Labshake search
Citations for abcam :
2001 - 2050 of 2500 citations for 6 Methyl 5 propyl 4 1H pyrimidinone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Nuclei were washed twice with Wash buffer (w/o TritonX-100) and incubated overnight at +4°C with the following antibodies: Rb x NeuN-Alexa647 (1:400, Abcam #ab190565), Rb x NeuN-Alexa594 (1:400 ...
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bioRxiv - Cell Biology 2023Quote: ... This viral supernatant was then concentrated by centrifugation at 3200 x g for 30 minutes at 4°C before being resuspended in 100 µl virus suspension solution (Abcam, ab102538), aliquoted and stored at -70°C ...
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bioRxiv - Bioengineering 2023Quote: ... Hydrogels were blocked with 2% BSA-PBS solution for 1 hr at room temperature and incubated with one or more of the following primary antibodies diluted in 2% BSA-PBS at 4 °C for 48∼72 hr: rabbit anti-Collagen VI (1:200; Abcam, ab182744), rabbit anti-fibronectin (1:200 ...
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bioRxiv - Genetics 2022Quote: ... 500 ug lysate was incubated at 4□ overnight with 2 ug of appropriate antibodies (anti-H3K4me3 (Abcam, Cat# ab8580, RRID: AB_306649), anti-H3K36me3 (Abcam ...
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bioRxiv - Cancer Biology 2022Quote: ... for 1 h and then incubated overnight at 4 °C with primary antibodies: rabbit anti-human IRS2 (ab134101; Abcam, Cambridge, UK) and mouse anti-human active β-catenin (05-665 ...
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bioRxiv - Cell Biology 2022Quote: ... overnight at 4°C, and with fluorescence (Alexa Fluor 488, 568, 647)-conjugated secondaries (Jackson ImmunoResearch Laboratories or Abcam; 1:1000) overnight at 4°C at indicated dilutions ...
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bioRxiv - Cell Biology 2022Quote: Grown BEC-organoids were treated with the FA-mix for 4 days and triglyceride levels were measured with a Triglyceride kit (Abcam, ab65336) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... The slides were incubated with primary rabbit anti-SARS nucleocapsid protein antibody(NB100-56576, Novus, USA) overnight at 4°C and then with goat anti-rabbit IgG HRP (Abcam, UK) for 1 hr ...
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bioRxiv - Physiology 2022Quote: ... slides were incubated overnight at 4°C in primary antibodies (1:500 dilution in blocking solution) for glucagon (Abcam, cat. # ab10988) and insulin (Cat ...
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bioRxiv - Molecular Biology 2022Quote: ... milk at room temperature and incubated overnight at 4°C with the following primary antibodies: rabbit anti-Notch1 (1:1000, Abcam, ab8925), rabbit anti-Hes1 (1:1000 ...
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bioRxiv - Plant Biology 2022Quote: ... After three washes with 1 x PBS the samples were incubated for 4 hours with anti-mouse secondary antibodies conjugated with Alexa Fluor 488 (ab150077, Abcam, UK) diluted 1:400 in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... Anti-phospho Syn S129 (1:500, ab-59264), and anti-4-HNE (1:2000, ab-46545) were from Abcam (Cambridge, UK). Anti-α-Syn (1:1000 ...
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bioRxiv - Pathology 2023Quote: ... Sections were incubated with primary antibodies O/N at 4°C (Rabbit anti-human Lyve-1, Fitzgerald; Goat anti-murine Lyve1, R&D AF2125; rabbit anti-CD31, abcam Ab28364) and washed three times in PBS ...
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bioRxiv - Genetics 2022Quote: ... Around 20 μg or 10 μg of chromatin were incubated overnight at 4°C with 10 μg H3.X/Y or 2.5 μg H3K9me3 antibody respectively (Active Motif-61161, Abcam-ab8898). Chromatinantibody extracted with incubated with protein G Dynal beads (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... Pellets were washed and resuspended in PBS and subsequently incubated with 4 μg/ml of PE-conjugated GABARAP/GABARAPL1/GABARAPL2 antibody (Abcam, ab223948) for 1 h ...
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bioRxiv - Cell Biology 2022Quote: ... 0.1% Triton X-100) and incubated overnight at 4°C with primary antibody: anti-αSMA (1:100, Abcam cat. no. ab7817), anti-Periostin (1:50 ...
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bioRxiv - Cancer Biology 2023Quote: ... The resulting chromatin was incubated overnight at 4°C with histone modification antibodies (H3K27ac, Abcam ab4729; H3K27me3, Diagenode C15410195 and H3, Abcam ab1791) pre-conjugated with Dynabeads Protein G ...
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bioRxiv - Cancer Biology 2022Quote: ... The following antibodies were added at 1:200 dilution in PFS and incubated overnight at 4°C with gentle shaking: Collagen IV (Abcam ab19808), pAKT pS473 (CST ...
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bioRxiv - Biochemistry 2023Quote: ... Denatured samples were resolved in 4-12% Bis-Tris SDS-PAGE gradient gels and stained with InstantBlue® Coomassie protein stain (Cat#ab119211, abcam). Protein bands were excised from gel ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies were diluted in blocking reagent and incubated overnight at 4 °C (Collagen I (# ab34710, Abcam, Collagen IV (#ab6586, Abcam), MUC-1 (MA1-06503 ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were incubated overnight at 4°C with primary antibody (VRK3 #HPA056489 Sigma, B-actine #51255 Cell signaling, Lamin # ab16048 Abcam, H3S10P #05-806 Millipore ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections were blocked in 10% FCS and then incubated overnight at 4°C with the following primary antibodies diluted in 2% FCS: LGALS1 (Abcam ab138513), RBP1 (Invitrogen PA528713) ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were incubated overnight at 4 °C with the following primary antibodies (1:100, in blocking buffer): anti-CD146 (Abcam #ab75769); anti-#-SMA (Abcam #ab5694 ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was incubated overnight at 4°C with primary antibodies phospho-STAT3-Tyr705 (Cell Signalling, 9145S), STAT3 (Cell Signalling, 4904S) and Twist1 (Abcam, ab50887) raised in rabbit ...
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bioRxiv - Developmental Biology 2024Quote: ... in PBS for 2 hours at room temperature and incubated overnight at 4°C using the following concentrations: anti-H3K9me3 (1:300, Abcam ab8898), anti-H3K27me3 (1:300 ...
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bioRxiv - Neuroscience 2024Quote: ... Equal aliquots of sonicated chromatin were used per immunoprecipitation reaction with 4 ul H3K27ac antibody (Abcam, cat #4729; lot # GR323132-1) preconjugated to Protein G Dynabeads (Life Technologies) ...
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bioRxiv - Microbiology 2024Quote: ... membranes were stained at 4 °C with the following primary antibodies at 1:1000 dilutions: tumor necrosis factor alpha (TNF-α, ab66579, Abcam), interleukin-1 beta (IL-1β ...
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bioRxiv - Genomics 2024Quote: ... blocked with blocking solution (PBS + BSA 4% + 0.025% Tween 20) and incubated with an anti-p65 antibody (1:200 Abcam Cat #ab16502) overnight at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... Sections were then incubated in primary antibody overnight at 4°C and the following primary antibodies were used: Sox10 (1:500, Abcam #ab155279), phospho-histone H3 (1:500 ...
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bioRxiv - Cancer Biology 2024Quote: ... and samples were incubated overnight at 4°C with one of the following: A 1:200 dilution of PAK4 (Abcam, ab62509), E-cadherin (Cell signaling ...
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bioRxiv - Biophysics 2024Quote: ... proteins were transferred to a nitrocellulose membrane (Li-Cor, 926-31092) and incubated overnight at 4°C with primary antibodies: Mic60/mitofilin (Abcam, ab110329). The membrane was incubated for 1 hour at room temperature with secondary antibodies ...
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bioRxiv - Bioengineering 2024Quote: ... to deionized water and fixed in 4% PFA for 15 min before staining with picrosirius red according to the manufacturer’s instructions (Abcam, cat#: ab150681). Immunostaining ...
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bioRxiv - Physiology 2024Quote: ... Protein was then transferred to a nitrocellulose membrane (Li-Cor 926-31092) that was incubated with primary antibodies overnight at 4°C: Mic60/mitofilin (Abcam ab110329), SAM50 (Proteintech 20824-1-AP) ...
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bioRxiv - Neuroscience 2024Quote: ... for 1hr at room temperature and incubated overnight at 4°C with primary antibodies directed against pS129 α-synuclein (rabbit, 1:10,000, EP1536Y, Abcam; #ab51253), tyrosine hydroxylase (chicken ...
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bioRxiv - Biochemistry 2024Quote: ... Membranes were incubated for 2h (at RT) or 24h (at 4°C) with anti-6X His-tag antibody (Abcam, Cat#ab18184) or anti-CLN5 antibody (Abcam ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were transduced with the Sh2 or Scr control constructs on 4 DIV and cellular ATP measurements were performed on 18 DIV using a Luminescent ATP Detection Assay Kit (Abcam, ab113849). To block luminescence signal contamination from adjacent wells ...
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bioRxiv - Physiology 2024Quote: ... Microglia were labelled for Iba1 (Ionized Calcium-binding Adapter Molecule 1) by incubating the slices for 48 hours at 4°C with a rabbit monoclonal anti-Iba1 antibody (Abcam, France), rinsed 3 times ...
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bioRxiv - Neuroscience 2024Quote: ... mouse monoclonal antibody in the dilution of 1:4000 was used with incubation at 4⁰C for 24hrs and secondary Goat anti mouse HRP (abcam 205719) was used in a dilution of 1:5000 with 2hrs incubation at RT ...
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bioRxiv - Neuroscience 2024Quote: ... The primary antibody solution was applied to tissue sections overnight at 4°C and consisted of chicken anti-GFP (1:1000; Abcam, ab13970) diluted in TBS buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... (pH 7.5)] for 1 hour and then incubated with the appropriate diluted primary antibody at 4°C overnight: TSPO (1:5000; Abcam, ab108489), actin (1:2000 ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were incubated with primary antibodies overnight at 4°C in blocking buffer at the following dilutions: anti-ALDH1L1 (1:70; Abcam ab87117), anti-tubulin β-III (1:5000 ...
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bioRxiv - Neuroscience 2024Quote: ... for 1 hour and then incubated overnight at 4°C with primary antibodies TSG101 (1:1000, ab30871, Abcam, Cambridge, United Kingdom), ALIX (1:1000 ...
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bioRxiv - Genomics 2024Quote: ... 16.7 mM Tris pH 8 and 167 mM NaCl) and incubated overnight with an H3K27ac antibody with rotation at 4 °C (Abcam, ab4729). Protein G Dynabeads (Thermo Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... Sections were then incubated overnight at 4°C in a primary antibody solution consisting of chicken anti-GFAP (1:1000, Abcam, ab4674), rat anti-CD86 (1:500 ...
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bioRxiv - Biophysics 2024Quote: ... Nuclear pore complex protein Nup133 or β-tubulin were labeled overnight at 4°C with rabbit anti-Nup133 antibody (Abcam, #ab155990) and mouse anti-human β-tubulin mAb (Sigma-Aldrich ...
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bioRxiv - Synthetic Biology 2024Quote: ... at room temperature for 20 minutes followed by incubation at 4°C overnight with mouse anti-ATF3 (1:100, Abcam ab191513) in a humidified chamber ...
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bioRxiv - Cancer Biology 2024Quote: ... the membranes were probed with the following primary antibodies overnight at 4°C: anti-CD9 clone EPR2949 (1:100, Abcam, 92726), anti-CD81 clone B11 (1:100 ...
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bioRxiv - Biochemistry 2024Quote: ... The membranes were re-hydrated in PBS for 1h at RT before being incubated overnight at 4°C with polyclonal human LAMP2A antibody hosted in Rabbit (1:1000; ab18528; Abcam, USA). After washing ...
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bioRxiv - Cell Biology 2024Quote: ... followed by an overnight incubation at 4°C with Rabbit recombinant monoclonal anti-mouse Collagen type I antibody [EPR24331-53] (Abcam, ab270993), dissolved in the blocking solution (1:1000 dilution) ...
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bioRxiv - Biochemistry 2024Quote: ... and then incubated overnight at 4°C with primary antibodies: Anti-beta Amyloid 1-40 [EPR23712-2] (AbCam, Cat. No. ab289991) for Aβ40 and Anti-Amyloid Beta [21F12] (Absolute Antibodies ...