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Citations for abcam :
2251 - 2300 of 2500 citations for 6 Methyl 5 propyl 4 1H pyrimidinone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated overnight at 4⁰C on a plate rocker in a 5% NGS/0.1% PBS-Triton solution containing rabbit anti-cFos antibody (Abcam, ab190289) at 1:500 dilution ...
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bioRxiv - Neuroscience 2024Quote: ... the membrane was washed in TBS-T three times for 5 minutes each and incubated with goat anti-mouse IgG HRP (1:5000, cat# AB205719, Abcam) in TBS-T for 1 h at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then permeabilized in 1mL of 0.1% Triton X-100 in 1X PBS for 5 min at room temperature and incubated with anti-DDX5 primary antibody (rabbit, ab21696; Abcam) diluted to 1:400 in PBS 1X for 1 hour ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The slices were blocked with 5% goat serum (Beyotime, China) for 30 min and then incubated with anti-Aβ1-42 (1:300, Abcam, UK), anti-Tau(pSer396 ...
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bioRxiv - Biochemistry 2023Quote: ... samples were washed with 5 mL of 0.1% Triton X-100/PBS and incubated with 1 μL of IRF5 antibody (Abcam, 181553) in 100 μL of 0.1% Triton X-100/PBS for 2 h at RT ...
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bioRxiv - Cell Biology 2023Quote: ... Paraffin-embedded tissue was sectioned (5 µm) and stained with control IgG or ANO1 antibodies (Abcam # ab53212, 3 µg/ml) by the University of Chicago Immunohistochemistry Core Facility.
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bioRxiv - Microbiology 2022Quote: ... blocked for 1 h using 5% donkey serum and then incubated with a rabbit monoclonal H3Cit antibody (citrulline R2 + R8 + R17, clone EPR20358-120; Abcam) at 1:500 dilution overnight in a humidified chamber at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Subsequently the membrane was incubated with the rabbit anti-IMPA1 antibody (1:10000 diluted in 5% BSA in 0.1% TBST) (Abcam ab184165) or goat anti-CHRM1 antibody (1 µg/ ml ...
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bioRxiv - Genomics 2023Quote: ... The remaining supernatant was transferred to a 2 mL round-bottom tube and incubated with 5 μg/μL of anti-GFP antibody (ab290; Abcam) at 4°C with end-over-end rotation for one hour ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue sections were rehydrated with PBS for 5 minutes before blocking and permeabilization for 1 hour with 10% normal goat serum (Abcam) containing 0.3% PBS-TritonX-100 ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was blocked using TBS-T with 5% BSA for 1 hour at room temperature and incubated using p-SMAD3 (ab52903, Abcam), type I collagen (1310-30 ...
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bioRxiv - Neuroscience 2023Quote: ... washed three times with PBS for 5 min each and incubated with secondary antibody Alexa Fluor® 647 (Abcam, ab150067) for an hour at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... and 12.5 uL (12.5 ug) of MHC class II antibodies in a 3:1:1 mixture of TAL-1B5 (Abcam, ab20181), EPR11226 (Abcam ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 5 μL of Annexin V-FITC and 5 μL of propidium iodide were added into each tube (Annexin V-FITC Apoptosis Staining/ Detection Kit, Abcam). The tubes were incubated at RT for 5 min in the dark ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated at RT for 1hr in secondary antibody diluted in TBST-5% milk (1:4000 ab6721 Goat Anti-Rabbit IgG H&L HRP (Abcam), 1:4000 ab205719 Goat Anti-Mouse IgG H&L HRP (Abcam)) ...
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bioRxiv - Cancer Biology 2023Quote: The 5-ethynyl-2’-deoxyuridine (EdU) incorporation assay was performed using the EdU Proliferation Assay Kit (iFluor 488) (ab219801, Abcam). Cells were seeded onto coverslips and incubated with 10 μM EdU for 4 h at 37°C in a humidified atmosphere with 5% CO2 ...
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bioRxiv - Immunology 2023Quote: Paraffin sections (5 μm) from lung tissues were processed using standard protocols for immunohistochemistry (IHC) staining according to manufacturer’s instructions (Abcam: ab236468). Tissues were permeabilized by incubation in 0.4% Triton-X in TBS for 20 min ...
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bioRxiv - Bioengineering 2023Quote: ... was used to separately conjugate an anti-tau antibody (Tau-5, AB_2721194) and anti-α-synuclein antibody (MJFR1, Abcam, ab138501) to dibenzocyclooctyne (DIBO ...
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bioRxiv - Molecular Biology 2023Quote: ... for 1 h prior to incubation in primary antibody in TBST with 5% skim-milk (rabbit anti-GFP 1:10,000; Abcam - ab290) overnight ...
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bioRxiv - Pathology 2023Quote: ... Sections were washed 3 times for 5-10 min in PBT and incubated for 2 h at RT in secondary antibody and Hoechst (Abcam) in 3% NDS in PBT ...
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bioRxiv - Cancer Biology 2023Quote: ... Antigen retrieval was accomplished by a 5-minute pressure cook of the slide submerged in 1X HIER buffer (abcam ab208572). Deionized water was slowly added to the pressure-cooked slide in HIER buffer ...
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bioRxiv - Cell Biology 2023Quote: ... sections were blocked with 10% goat serum and then stained with Keratin 5 or Keratin 8 together with GFP antibody (Abcam) in 10% goat serum for overnight at 4□ ...
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bioRxiv - Biochemistry 2023Quote: ... Slides were washed in TBS (3 x 5 min) prior to incubation with biotinylated goat anti-rabbit antibodies (Abcam, ab6720) in NGS (1:200 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were blocked with 5% donkey serum in 1x PBS for 1 hour and incubated with UFM1 (Abcam 1:100) and MC1 (1:1000 ...
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bioRxiv - Molecular Biology 2024Quote: ... Wells were again washed three times for 5 minutes with dPBS before incubating with secondary antibodies (goat anti-rabbit IgG Alexa Fluor 488 [Abcam] ...
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bioRxiv - Cell Biology 2024Quote: ... The membranes were blocked with 5% milk followed by incubation with primary antibodies overnight using the following antibodies: anti-VEGF-A (Abcam), anti-BCL2 (Cell Signaling Technology) ...
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bioRxiv - Cancer Biology 2024Quote: ... or with recombinant TGF-β at 10 ng/mL (stock concentration 5 ng/µL) or Batimastat at 30 µM (stock concentration 1 mM, BB-94/Abcam). After 48 hours (or 1 hour for SMAD activity tests) ...
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bioRxiv - Cell Biology 2024Quote: ... was detected in A549 cells fixed with 1% paraformaldehyde for 5 min using an anti-MT1-MMP rabbit monoclonal antibody (1:100 dilution, EP1264Y, Abcam). F-actin was stained with Alexa Fluor 350- or 568-conjugated phalloidin (Molecular Probes ...
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bioRxiv - Microbiology 2024Quote: ... Serine racemase treatment of human serum was performed with the addition of 5 µl of proprietary serine racemase solution from a DL-serine assay kit (Abcam) to 1 ml of serum for 3 h ...
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bioRxiv - Physiology 2024Quote: ... Sections were placed in 100% propylene glycol for 5 minutes then incubated in pre-heated Oil Red O (Abcam #150678) for 6 minutes at 60°C ...
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bioRxiv - Microbiology 2024Quote: ... The membrane was incubated with the primary antibody (RPS3, abcam, Cat#ab128995; eIF4E, abcam, Cat#ab33768; ORF3a, abcam, Cat#280953; 5-mC, Cat#ab214727; IFN-beta, abcam, Cat#ab275580 ...
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bioRxiv - Microbiology 2024Quote: ... unskimmed milk powder, and incubated with mouse monoclonal anti-HA (clone 6E2, Cell Signalling Technology) and anti-p24 (clone 5, Abcam) overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... To test for O-x-AlkTDM presence we used in-gel fluorescence (to detect TAMRA) and/or immunoblotted with anti-biotin antibodies (1:1000; 5% nonfat Milk in TBST; Abcam).
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bioRxiv - Molecular Biology 2024Quote: ... 3 µl of 1 mM DCFDA dye in 1X buffer and 5 µl of 10 µM DiOC2 was added for ROS evaluation (ab113851, Abcam) and membrane depolarization (B34950 ...
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bioRxiv - Microbiology 2024Quote: Anti-HIV-1 Pr55Gag + p24 + p17 antibody (ab63917) and anti-tubulin antibodies (B-5-1-2) were purchased from Abcam and Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... cells were incubated with 20 μM NE for 5 min and an aliquot of saline was used to measure adenosine using an adenosine assay kit (Abcam) and a microplate reader (Perkin Elmer Enspire ...
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bioRxiv - Molecular Biology 2024Quote: ... Blots were subsequently washed 3 times for 5 minutes each in PBST at room temperature before incubation in 5µg/mL of HNRNPA2B1 polyclonal primary antibody (abcam 31645) in PBST overnight at 4°C with agitation ...
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Decreased Astrocytic CCL5 by MiR-324-5p Ameliorates Ischemic Stroke Injury via CCR5/ERK/CREB PathwaybioRxiv - Neuroscience 2024Quote: ... The PVDF membranes were blocked with 5% BSA and probed with primary antibodies (phospho-ERK1/2, Abcam, ab76299; ERK1/2, Abcam, ab184699 ...
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Decreased Astrocytic CCL5 by MiR-324-5p Ameliorates Ischemic Stroke Injury via CCR5/ERK/CREB PathwaybioRxiv - Neuroscience 2024Quote: ... The PVDF membranes were blocked with 5% BSA and probed with primary antibodies (phospho-ERK1/2, Abcam, ab76299; ERK1/2, Abcam, ab184699; phospho-CREB, Abcam, ab32096 ...
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bioRxiv - Immunology 2024Quote: ... Cells were permeabilized with 0.1% Triton X-100 / 2% BSA in 1x PBS for 5 min and F-actin was stained by Phalloidin-iFluor488 (1:1,000, Abcam #ab176753) for 1h room temperature prior to slides mounting (Fluoromount/DAPI).
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bioRxiv - Cancer Biology 2024Quote: ... All of supernatant containing digested nuclear extract was transferred to a new tube and 500µl of Low IP buffer without additional NaCl or detergent was added together with 5-10µg of KAT7 antibody (Abcam #190908) or rabbit IgG (R&D Systems ...
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bioRxiv - Physiology 2024Quote: ... The membranes were blocked (5% milk (PanReac AppliChem, A0830)) and probed with the following antibodies: anti-β-Tubulin (Abcam, ab15568), anti-GAPDH (Proteintech ...
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bioRxiv - Immunology 2024Quote: ... Slides were washed 3 x 5 min and stained with 1:1 HRP-conjugated goat anti-rabbit IgG (Abcam ab214880) in 1% BSA-TBS for 2 hours at room temperature ...
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bioRxiv - Immunology 2024Quote: ... ∼50 µg of lysate material was treated with the reducing agent TCEP at a final concentration of 5 mM and heated to 60 °C for 30 minutes before being loaded onto a FASP protein digestion column (Abcam)75 ...
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bioRxiv - Bioengineering 2024Quote: ... the blots were washed with PBS supplemented with 0.1% Tween 20 four times for 5 min before staining with the secondary antibody (7076, Cell Signaling; ab216773, Abcam) in blocking buffer with 0.1% Tween 20 for 1h at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... cells were washed 3 times for 5 min with PBS before incubating with a secondary antibody (ab150080 and ab150157, Abcam) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then washed for 5 mins in PBS and mounted with mounting media containing DAPI (catalog number ab104139, Abcam) before imaging.
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bioRxiv - Bioengineering 2024Quote: ... for 5 minutes and then incubated for 1 hour at room temperature (RT) in NM95 primary antibody (ab190710 16 - Abcam) at 1:1500 in TBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were blocked with 5% milk-TBST for 1 hour under agitation and probed overnight for ALIX (1:1000, 186429, Abcam), Calnexin (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... The primary antibody was washed 2x with PBS for 5 min per wash and cells were incubated with secondary antibodies (diluted in 0.5% BSA/PBS) at room temperature in the dark for 1 h at 1:1000 dilution (anti-rabbit Alexa488, Abcam ab150077 ...