Labshake search
Citations for abcam :
1951 - 2000 of 2500 citations for 6 Methyl 5 propyl 4 1H pyrimidinone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... the membranes were then probed at 4°C overnight with various primary antibodies: anti-foxa2 (Seven Hills) or LXR-α (Abcam), washed with TBST (20 mM Tris ...
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bioRxiv - Physiology 2021Quote: ... Membranes were blocked in 3% skim milk for 2 hours and then incubated with primary antibody overnight at 4°C for the following proteins: 4HNE (Abcam, ab46545), β-actin (Santa Cruz Biotech) ...
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bioRxiv - Physiology 2020Quote: ... Membranes were incubated overnight at 4 °C with the indicated primary antibodies diluted in blocking solution: NT3 (1:1000, Abcam ab65804), TrkC (1:750 ...
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bioRxiv - Physiology 2020Quote: ... Sections were incubated overnight in a humidity chamber at 4°C in a mixture of rabbit anti-androgen receptor (Spring Bioscience m4070) and chicken anti-tyrosine hydroxylase (Abcam ab76442) in NGS/TBS/BSA ...
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bioRxiv - Physiology 2020Quote: ... or guinea pig anti-glucagon (PCA2/4, 1:200; raised in-house) with rabbit anti-Ki-67 (1:200; Abcam ab15580) or TUNEL reaction mixture (Roche Diagnostics Ltd ...
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bioRxiv - Developmental Biology 2021Quote: Cardiomyocytes were fixed with 4% formaldehyde solution in PBS for 10 min and stained overnight at 4 °C with primary antibodies against TNNI3 (1:500, Abcam, ab56357) and Ki67 (CellSignaling ...
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bioRxiv - Cell Biology 2020Quote: ... the ovarian samples were incubated overnight at 4°C with primary antibodies against caspase-3 (1:1000 dilution, Abcam, Cambridge, MA) and NLRP3 (1:1000 dilution ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then blocked with 5% Bovine Serum Albumin for 30 min and incubated overnight at 4°C with anti-RNAPII phospho S5 (1:1500, Abcam, ab5408), anti-RNAPII phospho S2 (1:3000 ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by overnight incubation at 4 °C in 1% BSA with primary antibodies: mouse monoclonal [EA-53] to sarcomeric alpha-actinin (Abcam ab9465), rabbit polyclonal anti-phospho-Histone H3 (Ser10 ...
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bioRxiv - Immunology 2021Quote: ... Coverslips were incubated overnight at 4 °C in blocking buffer and probed the following morning with 1 :500 rabbit anti-ACE2 (Abcam ab15348) for 1h at room temperature on an orbital rocker ...
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bioRxiv - Biophysics 2020Quote: ... A negative control was performed on a subset of the wells by treating cells with a spike of 10 µM Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP, Abcam ab120081) 10 minutes prior to the application of TMRE ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were incubated with antibodies for one hour at 4°C in an overhead tumbler before immune complexes were retrieved by protein G (GE Healthcare or abcam) or A Sepharose (GE Healthcare) ...
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bioRxiv - Neuroscience 2023Quote: ... After SDS-PAGE and proteins transferred on PVDF membranes were incubated overnight at 4°C with Total OXPHOS Human WB Antibody Cocktail (Abcam, UK) and subsequently detected with HRP-conjugated goat anti-rabbit IgG secondary antibody (1:4000 ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were blotted overnight at 4°C with the following primary antibodies: total OXPHOS human antibody cocktail (Abcam, 1:5000 dilution), TOM20 (Santa Cruz Biotechnology 1:5000) ...
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bioRxiv - Biochemistry 2023Quote: Rat sera were incubated at 4°C for 16 h in the presence of anti-adiponectin antibodies from Abcam (ab 56416) and Cell Signaling (2789s) ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated in PBS with 3% donkey serum and the following primary antibodies for 72 h at 4°C: goat anti-Iba1 (1:500; Abcam, ab5076), rabbit anti-ACSL1 (1:100 ...
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bioRxiv - Developmental Biology 2023Quote: ... the sections were incubated overnight at 4°C in a blocking-permeabilizing solution containing rabbit anti-SOX10 (1:100, Abcam, ab227680). The next day ...
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bioRxiv - Developmental Biology 2023Quote: ... and then incubated overnight at 4 °C with rabbit anti-Bru1 (1:500) (38) or rabbit anti-GFP (1:1000, Abcam ab290). All antibodies are listed in the Resources Table S5 ...
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bioRxiv - Cancer Biology 2023Quote: ... Beads were removed and nuclear extracts were incubated overnight at 4°C with either anti-lamin A/C antibody (Abcam, #ab108595) or non-immune IgG ...
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bioRxiv - Cancer Biology 2023Quote: ... and sheared chromatin was incubated at 4°C overnight with 4µg of primary β-catenin (CST, D10A8) or SOX2 (Abcam, ab97959), or isotype control antibodies (rabbit IgG ...
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bioRxiv - Neuroscience 2023Quote: ... Cryosections then were incubated at 4°C overnight with the following primary antibodies at these concentrations: aSyn (Abcam ab209420, 1:1000), phosphorylated aSyn (Wako 015-25191 ...
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bioRxiv - Physiology 2023Quote: ... Frozen sections were fixed with fresh 4% paraformaldehyde and neutrophil elastase protein was detected using anti-neutrophil elastase antibody (Abcam #68672).
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bioRxiv - Molecular Biology 2023Quote: ... which was performed overnight at 4°C by incubating the shared DNA-protein complex with 10 µg of anti-ADNP (Abcam Ab231950) antibody ...
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bioRxiv - Molecular Biology 2023Quote: ... in methanol and non-specific binding blocked with 10% serum from the source species for the secondary antibody followed by a primary antibody incubation overnight at 4 °C (Sox9, abcam, ab185966,; p16, (abcam, ab241543), α-SMA (Sigma-Aldrich’ A5691) ...
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bioRxiv - Physiology 2022Quote: ... 0.5% mouse serum in PBS) for 1 h and stained overnight (4°C) in a humid chamber for α1B-adrenergic receptor (Abcam, ab169523). The following day sections were washed in wash buffer 3 times for 5 min and co-stained for anti-Rabbit IgG Fab2 Alexa Fluor 488 (Cell Signaling ...
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bioRxiv - Neuroscience 2022Quote: ... Incubation with primary antibodies diluted in PBS with 5 % NGS and 0.3 % Triton X-100 was carried out at 4°C overnight using rabbit anti-DCX (ab18723, Abcam, 1:1000), mouse anti-parvalbumin (p3088 ...
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bioRxiv - Molecular Biology 2022Quote: ... The membranes were incubated overnight with primary antibodies diluted in 1% BSA-TBST at 4°C using the following concentrations: anti-PAD4 (1:1000; Abcam; ab214810), anti-PAD2 (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then processed for immunostaining by overnight incubation at 4°C in the primary antibodies of anti-Human phospho alpha-synuclein (Ser129) (1:2000, Cat# ab51253, Abcam, RRID:AB_869973), anti-Iba-1 (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... Immunoblotting was done overnight at 4°C with the following antibodies at the appropriate dilutions: TRPC6 antibody (Abcam ab228771, 1:1000). The blots were washed the next day and incubated with Goat anti-Rabbit-HRP secondary antibody (Cat # 31460 ...
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bioRxiv - Cell Biology 2022Quote: ... the membrane was blocked using the Odyssey blocking buffer (LiCor) and incubated overnight at 4 °C with the primary antibody (SHIP2 antibody (Abcam #ab70267) 1/2000 ...
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bioRxiv - Bioengineering 2024Quote: ... cells were immuno-stained overnight at 4-8°C with rabbit anti-human IRF1 antibody (1:1000, ab243895, Abcam, Cambridge, UK) or rabbit anti-human pSTAT1 (1:400 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed twice with PBS before incubation overnight at 4°C with rabbit anti-aromatase (1:2000, Abcam cat # ab18995), chicken anti-TH (1:2000 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed twice with PBS before incubation overnight at 4°C with chicken anti-TH (1:2000, Abcam cat# ab76442) in 1% NGS + 0.05% Triton X-100 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The membrane was blocked for nonspecific binding with 5% milk in 1x TBST (Tris-buffered saline, 0.1% Tween 20) and incubated with rabbit polyclonal anti-4-HNE (ab46545, Abcam, 1:1500) and HIF-1α (141795 ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were then incubated at 4°C overnight in blocking buffer with the following antibodies and concentrations: chicken anti-GFP (1:1000; Abcam ab13970), rabbit anti-per (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... the slides were incubated overnight at 4°C with primary antibodies (anti-GFAP 1:1000, Invitrogen #PA1-10004; anti-ALDH1L1 1:1000, Abcam ab87117) diluted in PBS with 1 % bovine serum albumin (Winset Inc ...
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bioRxiv - Cell Biology 2024Quote: ... some of the following primary antibodies were incubated 24 hours at 4 °C: rabbit anti-SOX2 (1:100 dilution, Abcam, ab137285), rabbit anti-doublecortin (DCX ...
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bioRxiv - Cell Biology 2024Quote: ... incubated for 24 hours at 4 ° C with the following primary antibodies: rabbit anti-syntenin 1 (1:2000 dilution, Abcam, ab133267) and mouse anti-tubulin α (1:2000 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... The membranes were blocked in 5% lowfat milk-PBS-tween solution and then incubated overnight at 4 °C with primary antibodies: anti-PURA 1:1500 (rabbit—ab125200 abcam®), anti-GLUT1 1:2000 (mouse— [GLUT1/2476] (A249979 ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions were analyzed by separation using sodium dodecyl sulfate polyacrylamide gel electrophoresis (4–12% polyacrylamide) and staining with InstantBlue Coomassie Protein Stain (Abcam, UK). Fractions containing chalcone synthase were combined and loaded onto a HiLoad 16/600 Superdex 200 pg column ...
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bioRxiv - Microbiology 2024Quote: ... which was then washed in PBS with 0.1% Tween-20 (PBS-T) and incubated overnight at 4°C shaking in PBS-T with rabbit anti-GAC antibodies (Abcam ab21034) at a 1:5,000 dilution and mouse anti-His monoclonal antibody (Thermo Fisher Scientific or Abcam ab216773 ...
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bioRxiv - Neuroscience 2023Quote: ... and membranes were blocked with 5% skimmed milk in TBST buffer for 1 h and incubated overnight at 4 °C in TBST with the following primary antibodies: Anti-rabbit TOMM20 (Abcam #ab186735) 1:1000 ...
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bioRxiv - Cancer Biology 2024Quote: ... 20 μL of GBO medium containing 1 mM ACh with or without a combination of the M3 muscarinic receptor antagonist 4-DAMP (100 μM) and pan-nicotinic receptor antagonist mecamylamine (100 μM, Abcam, ab120459) was added to each GBO at the ALI surface 30 minutes prior to imaging with the same conditions ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated overnight at 4°C in PBS with primary antibodies including: chicken-anti-GFP (1:10000, Abcam, ab 13970) or rabbit-anti-dsRed (1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Then cells were washed three times with IF buffer and incubated overnight at 4°C with the following antibodies : 53BP1 (abcam, ab36823) or Rabbit IgG Isotype Control (CellSignaling ...
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bioRxiv - Cancer Biology 2023Quote: ... were subjected to overnight incubation at 4°C with a rabbit monoclonal anti-LMO3 antibody (Abcam, Cambridge, UK, ab230490, 1:500). Signal amplification was achieved using the Dako envision+ system-HRP labeled polymer anti-rabbit antibody (Agilent Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue was then incubated overnight at 4 °C on a shaker in primary antibodies against PGP9.5 (from rabbit; Abcam, catalog # EPR4118) and tdTomato (from goat ...
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bioRxiv - Genetics 2023Quote: ... Coverslips were incubated overnight at 4°C in PBS+ with primary antibodies: mouse monoclonal anti-smooth muscle actin (SMA) (1:750, Abcam, ab7817) and rabbit polyclonal anti-SM22 (1:400 ...
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bioRxiv - Microbiology 2023Quote: ... staining was performed overnight at +4⁰C with antibodies to the following targets: influenza A virus (IAV) polyclonal antibody (1:400, goat, Abcam, ab20841), NP (IAV ...
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bioRxiv - Neuroscience 2023Quote: ... 0.1% BSA and 1% normal goat serum overnight at 4°C with primary antibodies: rat anti-Ctip2 (1/500; Abcam, #ab18465), rabbit anti-Cux1 (1/500 ...