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Citations for abcam :
2401 - 2450 of 2500 citations for 6 Methyl 5 propyl 4 1H pyrimidinone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2020Quote: ... Immunostaining was performed by incubating different sections overnight at 4 °C using the following antibodies: human COMP (Abcam Cambridge, MA ab11056-rat 1:100), pS6 (1:200 2215S rabbit polyclonal Cell Signaling Technology) ...
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bioRxiv - Immunology 2020Quote: ... Membranes were blocked in 5% skim milk in TBS + 0.1% Tween 20 (TBS-T) for 1 hour at room temperature before incubation in primary antibody overnight at 4°C (rabbit-α-caspase-1, Abcam #ab179515, 1:1000). Membranes were washed in TBS-T ...
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bioRxiv - Neuroscience 2021Quote: ... The following primary antibodies were diluted in blocking buffer and applied to cells overnight at 4°C: chicken anti-βIII tubulin (Tuj-1, 1:200; Abcam #ab41489; Boston, MA, USA), rabbit anti-PSD95 (1:200 ...
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bioRxiv - Cell Biology 2021Quote: ... the fixed tissue sections were reacted individually overnight at 4°C with the following primary antibodies: rabbit anti-CD68 antibody (1:500, Abcam, Cat. #125212, Cambridge, MA), rabbit anti-CD146 antibody (1:500 ...
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bioRxiv - Genetics 2022Quote: ... for 1 hour at 4°C and then incubated overnight at 4°C with 1:1 mix of protein A/G Dynabead magnetic beads conjugated to H3K9me3 antibody (Abcam, cat# 8898 RRID: AB_306848).
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bioRxiv - Neuroscience 2024Quote: ... while the lower half was probed at 4 °C overnight with a 1:1000 dilution of a rabbit anti-β actin antibody (ab8227, Abcam, Waltham, MA, USA), in antibody dilution buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were then incubated for 16 h O/N at 4°C with the following primary antibodies: CASR (Abcam Cat# ab79038, RRID:AB_2071489, 1:500), MAP2 (Abcam Cat# ab92434 ...
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bioRxiv - Neuroscience 2023Quote: ... Following primary antibody incubation brains were washed 4 times for 15 minutes each in a 3% salt solution (in 1X PBST) and transferred to secondary antibodies (Abcam: ab175673; ThermoFisher: A-21236) in 1XPBST and incubated overnight at 4°C on a nutator ...
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bioRxiv - Cell Biology 2023Quote: ... Cells lysates were either mixed with 3 × Laemmli buffer for immunoblotting analysis or subject to pre-clearing and NDC80 complexes were isolated from 1 mg of cell lysate by 2-h incubation at 4°C with anti-NDC80 antibody cocktail (ProteinTech, 18932-1-AP; Abcam, ab3613; SantaCruz, sc-515550) or ...
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bioRxiv - Molecular Biology 2023Quote: ... in methanol and non-specific binding blocked with 10% serum from the source species for the secondary antibody followed by a primary antibody incubation overnight at 4 °C (Sox9, abcam, ab185966,; p16, (abcam, ab241543), α-SMA (Sigma-Aldrich’ A5691) ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then incubated overnight in primary antibody in the blocking solution at 4°C using 1 or more of the following antibodies: α-SMA antibody (ab7817, 1:500, Abcam, Cambridge, MA, USA), FAP antibody (ab53066 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were stained overnight at 4°C in blocking solution with the following antibodies and dilutions: Alexa Fluor® 488 anti-S100 beta (1:1000, Abcam, #ab196442, clone EP1576Y), PE anti-Nestin (1:150 ...
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bioRxiv - Bioengineering 2024Quote: ... Primary antibodies were added at 4 ºC overnight (Rabbit anti Lysozyme, Thermo Fisher Scientific PA129680, 1:50; Rabbit anti laminin, Abcam ab11575, 1:100). Samples were washed 5 x 1 hours with PBS at 4 ºC ...
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bioRxiv - Biochemistry 2024Quote: ... After blocking 25 µL was removed and 25 µL of primary antibody solution in 1% BSA and 0.3% Triton-X100 in PEM was added to stain the cell body and flagella overnight at 4°C (anti-tubulin, Abcam #ab6161, 1:1000 dilution). Due to the amount of time required for imaging ...
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bioRxiv - Physiology 2024Quote: ... Colonic sections were then incubated with primary antibodies overnight at 4°C followed by secondary antibodies (mouse IgG ab150113 Abcam, rabbit IgG ab96891 Abcam) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Fixed worms were washed three times in PBS for 5 mins and transferred to new 12-well plates with 1ml per well of 1x Phalloidin-iFluor 647 Reagent (Abcam cat# ab176759) in PBS pH=7.4 for 90 mins at room temperature with moderate shaking ...
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bioRxiv - Neuroscience 2021Quote: ... slices were then incubated at 4 °C for 5-7 days in the primary antibodies containing: chicken anti-glial fibrillary acidic protein (GFAP) (targeting astrocytes, 1:200, Abcam #ab4674, USA) and/or and NeuN (targeting nuclei of neurons ...
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bioRxiv - Pathology 2021Quote: ... The samples were then washed for 5 minutes three times with TBT and incubated with the secondary antibody (Thermofisher A-21121 and Abcam® ab97239) for an hour at a dilution of 1:200 ...
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Role of autophagy in sepsis-induced skeletal muscle dysfunction, whole-body metabolism, and survivalbioRxiv - Cell Biology 2021Quote: ... Membranes were blocked in PBS + 1% Tween® 20 + 5% BSA for 1 h at room temperature then incubated overnight with specific primary antibodies at 4°C.Membranes were washed in PBST (3×5 min) then incubated with HRP-conjugated goat anti-rabbit (ab6721) or rabbit anti-mouse (ab6728) secondary antibodies (Abcam, Cambridge, MA) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2022Quote: Sections were briefly dried then washed 3x in 0.1% Triton X-100 in phosphate buffered saline (PBST) before they were blocked with 5% neutral goat serum (Abcam or Vector Labs). Sections were then incubated for double-labeling experiments with primary antibody (AB ...
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bioRxiv - Systems Biology 2022Quote: ... Cells were tehen incubated with a primary antibody solution containing BrdU antibody diluted 1:400 or 1:1000 in PBS containing 5% goat serum (Abcam, catalog # ab7481) and 0.1% Triton-X for 1 hour at room temperature ...
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bioRxiv - Immunology 2021Quote: ... iPS cells were treated for 1 h with HDR enhancer (5 μM; IDT, Coralville, USA) and 10 μM Y-27632 (Abcam, Cambridge, UK). Electroporated cells were seeded on Laminin 521 (Biolamina ...
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bioRxiv - Immunology 2021Quote: ... Concentration of circulating LPS-binding protein (LBP) was determined in plasma samples (diluted 1:5) using Mouse LBP ELISA Kit (Abcam, Cambridge, MA) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... The membrane was then treated with a blocking solution for half an hour (5% skim milk in 1% TBST) and incubated with the primary antibody (ab138501, Abcam, Cambridge, UK) at a ratio of 1:5,000 overnight at 4°C under constant motion ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were washed three times with TBST for 5-min and then incubated for 1-hr with horseradish peroxidase-conjugated goat anti-rabbit IgG (ab6721, Abcam, Cambridge, MA). Membranes were washed three more times with TBST for 5-min.
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bioRxiv - Neuroscience 2024Quote: ... membranes were washed three times with TBST for 5-min and then incubated for 1-hr with horseradish peroxidase-conjugated goat anti-rabbit IgG (ab6721, Abcam, Cambridge, MA). Membranes were washed three more times with TBST for 5-min.
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bioRxiv - Neuroscience 2023Quote: ... Slices were washed in 2x SSC (3 x 5 min) and incubated in blocking solution containing secondary antibody (anti-chicken Alexa 488, Abcam, 1:1500) for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then washed three times for 5 minutes each with PBS and then stained with a 1:3,000 dilution of Phalloidin-555 Alexafluor (# ab176756, Abcam, Cambridge, United Kingdom) in 300 µl PBS for 30 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... which was prepared by 5% milk in TBS-T containing 1:10,000 horseradish peroxidase (HRP)-conjugated goat anti-mouse secondary antibodies (Abcam ab6789-1 MG). The secondary antibody incubation was applied for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... Stripped membranes were blocked in 5% NFDM in TBST (1 h, RT) before being probed with anti-Act1 (Abcam ab8224, 1:5000) loading control antibody (1 h ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... washed three times in 1XPBS for 5’ and incubated with anti-neural/glial antigen 2 (NG-2, 1:100, in 1XPBS + 1%NGS, Abcam, Cambidge, UK), overnight at 4°C.
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was blocked with 5% skim milk for 1 hour at RT before immunoblotting with antibodies against ACC1 (#ab72046, Abcam, 1:2000), ACC2 (#HPA006554 ...
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bioRxiv - Plant Biology 2023Quote: Immunoblotting was performed with antibodies diluted in the blocking solution (5% nonfat milk in TBS with 0.1% [v/v] Tween) at the following dilutions: α-GFP antibody (ab290, Abcam, Cambridge, UK), 1:8,000 ...
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bioRxiv - Genetics 2023Quote: ... was washed four times with 1X DPBS supplemented with 5 mg/mL BSA and subsequently crosslinked to 5 μg antibody (Anti-FOXA1 antibody: GeneTex, GTX100308; Anti-Fra2 antibody: Cell Signaling Technology, 19967S; Anti-H3K9me3 antibody: abcam, ab8898 ...
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bioRxiv - Molecular Biology 2023Quote: A pair of parotid salivary glands were harvested from each mouse (n=5/group) and directly placed in 500 uL of Lactate Assay Buffer from the L-Lactate Assay Kit (ab65331, Abcam, Cambridge, UK), 10 uL of protease inhibitor (PI ...
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bioRxiv - Pathology 2024Quote: ... was performed on 5 μm sections according to the previously reported protocol (Sellmer Ramos et al., 2023; ab108422; 1:500 dilution; Abcam; Waltham, MA) to identify adenomyotic foci in uterine cross-sections ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then washed with PBS 3x 5 minutes and incubated with secondary antibodies (goad anti-rabbit 488 or 555; Abcam, 1:1000) 2 hours at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... for 1h followed by antibody incubation in 5% Bovine Serum Albumin (BSA) in TBS-T overnight (TfR, 13-6800, ThermoFisher, 1:250; ApoE, Ab947, abcam, 1:500). After three times washing in TBS-T ...
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bioRxiv - Cancer Biology 2024Quote: ... anti-HA (16B12, Biolegends, 2.25 µg/60 µl beads/IP) or anti-Ago2 (2E12-1C9, Abcam, 5 µg/60 µl beads/IP). Lysates were added to the beads and incubated overnight at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... Membranes were probed with primary antibodies against PDK1 (1:2 500 in 5% skim milk/TBS-T, Cat. No. ab202468, Abcam, Cambridge, UK), β-tubulin (1:2 000 in 5% skim milk/TBS-T ...
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bioRxiv - Biophysics 2020Quote: ... The sample were incubated for 48 hrs at 4°C for secondary antibodies Alexa Fluor 647 (Donkey anti-Rabbit, Abcam ab 150075, 1:500 dilution) and Alexa Fluor 555 (Anti-mouse IgG (H+L) ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were then incubated in primary antibody for 48 hours at 4°C (ChAT (1:200): goat anti-ChAT (Abcam Cat. #ab144P, Millipore, Temecula, CA), PV (1:2000) ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were applied in blocking buffer at 4°C over-night (alpha-tubulin, Abcam ab233661, 1:1000 dilution and Pcdh19, Abcam ab191198, in 1:200 dilution). After 3 wash steps in PBS-T (0.1% triton) ...
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bioRxiv - Physiology 2020Quote: ... Blots were probed using an antibody against one subunit of each of the mitochondrial oxidative phosphorylation complexes (Abcam #ab110412; 1:1000; overnight at 4°C) and followed by fluorescent secondary (1:10,000 ...
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bioRxiv - Immunology 2021Quote: ChIP-seq was carried out using 30 μg of chromatin isolated from cryopreserved PBMC samples obtained from two CLL patients and 4 μl of anti-EGR2 antibody (Abcam, cat# ab43020, Lot# GR101477-1). The immunoprecipitated DNA was processed into a standard Illumina ChIP-seq library and sequenced on an Illumina HiSeq instrument (Illumina ...
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bioRxiv - Bioengineering 2022Quote: ... primary antibodies used were for MHC (Developmental studies hybridoma bank #MF-20, Iowa City, IA, USA; 4 μg/mL) and Phalloidin-488 (Abcam #ab176753, Cambridge, UK; 1:1,000). Following primary antibody incubation ...
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bioRxiv - Cell Biology 2024Quote: ... Immunoblotting was next performed by incubating the membranes overnight at 4°C with the following primary antibodies: mouse monoclonal anti-mitofusin 2 antibody [6A8] (abcam, ab56889, dilution of 1/1000), rabbit polyclonal anti-mitofusin 2 (abcam ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were blocked with 5% w/v skimmed milk in TBST for one hour and then incubated at 4□ with primary antibodies (p-PI3K, Abcam, Catalog#ab278545; p-AKT(S473), CST ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was blocked in 5% non-fat milk powder in PBS-T followed by overnight incubation at 4°C with primary antibodies (anti-GFP; Abcam ab290 1:1000, anti-GAPDH; Abcam ab9485 1:10000, Cambridge, UK) 3x 5 minute washes with PBS-T ...
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bioRxiv - Bioengineering 2024Quote: ... followed by an overnight incubation at 4°C with gentle shaking in primary antibodies against Gag at 1:2000 dilution (Abcam cat. no. ab100970, RRID: AB_10696216) and Actin at 1:5000 dilution (Abcam cat ...