tissue kit (Qiagen, Germany) following the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2024Quote: RNAseq was performed on RNA extracted and DNAse treated samples using the miRNeasy mini kit (Qiagen) obtained from three replicates of miRNA overexpression ...
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bioRxiv - Developmental Biology 2024Quote: Total RNA was extracted from fetal gonads (with mesonephros removed) using a RNeasy Mini kit (74104, Qiagen) or a RNeasy Micro kit (74004 ...
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bioRxiv - Microbiology 2024Quote: Viral RNA was extracted from 100 μL of cell supernatant from passages P8 and P16 using a QIAamp Viral RNA kit on the automated QIAcube (Qiagen), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... pyogenes using the DNeasy Blood and Tissue Kit (Qiagen 69504) with the following modifications ...
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bioRxiv - Microbiology 2024Quote: ... DNA was purified using the QIAquick PCR Purification Kit (Qiagen) per manufacturer’s instructions and eluted in 50 µl TE (0.1 mM EDTA).
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bioRxiv - Microbiology 2024Quote: 200µL of lung of infectious cell supernatant (virus stock and HAE) was inactivated with an equal volume of VXL lysis buffer (Qiagen) and viral RNA was extracted using an EZ1 Advanced XL robot with the EZ1 mini virus 2.0 kit (both from Qiagen ...
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bioRxiv - Microbiology 2024Quote: Right apical lobes were collected into 2mL tubes containing 0.75mL of Qiazol lysis reagent (Qiagen) and 3mm glass beads ...
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bioRxiv - Developmental Biology 2024Quote: ... sorted thymic epithelial cells from E16.5 embryos were either directly lysed in RLT buffer (QIAGEN, Catalog No. 74004) containing 40 mM DTT (ITW Reagents ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA was extracted using QIAGEN RNeasy Micro Kit (QIAGEN, Catalog No. 74004) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids were purified using the Spin MiniPrep kit (Qiagen 27104) per manufacturer’s instruction with the following modification for S ...
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bioRxiv - Microbiology 2024Quote: ... The PCR products were purified with the QIAquick PCR Purification Kit (Qiagen), pooled ...
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bioRxiv - Microbiology 2024Quote: ... and viral RNA was extracted using an EZ1 Advanced XL robot with the EZ1 mini virus 2.0 kit (both from Qiagen) and linear acrylamide (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... Inserts were PCR amplified using M13 primers and template plasmid was digested with DpnI before PCR purification (Qiagen). Digoxygenin labeled antisense probes were synthesized using SP6 or T7 RNA polymerase (Promega) ...
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bioRxiv - Developmental Biology 2024Quote: RNA extraction was performed with Dneasy Blood and Tissue Kit (Qiagen).