Labshake search
Citations for abcam :
2201 - 2250 of 2500 citations for 6 Methyl 5 propyl 4 1H pyrimidinone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... blocked with PBS/5% goat serum, and then incubated with primary antibodies: Mobp (1/1000, rabbit, Abgent) and Cnp (1/1000 mouse, Abcam) prior to exposure to fluorescent Alexa secondary antibodies ...
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bioRxiv - Microbiology 2022Quote: ... and incubated overnight with primary antibodies diluted in 5% BSA: rabbit anti-PCBP1 (clone EPR11055, Abcam ab168378, diluted 1:10,000); rabbit anti-actin (A2066 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then washed 8×5 min in PBS before being mounted onto glass slides and cover-slipped with fluoroshield mounting medium with DAPI (AbCam). Dual staining was performed with sequential primary-secondary incubations ...
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bioRxiv - Molecular Biology 2021Quote: ... Chromosome were blocked in PBT containing 0.2% BSA and 5% goat serum and sequentially incubated with primary antibodies (mouse anti-PolII H5 IgM, 1:1000, Abcam, and rat anti-HA MAb 3F10 ...
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bioRxiv - Microbiology 2020Quote: ... Membranes containing proteins were blocked in 5% BSA and incubated with rabbit anti-HMGB1 polyclonal antibody (1:1,000, Abcam, U.S.A), followed by incubation with horseradish peroxidase-linked secondary antibodies (1:5,000 ...
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bioRxiv - Genetics 2020Quote: ... Immunoprecipitation was performed by incubation of 2 ml diluted chromatin (equivalent to 5×106 input cells) with antibodies for H3K4me1 (3 µg ab195391, lot: GR304893-2; AbCam), H3K4me3 (1 µl 07-473 ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were blocked in 5% foetal bovine serum (FBS) for 30 min and incubated overnight in rabbit anti-PSD-95 (1:100; Abcam, Cambridge ...
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A novel neural stem cell-derived immunocompetent mouse model of glioblastoma for preclinical studiesbioRxiv - Cancer Biology 2020Quote: Formalin fixed paraffin-embedded sections (5 μm) were stained as described previously34 using the following primary antibodies: rabbit anti-Ki67 (ab15580, Abcam), rabbit anti-OLIG2 (ab109186 ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes incubated with the primary antibodies against claudin-5 (1:1000, Zymed 1600-34) or GAPDH (1:400, ab181602, ABCAM)) and the appropriate secondary antibodies.
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bioRxiv - Microbiology 2021Quote: Clonal lines were screened for knockout by labeling with primary mouse monoclonal antibodies to CD59 (clone MEM-43/5; Abcam) or CD46 (clone C-10 ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were blocked with 5% BSA in TBST then probed with primary antibodies for HMGCR (Ms mAb, 1:000, abcam), MVD (1:1000 ...
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bioRxiv - Plant Biology 2020Quote: ... The membrane was blocked with 5% non-fat skim milk and immunoblots were performed with indicated primary antibodies [anti-SUMO1 (Abcam), anti-Actin C3 (Abiocode) ...
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bioRxiv - Cell Biology 2021Quote: ... The membrane was washed and then incubated at RT for 1 h with horseradish-peroxidase (HRP)-conjugated goat anti-mouse (IgG) polyclonal antibody as the secondary antibody (1:5 000; Abcam). After three washes ...
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bioRxiv - Cell Biology 2020Quote: ... and heated to 95 °C for 5 min (no heating of the samples used for the total oxphos (abcam, ab110413) blots) ...
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bioRxiv - Neuroscience 2020Quote: ... They were then blocked with rotation at room temperature for an hour in blocking buffer (5% normal goat serum (Abcam), 0.2% TritonX-100 PBS) ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies were diluted in TBS + 2% BSA + 0.1% Triton X-100 at 5 µg/mL (rabbit anti-OCT4, Abcam #ab19857), 1:200 (mouse anti-NANOG ...
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bioRxiv - Physiology 2022Quote: ... blocked in 5% goat serum for 1 hour and stained using myelin protein zero (MPZ) (1 in 50 in 5% goat serum in PBS containing 1% triton) (EPR20383, Abcam) overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... Coverslips were then washed with 1xPBS 3x for 5 minutes before incubation in 50 µl spots of secondary antibodies (goat anti-mouse AF647: Abcam ab150115 ...
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bioRxiv - Neuroscience 2021Quote: ... The remaining supernatant was transferred to a 2 mL round-bottom tube and incubated with 5 µg/µl of anti-GFP antibody (ab290; Abcam) at 4°C with end-over-end rotation for one hour ...
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bioRxiv - Molecular Biology 2021Quote: ... the cells were permeabilized with 0.1% Triton X 100 in 1X PBS for 5 min and blocked with Protein Block (Abcam, USA) for 20 min at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Samples were blocked with 5% normal goat serum for 1 hr and incubated in anti-TRPM4 (Abcam, catalog no. ab106200) or normal mouse IgG (Santa Cruz Biotechnology ...
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bioRxiv - Immunology 2021Quote: ... 1×106 cells were blocked with DPBS with 5% of normal mouse serum (cat. n° ab7486, Abcam, Cambridge, MA, USA) for 30 min at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... 0.25 x 106 cells were seeded into 10 mL cell culture dishes then treated with 5 ng/mL TGFbeta (Abcam) on the next day ...
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bioRxiv - Immunology 2021Quote: ... The plates were then incubated with sequentially 1:5 serially diluted hACE2-Fc (invivogen) and two anti-SARS-CoV-2 RBD mAbs (clones H4 [InvivoGen] and CR3022 [Abcam]) starting at 10 μg/ml in blocking buffer ...
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bioRxiv - Systems Biology 2021Quote: ... All primary and secondary antibodies were diluted 1:400 in PBS containing either 2.25% bovine serum albumin [Millipore Sigma #A2058] or 5% normal goat serum (Abcam #ab7481) for blocking and 0.15% Triton X-100 (Sigma Aldritch #T8787 ...
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bioRxiv - Immunology 2022Quote: ... were washed with lysis buffer (containing protein inhibitors) and incubated with 5 µg of 4C4 antibody or IgG1k as a control isotype (ab18443 Abcam) at 4°C overnight under rotation ...
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bioRxiv - Synthetic Biology 2020Quote: ... 5 μL FLAG tag antibody (Anti-DDDDK-PE, Abcam ab72469, RRID: AB_1268475, or Anti-DDDDK-APC, Abcam ab72569, RRID: AB_1310127) was added at a concentration of 0.5 μg per sample and cells incubated at 4°C for 30 min ...
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bioRxiv - Pathology 2021Quote: ... The antigen was retrieved by incubating tissue sections for 5-7 minutes in Proteinase K antigen retrieval solution (Abcam, ab64220). Incubation with primary monoclonal antibodies against HCN1 (Novus Biologicals ...
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bioRxiv - Cell Biology 2021Quote: ... The remaining lysate was incubated with 5 μl of ssDNA (10mg/ml) and 2 µl of mouse monoclonal anti-V5 antibody (Abcam), 4 µl of rabbit polyclonal anti-PrA (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... coli were purchased and added to Kinase Buffer I (25 mM MOPS pH7.2, 12.5 mM β-glycerol phosphate, 25 mM MgCl2, 5 mM EGTA, 2 mM EDTA, 0.25 mM DTT; Abcam ab189135). Either water (mock ...
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bioRxiv - Immunology 2020Quote: ... serum was diluted 1:5 in diluent buffer and run in duplicate using Mouse LBP ELISA Kit (Abcam, Cambridge, MA) according to manufacturer’s instructions.
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bioRxiv - Physiology 2021Quote: ... Membranes were blocked in TBS-Tween 20 (0.1%) (TBST) with 5% BSA and then incubated overnight with primary antibodies for OXPHOS (Abcam MS604), P-RPS6 Ser235/236 (Cell Signaling ...
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bioRxiv - Cancer Biology 2021Quote: ... tissue sections were blocked with 5% mouse serum PBS1X for 1h30 and incubated with mouse anti-tryptase antibody (1:300, ab2378, RRID:AB_303023, ABcam, Cambridge, UK) for 1h at RT ...
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bioRxiv - Immunology 2020Quote: ... thawed unfixed tissue slices were rinsed with PBS then blocked with blocking solution (PBS supplemented with 2% Bovine Serum Albumin (Roth) and 5% Normal Goat Serum (Abcam)) for 1 hour at room temperature before incubation of mAbs at 5 µg/ml overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... ABCA1 (ATP-binding cassette lipid transporter marker of multivesicular bodies; mouse anti-human ABCA1; 5 μg/ml; Abcam Cat. #ab18180) and ...
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bioRxiv - Cell Biology 2020Quote: Exosomes (25 μg prot) from A-375 cells were incubated or not with 5 µl anti-CD63 antibodies (Abcam, ab8219) for 2 h at room temperature ...
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bioRxiv - Genomics 2021Quote: ... 500 µL of supernatant fluid was added to 25 µL of pre-incubated protein G magnetic beads (ChIP-Adembeads ChIP-Adem-Kit, Ademtech SA) with 5 µg of anti-V5 rabbit polyclonal antibody (ab9116, Abcam) in IP buffer (50 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Neuroscience 2021Quote: ... 10% DMSO) and put into primary antibody (PTwH, 5% DMSO, 3% donkey serum, Goat anti-GFP, Abcam ab5450, 1:1000) for 10 days at 37 degrees Celsius ...
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bioRxiv - Neuroscience 2022Quote: Immunohistochemistry was performed for neuroblast detection and analysis of hippocampal neurogenesis using the following primary antibodies diluted in PBS containing 5% goat serum and 0.1% Triton-X-100: anti-doublecortin (DCX, rabbit: 1:5000, Abcam, #ab18723), anti-bromodeoxyuridine (BrdU ...
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bioRxiv - Cancer Biology 2022Quote: ... The following antibodies were used with a total of 5 µg of each primary antibody used per immunoprecipitation: Rabbit anti-LSD1 (ab17721, Abcam), Rabbit ani-MYC (13987 ...
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bioRxiv - Cell Biology 2022Quote: ... Anti-Cullin 5(ab184177), anti-Elongin B antibody (ab154854, 1:2000) and anti-SOCS2 antibody (ab109245, 1:1000) were purchased from Abcam. Antibody binding was visualized with peroxidase-conjugated sheep anti-rabbit immunoglobulin (Southern Biotech ...
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Evaluation of the DBA/2J mouse as a potential background strain for genetic models of cardiomyopathybioRxiv - Physiology 2022Quote: ... blocked in 5% BSA-PBS at room temperature and incubated with the anti-syntrophin primary antibody (1:2000; #11425; Abcam) overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Tween-20 and 5% (w/v) milk powder containing 1:5,000 horseradish peroxidase-conjugated goat anti-mouse IgG secondary antibody (AbCam ab205719) and incubated on a shaking platform for 1 hr at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... 0.5 µm) were prepared and stained with 1% (w/v) toluidine blue and the Periodic Acid Schiff kit (ab150680, Abcam) as described in Hawkins et al ...
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bioRxiv - Neuroscience 2022Quote: ... They were then blocked with rotation at room temperature for an hour in blocking buffer (5% normal goat serum (Abcam), 0.2% TritonX-100 PBS) ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were washed 5 times with 1× PBST and incubated with goat anti-mouse horseradish peroxidase-conjugated secondary antibody (Abcam) for 30 mins then visualized using the chemiluminescence kit (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... Slides were then washed 3X in 0.4% Triton-X-100 TBS for 5 minutes before blocking at room temperature for 2 hours in a solution of 10% Normal Goat Serum (Abcam), 3% BSA (Fisher ...
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bioRxiv - Microbiology 2024Quote: ... 5% CO2 for 24 h before supernatants were collected and LDH levels determined using an LDH-Cytotoxicity Assay Kit (abcam).
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bioRxiv - Pathology 2024Quote: ... paraffin-embedded 5 µm slices were stained for insulin (ab195956, 1:1500, Alexa Fluor 555-conjugated secondary antibody: ab150186, 1:500, Abcam) and glucagon (ab92517 ...
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bioRxiv - Neuroscience 2024Quote: ... The slides were then incubated for 60 minutes at room temperature in blocking buffer (PBS with 5 % goat serum [Abcam #ab7481] and 0.3 % Triton X-100 [Bio-Rad Laboratories #161-0407]) ...