Labshake search
Citations for abcam :
2051 - 2100 of 2500 citations for 6 Methyl 5 propyl 4 1H pyrimidinone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Eluted proteins were resolved on NuPAGE™ 4–12% Bis–Tris gel followed by Western blotting using anti-IFNAR1 antibody (ab124764, Abcam).
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bioRxiv - Bioengineering 2024Quote: ... Constructs were then incubated overnight at 4 °C with primary antibodies for collagen IV (1:200, rabbit IgG; Abcam, Waltham, MA) or laminin β-1 (1:200 ...
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bioRxiv - Cell Biology 2024Quote: ... followed by 72 hours incubation at 4°C with Rabbit recombinant monoclonal anti-mouse HIF-1 alpha antibody [EPR16897] (abcam, ab179483), dissolved in 0.5% BSA in PBS (1:500 dilution) ...
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bioRxiv - Cancer Biology 2024Quote: ... 4 µm-thick TMA slides were incubated with the primary CD14 monoclonal antibody (clone 1H5D8, Abcam, Cat# ab181470, Camdridge, MA, USA) at a dilution of 1:3,000 for 15 minutes at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... The 2 or 4 µm section was used for H&E staining and IHC of anti-human CD11b mAb (abcam #ab307523). The samples were observed by Nikon Eclipse Ti2 microscope carrying NIS-Elements imaging software.
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bioRxiv - Developmental Biology 2024Quote: ... in PBS for 1 h at RT and incubated with primary antibodies for at least 1 h at RT or overnight at 4°C (p300, Abcam, ab275388; CBP, Abcam, 253202; FLAG, Sigma, F1804; H3K27ac, Active Motif, 39133; H3K18ac, Abcam, ab40888 ...
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bioRxiv - Developmental Biology 2024Quote: ... in PBS for 1 h at RT and incubated with primary antibodies for at least 1 h at RT or overnight at 4°C (p300, Abcam, ab275388; CBP, Abcam, 253202; FLAG, Sigma, F1804; H3K27ac, Active Motif, 39133; H3K18ac, Abcam, ab40888; H2BK5ac, Abcam, ab40886 ...
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bioRxiv - Neuroscience 2020Quote: ... and a high concentration of detergent (Triton-X100, 5%) for at least two days before staining with 3,3’Diaminobenzidine (DAB, AbCam). Cells were then reconstructed and cortical layers delimited using Neurolucida 7 (MBF Bioscience ...
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bioRxiv - Biochemistry 2020Quote: ... The following primary antibodies were used by dilution in 5% nonfat milk PBST: anti-NRF2 (Abcam, ab62352, 1:4,000), anti-β-Actin-HRP (Huaxingbio ...
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bioRxiv - Neuroscience 2020Quote: ... The membranes were blocked using 5% skim milk for 1 h and then incubated with primary antibodies against CDKL5 (1:1,000, Abcam), K+/Cl- co-transporter 2 (KCC2 ...
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bioRxiv - Physiology 2020Quote: ... rinsed in PBS 2×10min and then incubated in blocking solution containing 5% normal goat serum (NGS, Ab7481, Abcam), 0.5% Triton (T8787 ...
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bioRxiv - Cell Biology 2021Quote: ... Pharmacological targeting of MLKL was achieved using in vitro treatment with the MLKL inhibitor necrosulfonamide NSA (Abcam, 5 µM).
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bioRxiv - Cell Biology 2021Quote: ... The sections were blocked in 5% skim-milk in PBS with 0.2% Triton-X prior to incubation with anti-von Willebrand factor (Abcam) and FITC-labeled anti-α-smooth muscle actin (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... The following antibodies were used for RNA-binding protein immunoprecipitation (RIP) assays: anti-H1.3 (ab24174, Abcam, 5 µg/reaction), anti-H1.5 (ab18208 ...
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bioRxiv - Neuroscience 2020Quote: Cell viability was measured after 3 days and 5 days of culture using an MTT [3-(4,5-dimethyldiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay (Abcam, ab211091) as per manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... 50 mL lysates was kept as input and the rest were incubated with 5 μg RAD21 antibody (Abcam, #ab992) or 10 μg CTCF antibody (Millipore ...
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bioRxiv - Neuroscience 2021Quote: ... and a high concentration of detergent (Triton-X100, 5%) for at least two days before staining with 3,3′Diaminobenzidine (DAB, AbCam).
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bioRxiv - Cancer Biology 2020Quote: ... Peroxide block was added for 5 min at room temperature followed by primary PD-L1 (0.154 μg/ml, clone SP142, Abcam) for 1 hr at RT ...
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bioRxiv - Neuroscience 2022Quote: ... previously washed with 5% BSA in TBS-Triton buffer and preincubated overnight with 2.5 μg anti-GFP antibody ab290 (Abcam). For the negative control ...
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bioRxiv - Immunology 2022Quote: Cell proliferation was measured using a 5-bromo-2-deoxyuridine (BrdU) labeling and a proliferation ELISA Kit (Abcam, ab126556) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... primary antibodies were incubated overnight in 5 % milk-TBST using the following concentrations: anti-HAS2 (1:500, Abcam, ab140671), anti-VCP (1:5000 ...
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bioRxiv - Molecular Biology 2021Quote: ... Coverslips were then stained with primary stress granule antibodies (5 μg/mL mouse α-G3BP primary antibody (ab56574; Abcam) or 1 μg/mL Rabbit α-PABP primary antibody (ab21060 ...
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bioRxiv - Neuroscience 2021Quote: ... and a high concentration of detergent (Triton-X100, 5%) for at least two days before staining with 3,3’-Diaminobenzidine (DAB, AbCam). Cells were then reconstructed ...
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bioRxiv - Molecular Biology 2021Quote: ... and those expressing GCGR were incubated with 1:50 anti-GCGR antibody (diluted with PBS containing 5% BSA, Abcam) at RT for 1 h ...
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bioRxiv - Biochemistry 2020Quote: ... Tissues were lysed by 5% Total glutathione content was measured using an Intracellular glutathione (GSH) Detection Assay Kit (Abcam) as described in the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... the membranes were blocked with 5% skim milk and incubated with anti-m6A antibody (ab195352, Abcam, 1:1000 dilution) overnight at 4 ℃ ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 1 hour in a blocking solution comprising PBS with 0.3% (weight/volume) Triton X-100 and 5% (weight/volume) donkey serum (Abcam) containing 1% (w/v ...
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bioRxiv - Biophysics 2021Quote: ... the samples were incubated in PBS with 5% BSA and Anti-alpha Adaptin primary antibody (Abcam, ab2730, 1:250) for 2 hours at RT ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were blocked at RT for 30 min in blocking solution (1x PBS3T 5% normal goat serum (Abcam ab138478)) and incubated in primary antibody dilution buffer (antibody diluted in 1x PBS3T and 1% BSA ...
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bioRxiv - Pathology 2023Quote: ... and then incubated for 1 hour in PB solution containing 5% normal goat serum (Abcam Cat# ab7481, RRID: AB_2716553). The sections were then incubated with the primary antibody ...
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bioRxiv - Molecular Biology 2023Quote: ... mRNAs were fragmented into about 100-nt fragments and immunoprecipitated (IP) with 5 μg m6A antibody (Abcam, Cambridge, UK). The antibody-RNA complex was isolated by incubation with protein A beads (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: Free-floating sections were washed in PBS and blocked with 5% normal donkey serum (ab7475, Abcam, Cambridge, United Kingdom) in PBS containing 0.1% Triton X-100 (PBS-T ...
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bioRxiv - Biophysics 2024Quote: ... The membrane was blocked in 5% milk in TBS-T buffer and probed with anti-CPEB4 antibody (Abcam ab224162).
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bioRxiv - Neuroscience 2024Quote: ... All chambers were again washed twice with PBS before a blocking solution consisting of 5 % goat serum (Abcam, ab7481) diluted in PBS was added to the cultures ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated with PBST (Triton 0.3 %) solution containing 5 % donkey normal serum and one or more antibodies (goat Anti GFP, ab6673, Abcam, diluted 1:2000 ...
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bioRxiv - Neuroscience 2024Quote: ... 0.1% Tween-20) and incubated overnight with primary antibodies diluted in 5% milk in TBST (anti-ELK1 (Abcam ab32106) 1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were blocked with 5% milk in 0.1M TBST and probed with primary antibody: GAPDH (Mouse, Abcam G8795,1:1000) was used as an internal control for the concentration of protein loaded ...
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bioRxiv - Pathology 2023Quote: ... Sections were washed 3 times for 5-10 min in 0.3% PBT and incubated with secondary antibodies and Hoechst (Abcam) in 3% NDS in 0.3% PBT for 1 h at RT ...
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bioRxiv - Microbiology 2022Quote: ... The membrane was blocked in 5% non-fat milk and probed with a 1:1,000 dilution of anti-Pyk2 (Abcam) or a 1:1000 dilution of anti-phosphorylated Pyk2 (ab4800 ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were washed twice with PBS containing 0.025% triton-x for 5 minutes and then blocked with protein block (ab64226, Abcam) for 20 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... Calcium transients were observed by incubating cerebral organoids for an hour with 8 µM Fluo-5 AM (Abcam; ab241083) in the ACSF ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibody incubation was performed over night at 4°C in a humidified chamber and the antibodies were diluted in antibody buffer (5% normal goat serum, PBS, 0.2% Triton X-100) (primary antibodies: Rabbit s100b, abcam, AB52642-1001 ...
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bioRxiv - Immunology 2024Quote: ... 5 µg/ml rat anti-mouse C3b/iC3b (clone 3/26, Hycult Biotec) and 5 µg/ml rabbit anti-mouse C1q (clone 4.8, Abcam). Secondary antibodies were added for 3h at RT ...
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bioRxiv - Neuroscience 2024Quote: ... then incubated with the primary antibody diluted in PBS containing 0.5% Tween-20 and 5% NGS (rabbit anti-mCherry antibody, Abcam, ab167453 ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated overnight at 4°C with the following primary antibodies diluted in 0.2% sodium azide+5% BSA in 1X PBS : Sortilin-related receptor 1 (SORLA) at 1:1000 (# ab190684; abcam); Mouse monoclonal anti-Actin (clone A4 ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated at 37°C for 30 min with complete DMEM containing 5 µM Zinpyr-1 (Abcam, ab145349), Hoechst 33342 (0.2 µg/mL ...
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bioRxiv - Neuroscience 2024Quote: ... The membrane was then washed (3 x 5 min) in TBST and incubated in secondary antibody (1:5,000, goat anti-rabbit HRP-conjugated secondary, Abcam) in 1% non-fat milk in TBST for 3 hrs at room temperature ...
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Spatial 3D genome organization controls the activity of bivalent chromatin during human neurogenesisbioRxiv - Neuroscience 2024Quote: ... Cells were incubated in antibody binding solution (5% NGS and 0.1% Triton X-100 in D-PBS) containing LaminB1 (Abcam) or SON (Atlas Antibodies ...
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bioRxiv - Biochemistry 2024Quote: ... before 5x 5 min washing (∼10 mL in TBS-T) and incubation with secondary Rabbit-HRP antibodies (ab79773, Abcam) for 60 min ...
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bioRxiv - Genetics 2024Quote: ... Standard 5 µm sections were prepared and stained for eosinophils (Anti-Ribonuclease 3/eosinophil cationic protein antibody, Abcam, Ab207429). The presence of ECRSwNP was determined by counting the number of eosinophils per high-power field (HPF) ...