Labshake search
Citations for abcam :
2301 - 2350 of 2500 citations for 6 Methyl 5 propyl 4 1H pyrimidinone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Tissue sections were incubated over night at 4°C in blocking buffer containing rabbit anti-β III Tubulin (1:1000, Abcam, Cambridge, UK) for neurons detection ...
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bioRxiv - Genetics 2021Quote: ... ran side by side in a Criterion Precast Gradient 4-15% SDS-PAGE gel for western blotting probing of key intracellular organellar markers and proteins of interest: HSP60 (Abcam, cat. number ab46798), Calnexin (Abcam ...
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bioRxiv - Molecular Biology 2021Quote: ... Primary antibody incubations took place in a humid chamber at 4°C overnight with the following antibodies: chicken anti-GFP (Abcam ab13970 1:2000), mouse anti-mCherry (ab125096 1:1000) ...
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bioRxiv - Molecular Biology 2020Quote: ... BSA in PBS containing 0.1% Tween-20 and incubated overnight at 4°C with primary antibodies to TSG101 (1:1000; Abcam, Cambridge, UK, Cat. # ab30871), CD63 (1:500 ...
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bioRxiv - Bioengineering 2022Quote: ... Tissues were blocked overnight at 4°C in 20v/v% goat serum in PBS and then incubated with primary antibody solution (CD31, Abcam-ab28364, 1:50) overnight followed by goat anti-Rabbit Alexa Fluor 488® (Thermo Fisher Scientific ...
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bioRxiv - Pathology 2021Quote: ... The membrane was then probed overnight at 4°C with primary antibodies (P105/p50, 1:1000, Abcam ab32360; Actin, 1:1000, Abcam ab3280) in Odyssey Blocking Buffer and 0.1% Tween-20 ...
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bioRxiv - Neuroscience 2021Quote: ... The membrane was then incubated overnight on a shaker at 4 °C in blocking solution amended with the primary antibody [anti-Lamin B1 (Abcam; ab16048; 1:1000), anti-GAPDH (Abcam ...
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bioRxiv - Cell Biology 2021Quote: ... and for >16 hrs at 4°C with the following primary antibodies: mouse-α-CD63 (1:1000, clone TS63; Abcam, Cambridge, United Kingdom), mouse-α-Flotillin-1 (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... Chromatin (125 µg/IP) was incubated overnight in 2 µg of either rabbit polyclonal anti-histone 3 lysine 4 trimethyl (H3K4me3; Abcam, Cat# ab8580, RRID:AB_306649) or anti-Histone 3 (Abcam ...
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bioRxiv - Immunology 2020Quote: ... and mouse anti-human HSC70/HSP70 (clone N27F3-4, ENZO dilution 1:1.000, sample reduced with β-mercapthoethanol) and rabbit anti-human lactoferrin (polyclonal, Abcam, dilution 1:5.000), rabbit anti-human TLR3 (D10F10 ...
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bioRxiv - Neuroscience 2020Quote: ... bovine serum albumin (BSA) they incubated (4°C overnight) separately with specific primary antibodies including rabbit anti-tau polyclonal antibody (1:500, Abcam, Catalogue no. ab64193), rabbit anti GRP87/Bip polyclonal antibody (1:600 ...
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bioRxiv - Neuroscience 2021Quote: ... stained and incubated in a humidified chamber with rabbit anti-mouse Collagen I overnight at 4°C (1:100 dilution Abcam Catalog No. ab34710); washed 3 times in TBS-Tween (.05% Tween ...
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bioRxiv - Neuroscience 2022Quote: ... for 1 h at room temperature and were incubated overnight at 4 °C with primary rabbit polyclonal antibodies against mouse MAGL (Abcam, Cambridge, UK; ab180016) or human MAGL (LSBio ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.1% Triton X-100 in PBS at room temperature and incubated overnight at 4°C with primary antibodies: α-actinin (Abcam; 1:300), GLI2 (Novus biologicals ...
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bioRxiv - Molecular Biology 2022Quote: ... in PBST for 1 h at room temperature and then immunoblotted overnight at 4 °C with corresponding primary antibodies: anti-FTO (Abcam, ab124892, 1/1000) or anti-β-Actin (Sigma ...
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bioRxiv - Physiology 2022Quote: ... and then stained for αSMA overnight using rabbit anti-human αSMA antibody at a 1:500 dilution at 4°C (Abcam, San Francisco, CA). The chamber slides were incubated with a goat anti-rabbit secondary antibody (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... The membranes were incubated at 4°C overnight with the following primary antibodies: α- GADD153/CHOP (SCBT sc-166682) and α-Tubulin (Abcam ab176560). After washing ...
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bioRxiv - Biochemistry 2020Quote: ... blots were incubated overnight at 4°C with a mouse antibody against the α subunit of Na+/K+-ATPase (Abcam-ab7671, 1:2,000) and with a mouse antibody against the His-tag (Tetra·His Antibody ...
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bioRxiv - Pathology 2020Quote: ... membranes were probed against anti-citH3 or anti-PAD4 or anti-MPO or anti-p-ERK antibodies overnight at 4°C according to the manufacturer’s instructions (Abcam, UK and Massachusetts, USA). Membranes were washed extensively (4x ...
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bioRxiv - Immunology 2021Quote: ... in phosphate buffered saline (PBS) prior to overnight incubation at 4°C with blocking buffer containing primary antibodies raised against HIS48 (Abcam, Ab33760, 1:20), CD68 (Abcam ...
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bioRxiv - Microbiology 2022Quote: ... neutrophils on coverslips were fixed with 4% paraformaldehyde (PFA) and staining was performed with antibodies specific for H3Cit (citrulline R2 + R8 + R17, Abcam Ab5103, 1:200), myeloperoxidase (MPO ...
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bioRxiv - Molecular Biology 2022Quote: ... The membranes were blocked with 5% skimmed milk for 2 h at room temperature and then incubated with primary antibodies overnight at 4 °C as follows: anti-collagen I (ab270993; Abcam, USA; 1:1000), anti-α-smooth muscle actin (α-SMA ...
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bioRxiv - Molecular Biology 2022Quote: ... Plates were then incubated overnight at 4°C with primary antibodies (rabbit anti-IRF3 antibody [Cell Signaling Technology 11904S]; rabbit anti-G3BP1 antibody [Abcam ab181149]) diluted in PBS + 0.1% Triton X-100 (Sigma-Aldrich X100 ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was blocked with 5% skim milk for 1 hour at room temperature and then were incubated overnight at 4°C with the anti-MGST3 antibody (Abcam, ab192254; 1: 1000). Subsequently it was incubated with horseradish peroxidase (HRP)-conjugated secondary antibody (Cell signaling ...
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bioRxiv - Cancer Biology 2023Quote: ... Chondroitin sulfate (CS) and chondroitin 4-sulfate in the samples were determined following immunoprecipitation with CS antibody (CS-56, Abcam, Waltham, MA, USA), or C4S antibody LY111 (TCI Chemicals ...
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bioRxiv - Cell Biology 2023Quote: ... Fixed and blocked cover slips were subsequently incubated overnight at 4° C with a rabbit anti-collagen I (Abcam ab260043, 1:250 dilution) primary antibody ...
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bioRxiv - Neuroscience 2023Quote: ... for one hour and then stained overnight at 4 °C with primary antibody Chk pAb to GFP (Abcam Cat# ab13970, dilution 1:300). In the next day ...
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bioRxiv - Molecular Biology 2023Quote: ... Fixed cells were blocked in 1% BSA in PBS for 30 minutes at room temperature before overnight incubation at 4°C in primary antibodies: α-mouse CENP-A (1:1000 Abcam ab13939 (discontinued) or 1:1500 Enzo ADI-KAM-CC006-E ...
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bioRxiv - Cell Biology 2023Quote: ... The membranes were blocked in 5% skim milk solution for 1 h at RT then incubated with primary antibody at 4 ℃ overnight: Anti-FMRP antibody (Abcam, ab17722, 1:1,000), anti-GAPDH antibody (Cell signaling technology ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated with primary antibodies (ON; 4°C) that were appropriately diluted in blocking solution: chicken anti-GFP (Abcam; ab13970; 1:500), rabbit anti-GFP (Santa Cruz Biotechnology ...
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bioRxiv - Bioengineering 2024Quote: ... Incubation with the primary antibody was carried out at 4°C overnight in a PBS (1x) solution containing anti-Ki67 (Abcam, MA, 1:100), BSA (1%) ...
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bioRxiv - Cell Biology 2024Quote: ... for 1 hour at room temperature followed by overnight incubation at 4°C with primary antibody against TRP2/DCT (1:200, Abcam, catalog number ab74073). Alexa fluor-647 labeled secondary antibody (1:300 ...
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bioRxiv - Cancer Biology 2024Quote: ... 1000 µg of sonicated chromatin was then used per immunoprecipitation reaction and were incubated with 4 µg of H3K27ac antibody (4729, Abcam, Cambridge, MA, USA). Immunoprecipitated complexes were washed ...
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bioRxiv - Neuroscience 2024Quote: ... Tween-20 in tris-buffered saline (TBS-T) then incubated overnight at 4°C with one of the following primary antibodies: rabbit anti-GluK4 (Abcam; ab10101, 1:500), rabbit anti αB-crystallin (Enzo Life Sciences ...
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bioRxiv - Physiology 2023Quote: ... Membranes were blocked with Odyssey blocking buffer (Li-COR) and immunoblotted overnight (4°C) with rodent OXPHOS cocktail monoclonal antibody (Abcam; ab110413, 1:1000) to detect ETC proteins ...
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bioRxiv - Neuroscience 2023Quote: ... The slides with cryosectioned tissue were washed with PBS followed by incubation in blocking solution (2% of BSA in PBS) for 1 h and then incubated overnight at 4°C with an anti-DBI antibody (rabbit, 1:1000, Abcam, Cat. No: ab231910). Sections were then incubated for 1 h at room temperature with anti-rabbit Alex Fluor 546 (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... milk and the membranes incubated at 4°C overnight with rabbit Anti-Annexin A1/ANXA1 antibody monoclonal antibody [EPR19342]-BSA and Azide free (1: 25000, Abcam, Cat. N. ab222398), ALIX (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... Slides were then stained with the primary antibody diluted in 10% BSA in TBS-T overnight at 4 °C: FAP (Abcam, ab207178, 1:250) and POSTN (Abcam ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then incubated overnight at 4°C with primary antibodies directed against NP and GFP to amplify the Sec61ß-mEmerald signal (Abcam 13970, 1:500) in staining buffer (PBS supplemented with 0.2% Triton X-100 ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were incubated (overnight, at 4°C) with the primary antibody for detecting p-S129 aSyn (rabbit mAb EP1536Y Abcam, #ab51253-1:4000). Then ...
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bioRxiv - Pathology 2024Quote: ... The membrane was then probed overnight at 4°C with the primary antibodies (p105/p50, Abcam ab32360, 1:1000; Actin, Abcam ab3280, 1:1000) in Odyssey Blocking Buffer and 0.1% Tween-20 ...
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bioRxiv - Neuroscience 2024Quote: ... membranes were blocked in 5% skim milk diluted in PBS-T for 1 hr at room temperature and incubated overnight at 4°C with a rabbit synaptophysin primary antibody (YE269, Abcam, Cambridge, UK; #ab32127) diluted 1:2,000 in blocking solution ...
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bioRxiv - Neuroscience 2024Quote: ... at room temperature for 1 h and then incubated under agitation at 4° C overnight in a blocking solution containing the following primary antibodies: Rat anti-BrdU/CldU (Abcam, 1:1000, ab6326), and Mouse anti-IdU monoclonal antibody (Abcam ...
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bioRxiv - Biochemistry 2024Quote: ... A 2-hour blocking in 3% w/v non-fat milk in PBST was followed by overnight rocking at 4 °C with primary antibodies (anti-His-Abcam, Rabbit polyclonal (Ab137839) and anti-TULP3-Abcam ...
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bioRxiv - Biochemistry 2024Quote: ... overnight at 4°C and monoclonal mouse anti-human MUC5AC (Lidell et al., 2008)(1:200) in block solution over night at 4°C (Cat# ab3649, Abcam, Cambridge, UK, RRID:AB_2146844). Secondary antibody was polyclonal donkey anti-rabbit Alexa Fluor 488 (Cat# A-21206 ...
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bioRxiv - Bioengineering 2024Quote: ... in PBST for overnight at 4°C followed by PBS washing and incubation with FITC conjugated secondary antibody Goat Anti-Rabbit (IgG) (Abcam, U.K., 1:2000) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... injected with vehicle (VEH = 0.9% aqueous solution of NaCl and 5% ethanol) or MTZ (10 mg/kg, ab120068, Abcam, Cambridge, UK). The 10 mg/kg daily dosage in mice is equivalent to 50 mg/day in humans ...
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bioRxiv - Biochemistry 2020Quote: ... Membranes were treated with antibodies diluted 1:1000 in 0.5% BSA and washed 3 times for 5 min before applying secondary antibiotic (anti-mouse or anti-goat from Santa Cruz Biotechnology and Anti-Rabbit from Abcam at a concentration of 1:1000 ...
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bioRxiv - Immunology 2021Quote: ... The membrane was then washed 3×15 minutes in PBST and then incubated in secondary antibody solution (PBST + 5% BSA + Goat a-Rabbit-HRP 1:5000) (Abcam, ab205718) for one hour at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... blocked with 5% goat serum and incubated overnight with primary polyclonal rabbit antibodies in 5% goat serum at 1:150 dilution for types I (Abcam AB34710), II (Abcam AB34712) ...